ES2091224T3 - Subtilisinas con mutaciones multiples. - Google Patents

Subtilisinas con mutaciones multiples.

Info

Publication number
ES2091224T3
ES2091224T3 ES90304715T ES90304715T ES2091224T3 ES 2091224 T3 ES2091224 T3 ES 2091224T3 ES 90304715 T ES90304715 T ES 90304715T ES 90304715 T ES90304715 T ES 90304715T ES 2091224 T3 ES2091224 T3 ES 2091224T3
Authority
ES
Spain
Prior art keywords
subtilisins
multiple mutations
subtilisin
admixture
expressing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90304715T
Other languages
English (en)
Inventor
Mark M Zukowski
Linda O Narhi
Michael Levitt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Amgen Inc
Original Assignee
Amgen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amgen Inc filed Critical Amgen Inc
Application granted granted Critical
Publication of ES2091224T3 publication Critical patent/ES2091224T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Detergent Compositions (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Cosmetics (AREA)
  • Tires In General (AREA)

Abstract

UNA CLASE DE ANALOGOS DE LA SUBTILISINA ADECUADA PARA SER MEZCLADA CON COMPOSICIONES DE LIMPIEZA Y CON UNA ESTABILIDAD MEJORADA SOBRE EL BACILO DE LA SUBTISILINA. ESTOS ANALOGOS SON PREPARADOS EXPRESANDO UN GEN MODIFICADO CODIFICADOR DEL ANALOGO DE LA SUBTILISINA EN EL BACILLUS SUBTILIS.
ES90304715T 1989-05-17 1990-05-01 Subtilisinas con mutaciones multiples. Expired - Lifetime ES2091224T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US35312489A 1989-05-17 1989-05-17

Publications (1)

Publication Number Publication Date
ES2091224T3 true ES2091224T3 (es) 1996-11-01

Family

ID=23387862

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90304715T Expired - Lifetime ES2091224T3 (es) 1989-05-17 1990-05-01 Subtilisinas con mutaciones multiples.

Country Status (13)

Country Link
US (1) US5397705A (es)
EP (1) EP0398539B1 (es)
JP (1) JPH03505976A (es)
KR (1) KR100188532B1 (es)
AT (1) ATE141324T1 (es)
AU (1) AU618675B2 (es)
CA (1) CA2016211C (es)
DE (1) DE69028040T2 (es)
DK (1) DK0398539T3 (es)
ES (1) ES2091224T3 (es)
GR (1) GR3021010T3 (es)
SG (1) SG47797A1 (es)
WO (1) WO1990014420A1 (es)

