ATE113985T1 - Verfahren zur reinigung von rekombinanten polypeptiden. - Google Patents
Verfahren zur reinigung von rekombinanten polypeptiden.Info
- Publication number
- ATE113985T1 ATE113985T1 AT88903520T AT88903520T ATE113985T1 AT E113985 T1 ATE113985 T1 AT E113985T1 AT 88903520 T AT88903520 T AT 88903520T AT 88903520 T AT88903520 T AT 88903520T AT E113985 T1 ATE113985 T1 AT E113985T1
- Authority
- AT
- Austria
- Prior art keywords
- cleavage site
- purification
- fused
- amino acids
- leader sequence
- Prior art date
Links
- 229920001184 polypeptide Polymers 0.000 title abstract 5
- 102000004196 processed proteins & peptides Human genes 0.000 title abstract 5
- 108090000765 processed proteins & peptides Proteins 0.000 title abstract 5
- 238000000034 method Methods 0.000 title abstract 4
- 238000000746 purification Methods 0.000 title abstract 3
- 238000003776 cleavage reaction Methods 0.000 abstract 5
- 230000007017 scission Effects 0.000 abstract 5
- 150000001413 amino acids Chemical class 0.000 abstract 2
- 102000005593 Endopeptidases Human genes 0.000 abstract 1
- 108010059378 Endopeptidases Proteins 0.000 abstract 1
- 108020004511 Recombinant DNA Proteins 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 238000002955 isolation Methods 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/485—Epidermal growth factor [EGF], i.e. urogastrone
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/585—Calcitonins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Medicinal Chemistry (AREA)
- Toxicology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Endocrinology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US2848487A | 1987-03-20 | 1987-03-20 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE113985T1 true ATE113985T1 (de) | 1994-11-15 |
Family
ID=21843701
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT88903520T ATE113985T1 (de) | 1987-03-20 | 1988-03-04 | Verfahren zur reinigung von rekombinanten polypeptiden. |
Country Status (7)
| Country | Link |
|---|---|
| EP (2) | EP0621340A1 (de) |
| JP (1) | JPH02500876A (de) |
| AT (1) | ATE113985T1 (de) |
| AU (1) | AU605291B2 (de) |
| CA (1) | CA1339208C (de) |
| DE (1) | DE3852074D1 (de) |
| WO (1) | WO1988007085A1 (de) |
Families Citing this family (69)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4946778A (en) * | 1987-09-21 | 1990-08-07 | Genex Corporation | Single polypeptide chain binding molecules |
| US5260203A (en) * | 1986-09-02 | 1993-11-09 | Enzon, Inc. | Single polypeptide chain binding molecules |
| US5869620A (en) * | 1986-09-02 | 1999-02-09 | Enzon, Inc. | Multivalent antigen-binding proteins |
| EP0305481B1 (de) * | 1987-03-20 | 1993-09-29 | Creative Biomolecules, Inc. | Leader-anteile zur produktion von rekombinanten proteinen |
| EP0335400B1 (de) * | 1988-03-30 | 1994-06-08 | Hitachi, Ltd. | Verfahren zur Herstellung von Epidermal-Wachstumsfaktor durch genetische Transformation |
