ATE112315T1 - Hybriden von bacillus thuringiensis, plasmide und transformierte pseudomonas fluorescens. - Google Patents

Hybriden von bacillus thuringiensis, plasmide und transformierte pseudomonas fluorescens.

Info

Publication number
ATE112315T1
ATE112315T1 AT89302049T AT89302049T ATE112315T1 AT E112315 T1 ATE112315 T1 AT E112315T1 AT 89302049 T AT89302049 T AT 89302049T AT 89302049 T AT89302049 T AT 89302049T AT E112315 T1 ATE112315 T1 AT E112315T1
Authority
AT
Austria
Prior art keywords
bacillus thuringiensis
hybrids
plasmids
pseudomonas fluorescens
thuringiensis toxin
Prior art date
Application number
AT89302049T
Other languages
English (en)
Inventor
Thomas E Gilroy
Original Assignee
Mycogen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mycogen Corp filed Critical Mycogen Corp
Application granted granted Critical
Publication of ATE112315T1 publication Critical patent/ATE112315T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/78—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
AT89302049T 1988-03-03 1989-03-01 Hybriden von bacillus thuringiensis, plasmide und transformierte pseudomonas fluorescens. ATE112315T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/164,162 US5055294A (en) 1988-03-03 1988-03-03 Chimeric bacillus thuringiensis crystal protein gene comprising hd-73 and berliner 1715 toxin genes, transformed and expressed in pseudomonas fluorescens

Publications (1)

Publication Number Publication Date
ATE112315T1 true ATE112315T1 (de) 1994-10-15

Family

ID=22593256

Family Applications (1)

Application Number Title Priority Date Filing Date
AT89302049T ATE112315T1 (de) 1988-03-03 1989-03-01 Hybriden von bacillus thuringiensis, plasmide und transformierte pseudomonas fluorescens.

Country Status (7)

Country Link
US (1) US5055294A (de)
EP (1) EP0331470B1 (de)
JP (1) JP3298103B2 (de)
AT (1) ATE112315T1 (de)
CA (1) CA1341153C (de)
DE (1) DE68918477T2 (de)
ES (1) ES2060753T3 (de)

Families Citing this family (46)

