ATE112315T1 - Hybriden von bacillus thuringiensis, plasmide und transformierte pseudomonas fluorescens. - Google Patents

Hybriden von bacillus thuringiensis, plasmide und transformierte pseudomonas fluorescens.

Info

Publication number
ATE112315T1
ATE112315T1 AT89302049T AT89302049T ATE112315T1 AT E112315 T1 ATE112315 T1 AT E112315T1 AT 89302049 T AT89302049 T AT 89302049T AT 89302049 T AT89302049 T AT 89302049T AT E112315 T1 ATE112315 T1 AT E112315T1
Authority
AT
Austria
Prior art keywords
bacillus thuringiensis
hybrids
plasmids
pseudomonas fluorescens
thuringiensis toxin
Prior art date
Application number
AT89302049T
Other languages
English (en)
Inventor
Thomas E Gilroy
Original Assignee
Mycogen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mycogen Corp filed Critical Mycogen Corp
Application granted granted Critical
Publication of ATE112315T1 publication Critical patent/ATE112315T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/78—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
AT89302049T 1988-03-03 1989-03-01 Hybriden von bacillus thuringiensis, plasmide und transformierte pseudomonas fluorescens. ATE112315T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/164,162 US5055294A (en) 1988-03-03 1988-03-03 Chimeric bacillus thuringiensis crystal protein gene comprising hd-73 and berliner 1715 toxin genes, transformed and expressed in pseudomonas fluorescens

Publications (1)

Publication Number Publication Date
ATE112315T1 true ATE112315T1 (de) 1994-10-15

Family

ID=22593256

Family Applications (1)

Application Number Title Priority Date Filing Date
AT89302049T ATE112315T1 (de) 1988-03-03 1989-03-01 Hybriden von bacillus thuringiensis, plasmide und transformierte pseudomonas fluorescens.

Country Status (7)

Country Link
US (1) US5055294A (de)
EP (1) EP0331470B1 (de)
JP (1) JP3298103B2 (de)
AT (1) ATE112315T1 (de)
CA (1) CA1341153C (de)
DE (1) DE68918477T2 (de)
ES (1) ES2060753T3 (de)