| US5156968A (en) * | 1988-06-24 | 1992-10-20 | Genentech, Inc. | Purified yeast ubiquitin hydrolase |
| US5262322A (en) * | 1988-06-24 | 1993-11-16 | Genentech, Inc. | Host transformed with yeast gene and ubiquitin/polypeptide fusions |
| US5108919A (en) * | 1988-06-24 | 1992-04-28 | Genentech, Inc. | Dna sequences encoding yeast ubiquitin hydrolase |
| DE69001248T2 (de) * | 1989-05-04 | 1993-10-14 | Genentech Inc | Verfahren und zusammensetzungen zur isolation von menschlichem relaxin. |
| US5595887A (en) * | 1990-07-16 | 1997-01-21 | Bionebraska, Inc. | Purification directed cloning of peptides using carbonic anhydrase as the affinity binding segment |
| DE4105480A1 (de) * | 1991-02-21 | 1992-08-27 | Boehringer Mannheim Gmbh | Verbesserte aktivierung von rekombinanten proteinen |
| US5837491A (en) * | 1991-11-04 | 1998-11-17 | Xoma Corporation | Polynucleotides encoding gelonin sequences |
| US5621083A (en) * | 1991-11-04 | 1997-04-15 | Xoma Corporation | Immunotoxins comprising ribosome-inactivating proteins |
| US6146850A (en) * | 1991-11-04 | 2000-11-14 | Xoma Corporation | Proteins encoding gelonin sequences |
| US6027725A (en) * | 1991-11-25 | 2000-02-22 | Enzon, Inc. | Multivalent antigen-binding proteins |
| US5656456A (en) * | 1992-07-13 | 1997-08-12 | Bionebraska, Inc. | Chemical method for selective modification of the N- and/or C-terminal amino acid α-carbon reactive group of a recombinant polypeptide or a portion thereof |
| DE69332377T2 (de) * | 1992-07-13 | 2003-07-03 | Bionebraska, Inc. | Verfahren zur modifizierung rekombinanter polypeptide |
| AU5670194A (en) * | 1992-11-20 | 1994-06-22 | Enzon, Inc. | Linker for linked fusion polypeptides |
| AU4415796A (en) * | 1994-12-07 | 1996-06-26 | Bionebraska, Inc. | Production of c-terminal amidated peptides from recombinant protein constructs |
| CA2206848A1 (en) * | 1994-12-07 | 1996-06-13 | Bionebraska, Inc. | Production of peptides using recombinant fusion protein constructs |
| GB9716790D0 (en) | 1997-08-07 | 1997-10-15 | Creative Peptides Sweden Ab | Recombinant DNA molecules comprising multimeric copies of a gene sequence and expression thereof |
| KR100620554B1 (ko) | 2004-06-05 | 2006-09-06 | 한국생명공학연구원 | Ｔａｇ-72에 대한 인간화 항체 |
| US7951559B2 (en) | 2007-07-25 | 2011-05-31 | E.I. Du Pont De Nemours And Company | Recombinant peptide production using a cross-linkable solubility tag |
| KR101338517B1 (ko) | 2011-04-18 | 2013-12-10 | 연세대학교 산학협력단 | 인간 간-카르복실에스터라제 １을 특이적으로 인식하는 단일클론 항체, 상기 항체를 생산하는 하이브리도마 세포주 및 이의 용도 |
| US9758571B2 (en) | 2012-07-20 | 2017-09-12 | University—Industry Cooperation Group Of Kyung Hee University | Antibody for epitope tagging, hybridoma cell line and uses thereof |
| CN105636976A (zh) | 2012-07-20 | 2016-06-01 | 庆熙大学校产学协力团 | 新型肽标签以及其用途 |
| KR101453462B1 (ko) | 2013-05-16 | 2014-10-23 | 앱클론(주) | Her2에 특이적으로 결합하는 항체 |
