ATE113985T1 - Verfahren zur reinigung von rekombinanten polypeptiden. - Google Patents
Verfahren zur reinigung von rekombinanten polypeptiden.Info
- Publication number
- ATE113985T1 ATE113985T1 AT88903520T AT88903520T ATE113985T1 AT E113985 T1 ATE113985 T1 AT E113985T1 AT 88903520 T AT88903520 T AT 88903520T AT 88903520 T AT88903520 T AT 88903520T AT E113985 T1 ATE113985 T1 AT E113985T1
- Authority
- AT
- Austria
- Prior art keywords
- cleavage site
- purification
- fused
- amino acids
- leader sequence
- Prior art date
Links
- 229920001184 polypeptide Polymers 0.000 title abstract 5
- 102000004196 processed proteins & peptides Human genes 0.000 title abstract 5
- 108090000765 processed proteins & peptides Proteins 0.000 title abstract 5
- 238000000034 method Methods 0.000 title abstract 4
- 238000000746 purification Methods 0.000 title abstract 3
- 238000003776 cleavage reaction Methods 0.000 abstract 5
- 230000007017 scission Effects 0.000 abstract 5
- 150000001413 amino acids Chemical class 0.000 abstract 2
- 102000005593 Endopeptidases Human genes 0.000 abstract 1
- 108010059378 Endopeptidases Proteins 0.000 abstract 1
- 108020004511 Recombinant DNA Proteins 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 238000002955 isolation Methods 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/485—Epidermal growth factor [EGF], i.e. urogastrone
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/585—Calcitonins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Medicinal Chemistry (AREA)
- Toxicology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Endocrinology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US2848487A | 1987-03-20 | 1987-03-20 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE113985T1 true ATE113985T1 (de) | 1994-11-15 |
Family
ID=21843701
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT88903520T ATE113985T1 (de) | 1987-03-20 | 1988-03-04 | Verfahren zur reinigung von rekombinanten polypeptiden. |
Country Status (7)
| Country | Link |
|---|---|
| EP (2) | EP0621340A1 (de) |
| JP (1) | JPH02500876A (de) |
| AT (1) | ATE113985T1 (de) |
| AU (1) | AU605291B2 (de) |
| CA (1) | CA1339208C (de) |
| DE (1) | DE3852074D1 (de) |
| WO (1) | WO1988007085A1 (de) |
Families Citing this family (69)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4946778A (en) * | 1987-09-21 | 1990-08-07 | Genex Corporation | Single polypeptide chain binding molecules |
| US6121424A (en) * | 1991-11-25 | 2000-09-19 | Enzon, Inc. | Multivalent antigen-binding proteins |
| US5260203A (en) * | 1986-09-02 | 1993-11-09 | Enzon, Inc. | Single polypeptide chain binding molecules |
| US5869620A (en) * | 1986-09-02 | 1999-02-09 | Enzon, Inc. | Multivalent antigen-binding proteins |
| WO1988007086A1 (en) * | 1987-03-20 | 1988-09-22 | Creative Biomolecules, Inc. | Leader sequences for the production of recombinant proteins |
| DE68915841T2 (de) * | 1988-03-30 | 1995-02-02 | Hitachi Chemical Co Ltd | Verfahren zur Herstellung von Epidermal-Wachstumsfaktor durch genetische Transformation. |
| US5108919A (en) * | 1988-06-24 | 1992-04-28 | Genentech, Inc. | Dna sequences encoding yeast ubiquitin hydrolase |
| US5262322A (en) * | 1988-06-24 | 1993-11-16 | Genentech, Inc. | Host transformed with yeast gene and ubiquitin/polypeptide fusions |
| US5156968A (en) * | 1988-06-24 | 1992-10-20 | Genentech, Inc. | Purified yeast ubiquitin hydrolase |
| CA2051375C (en) * | 1989-05-04 | 2000-09-12 | Dennis J. Henner | Process and compositions for the isolation of human relaxin |
| US5595887A (en) * | 1990-07-16 | 1997-01-21 | Bionebraska, Inc. | Purification directed cloning of peptides using carbonic anhydrase as the affinity binding segment |
| DE4105480A1 (de) * | 1991-02-21 | 1992-08-27 | Boehringer Mannheim Gmbh | Verbesserte aktivierung von rekombinanten proteinen |
| US5621083A (en) | 1991-11-04 | 1997-04-15 | Xoma Corporation | Immunotoxins comprising ribosome-inactivating proteins |
| US6146850A (en) * | 1991-11-04 | 2000-11-14 | Xoma Corporation | Proteins encoding gelonin sequences |