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CA2012025C (en) * 1989-03-14 1999-09-07 Yukiko Tsukuda Dna for expression and secretion
US5527883A (en) * 1994-05-06 1996-06-18 Mycogen Corporation Delta-endotoxin expression in pseudomonas fluorescens
US5593881A (en) * 1994-05-06 1997-01-14 Mycogen Corporation Bacillus thuringiensis delta-endotoxin
US5702703A (en) * 1994-11-16 1997-12-30 Mycogen Corporation Bacillus thuringiensis toxin enhancer
US5508264A (en) * 1994-12-06 1996-04-16 Mycogen Corporation Pesticidal compositions
EP0861021B1 (de) * 1995-10-13 2009-09-23 Dow Agrosciences LLC Modifiziertes bacillus thuringiensis gen zur kontrolle von lepidoptera in pflanzen
US5965428A (en) * 1996-10-08 1999-10-12 Ecogen, Inc. Chimeric lepidopteran-toxic crystal proteins
US6017534A (en) 1996-11-20 2000-01-25 Ecogen, Inc. Hybrid Bacillus thuringiensis δ-endotoxins with novel broad-spectrum insecticidal activity
US6713063B1 (en) 1996-11-20 2004-03-30 Monsanto Technology, Llc Broad-spectrum δ-endotoxins
AP9901541A0 (en) * 1996-11-20 1999-06-30 Ecogen Inc Broad-spectrum delta-endotoxins.
HUP0200562A2 (en) 1999-03-30 2002-06-29 Agraquest Inc A strain of baccilus pumilus for controlling plant diseases
US6245551B1 (en) 1999-03-30 2001-06-12 Agraquest, Inc. Strain of Bacillus pumilus for controlling plant diseases caused by fungi
AU2002306484A1 (en) 2002-02-13 2003-09-04 Dow Global Technologies Inc. Over-expression of extremozyme genes in pseudomonads and closely related bacteria
US7053266B2 (en) * 2002-03-27 2006-05-30 Council Of Scientfic And Industrial Research Chimeric cry1E δendotoxin and methods of controlling insects
US7557186B2 (en) 2002-03-27 2009-07-07 Council Of Scientific & Industrial Research Chimeric CrylE δ endotoxin and methods of controlling insects
CA2482995C (en) * 2002-04-22 2013-01-29 Dow Global Technologies Inc. Low-cost production of peptides
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
WO2005052151A1 (en) 2003-11-19 2005-06-09 Dow Global Technologies Inc. Improved protein expression systems
CA2546157C (en) 2003-11-21 2014-07-22 Dow Global Technolgies Inc. Improved expression systems with sec-system secretion
CA2547511A1 (en) * 2003-12-01 2005-07-28 Dow Global Technolgies Inc. Recombinant icosahedral virus like particle production in pseudomonads
EP1709170B1 (de) 2004-01-16 2018-02-21 Pfenex Inc. Expression von säugerproteinen in pseudomonas fluorescens
BRPI0513826A2 (pt) 2004-07-26 2010-06-22 Dow Global Technologies Inc processo para expressão de proteìna melhorada através de engenharia de cepa
EA015908B1 (ru) * 2005-08-31 2011-12-30 Монсанто Текнолоджи, Ллс Инсектицидный белок в.thuringiensis cry1a.105, кодирующий его полинуклеотид и их применение
EP2021476B1 (de) 2006-05-26 2014-07-09 Monsanto Technology, LLC Maispflanze und samen entsprechend dem transgenen ereignis mon89034 und verfahren zu ihrem nachweis und ihrer verwendung
EP2468869B1 (de) 2007-01-31 2015-03-18 Pfenex Inc. Bakterielle Leader-Sequenzen für erhöhte Expression
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
US9394571B2 (en) 2007-04-27 2016-07-19 Pfenex Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
US20110020830A1 (en) * 2008-03-31 2011-01-27 Schneider Jane C Design for rapidly cloning one or more polypeptide chains into an expression system
AU2010247938B2 (en) 2009-05-11 2016-02-18 Pelican Technology Holdings, Inc. Production of recombinant proteins utilizing non-antibiotic selection methods and the incorporation of non-natural amino acids therein
EP2542574B1 (de) * 2010-03-04 2017-08-09 Pfenex Inc. Verfahren zur herstellung eines löslichen und rekombinanten interferonproteins ohne denaturierung
KR20130072201A (ko) * 2010-03-30 2013-07-01 피페넥스 인크. 재조합 독소 단백질의 고수준 발현
AU2011235210B2 (en) 2010-04-01 2015-07-16 Pfenex Inc. Methods for G-CSF production in a Pseudomonas host cell
NZ727213A (en) 2012-03-09 2020-03-27 Vestaron Corp Toxic peptide production, peptide expression in plants and combinations of cysteine rich peptides
US11692016B2 (en) 2012-03-09 2023-07-04 Vestaron Corporation High gene expression yeast strain
US9169304B2 (en) 2012-05-01 2015-10-27 Pfenex Inc. Process for purifying recombinant Plasmodium falciparum circumsporozoite protein
CA2968491C (en) 2014-11-20 2023-09-26 Biological E Limited Codon optimized polynucleotide for high level expression of crm197
CN107532190B (zh) 2014-12-01 2021-07-09 菲尼克斯公司 用于肽生产的融合伴侣
CA3192494A1 (en) 2014-12-30 2016-07-07 Corteva Agriscience Llc Modified cry1ca toxins useful for control of insect pests
US10676723B2 (en) 2015-05-11 2020-06-09 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
BR112019008023A2 (pt) 2016-10-21 2019-07-09 Vestaron Corp peptídeo, proteína inseticida e/ou nematicida, polinucleotídeo, vetor, célula hospedeira, constructo de dna, planta, ou parte da mesma, e, método de controle de uma infecção por peste de uma planta.
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
CA3077393A1 (en) 2017-10-27 2019-05-02 Pfenex Inc. Bacterial leader sequences for periplasmic protein expression
EP3701036A4 (de) 2017-10-27 2021-09-01 Pfenex Inc. Verfahren zur herstellung von rekombinantem e. coli
JP7784804B2 (ja) 2017-10-27 2025-12-12 フェネックス インク． 組み換えｅｒｗｉｎｉａアスパラギナーゼの製造のための方法
AU2021410080A1 (en) 2020-12-23 2023-06-22 Jazz Pharmaceuticals Ireland Ltd. Methods of purifying charge-shielded fusion proteins
WO2022211829A1 (en) 2021-03-30 2022-10-06 Jazz Pharmaceuticals Ireland Ltd. Dosing of recombinant l-asparaginase

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4467036A (en) * 1981-11-12 1984-08-21 The Board Of Regents Of The University Of Washington Bacillus thuringiensis crystal protein in Escherichia coli
US4448885A (en) * 1981-04-27 1984-05-15 Board Of The Regents Of The University Of Washington Bacillus thuringiensis crystal protein in Escherichia coli
AU590558B2 (en) * 1985-04-30 1989-11-09 Monsanto Company Plant-colonizing microorganisms containing the toxin gene of b. thuringiensis as a chromosomal insertion
US4771131A (en) * 1985-08-16 1988-09-13 Mycogen Corporation Cloning and expression of Bacillus thuringiensis toxin gene encoding a protein toxic to beetles of the order Coleoptera
CA1341092C (en) * 1985-12-12 2000-09-05 David L. Edwards Process for altering the host range of bacillus thuringiensis toxins, and novel toxins produced thereby

Also Published As

Publication number Publication date
ES2060753T3 (es) 1994-12-01
JP3298103B2 (ja) 2002-07-02
DE68918477T2 (de) 1995-02-16
EP0331470B1 (de) 1994-09-28
EP0331470A3 (de) 1991-03-06
US5055294A (en) 1991-10-08
CA1341153C (en) 2000-12-12
EP0331470A2 (de) 1989-09-06
JPH025873A (ja) 1990-01-10
DE68918477D1 (de) 1994-11-03

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