BRPI0416807A - sistemas de expressão aperfeiçoados com secreção de sistema sec - Google Patents

sistemas de expressão aperfeiçoados com secreção de sistema sec

Info

Publication number
BRPI0416807A
BRPI0416807A BRPI0416807-0A BRPI0416807A BRPI0416807A BR PI0416807 A BRPI0416807 A BR PI0416807A BR PI0416807 A BRPI0416807 A BR PI0416807A BR PI0416807 A BRPI0416807 A BR PI0416807A
Authority
BR
Brazil
Prior art keywords
sec
secretion
expression systems
systems
sec system
Prior art date
Application number
BRPI0416807-0A
Other languages
English (en)
Inventor
Diane M Retallack
Jane C Schneider
Thomas Martin Ramseier
Original Assignee
Dow Global Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow Global Technologies Inc filed Critical Dow Global Technologies Inc
Publication of BRPI0416807A publication Critical patent/BRPI0416807A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/21—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Pseudomonadaceae (F)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/16—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/78—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Pseudomonas
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Endocrinology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Botany (AREA)
  • Toxicology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

"SISTEMAS DE EXPRESSãO APERFEIçOADOS COM SECREçãO DE SISTEMA SEC". Métodos e sistemas de expressão procarióticos aperfeiçoados para produção de proteínas recombinantes usando peptídeos de sinal de secreção de sistema Sec são descritos usando sistemas de secreção de Sec de Pseudomonas fluorescens. Peptídeos de sinal de secreção de sistema Sec novos específicos são descritos, como o são proteínas de fusão e seqüências de codificação para secreção aperfeiçoada de proteínas e peptídeos recombinantes.
BRPI0416807-0A 2003-11-21 2004-11-22 sistemas de expressão aperfeiçoados com secreção de sistema sec BRPI0416807A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US52412403P 2003-11-21 2003-11-21
PCT/US2004/039316 WO2005089093A2 (en) 2003-11-21 2004-11-22 Improved expression systems with sec-system secretion

Publications (1)

Publication Number Publication Date
BRPI0416807A true BRPI0416807A (pt) 2007-01-09

Family

ID=34994140

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0416807-0A BRPI0416807A (pt) 2003-11-21 2004-11-22 sistemas de expressão aperfeiçoados com secreção de sistema sec

Country Status (11)

Country Link
US (1) US7985564B2 (pt)
EP (4) EP1687324B1 (pt)
JP (1) JP5028551B2 (pt)
KR (1) KR101265343B1 (pt)
CN (1) CN1882605B (pt)
AU (1) AU2004317306B2 (pt)
BR (1) BRPI0416807A (pt)
CA (1) CA2546157C (pt)
MX (1) MXPA06005705A (pt)
PL (2) PL2336153T3 (pt)
WO (1) WO2005089093A2 (pt)