| KR101605421B1 (ko) | 2014-03-05 | 2016-03-23 | 국립암센터 | B 세포 림프종 세포를 특이적으로 인지하는 단일클론항체 및 이의 용도 |
| CN107108743B (zh) | 2014-12-03 | 2021-05-04 | (株)爱恩德生物 | 结合神经毡蛋白1的抗体及其用途 |
| KR102462811B1 (ko) | 2015-04-27 | 2022-11-04 | 에이비온 주식회사 | 항체에 덴드론이 접합된 면역접합체 및 이의 용도 |
| KR101896882B1 (ko) | 2015-11-30 | 2018-09-11 | 앱클론(주) | Vegfr2에 특이적으로 결합하는 항체 |
| JP6795603B2 (ja) | 2016-02-15 | 2020-12-02 | サムソン ライフ パブリック ウェルフェア ファウンデーション | ＥＧＦＲｖＩＩＩ（Ｅｐｉｄｅｒｍａｌ Ｇｒｏｗｔｈ Ｆａｃｔｏｒ Ｒｅｃｅｐｔｏｒ Ｖａｒｉａｎｔ ＩＩＩ）に対する抗体およびその用途 |
| WO2017204606A1 (ko) | 2016-05-27 | 2017-11-30 | 오름테라퓨틱 주식회사 | 세포질 침투 항체 및 이의 용도 |
| EP3467508A4 (de) | 2016-06-03 | 2020-04-29 | Samsung Life Public Welfare Foundation | Anti-nrp1-antikörper-screening-verfahren |
| AU2017273169B2 (en) | 2016-06-03 | 2020-07-30 | Aimed Bio Inc. | Method for screening antibody using patient-derived tumor spheroids |
| WO2017209554A2 (ko) | 2016-06-03 | 2017-12-07 | 사회복지법인 삼성생명공익재단 | 항-nrp1 항체 스크리닝 방법 |
| WO2017209553A2 (ko) | 2016-06-03 | 2017-12-07 | 사회복지법인 삼성생명공익재단 | 환자 유래 세포를 이용한 항체 스크리닝 방법 |
| AU2017306506B2 (en) | 2016-08-05 | 2020-05-21 | Y-Biologics Inc. | Antibody to programmed cell death 1 (PD-1) and use thereof |
| WO2018026249A1 (ko) | 2016-08-05 | 2018-02-08 | 주식회사 와이바이오로직스 | 프로그램화된 세포 사멸 단백질 리간드-1 (pd-l1)에 대한 항체 및 이의 용도 |
| RU2721582C1 (ru) | 2016-08-05 | 2020-05-20 | И-Байолоджикс Инк. | Антитела против лиганда-1 запрограммированной смерти (PD-L1) и их применение |
| KR101757346B1 (ko) | 2017-03-27 | 2017-07-26 | 아주대학교산학협력단 | 항-emap ii 항체 및 이의 용도 |
| KR101970327B1 (ko) | 2017-04-13 | 2019-04-18 | 고려대학교 산학협력단 | 검출 신호의 자가 증폭 원리를 이용한 정확, 신속, 편리한 단일 단계 질병 진단 방법 |
| AU2018278730B2 (en) | 2017-05-30 | 2021-02-04 | Chong Kun Dang Pharmaceutical Corp. | A novel anti-c-Met antibody and use thereof |
| US11306141B2 (en) | 2017-09-08 | 2022-04-19 | Y-Biologics Inc. | Antibody against human DLK1 and use thereof |
| MX2020005495A (es) | 2017-10-20 | 2021-01-08 | Pharmabcine Inc | Anticuerpo anti-vista y uso del mismo. |
| KR102311838B1 (ko) | 2017-12-27 | 2021-10-14 | 주식회사 파멥신 | 항-pd-l1 항체 및 이의 용도 |
| KR102275930B1 (ko) | 2018-03-14 | 2021-07-12 | (주)알테오젠 | Folr1에 특이적으로 결합하는 항체 및 그의 용도 |
| JP7527641B2 (ja) | 2018-04-18 | 2024-08-05 | アブクロン・インコーポレイテッド | スイッチ分子及びスイッチャブルキメラ抗原受容体 |
| CN112469826B (zh) | 2018-04-27 | 2024-02-09 | (株)爱恩德生物 | 通过磁珠附着至细胞进行的基于磁性的生物淘选方法 |
| KR102115300B1 (ko) | 2018-06-01 | 2020-05-26 | 재단법인 목암생명과학연구소 | 항체 라이브러리 및 이를 이용한 항체 스크리닝 방법 |
| KR102842215B1 (ko) | 2018-08-10 | 2025-08-05 | 주식회사 유틸렉스 | Hla-dr에 결합하는 키메라 항원 수용체 및 car-t 세포 |
| KR102353568B1 (ko) | 2018-11-14 | 2022-01-20 | 주식회사 헬릭스미스 | 안정성이 향상된 항 c-Met 항체 또는 그의 항원 결합 단편 |
| MX2021010453A (es) | 2019-03-06 | 2021-09-21 | Legochem Biosciences Inc | Conjugados anticuerpo-farmaco que comprenden anticuerpos contra dlk1 humana y uso de estos. |
| KR102502287B1 (ko) | 2020-04-17 | 2023-02-22 | 앱클론(주) | 항-her2 어피바디 및 이를 스위치 분자로 이용하는 스위처블 키메라 항원 수용체 |