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988008028A1 (en) * 1987-04-06 1988-10-20 Genex Corporation The engineering of electrostatic interactions at metal ion binding sites for the stabilization of proteins
US5665587A (en) * 1989-06-26 1997-09-09 Novo Nordisk A/S Modified subtilisins and detergent compositions containing same
DK271490D0 (da) * 1990-11-14 1990-11-14 Novo Nordisk As Detergentkomposition
US5863783A (en) * 1991-03-27 1999-01-26 Gist-Brocades, N.V. Cloning and expression of DNA molecules encoding arabinan-degrading enzymes of fungal origin
BR9206636A (pt) * 1991-10-16 1995-10-24 Unilever Nv Composição detergente enzimática e processo para a preparação de uma composição detergente enzimática aquosa
US5989887A (en) * 1992-11-25 1999-11-23 Dsm N.V. Cloning and expression of DNA molecules incoding arabinan-degrading enzymes of fungal origin
US5567601A (en) * 1993-06-01 1996-10-22 University Of Maryland Subtilisin mutants lacking a primary calcium binding site
US6440717B1 (en) 1993-09-15 2002-08-27 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis
US6436690B1 (en) 1993-09-15 2002-08-20 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
DE69434962T2 (de) * 1993-10-14 2008-01-17 The Procter & Gamble Company, Cincinnati Proteasehaltige reinigungsmittel
MA23346A1 (fr) * 1993-10-14 1995-04-01 Genencor Int Variantes de la subtilisine
ES2199978T3 (es) * 1993-10-14 2004-03-01 THE PROCTER & GAMBLE COMPANY Composiciones de blanqueo que comprenden enzimas proteasa.
DE4411223A1 (de) * 1994-03-31 1995-10-05 Solvay Enzymes Gmbh & Co Kg Verwendung alkalischer Proteasen in gewerblichen Textilwaschverfahren
US6599730B1 (en) 1994-05-02 2003-07-29 Procter & Gamble Company Subtilisin 309 variants having decreased adsorption and increased hydrolysis
US6066611A (en) * 1994-10-13 2000-05-23 The Procter & Gamble Company Bleaching compositions comprising protease enzymes
US6455295B1 (en) 1995-03-08 2002-09-24 The Procter & Gamble Company Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis
IL117350A0 (en) 1995-03-09 1996-07-23 Procter & Gamble Proteinase k variants having decreased adsorption and increased hydrolysis
US6475765B1 (en) 1995-03-09 2002-11-05 Procter & Gamble Company Subtilisin DY variants having decreased adsorption and increased hydrolysis
EP0946704B1 (en) * 1996-12-20 2003-03-19 Unilever N.V. Enzymatic bleach composition
US6204234B1 (en) * 1997-07-09 2001-03-20 The Proctor & Gamble Company Cleaning compositions comprising a specific oxygenase
AR016969A1 (es) * 1997-10-23 2001-08-01 Procter & Gamble VARIANTE DE PROTEASA, ADN, VECTOR DE EXPRESIoN, MICROORGANISMO HUESPED, COMPOSICIoN DE LIMPIEZA, ALIMENTO PARA ANIMALES Y COMPOSICIoN PARA TRATAR UN TEXTIL
ES2367505T3 (es) * 1997-10-23 2011-11-04 Danisco Us Inc. Variantes de proteasa con sustituciones múltiples con carga neta alterada para usar en detergentes.
BR9909130A (pt) 1998-03-26 2000-12-19 Procter & Gamble Variantes de protease de serina tendo substituições de amino-ácido
US6908757B1 (en) 1998-03-26 2005-06-21 The Procter & Gamble Company Serine protease variants having amino acid deletions and substitutions
BR0012694A (pt) 1999-07-22 2002-04-09 Procter & Gamble Conjugado de protease, composição de limpeza e composição de tratamento pessoal
CA2376045A1 (en) 1999-07-22 2001-02-01 The Procter & Gamble Company Subtilisin protease variants having amino acid substitutions in defined epitope regions
US6946128B1 (en) 1999-07-22 2005-09-20 The Procter & Gamble Company Protease conjugates having sterically protected epitope regions
KR20020021397A (ko) 1999-07-22 2002-03-20 데이비드 엠 모이어 규정된 에피토프 영역에 아미노산 결실 및 치환을 갖는서브틸리신 프로테아제 변이체
NZ517409A (en) 1999-08-31 2004-05-28 Novozymes As RP-II properties with amino acid substitutions used in detergent compositions and additives
US7217554B2 (en) 1999-08-31 2007-05-15 Novozymes A/S Proteases and variants thereof
US6727085B2 (en) * 1999-12-15 2004-04-27 Fanoe Tina Sejersgaard Subtilase variants having an improved wash performance on egg stains
US7087415B2 (en) * 2000-07-31 2006-08-08 Athena Biotechnologies, Inc. Methods and compositions for directed gene assembly
AU2002256624B2 (en) 2001-02-19 2007-12-06 Merck Patent Gmbh Method for identification of T-cell epitopes and use for preparing molecules with reduced immunogenicity
PL210859B1 (pl) * 2001-03-23 2012-03-30 Genencor Int Odmiana subtylizyny zawierająca zmieniony epitop limfocytów T, kwas nukleinowy kodujący tę odmianę subtylizyny, wektor ekspresyjny i komórka gospodarza zawierająca kwas nukleinowy oraz kompozycje zawierające tę odmianę subtylizyny
US20050227229A1 (en) * 2001-07-09 2005-10-13 Lebo Roger V Multiple controls for molecular genetic analyses
DE10153792A1 (de) 2001-10-31 2003-05-22 Henkel Kgaa Neue Alkalische Protease-Varianten und Wasch- und Reinigungsmittel enthaltend diese neuen Alkalischen Protease-Varianten
WO2003062380A2 (en) * 2002-01-16 2003-07-31 Genencor International, Inc. Multiply-substituted protease variants
US7888093B2 (en) * 2002-11-06 2011-02-15 Novozymes A/S Subtilase variants
US7294499B2 (en) 2003-01-30 2007-11-13 Novozymes A/S Subtilases
US7727756B2 (en) * 2003-03-21 2010-06-01 Novozymes A/S Subtilases
CN102766545B (zh) * 2003-05-07 2014-08-06 诺维信公司 变体枯草杆菌蛋白酶（枯草杆菌酶）
EP3887515A1 (en) * 2018-11-28 2021-10-06 Danisco US Inc. Subtilisin variants having improved stability
US20250311734A1 (en) 2022-05-14 2025-10-09 Novozymes A/S Compositions and Methods for Preventing, Treating, Suppressing and/or Eliminatting Phytopathogenic Infestations and Infections