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9453251B2 (en) 2002-10-08 2016-09-27 Pfenex Inc. Expression of mammalian proteins in Pseudomonas fluorescens
CA2546157C (en) 2003-11-21 2014-07-22 Dow Global Technolgies Inc. Improved expression systems with sec-system secretion
BRPI0513826A2 (pt) 2004-07-26 2010-06-22 Dow Global Technologies Inc processo para expressão de proteìna melhorada através de engenharia de cepa
JP5130662B2 (ja) * 2006-06-07 2013-01-30 Ｊｎｃ株式会社 タンパク質の可溶性タンパク質としての製造方法
EP2468869B1 (en) 2007-01-31 2015-03-18 Pfenex Inc. Bacterial leader sequences for increased expression
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
US9394571B2 (en) 2007-04-27 2016-07-19 Pfenex Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
US8318481B2 (en) 2007-12-07 2012-11-27 Pfenex Inc. High copy number self-replicating plasmids in pseudomonas
WO2010008764A1 (en) * 2008-06-23 2010-01-21 Dow Global Technologies Inc. Pseudomonas fluorescens strains for production of extracellular recombinant protein
PL2419441T3 (pl) 2009-04-17 2015-06-30 Dow Agrosciences Llc Owadzie toksyny Cry obejmujące DIG-3
BR112012014801B1 (pt) 2009-12-16 2024-03-05 Dow Agrosciences Llc Métodos para controlar o desenvolvimento da resistência de um inseto à uma proteína inseticida derivada de um bacillus thuringiensis, e para o controle de pragas de lepidópteros, bem como composição para o controle de pragas de lepidópteros
EP2542574B1 (en) * 2010-03-04 2017-08-09 Pfenex Inc. Method for producing soluble recombinant interferon protein without denaturing
KR20130072201A (ko) 2010-03-30 2013-07-01 피페넥스 인크. 재조합 독소 단백질의 고수준 발현
AU2011235210B2 (en) 2010-04-01 2015-07-16 Pfenex Inc. Methods for G-CSF production in a Pseudomonas host cell
NZ603561A (en) 2010-04-23 2015-06-26 Dow Agrosciences Llc Combinations including cry34ab/35ab and cry3aa proteins to prevent development of resistance in corn rootworms (diabrotica spp.)
US9234208B1 (en) 2010-05-10 2016-01-12 Dow Agrosciences Llc DIG-13 insecticidal cry toxins
AR084293A1 (es) 2010-12-16 2013-05-08 Dow Agrosciences Llc Cry1FA RADIOMARCADA, BIOLOGICAMENTE ACTIVA Y METODOS DE ENSAYOS DE UNION AL RECEPTOR
ES3011307T3 (en) 2012-02-23 2025-04-07 Juno Therapeutics Gmbh Chromatographic isolation of cells and other complex biological materials
WO2013163654A2 (en) * 2012-04-27 2013-10-31 Pronutria, Inc. Nucleic acids, cells, and methods for producing secreted proteins
US9169304B2 (en) 2012-05-01 2015-10-27 Pfenex Inc. Process for purifying recombinant Plasmodium falciparum circumsporozoite protein
CN103254277B (zh) * 2013-05-03 2017-02-08 南京工业大学 强分泌性信号肽增强小肽模序及其应用
TW201542093A (zh) 2014-03-21 2015-11-16 艾格里遺傳學股份有限公司 用於控制玉米穗蟲之Ｃｒｙ１Ｄ
JP2017521055A (ja) 2014-06-20 2017-08-03 ダウ アグロサイエンシィズ エルエルシー 害虫防除に有用な植物性殺虫タンパク質
CN107532190B (zh) 2014-12-01 2021-07-09 菲尼克斯公司 用于肽生产的融合伴侣
CA3192494A1 (en) 2014-12-30 2016-07-07 Corteva Agriscience Llc Modified cry1ca toxins useful for control of insect pests
MX2018004875A (es) 2015-10-22 2018-08-01 Juno Therapeutics Gmbh Metodos para cultivar celulas y kits y aparatos para ello.
EP3365453A2 (en) 2015-10-22 2018-08-29 Juno Therapeutics GmbH Methods, kits, agents and apparatuses for transduction
WO2018063870A1 (en) 2016-09-30 2018-04-05 Dow Agrosciences Llc Binary insecticidal cry toxins
JP7339160B2 (ja) 2017-04-27 2023-09-05 ジュノ セラピューティクス ゲーエムベーハー オリゴマー粒子試薬およびその使用方法
EP3701036A4 (en) 2017-10-27 2021-09-01 Pfenex Inc. PROCESS FOR PRODUCING ASPARAGINASE OF E. COLI
CA3077393A1 (en) 2017-10-27 2019-05-02 Pfenex Inc. Bacterial leader sequences for periplasmic protein expression
JP7784804B2 (ja) 2017-10-27 2025-12-12 フェネックス インク． 組み換えｅｒｗｉｎｉａアスパラギナーゼの製造のための方法
CN113667685B (zh) * 2018-08-07 2023-02-28 康码（上海）生物科技有限公司 信号肽相关序列及其在蛋白质合成中的应用
SG11202104355SA (en) 2018-10-31 2021-05-28 Juno Therapeutics Gmbh Methods for selection and stimulation of cells and apparatus for same
CN112812176B (zh) * 2021-01-13 2022-10-11 江南大学 一种通过低盐絮凝法从螺旋藻中提取藻蓝蛋白的方法
WO2022211829A1 (en) 2021-03-30 2022-10-06 Jazz Pharmaceuticals Ireland Ltd. Dosing of recombinant l-asparaginase