| JP2023527462A (ja) | 2020-06-01 | 2023-06-28 | イノベイション バイオ カンパニー リミテッド | Ｃｄ２２に特異的な抗体およびその使用 |
| EP4163298A4 (de) | 2020-06-09 | 2024-06-12 | Kookmin University Industry Academy Cooperation Foundation | Antikörper mit spezifischer bindung an grp94 oder antigenbindendes fragment davon und verwendungen davon |
| KR102757518B1 (ko) | 2020-12-07 | 2025-01-21 | (주)이노베이션바이오 | Cd47에 특이적인 항체 및 이의 용도 |
| EP4279510A4 (de) | 2021-01-12 | 2025-12-03 | Sg Medical Inc | Neuartiger antikörper gegen cd55 und verwendungen davon |
| JP7651145B2 (ja) | 2021-04-20 | 2025-03-26 | コリア ユニバーシティ リサーチ アンド ビジネス ファウンデーション | 向上した癌細胞死滅効能を有する非対称抗体 |
| US20250129155A1 (en) | 2021-06-30 | 2025-04-24 | Innobation Bio Co., Ltd. | Humanized antibody specific for cd47 and pharmaceutical composition for preventing or treating cd47-related diseases comprising the same |
| WO2023080695A1 (ko) | 2021-11-05 | 2023-05-11 | 서울대학교병원 | 리지스틴 특이적 항체 및 이의 용도 |
| KR102530111B1 (ko) | 2022-03-11 | 2023-05-10 | 앱클론(주) | 코티닌을 표적하는 항체 또는 그의 항원 결합 단편, 이를 포함하는 키메라 항원 수용체 및 이들의 용도 |
| WO2023182866A1 (ko) | 2022-03-25 | 2023-09-28 | 에이치케이이노엔 주식회사 | Hla-g 특이적 항체 및 이의 용도 |
| KR20240008997A (ko) | 2022-07-12 | 2024-01-22 | (재) 스크립스코리아항체연구원 | 사스-코로나 바이러스 2 중화 항체 |
| WO2024063526A1 (ko) | 2022-09-21 | 2024-03-28 | 연세대학교 산학협력단 | 섬유화 질환의 예방 또는 치료용 신규 항체 |
| KR20240114678A (ko) | 2023-01-16 | 2024-07-24 | 주식회사 드노보 바이오테라퓨틱스 | 신규한 항-NKp46 항체 및 이의 용도 |
| KR20240164748A (ko) | 2023-05-12 | 2024-11-20 | 앱클론(주) | 항체 또는 그의 항원 결합 단편, 및 어피바디를 포함하는 이중 항원 표적 단백질 복합체 |
| EP4714977A1 (de) | 2023-05-19 | 2026-03-25 | Seoul National University R&DB Foundation | Verwendung von anti-cd3-antikörpern zur selektiven abreicherung aktivierter t-zellen |
| KR20240170605A (ko) | 2023-05-23 | 2024-12-04 | 연세대학교 산학협력단 | 신규한 인간 인터류킨-4 수용체 결합 나노바디 및 이를 포함하는 비강 분무 제형 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1200773A (en) * | 1980-02-29 | 1986-02-18 | William J. Rutter | Expression linkers |
| EP0070675A1 (de) * | 1981-07-15 | 1983-01-26 | Celltech Therapeutics Limited | Strukturiertes Gen für die Polyprotein-vorstufe des menschlichen Calcitonins |
| EP0108132A1 (de) * | 1982-05-06 | 1984-05-16 | Applied Molecular Genetics Inc. | Herstellung und expression von genen für urogastron und dessen polypeptidanalogen |
| EP0207044A1 (de) * | 1985-06-20 | 1986-12-30 | Monsanto Company | Freisetzung von Peptiden aus Polypeptiden |
-
1988
- 1988-03-04 AU AU15750/88A patent/AU605291B2/en not_active Expired
- 1988-03-04 EP EP94200625A patent/EP0621340A1/de not_active Withdrawn
- 1988-03-04 DE DE3852074T patent/DE3852074D1/de not_active Expired - Lifetime
- 1988-03-04 JP JP63503161A patent/JPH02500876A/ja active Pending
- 1988-03-04 EP EP88903520A patent/EP0305500B1/de not_active Expired - Lifetime
- 1988-03-04 WO PCT/US1988/000717 patent/WO1988007085A1/en not_active Ceased
- 1988-03-04 AT AT88903520T patent/ATE113985T1/de active
