ATE110772T1 - Mutierter t-pa mit kringle-ersatz. - Google Patents

Mutierter t-pa mit kringle-ersatz.

Info

Publication number
ATE110772T1
ATE110772T1 AT88108949T AT88108949T ATE110772T1 AT E110772 T1 ATE110772 T1 AT E110772T1 AT 88108949 T AT88108949 T AT 88108949T AT 88108949 T AT88108949 T AT 88108949T AT E110772 T1 ATE110772 T1 AT E110772T1
Authority
AT
Austria
Prior art keywords
analogs
kringle
fibrin
native
disclosed
Prior art date
Application number
AT88108949T
Other languages
English (en)
Inventor
Eileen R Mulvihill
Bjorn A Nexo
Shinji Yoshitake
Yasunori Ikeda
Suguru Suzuki
Akira Hashimoto
Teruaki Yuzuriha
Original Assignee
Zymogenetics Inc
Novo Nordisk As
Eisai Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zymogenetics Inc, Novo Nordisk As, Eisai Co Ltd filed Critical Zymogenetics Inc
Application granted granted Critical
Publication of ATE110772T1 publication Critical patent/ATE110772T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Hematology (AREA)
  • Diabetes (AREA)
  • Veterinary Medicine (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Semiconductor Lasers (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
  • Silicon Polymers (AREA)
  • Oscillators With Electromechanical Resonators (AREA)
  • Saccharide Compounds (AREA)
AT88108949T 1987-06-04 1988-06-03 Mutierter t-pa mit kringle-ersatz. ATE110772T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US5821787A 1987-06-04 1987-06-04

Publications (1)

Publication Number Publication Date
ATE110772T1 true ATE110772T1 (de) 1994-09-15

Family

ID=22015426

Family Applications (1)

Application Number Title Priority Date Filing Date
AT88108949T ATE110772T1 (de) 1987-06-04 1988-06-03 Mutierter t-pa mit kringle-ersatz.

Country Status (14)

Country Link
EP (1) EP0293934B1 (de)
JP (1) JPS6485078A (de)
KR (1) KR970005251B1 (de)
AT (1) ATE110772T1 (de)
AU (1) AU617323B2 (de)
CA (1) CA1341447C (de)
DE (1) DE3851259T2 (de)
DK (1) DK302288A (de)
ES (1) ES2058180T3 (de)
FI (1) FI882628L (de)
IE (1) IE64782B1 (de)
NO (1) NO179754C (de)
NZ (1) NZ224915A (de)
ZA (1) ZA883958B (de)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5187098A (en) * 1984-03-02 1993-02-16 Board Of Regents Of The University Of Oklahoma DNA encoding hybrid streptokinases with plasminogen fibrin binding domains
US5756093A (en) * 1985-04-22 1998-05-26 Genentech, Inc. Tissue plasminogen activator variants
US5736134A (en) * 1985-04-22 1998-04-07 Genentech, In.C Tissue plasminogen activator variants
US5714372A (en) * 1985-04-22 1998-02-03 Genentech, Inc. Tissue plasminogen activator variants
US5302390A (en) * 1987-07-01 1994-04-12 Beecham Group Plc Hybrid proteins of human plasminogen and human t-PA, pharmaceutical compositions and methods of treatment
US5258180A (en) * 1988-09-02 1993-11-02 Genetech, Inc. Tissue plasminogen activator having fibrin specific properties and deletion of amino acids 466-970, compositions and methods of treatment
US5108901A (en) * 1988-09-02 1992-04-28 Genentech, Inc. Tissue plasminogen activator having zymogenic or fibrin specific properties
US5714145A (en) * 1988-09-02 1998-02-03 Genentech, Inc. Tissue plasminogen activator having zymogenic or fibrin specific properties
US5262170A (en) * 1988-09-02 1993-11-16 Genentech, Inc. Tissue plasminogen activator having zymogenic or fibrin specific properties and substituted at amino acid positions 296-299, DNA molecules encoding them, vectors, and host cells
DE3836200A1 (de) * 1988-10-24 1990-04-26 Boehringer Mannheim Gmbh Domaenenduplikations-muteine des gewebsplasminogen-aktivators (t-pa)
GB8901422D0 (en) * 1989-01-23 1989-03-15 Fujisawa Pharmaceutical Co New tissue plasminogen activator
US5190756A (en) * 1989-12-01 1993-03-02 Genentech, Inc. Methods and materials for expression of human plasminogen variant
US5087572A (en) * 1989-12-01 1992-02-11 Genentech, Inc. Dna encoding human plasminogen modified at the cleavage site
GB9019120D0 (en) * 1990-09-01 1990-10-17 Beecham Group Plc Novel compounds
US5272076A (en) * 1991-06-13 1993-12-21 Eli Lilly And Company Compounds and methods for treatment of thromboembolic disorders using an adduct of t-PA
EP0618964A4 (en) * 1991-12-16 1995-12-06 Genentech Inc Novel tissue plasminogen activator variants.
PT786257E (pt) * 1992-06-03 2003-12-31 Genentech Inc Variantes de glicosilacao do activador de plasminogenio tissular com propriedades terapeuticas meloradas
US6706504B1 (en) 1996-11-12 2004-03-16 The Scripps Research Institute Tissue type plasminogen activator (t-PA) variants: compositions and methods of use

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1984001786A1 (en) * 1982-10-28 1984-05-10 Beecham Group Plc Enzyme derivatives and their use in the treatment of thrombosis
EP0207589A1 (de) * 1985-04-03 1987-01-07 Beecham Group Plc Modifiziertes fibrinolytisches Enzym
IE58574B1 (en) * 1985-04-22 1993-10-06 Genentech Inc Human tissue plasminogen activator mutants, methods and intermediates therefor, and compositions using such mutants
IE81073B1 (en) * 1986-03-18 2000-01-12 Genentech Inc Modified human tissue-type plasminogen activator and its preparation
PT84588B (en) * 1986-04-02 1989-05-09 Beecham Group Plc Process for preparing a fibrinolytic enzyme
DE3643158A1 (de) * 1986-04-21 1987-11-19 Boehringer Mannheim Gmbh Gewebs-plasminogen-aktivator(tpa) - derivat und seine herstellung
FI101306B (fi) * 1987-06-04 1998-05-29 Zymogenetics Inc Menetelmä kudostyyppisen plasminogeeniaktivaattorin analogien, joissa on muokatut kasvutekijädomeenit, valmistamiseksi

Also Published As

Publication number Publication date
ZA883958B (en) 1989-07-26
DK302288A (da) 1989-02-03
EP0293934A1 (de) 1988-12-07
FI882628A7 (fi) 1988-12-05
EP0293934B1 (de) 1994-08-31
ES2058180T3 (es) 1994-11-01
FI882628L (fi) 1988-12-05
DK302288D0 (da) 1988-06-03
DE3851259D1 (de) 1994-10-06
IE881681L (en) 1988-12-04
NO882453L (no) 1988-12-05
DE3851259T2 (de) 1994-12-15
NO179754C (no) 1996-12-11
JPS6485078A (en) 1989-03-30
KR970005251B1 (ko) 1997-04-14
AU1741088A (en) 1988-12-08
CA1341447C (en) 2003-11-18
NO882453D0 (no) 1988-06-03
AU617323B2 (en) 1991-11-28
JPH0448433B2 (de) 1992-08-06
KR890000665A (ko) 1989-03-16
IE64782B1 (en) 1995-09-06
FI882628A0 (fi) 1988-06-03
NZ224915A (en) 1989-12-21
NO179754B (no) 1996-09-02

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Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
EELA Cancelled due to lapse of time