| US5837491A (en) * | 1991-11-04 | 1998-11-17 | Xoma Corporation | Polynucleotides encoding gelonin sequences |
| ATE225801T1 (de) * | 1992-07-13 | 2002-10-15 | Bionebraska Inc | Verfahren zur modifizierung rekombinanter polypeptide |
| US5656456A (en) * | 1992-07-13 | 1997-08-12 | Bionebraska, Inc. | Chemical method for selective modification of the N- and/or C-terminal amino acid α-carbon reactive group of a recombinant polypeptide or a portion thereof |
| AU5670194A (en) * | 1992-11-20 | 1994-06-22 | Enzon, Inc. | Linker for linked fusion polypeptides |
| WO1996017941A2 (en) * | 1994-12-07 | 1996-06-13 | Bionebraska, Inc. | Production of c-terminal amidated peptides from recombinant protein constructs |
| AU700605B2 (en) * | 1994-12-07 | 1999-01-07 | Bionebraska, Inc. | Production of peptides using recombinant fusion protein constructs |
| GB9716790D0 (en) | 1997-08-07 | 1997-10-15 | Creative Peptides Sweden Ab | Recombinant DNA molecules comprising multimeric copies of a gene sequence and expression thereof |
| KR100620554B1 (ko) | 2004-06-05 | 2006-09-06 | 한국생명공학연구원 | Ｔａｇ-72에 대한 인간화 항체 |
| US7951559B2 (en) | 2007-07-25 | 2011-05-31 | E.I. Du Pont De Nemours And Company | Recombinant peptide production using a cross-linkable solubility tag |
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| CN105636976A (zh) | 2012-07-20 | 2016-06-01 | 庆熙大学校产学协力团 | 新型肽标签以及其用途 |
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| MY192630A (en) | 2017-05-30 | 2022-08-29 | Chong Kun Dang Pharmaceutical Corp | A novel anti-c-met antibody and use thereof |
| US11306141B2 (en) | 2017-09-08 | 2022-04-19 | Y-Biologics Inc. | Antibody against human DLK1 and use thereof |
| WO2019078699A2 (ko) | 2017-10-20 | 2019-04-25 | 주식회사 파멥신 | 항-vista 항체 및 이의 용도 |
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| CN111989342B (zh) | 2018-04-18 | 2024-08-23 | 艾克隆株式会社 | 开关分子以及可转换嵌合抗原受体 |
| CA3096533C (en) | 2018-04-27 | 2023-08-29 | Aimed Bio Inc. | Magnetic-based biopanning method through attachment of magnetic bead to cell |
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| BR112021002487A2 (pt) | 2018-08-10 | 2021-07-27 | Eutilex Co., Ltd. | receptor de antígeno quimérico que se liga a hla-dr e célula car-t |
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| US20220339291A1 (en) | 2019-03-06 | 2022-10-27 | Legochem Biosciences, Inc. | Antibody-drug conjugates that target dlk1 and uses thereof |
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Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1200773A (en) * | 1980-02-29 | 1986-02-18 | William J. Rutter | Expression linkers |
| WO1983000346A1 (en) * | 1981-07-15 | 1983-02-03 | Roger Kingdon Craig | Human calcitonin precursor polyprotein structural gene |
| EP0108132A1 (de) * | 1982-05-06 | 1984-05-16 | Applied Molecular Genetics Inc. | Herstellung und expression von genen für urogastron und dessen polypeptidanalogen |
| EP0207044A1 (de) * | 1985-06-20 | 1986-12-30 | Monsanto Company | Freisetzung von Peptiden aus Polypeptiden |
-
1988
- 1988-03-04 EP EP94200625A patent/EP0621340A1/de not_active Withdrawn
- 1988-03-04 AU AU15750/88A patent/AU605291B2/en not_active Expired
- 1988-03-04 EP EP88903520A patent/EP0305500B1/de not_active Expired - Lifetime
- 1988-03-04 AT AT88903520T patent/ATE113985T1/de active
- 1988-03-04 WO PCT/US1988/000717 patent/WO1988007085A1/en not_active Ceased
- 1988-03-04 DE DE3852074T patent/DE3852074D1/de not_active Expired - Lifetime
- 1988-03-04 JP JP63503161A patent/JPH02500876A/ja active Pending
- 1988-03-18 CA CA000561913A patent/CA1339208C/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| CA1339208C (en) | 1997-08-05 |
| EP0305500B1 (de) | 1994-11-09 |
| EP0621340A1 (de) | 1994-10-26 |
| AU605291B2 (en) | 1991-01-10 |
| EP0305500A1 (de) | 1989-03-08 |
| AU1575088A (en) | 1988-10-10 |
| DE3852074D1 (de) | 1994-12-15 |
| JPH02500876A (ja) | 1990-03-29 |
| WO1988007085A1 (en) | 1988-09-22 |
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