ATE107357T1 - Verfahren zur verwendung von bar1 für die fremdproteinsekretion. - Google Patents

Verfahren zur verwendung von bar1 für die fremdproteinsekretion.

Info

Publication number
ATE107357T1
ATE107357T1 AT86114769T AT86114769T ATE107357T1 AT E107357 T1 ATE107357 T1 AT E107357T1 AT 86114769 T AT86114769 T AT 86114769T AT 86114769 T AT86114769 T AT 86114769T AT E107357 T1 ATE107357 T1 AT E107357T1
Authority
AT
Austria
Prior art keywords
bar1
methods
foreign protein
foreign
protein secretion
Prior art date
Application number
AT86114769T
Other languages
English (en)
Inventor
Vivian L Mackay
Original Assignee
Zymogenetics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/US1986/002198 external-priority patent/WO1987002670A1/en
Application filed by Zymogenetics Inc filed Critical Zymogenetics Inc
Application granted granted Critical
Publication of ATE107357T1 publication Critical patent/ATE107357T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/10—Fusion polypeptide containing a localisation/targetting motif containing a tag for extracellular membrane crossing, e.g. TAT or VP22
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Mycology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Peptides Or Proteins (AREA)
AT86114769T 1985-10-25 1986-10-24 Verfahren zur verwendung von bar1 für die fremdproteinsekretion. ATE107357T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US79130585A 1985-10-25 1985-10-25
PCT/US1986/002198 WO1987002670A1 (en) 1985-10-25 1986-10-20 Method of using bar1 for secreting foreign proteins

Publications (1)

Publication Number Publication Date
ATE107357T1 true ATE107357T1 (de) 1994-07-15

Family

ID=26774053

Family Applications (1)

Application Number Title Priority Date Filing Date
AT86114769T ATE107357T1 (de) 1985-10-25 1986-10-24 Verfahren zur verwendung von bar1 für die fremdproteinsekretion.

Country Status (5)

Country Link
EP (1) EP0220689B1 (de)
AT (1) ATE107357T1 (de)
DE (1) DE3689918T2 (de)
ES (1) ES2056785T3 (de)
RU (1) RU2091490C1 (de)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3887425T2 (de) * 1987-10-02 1994-06-23 Zymogenetics Inc BAR1-Sekretions-Signal-Sequenz.
EP0387319B1 (de) * 1988-07-23 1996-03-06 Delta Biotechnology Limited Sekretorische leader-sequenzen
US6348327B1 (en) 1991-12-06 2002-02-19 Genentech, Inc. Non-endocrine animal host cells capable of expressing variant proinsulin and processing the same to form active, mature insulin and methods of culturing such cells
FI92601C (fi) * 1992-03-11 1994-12-12 Marja Makarow Menetelmä hyötyproteiinien erittämiseksi hiivoista
IT1255697B (it) * 1992-09-29 1995-11-10 Enichem Spa Metodo per l'espressione nel lievito schizosaccharomyces pombe di recettori del sistema nervoso umano
DE4441197C1 (de) * 1994-11-18 1996-03-28 Deutsches Krebsforsch Expressionsplasmide für Hefe
CA2198966C (en) * 1996-03-04 2011-06-21 Yuji Suzuki Method for cleaving chimeric protein using processing enzyme
RU2238951C2 (ru) * 1998-03-31 2004-10-27 Тонгхуа Гэнтеч Биотекнолоджи Лтд. Химерный белок, обеспечивающий образование биоактивной конформации, способ получения белка с инсулиновой активностью, способ идентификации пептидильного фрагмента
BR0014059B1 (pt) 1999-09-30 2013-02-05 processo para produzir transglutaminase.
RU2224796C2 (ru) * 1999-09-30 2004-02-27 Адзиномото Ко., Инк. Способ получения гетерологичного секреторного белка
JP4056880B2 (ja) * 2000-12-14 2008-03-05 ジェネンテック・インコーポレーテッド 細菌性宿主株
CN1250722C (zh) * 2001-03-30 2006-04-12 味之素株式会社 分泌生产蛋白质的方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK172738B1 (da) * 1983-04-07 1999-06-21 Chiron Corp Fremgangsmåde til at udtrykke modent insulin og DNA-struktur til anvendelse ved fremgangsmåden
US4613572A (en) * 1983-08-15 1986-09-23 Kansas State University Research Foundation Yeast BAR1 gene plasmid
DE3537176C2 (de) * 1984-10-18 1994-06-09 Zymogenetics Inc Gewebeplasminogenaktivator und Verfahren zu seiner Herstellung

Also Published As

Publication number Publication date
EP0220689A2 (de) 1987-05-06
DE3689918D1 (de) 1994-07-21
DE3689918T2 (de) 1994-10-20
EP0220689B1 (de) 1994-06-15
EP0220689A3 (en) 1988-08-31
RU2091490C1 (ru) 1997-09-27
ES2056785T3 (es) 1994-10-16

Similar Documents

Publication Publication Date Title
ATE107357T1 (de) Verfahren zur verwendung von bar1 für die fremdproteinsekretion.
ATE77104T1 (de) Menge und verfahren zur herstellung rekombinierter erzeugnisse in grosser ausbeute.
AU2723384A (en) Production of human insulin-like growth factor through biogenetic techniques
ES544868A0 (es) Procedimiento de obtencion de una proteina de factor estimu-lante de colonias (csf) en primates
IE883896L (en) Improved expression and secretion of heterologous proteins in yeast employing truncated alpha-factor leader sequences
EP0228254A3 (de) Hefe-Sekretionsvektoren
EP0145390A3 (de) Methode zur Herstellung von nicht-glykosyliertem humanen Interleukin-2 Protein
FI864206A0 (fi) Menetelmä heterologisten proteiinien tuottamiseksi Yarrowia lipolytica -transformanttien avulla, menetelmä Yarrowia lipolytica -transformanttien valmistamiseksi ja toteamiseksi sekä menetelmä transformanttien valmistamiseen käyttökelpoisten plasmidien valmistamiseksi
NO180593C (no) Fremgangsmåte for fremstilling, i Saccharomyces cerevisiae, av et heterologt protein i homogen form
KR970065558A (ko) 분비형 Kex2 유도체의 제조방법
FR2645174B1 (fr) Souches de levure ameliorees pour la production de proteines heterologues matures en particulier d'hirudine et procede de preparation d'hirudine correspondant
Hiramatsu et al. The secretion leader of Mucor pusillus rennin which possesses an artificial Lys-Arg sequence directs the secretion of mature human growth hormone by Saccharomyces cerevisiae
DE3687112D1 (de) Verfahren zur ausscheidung von genprodukten in das wachstumsmedium von gram-negativen bakterien.
DK0663443T4 (da) Dextranaseenzym, fremgangsmåde til fremstilling deraf og DNA, der koder for enzymet
EP0422217A4 (en) Human recombinant placental ribonuclease inhibitor and method of production
JPS6443193A (en) Production of phospholipase a2
EP0184169A3 (de) Verfahren zur Herstellung von heterologen Proteinen
EP0165759A3 (de) Säugetierpromotoren verwendbar in Hefeexpression
KR950032618A (ko) 인간 리포코틴 분비 생산을 위한 효모용 발현 벡터
KR860002569A (ko) 인체인슐린-유사생장인자의 제조방법

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
REN Ceased due to non-payment of the annual fee