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4760025A (en) * 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
US5155033A (en) * 1989-01-06 1992-10-13 Genencor, Inc. Subtilisins modified at position 225 resulting in a shift in catalytic activity
WO1987004461A1 (en) * 1986-01-15 1987-07-30 Amgen THERMALLY STABLE AND pH STABLE SUBTILISIN ANALOGS AND METHOD FOR PRODUCTION THEREOF
US5013657A (en) * 1988-04-12 1991-05-07 Bryan Philip N Subtilisin mutations
US4980288A (en) * 1986-02-12 1990-12-25 Genex Corporation Subtilisin with increased thermal stability
US4990452A (en) * 1986-02-12 1991-02-05 Genex Corporation Combining mutations for stabilization of subtilisin
DE3750916T2 (de) * 1986-04-30 1995-06-01 Genencor Int Mutante einer nicht menschlichen Carbonyl-Hydrolase, für diese kodierende DNS-Sequenzen und Vektoren und durch diese Vektoren transformierte Wirte.
NZ221627A (en) * 1986-09-09 1993-04-28 Genencor Inc Preparation of enzymes, modifications, catalytic triads to alter ratios or transesterification/hydrolysis ratios
ES2135386T3 (es) * 1987-02-27 1999-11-01 Genencor Int Transformacion de cepas de bacillus alcalofilas.
WO1988008028A1 (en) * 1987-04-06 1988-10-20 Genex Corporation The engineering of electrostatic interactions at metal ion binding sites for the stabilization of proteins
US4914031A (en) * 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
FR2614467A1 (fr) * 1987-04-21 1988-10-28 Telemecanique Electrique Appareil interrupteur de protection a mecanisme de declenchement simplifie

Also Published As

Publication number Publication date
KR100188532B1 (ko) 1999-06-01
ATE141324T1 (de) 1996-08-15
AU618675B2 (en) 1992-01-02
DK0398539T3 (da) 1996-12-09
KR920701426A (ko) 1992-08-11
DE69028040T2 (de) 1997-02-27
JPH03505976A (ja) 1991-12-26
US5397705A (en) 1995-03-14
EP0398539B1 (en) 1996-08-14
CA2016211A1 (en) 1990-11-17
DE69028040D1 (de) 1996-09-19
HK1006857A1 (en) 1999-03-19
AU5558090A (en) 1990-12-18
CA2016211C (en) 1998-07-21
GR3021010T3 (en) 1996-12-31
WO1990014420A1 (en) 1990-11-29
SG47797A1 (en) 1998-04-17
EP0398539A1 (en) 1990-11-22

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