ATE107701T1 - Expression und sekretion von reifem menschlichem beta-interleukin-1 in bacillus subtilis und mittel und verfahren zu deren ausführung. - Google Patents

Expression und sekretion von reifem menschlichem beta-interleukin-1 in bacillus subtilis und mittel und verfahren zu deren ausführung.

Info

Publication number
ATE107701T1
ATE107701T1 AT90113414T AT90113414T ATE107701T1 AT E107701 T1 ATE107701 T1 AT E107701T1 AT 90113414 T AT90113414 T AT 90113414T AT 90113414 T AT90113414 T AT 90113414T AT E107701 T1 ATE107701 T1 AT E107701T1
Authority
AT
Austria
Prior art keywords
human beta
secretion
expression
interleukin
mature human
Prior art date
Application number
AT90113414T
Other languages
English (en)
Inventor
Bellini Ada Velati
Giuliano Galli
Giuseppe Lucchese
Guido Grandi
Original Assignee
Eniricerche Spa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eniricerche Spa filed Critical Eniricerche Spa
Application granted granted Critical
Publication of ATE107701T1 publication Critical patent/ATE107701T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/545—IL-1
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/832—Bacillus
    • Y10S435/839—Bacillus subtilis

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
AT90113414T 1989-07-19 1990-07-13 Expression und sekretion von reifem menschlichem beta-interleukin-1 in bacillus subtilis und mittel und verfahren zu deren ausführung. ATE107701T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IT8921228A IT1231156B (it) 1989-07-19 1989-07-19 Espressione e secrezione di interleuchina 1 beta umana matura in bacillus subtilis e mezzi e metodi per il suo ottenimento.

Publications (1)

Publication Number Publication Date
ATE107701T1 true ATE107701T1 (de) 1994-07-15

Family

ID=11178709

Family Applications (1)

Application Number Title Priority Date Filing Date
AT90113414T ATE107701T1 (de) 1989-07-19 1990-07-13 Expression und sekretion von reifem menschlichem beta-interleukin-1 in bacillus subtilis und mittel und verfahren zu deren ausführung.

Country Status (8)

Country Link
US (2) US5437988A (de)
EP (1) EP0409098B1 (de)
AT (1) ATE107701T1 (de)
CA (1) CA2021406A1 (de)
DE (1) DE69010096T2 (de)
DK (1) DK0409098T3 (de)
ES (1) ES2058695T3 (de)
IT (1) IT1231156B (de)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1270866B (it) * 1993-03-10 1997-05-13 Eniricerche Spa Vettore ricombinante e suo impiego per la preparazione esocellulare di anticorpi in forma di singola molecola da bacillus subtilis
US6270758B1 (en) 1998-10-08 2001-08-07 Duke University Substantially non-toxic biologically active mucosal adjuvants in vertebrate subjects
BR0210252A (pt) 2001-07-13 2004-09-14 Pepharm R & D Ltd Peptìdeos biologicamente ativos
EP1627885B8 (de) 2003-04-30 2015-02-18 Innovative Medicine and Any Peptide Pharmaceuticals Corp Polypeptid, das dazu in der lage ist, die absorption von verdauungsorganen zu unterstützen
TWI353252B (en) 2004-04-28 2011-12-01 Cms Peptides Patent Holding Company Ltd Biologically active peptide vapeehptllteaplnpk der
US20070061899A1 (en) * 2005-04-13 2007-03-15 The Trustees Of Columbia University In The City Of New York Transgenic mammal with constitutive, tissue-specific cytokine expression and use as a cancer model
NZ591825A (en) 2005-07-26 2012-10-26 Cms Peptides Patent Holding Company Ltd Biologically active peptides comprising PTTKTYFPHF and their uses in the manufacture of a medicament for modulating fatigue
CA2774636C (en) 2009-09-25 2019-05-21 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Neutralizing antibodies to hiv-1 and their use
EP2788376B1 (de) 2011-12-08 2018-10-03 The United States of America, as represented by The Secretary, Department of Health and Human Services Neutralisierende antikörper gegen hiv-1 und ihre verwendung
WO2014126921A1 (en) 2013-02-12 2014-08-21 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Monoclonal antibodies that neutralize norovirus
EP2994163B1 (de) 2013-05-09 2019-08-28 The United States of America, as represented by The Secretary, Department of Health and Human Services Single-domain-vhh-antikörper gegen norovirus gi.1 und gii.4 und deren verwendung
WO2016090170A1 (en) 2014-12-05 2016-06-09 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services A potent anti-influenza a neuraminidase subtype n1 antibody
GB201710838D0 (en) 2017-07-05 2017-08-16 Ucl Business Plc Bispecific antibodies