- 1988-03-18 CA CA000561913A patent/CA1339208C/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| EP0305500A1 (de) | 1989-03-08 |
| CA1339208C (en) | 1997-08-05 |
| AU605291B2 (en) | 1991-01-10 |
| EP0305500B1 (de) | 1994-11-09 |
| AU1575088A (en) | 1988-10-10 |
| EP0621340A1 (de) | 1994-10-26 |
| DE3852074D1 (de) | 1994-12-15 |
| WO1988007085A1 (en) | 1988-09-22 |
| JPH02500876A (ja) | 1990-03-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE3852074D1 (de) | Verfahren zur reinigung von rekombinanten polypeptiden. | |
| DE3477810D1 (en) | Polypeptide and protein products, and processes for their production and use | |
| KR920701452A (ko) | 프라세싱 부위에 인접한 음전하를 띤 아미노산을 함유하는 이스트 프라세싱 시스템 | |
| DK161681A (da) | Fremgangsmaade til syntese i en bakteriel vaert af et udvalgt modent protein eller polypeptid | |
| ATE124456T1 (de) | Verfahren zur enzymatischen spaltung rekombinanter proteine unter verwendung von iga- proteasen. | |
| EP0722461A4 (de) | Expression eines fusionspolypeptides, das ohne leadersequenz aus dem cytoplasma transportiert wird | |
| DK38189A (da) | Hidtil ukendte fusionsproteiner indeholdende glutation-s-transferase | |
| DE3586333D1 (de) | Expressionsvektoren fuer hirudin, transformierte zellen und verfahren zur herstellung von hirudin. | |
| ATE123520T1 (de) | Kex2-endoprotease und verfahren zu ihrer herstellung. | |
| ES2000278A6 (es) | Procedimiento para preparar una proteina de fusion y para preparar una proteina eucariotica. | |
| FI880017A0 (fi) | Menetelmä haiman erittämän trypsiini-inhibiittorivarianttien valmistamiseksi ja menetelmässä käytettävä DNA, vektori ja isäntäsolu | |
| ATE108205T1 (de) | Rekombinantverfahren zur herstellung von serinproteaseinhibitoren, sowie dns-sequenzen dazu. | |
| SE8204811L (sv) | Dna-fragment innefattande en signalsekvens | |
| EA200000202A1 (ru) | Рекомбинантная экспрессия c-пептида инсулина | |
| DE3877035D1 (de) | Gentechnologisches verfahren zur herstellung von polypeptiden. | |
| FI830813L (fi) | Foerfarande foer omvandling av pre-proinsulinanaloger till insulin | |
| ES2078229T3 (es) | Procedimiento para la preparacion de un polipeptido similar a la motilina y su expresion. | |
| ATE78873T1 (de) | Verfahren zur selektiven spaltung von fusionsproteinen. | |
| ATE103983T1 (de) | Licht-induzierbarer promotor. | |
| NO900410L (no) | Fremgangsmaate til produksjon av glukagon. | |
| ATE105584T1 (de) | Biosynthetisches verfahren zur herstellung von chemischen verbindungen. | |
| DK0716705T3 (da) | Fremgangsmåde til rekombinant fremstilling af proteiner i gæren Hansenula | |
| FI870501A0 (fi) | Menetelmä melanoomaan liittyvän p97-antigeenin antigeenisen sukulaispeptidin tai -proteiinin valmistamiseksi, sekä siinä käyttökelpoiset yhdistelmävirukset, yhdistelmävektorit ja isäntäsolut | |
| DK0725822T3 (da) | Fremgangsmåde til rekombinant fremstilling af proteiner i gær | |
| ATE139538T1 (de) | Verfahren zur selektiven spaltung von fusionsproteinen |