ATE111480T1 - Verfahren zur rückgewinnung rekombinanter proteine unter verwendung von flockungsmitteln. - Google Patents

Verfahren zur rückgewinnung rekombinanter proteine unter verwendung von flockungsmitteln.

Info

Publication number
ATE111480T1
ATE111480T1 AT90912171T AT90912171T ATE111480T1 AT E111480 T1 ATE111480 T1 AT E111480T1 AT 90912171 T AT90912171 T AT 90912171T AT 90912171 T AT90912171 T AT 90912171T AT E111480 T1 ATE111480 T1 AT E111480T1
Authority
AT
Austria
Prior art keywords
protein
molecular weight
high molecular
flocculing
agents
Prior art date
Application number
AT90912171T
Other languages
English (en)
Inventor
Vikram P Mehrotra
Original Assignee
Mallinckrodt Veterinary Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mallinckrodt Veterinary Inc filed Critical Mallinckrodt Veterinary Inc
Application granted granted Critical
Publication of ATE111480T1 publication Critical patent/ATE111480T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Separation Of Suspended Particles By Flocculating Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT90912171T 1989-08-28 1990-07-30 Verfahren zur rückgewinnung rekombinanter proteine unter verwendung von flockungsmitteln. ATE111480T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/399,021 US5047511A (en) 1989-08-28 1989-08-28 Method for recovering recombinant proteins

Publications (1)

Publication Number Publication Date
ATE111480T1 true ATE111480T1 (de) 1994-09-15

Family

ID=23577800

Family Applications (1)

Application Number Title Priority Date Filing Date
AT90912171T ATE111480T1 (de) 1989-08-28 1990-07-30 Verfahren zur rückgewinnung rekombinanter proteine unter verwendung von flockungsmitteln.

Country Status (7)

Country Link
US (1) US5047511A (de)
EP (1) EP0494873B1 (de)
AT (1) ATE111480T1 (de)
AU (1) AU643456B2 (de)
DE (1) DE69012579T2 (de)
NZ (1) NZ235040A (de)
WO (1) WO1991003486A1 (de)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ZA94169B (en) * 1993-01-26 1994-09-07 Lilly Co Eli Non-denaturing potency assay for bovine somatotropin
WO1995032222A1 (en) * 1994-05-19 1995-11-30 Monsanto Company Method for purifying somatotropin monomers
KR100501817B1 (ko) * 1998-07-21 2005-07-20 몬산토 테크놀로지 엘엘씨 음이온 폴리머성 응집제들을 사용한 단백질 침전물 현탁액의 청징화 방법
CN101011573A (zh) * 2001-07-23 2007-08-08 纳蒂芒公司 高分子量凝集素的生产
US8123944B2 (en) * 2001-08-28 2012-02-28 Clearvalue Technologies, Inc. Processes of aqueous solids treatment
DE60332358D1 (de) * 2002-09-09 2010-06-10 Hanall Pharmaceutical Co Ltd Protease-resistente modifizierte interferon alpha polypeptide
US7998930B2 (en) 2004-11-04 2011-08-16 Hanall Biopharma Co., Ltd. Modified growth hormones
AT501380B1 (de) * 2005-02-07 2007-11-15 Vitali Dipl Ing Dshemelinski Verfahren zur reinigung der dna/rna von proteinen und zellulären reststoffen im zellenlysat
FR2902799B1 (fr) 2006-06-27 2012-10-26 Millipore Corp Procede et unite de preparation d'un echantillon pour l'analyse microbiologique d'un liquide
US8569464B2 (en) * 2006-12-21 2013-10-29 Emd Millipore Corporation Purification of proteins
US8362217B2 (en) 2006-12-21 2013-01-29 Emd Millipore Corporation Purification of proteins
US8163886B2 (en) * 2006-12-21 2012-04-24 Emd Millipore Corporation Purification of proteins
WO2009151514A1 (en) * 2008-06-11 2009-12-17 Millipore Corporation Stirred tank bioreactor
CN102257122B (zh) 2008-12-16 2015-07-29 Emd密理博公司 搅拌槽反应器及方法
DK2571903T3 (da) 2010-05-17 2019-11-04 Emd Millipore Corp Polymerer, der reagerer på stimuli, til oprensning af biomolekyler

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2175090A (en) * 1938-06-22 1939-10-03 Lederie Lab Inc Purification of antibody compositions
US3252961A (en) * 1961-04-03 1966-05-24 Foremost Dairies Inc Whey process and product
US3657071A (en) * 1968-11-12 1972-04-18 Hans Ulrich Bergmeyer Fractionation of proteins
DE2001902C3 (de) * 1970-01-16 1978-10-12 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren zur Reinigung und Fraktionierung von gelösten aktiven Proteinen
US3749668A (en) * 1971-05-03 1973-07-31 Rohm & Haas Water purification with amine oxide resins
CH619433A5 (de) * 1976-07-02 1980-09-30 Ciba Geigy Ag
US4055469A (en) * 1976-12-10 1977-10-25 Eastman Kodak Company Purification of microbial enzyme extracts using synthetic polyelectrolytes
DE2718325C2 (de) * 1977-04-25 1986-09-25 Behringwerke Ag, 3550 Marburg Leucinreiches 3,1-S-α↓2↓-Glykoprotein sowie dessen Verwendung
US4204989A (en) * 1978-12-13 1980-05-27 Merck & Co., Inc. Isolation of hepatitis B e antigen
DE2946458A1 (de) * 1979-11-17 1981-06-11 Behringwerke Ag, 3550 Marburg Neues protein pp(pfeil abwaerts)11(pfeil abwaerts)
JPH0738880B2 (ja) * 1983-06-24 1995-05-01 東レ株式会社 エンドトキシン血症治療剤
US4634673A (en) * 1984-03-28 1987-01-06 Nabisco Brands, Inc. Enzyme purification process
JPS60248180A (ja) * 1984-05-24 1985-12-07 Showa Denko Kk カリジノゲナ−ゼの精製方法およびその装置
WO1986001744A1 (fr) * 1984-09-10 1986-03-27 Toray Industries, Inc. Procede d'echange ou d'adsorption d'ions
US4645829A (en) * 1984-10-29 1987-02-24 Monsanto Company Method for separating polypeptides
DK111185A (da) * 1985-03-12 1986-09-13 Novo Industri As Xyloseisomerase, fremgangsmaade til fremstilling deraf, immobiliseret xyloseisomerase og fremgangsmaade til isomerisering af glucose til fructose
SE462100B (sv) * 1985-08-08 1990-05-07 Perstorp Ab Komposition och dess anvaendning i ett tvaa- eller flerfassystem
US4677196A (en) * 1985-09-06 1987-06-30 International Minerals & Chemical Corp. Purification and activation of proteins from insoluble inclusion bodies
DE3608091A1 (de) * 1986-03-12 1987-09-17 Basf Ag Verfahren zum isolieren und reinigen von haemin
US4771128A (en) * 1986-10-10 1988-09-13 Cetus Corporation Method of purifying toxin conjugates using hydrophobic interaction chromatography
US5101018A (en) * 1989-06-12 1992-03-31 International Minerals & Chemical Corp. Method for recovering recombinant proteins

Also Published As

Publication number Publication date
AU643456B2 (en) 1993-11-18
AU6171590A (en) 1991-04-08
DE69012579T2 (de) 1995-02-23
DE69012579D1 (de) 1994-10-20
WO1991003486A1 (en) 1991-03-21
US5047511A (en) 1991-09-10
EP0494873A1 (de) 1992-07-22
NZ235040A (en) 1992-03-26
EP0494873B1 (de) 1994-09-14

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