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE251158C (de) *
GB2171703B (en) * 1985-01-18 1989-11-15 Agency Ind Science Techn Expression-secretion vector for gene expression and protein secretion, recombinant dna including the vector, and method of producing proteins by use of the re
IT1186750B (it) * 1985-07-10 1987-12-16 Eniricerche Spa Vettore di clonaggio,molecole di dna ricombinante,ceppi di bacillus subtilis trasformati con dette molecole e metodi per l'espressione di geni eterologhi e produzione e secrezione di proteine codificate da detti geni
JPH0669377B2 (ja) * 1985-11-25 1994-09-07 工業技術院長 Ｄｎａ塩基配列およびベクタ−
IT1196484B (it) * 1986-07-11 1988-11-16 Sclavo Spa Vettore ad espressione e secrezione in lieviti,utile per la preparazione di proteine eterologhe
DD251158A1 (de) * 1986-07-17 1987-11-04 Adw Ddr Verfahren zur herstellung von streptokokken-vektorplasmiden mit polylinker
IT1228925B (it) * 1987-08-07 1991-07-10 Eniricerche Spa Procedimento per la preparazione dell'ormone della crescita umano
IT1226713B (it) * 1988-08-05 1991-02-05 Eniricerche Spa Procedimento per la preparazione di interleuchina 1 beta umana ricombinante in forma omogenea.

Also Published As

Publication number Publication date
ES2058695T3 (es) 1994-11-01
EP0409098B1 (de) 1994-06-22
IT8921228A0 (it) 1989-07-19
CA2021406A1 (en) 1991-01-20
DE69010096D1 (de) 1994-07-28
US5728571A (en) 1998-03-17
DK0409098T3 (da) 1994-07-25
EP0409098A1 (de) 1991-01-23
IT1231156B (it) 1991-11-19
US5437988A (en) 1995-08-01
DE69010096T2 (de) 1994-10-13

Similar Documents

Publication Publication Date Title
ATE107701T1 (de) Expression und sekretion von reifem menschlichem beta-interleukin-1 in bacillus subtilis und mittel und verfahren zu deren ausführung.
DK0400958T3 (da) Levende vacciner
FI893702A0 (fi) Foerfarande foer mikrobiologisk framstaellning av humant serumalbumin och andra heterologa proteiner ur jaest.
AU561343B2 (en) Human immune interferon by recombinant dna
PT83761B (pt) Metodo para a producao de interleuquina-4 de mamifero
IL81018A (en) Fusion proteins comprising small fragments of human interleukin-2 as ballast,process for its preparation and genes,vectors and transformed host containing said fragments and used in the process
EP0634936A4 (de) Materialien und methoden für die immunopräzipitation.
DE69533102D1 (de) Analog von haemophilus hin47 mit verminderter protease aktivität
DK0425082T3 (da) Vacciner
AU8258591A (en) Human mk gene and protein sequence
NO902251D0 (no) Fremstilling av gonorrheiske p1-proteiner og vaksiner.
DE3879823D1 (de) Verfahren zur herstellung von menschlichem wachstumshormon.
DE69014541D1 (de) Chemisch modifizierte Allergene und Verfahren zu ihrer Herstellung.
AU601415B2 (en) A process for the purification, solubilization and/or detoxification of protein antigens of bacteria of the bordetella genus using a carbonate buffer and an acellular anti-whooping cough vaccine
DE3686961D1 (de) Polydisperse native pseudomonas-flagella(h)-antigene (fag) und verfahren zu ihrer gewinnung.
DK292584A (da) Biologisk aktive konjugater, deres fremstilling og anvendelse
AU1540795A (en) Diagnosis and treatment of infections due to streptococci and enterococci
DE3889562D1 (de) Rekombinante Plasmide, die zur Expression von heterologen Proteinen in Bacillus verwendet werden können.
Bellini et al. Expression and secretion of mature human beta interleukin-1 in bacillus subtilis and means and methods for its achievement
ATE111522T1 (de) Methode zur herstellung von rekombinantem menschlichem beta-interleukin-1 in homogener form.

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
REN Ceased due to non-payment of the annual fee