Family Cites Families (64)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4551433A (en) 1981-05-18 1985-11-05 Genentech, Inc. Microbial hybrid promoters
US4595658A (en) 1982-09-13 1986-06-17 The Rockefeller University Method for facilitating externalization of proteins synthesized in bacteria
US4511503A (en) 1982-12-22 1985-04-16 Genentech, Inc. Purification and activity assurance of precipitated heterologous proteins
US4513600A (en) 1983-01-03 1985-04-30 The Minster Machine Company Cam actuated ejector for a shell press
GB8308483D0 (en) 1983-03-28 1983-05-05 Health Lab Service Board Secretion of gene products
US4755465A (en) 1983-04-25 1988-07-05 Genentech, Inc. Secretion of correctly processed human growth hormone in E. coli and Pseudomonas
US4680264A (en) 1983-07-01 1987-07-14 Lubrizol Genetics, Inc. Class II mobilizable gram-negative plasmid
US5281532A (en) 1983-07-27 1994-01-25 Mycogen Corporation Pseudomas hosts transformed with bacillus endotoxin genes
US4637980A (en) 1983-08-09 1987-01-20 Smithkline Beckman Corporation Externalization of products of bacteria
US5288852A (en) * 1984-03-06 1994-02-22 Dainippon Pharmaceutical Co., Ltd. Human tumor necrosis factor polypeptides
EP0155189A3 (en) 1984-03-16 1987-09-16 Genentech, Inc. Expression of cell wall degrading proteins and host cells harboring dna encoding such protein
US4963495A (en) 1984-10-05 1990-10-16 Genentech, Inc. Secretion of heterologous proteins
EP0177343B1 (en) 1984-10-05 1992-07-22 Genentech, Inc. Dna, cell cultures and methods for the secretion of heterologous proteins and periplasmic protein recovery
US4695462A (en) 1985-06-28 1987-09-22 Mycogen Corporation Cellular encapsulation of biological pesticides
US4695455A (en) 1985-01-22 1987-09-22 Mycogen Corporation Cellular encapsulation of pesticides produced by expression of heterologous genes
GB8517071D0 (en) 1985-07-05 1985-08-14 Hoffmann La Roche Gram-positive expression control sequence
US5618920A (en) 1985-11-01 1997-04-08 Xoma Corporation Modular assembly of antibody genes, antibodies prepared thereby and use
GB8529014D0 (en) 1985-11-25 1986-01-02 Biogen Nv Enhanced secretion of heterologous proteins
US5232840A (en) * 1986-03-27 1993-08-03 Monsanto Company Enhanced protein production in bacteria by employing a novel ribosome binding site
US5084384A (en) * 1987-04-23 1992-01-28 Monsanto Company Secretion of insulin-like growth factor-I
EP0288451B1 (en) 1987-04-23 1994-08-10 Monsanto Company Secretion of insulin-like growth factor 1 in E. coli
US5128130A (en) 1988-01-22 1992-07-07 Mycogen Corporation Hybrid Bacillus thuringiensis gene, plasmid and transformed Pseudomonas fluorescens
US5055294A (en) 1988-03-03 1991-10-08 Mycogen Corporation Chimeric bacillus thuringiensis crystal protein gene comprising hd-73 and berliner 1715 toxin genes, transformed and expressed in pseudomonas fluorescens
EP0422049A4 (en) 1988-05-09 1991-11-13 Us Health Vector for secretion of proteins directly into periplasm or culture medium
US5169772A (en) 1988-06-06 1992-12-08 Massachusetts Institute Of Technology Large scale method for purification of high purity heparinase from flavobacterium heparinum
US6780613B1 (en) 1988-10-28 2004-08-24 Genentech, Inc. Growth hormone variants
US5175099A (en) * 1989-05-17 1992-12-29 Research Corporation Technologies, Inc. Retrovirus-mediated secretion of recombinant products
US5169760A (en) 1989-07-27 1992-12-08 Mycogen Corporation Method, vectors, and host cells for the control of expression of heterologous genes from lac operated promoters
US5641671A (en) 1990-07-06 1997-06-24 Unilever Patent Holdings B.V. Production of active Pseudomonas glumae lipase in homologous or heterologous hosts
FI913434A7 (fi) 1990-07-16 1992-01-17 Technical Research Centre F Finland Rekombinanta fusionsproteiner vilka utsoendras.
US5417971A (en) * 1991-10-22 1995-05-23 University Of Saskatchewan Vaccines for Actinobacillus pleuropneumoniae
US5348867A (en) * 1991-11-15 1994-09-20 George Georgiou Expression of proteins on bacterial surface
US5563046A (en) * 1993-08-02 1996-10-08 Celtrix Pharmaceuticals, Inc. Fusion polypeptides and proteins
US5914254A (en) 1993-08-02 1999-06-22 Celtrix Pharmaceuticals, Inc. Expression of fusion polypeptides transported out of the cytoplasm without leader sequences
US5527883A (en) 1994-05-06 1996-06-18 Mycogen Corporation Delta-endotoxin expression in pseudomonas fluorescens