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CA2012025C (en) * 1989-03-14 1999-09-07 Yukiko Tsukuda Dna for expression and secretion
US5593881A (en) * 1994-05-06 1997-01-14 Mycogen Corporation Bacillus thuringiensis delta-endotoxin
US5527883A (en) * 1994-05-06 1996-06-18 Mycogen Corporation Delta-endotoxin expression in pseudomonas fluorescens
US5702703A (en) * 1994-11-16 1997-12-30 Mycogen Corporation Bacillus thuringiensis toxin enhancer
US5508264A (en) * 1994-12-06 1996-04-16 Mycogen Corporation Pesticidal compositions
WO1997013402A1 (en) * 1995-10-13 1997-04-17 Dow Agrosciences Llc Modified bacillus thuringiensis gene for lepidopteran control in plants
US5965428A (en) * 1996-10-08 1999-10-12 Ecogen, Inc. Chimeric lepidopteran-toxic crystal proteins
US6017534A (en) * 1996-11-20 2000-01-25 Ecogen, Inc. Hybrid Bacillus thuringiensis δ-endotoxins with novel broad-spectrum insecticidal activity
JP4389075B2 (ja) 1996-11-20 2009-12-24 モンサント・テクノロジー・エルエルシー 広域スペクトルのデルタ−エンドトキシン
US6713063B1 (en) * 1996-11-20 2004-03-30 Monsanto Technology, Llc Broad-spectrum δ-endotoxins
KR100736253B1 (ko) 1999-03-30 2007-07-06 아그라퀘스트 인코퍼레이티드 식물 질병을 방제하는 ｂａｃｉｌｌｕｓ ｐｕｍｉｌｕｓ의균주
US6245551B1 (en) 1999-03-30 2001-06-12 Agraquest, Inc. Strain of Bacillus pumilus for controlling plant diseases caused by fungi
AU2002306484A1 (en) 2002-02-13 2003-09-04 Dow Global Technologies Inc. Over-expression of extremozyme genes in pseudomonads and closely related bacteria
US7053266B2 (en) * 2002-03-27 2006-05-30 Council Of Scientfic And Industrial Research Chimeric cry1E δendotoxin and methods of controlling insects
US7557186B2 (en) * 2002-03-27 2009-07-07 Council Of Scientific & Industrial Research Chimeric CrylE δ endotoxin and methods of controlling insects
CA2482995C (en) * 2002-04-22 2013-01-29 Dow Global Technologies Inc. Low-cost production of peptides
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
EP1692282B1 (de) 2003-11-19 2012-08-08 Pfenex, Inc. Verbesserte Proteinexpressionssysteme in auxotrophem Pseudomonas fluorescens
BRPI0416807A (pt) 2003-11-21 2007-01-09 Dow Global Technologies Inc sistemas de expressão aperfeiçoados com secreção de sistema sec
CA2547511A1 (en) * 2003-12-01 2005-07-28 Dow Global Technolgies Inc. Recombinant icosahedral virus like particle production in pseudomonads
KR101183720B1 (ko) 2004-01-16 2012-09-17 다우 글로벌 테크놀로지스 엘엘씨 슈도모나스 플루오레센스에서의 포유류 단백질의 발현
CN101031655A (zh) 2004-07-26 2007-09-05 陶氏环球技术公司 通过株工程改进蛋白表达的方法
PT1919935E (pt) 2005-08-31 2013-03-14 Monsanto Technology Llc Sequências de nucleótidos que codificam proteínas insecticidas
EP2021476B1 (de) 2006-05-26 2014-07-09 Monsanto Technology, LLC Maispflanze und samen entsprechend dem transgenen ereignis mon89034 und verfahren zu ihrem nachweis und ihrer verwendung
KR101491867B1 (ko) 2007-01-31 2015-02-10 피페넥스 인크. 증가된 발현을 위한 박테리아 리더 서열
US9394571B2 (en) 2007-04-27 2016-07-19 Pfenex Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
US20110020830A1 (en) * 2008-03-31 2011-01-27 Schneider Jane C Design for rapidly cloning one or more polypeptide chains into an expression system
AU2010247938B2 (en) 2009-05-11 2016-02-18 Pelican Technology Holdings, Inc. Production of recombinant proteins utilizing non-antibiotic selection methods and the incorporation of non-natural amino acids therein
MX2012010148A (es) 2010-03-04 2013-01-22 Pfenex Inc Metodo para producir proteinas de interferon recombinantes solubles sin desnaturalizacion.
WO2011126811A2 (en) 2010-03-30 2011-10-13 Pfenex Inc. High level expression of recombinant toxin proteins
PL2552949T3 (pl) 2010-04-01 2017-01-31 Pfenex Inc. Sposoby wytwarzania G-CSF w komórce gospodarza Pseudomonas
CN112400900A (zh) 2012-03-09 2021-02-26 韦斯塔隆公司 毒肽产生、植物中的肽表达和富含半胱氨酸的肽的组合
US11692016B2 (en) 2012-03-09 2023-07-04 Vestaron Corporation High gene expression yeast strain
US9169304B2 (en) 2012-05-01 2015-10-27 Pfenex Inc. Process for purifying recombinant Plasmodium falciparum circumsporozoite protein
MX388191B (es) 2014-11-20 2025-03-19 Biological E Ltd Polinucleótido optimizado por codones para la expresión de alto nivel de crm197.
WO2016089782A1 (en) 2014-12-01 2016-06-09 Pfenex Inc. Fusion partners for peptide production
EP3240560B1 (de) 2014-12-30 2022-08-31 Corteva Agriscience LLC Modifizierte cry1ca-toxine zur bekämpfung von insektenschädlingen
US10676723B2 (en) 2015-05-11 2020-06-09 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
US11535653B2 (en) 2016-10-21 2022-12-27 Vestaron Corporation Cleavable peptides and insecticidal and nematicidal proteins comprising same
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
EP3701036A4 (de) 2017-10-27 2021-09-01 Pfenex Inc. Verfahren zur herstellung von rekombinantem e. coli
KR102835683B1 (ko) 2017-10-27 2025-07-17 펠리칸 테크놀로지 홀딩스, 인크. 주변세포질 단백질 발현을 위한 박테리아 리더 서열
SG11202002943PA (en) 2017-10-27 2020-05-28 Pfenex Inc Method for production of recombinant erwinia asparaginase
WO2021216621A2 (en) 2020-04-20 2021-10-28 Vestaron Corporation Proteolytically stable u1-agatoxin-ta1b variant polypeptides for pest control
KR20230125179A (ko) 2020-12-23 2023-08-29 재즈 파마슈티칼즈 아일랜드 리미티드 전하-차폐 융합 단백질의 정제 방법
WO2022211829A1 (en) 2021-03-30 2022-10-06 Jazz Pharmaceuticals Ireland Ltd. Dosing of recombinant l-asparaginase
KR20240021234A (ko) 2021-06-10 2024-02-16 펠리칸 테크놀로지 홀딩스, 인크. 재조합 단백질 발현을 위한 박테리아 숙주

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4448885A (en) * 1981-04-27 1984-05-15 Board Of The Regents Of The University Of Washington Bacillus thuringiensis crystal protein in Escherichia coli
US4467036A (en) * 1981-11-12 1984-08-21 The Board Of Regents Of The University Of Washington Bacillus thuringiensis crystal protein in Escherichia coli
EP0200708B1 (de) * 1985-04-30 1992-12-30 Monsanto Company Pflanzenkolonisierende Mikroorganismen, die das Bacillus thuringiensis-Toxingen als Chromosomeinsetzung enthalten
US4771131A (en) * 1985-08-16 1988-09-13 Mycogen Corporation Cloning and expression of Bacillus thuringiensis toxin gene encoding a protein toxic to beetles of the order Coleoptera
CA1341092C (en) * 1985-12-12 2000-09-05 David L. Edwards Process for altering the host range of bacillus thuringiensis toxins, and novel toxins produced thereby

Also Published As

Publication number Publication date
EP0331470B1 (de) 1994-09-28
JPH025873A (ja) 1990-01-10
US5055294A (en) 1991-10-08
CA1341153C (en) 2000-12-12
JP3298103B2 (ja) 2002-07-02
ES2060753T3 (es) 1994-12-01
DE68918477D1 (de) 1994-11-03
EP0331470A2 (de) 1989-09-06
EP0331470A3 (de) 1991-03-06
DE68918477T2 (de) 1995-02-16

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