EP0797671B1 (en) 1994-12-09 2004-03-03 Novo Nordisk A/S A process of producing extracellular proteins in bacteria
US6495357B1 (en) 1995-07-14 2002-12-17 Novozyme A/S Lipolytic enzymes
IT1283225B1 (it) * 1996-03-11 1998-04-16 Renata Maria Anna Ve Cavaliere Ceppi di batteri, composizione farmaceutica contenente uno o piu' di tali ceppi e uso dei medesimi per la prevenzione e la terapia delle
EP1557467B1 (en) 1996-08-16 2008-05-21 Genencor International, Inc. Expression system derived from the lipase regulation cascade of Pseudomonas alcaligenes
US6361989B1 (en) * 1997-10-13 2002-03-26 Novozymes A/S α-amylase and α-amylase variants
US6551795B1 (en) * 1998-02-18 2003-04-22 Genome Therapeutics Corporation Nucleic acid and amino acid sequences relating to pseudomonas aeruginosa for diagnostics and therapeutics
US6156552A (en) 1998-02-18 2000-12-05 Novo Nordisk A/S Lipase variants
US20030064435A1 (en) 1998-05-28 2003-04-03 Weiner Joel Hirsch Compositions and methods for protein secretion
US6323007B1 (en) 1998-09-18 2001-11-27 Novozymes A/S 2,6-β-D-fructan hydrolase enzyme and processes for using the enzyme
PT1124941E (pt) 1998-10-28 2004-02-27 Genentech Inc Processo para recuperar polipeptidos heterologos a partir de celulas bacterianas
KR100312456B1 (ko) 1999-03-13 2001-11-03 윤덕용 슈도모나스 플루오레슨스 유래의 외래단백질 분비촉진유전자
AU779814B2 (en) 1999-03-29 2005-02-10 Novozymes A/S Polypeptides having branching enzyme activity and nucleic acids encoding same
US6664386B1 (en) 1999-04-08 2003-12-16 The Research Foundation Of State University Of New York System for efficient secretion of recombinant proteins
US6558939B1 (en) 1999-08-31 2003-05-06 Novozymes, A/S Proteases and variants thereof
DE19944870A1 (de) 1999-09-18 2001-03-29 Aventis Pharma Gmbh Signalsequenzen zur Herstellung von Leu-Hirudin über Sekretion durch E. coli in das Kulturmedium
US6617143B1 (en) 1999-10-20 2003-09-09 Novozymes A/S Polypeptides having glucanotransferase activity and nucleic acids encoding same
US6509181B1 (en) * 2000-04-14 2003-01-21 Novozymes, A/S Polypeptides having haloperoxide activity
GB0027782D0 (en) 2000-11-14 2000-12-27 Boehringer Ingelheim Int Methods fro large scale protein productio in prokaryotes
US7087412B2 (en) 2000-11-14 2006-08-08 Boehringer Ingelheim International Gmbh Methods for large scale protein production in prokaryotes
US7202059B2 (en) * 2001-02-20 2007-04-10 Sanofi-Aventis Deutschland Gmbh Fusion proteins capable of being secreted into a fermentation medium
DE10108212A1 (de) 2001-02-20 2002-08-22 Aventis Pharma Gmbh Fusionsprotein zur Sekretion von Wertprotein in bakterielle Überstände
US7419783B2 (en) 2001-11-05 2008-09-02 Research Development Foundation Engineering of leader peptides for the secretion of recombinant proteins in bacteria
AU2002306484A1 (en) 2002-02-13 2003-09-04 Dow Global Technologies Inc. Over-expression of extremozyme genes in pseudomonads and closely related bacteria
AU2003218217A1 (en) 2002-03-15 2003-09-29 The Trustees Of The University Of Pennsylvania Heterologous protein production using the twin arginine translocation pathway
CA2482995C (en) 2002-04-22 2013-01-29 Dow Global Technologies Inc. Low-cost production of peptides
EP1539794B1 (en) 2002-07-03 2008-12-31 Dow Global Technologies Inc. Benzoate- and anthranilate-inducible promoters
CA2546157C (en) 2003-11-21 2014-07-22 Dow Global Technolgies Inc. Improved expression systems with sec-system secretion
EP1709170B1 (en) * 2004-01-16 2018-02-21 Pfenex Inc. Expression of mammalian proteins in pseudomonas fluorescens
EP2468869B1 (en) * 2007-01-31 2015-03-18 Pfenex Inc. Bacterial leader sequences for increased expression

Also Published As

Publication number Publication date
KR101265343B1 (ko) 2013-05-20
EP2327718B1 (en) 2016-03-23
WO2005089093A3 (en) 2006-03-02
EP2336153B1 (en) 2016-03-30
AU2004317306A1 (en) 2005-09-29
EP1687324B1 (en) 2012-08-22
JP5028551B2 (ja) 2012-09-19
CA2546157A1 (en) 2005-09-29
CA2546157C (en) 2014-07-22
PL2327718T3 (pl) 2016-10-31
EP1687324A2 (en) 2006-08-09
CN1882605A (zh) 2006-12-20
CN1882605B (zh) 2012-07-11
EP1687324A4 (en) 2007-08-22
AU2004317306B2 (en) 2010-09-02
JP2007512028A (ja) 2007-05-17
EP2327718A1 (en) 2011-06-01
US7985564B2 (en) 2011-07-26
WO2005089093A2 (en) 2005-09-29
KR20060123173A (ko) 2006-12-01
EP2336153A1 (en) 2011-06-22
EP2314602A1 (en) 2011-04-27
MXPA06005705A (es) 2006-08-23
PL2336153T3 (pl) 2016-08-31
US20060008877A1 (en) 2006-01-12

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