ATE117720T1 - Aspartat-proteinase-defiziente filamentöse fungi. - Google Patents
Aspartat-proteinase-defiziente filamentöse fungi.Info
- Publication number
- ATE117720T1 ATE117720T1 AT89908939T AT89908939T ATE117720T1 AT E117720 T1 ATE117720 T1 AT E117720T1 AT 89908939 T AT89908939 T AT 89908939T AT 89908939 T AT89908939 T AT 89908939T AT E117720 T1 ATE117720 T1 AT E117720T1
- Authority
- AT
- Austria
- Prior art keywords
- filamentous fungi
- deficiency
- aspartate proteinase
- proteinase
- aspartate
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6478—Aspartic endopeptidases (3.4.23)
- C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF
- A23C19/00—Cheese; Cheese preparations; Making thereof
- A23C19/02—Making cheese curd
- A23C19/04—Making cheese curd characterised by the use of specific enzymes of vegetable or animal origin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungi isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
- C12N9/62—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from Aspergillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/66—Aspergillus
- C12R2001/665—Aspergillus awamori
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Molecular Biology (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Biophysics (AREA)
- Botany (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Chemical Or Physical Treatment Of Fibers (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Materials For Medical Uses (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US21423788A | 1988-07-01 | 1988-07-01 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE117720T1 true ATE117720T1 (de) | 1995-02-15 |
Family
ID=22798311
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT89908939T ATE117720T1 (de) | 1988-07-01 | 1989-07-01 | Aspartat-proteinase-defiziente filamentöse fungi. |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US5840570A (de) |
| EP (1) | EP0429490B2 (de) |
| AT (1) | ATE117720T1 (de) |
| CA (1) | CA1333777C (de) |
| DE (1) | DE68920882T3 (de) |
| FI (1) | FI906442A7 (de) |
| HK (1) | HK110395A (de) |
| WO (1) | WO1990000192A1 (de) |
Families Citing this family (68)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3133774B2 (ja) * | 1990-10-04 | 2001-02-13 | 日本食品化工株式会社 | 新規な宿主−ベクター系 |
| DE69121159T2 (de) * | 1990-10-04 | 1997-03-13 | Japan Maize Prod | Wirt-Vektor-System |
| US5997913A (en) * | 1990-12-10 | 1999-12-07 | Genencor International, Inc. | Method enhancing flavor and aroma in foods by overexpression of β-glucosidase |
| US5846802A (en) * | 1992-04-15 | 1998-12-08 | Buxton; Frank | Fungal Protease |
| DE69333249T2 (de) * | 1992-04-15 | 2004-07-29 | Novartis Ag | Neue Schimmel-Protease |
| US5674728A (en) * | 1993-11-03 | 1997-10-07 | Novartis Corporation | Aspergillus niger vacuolar aspartyl protease |
| JPH10501414A (ja) * | 1994-06-17 | 1998-02-10 | ノボ ノルディスク アクティーゼルスカブ | areA遺伝子が修飾された真菌及びアスペルギルス オリザエからのareA遺伝子 |
| NL9401678A (nl) * | 1994-10-12 | 1996-05-01 | Evert Hovius | Methode van de zichzelf stabiliserende lus (Z.Z.S.L.). |
| ES2281079T3 (es) * | 1995-03-20 | 2007-09-16 | Novozymes A/S | Celula huesped que expresa niveles reducidos de metaloproteasa y metodos de utilizacion de la celula huesped para la produccion de proteinas. |
| WO1997022705A1 (en) * | 1995-12-15 | 1997-06-26 | Novo Nordisk A/S | A FUNGUNS WHEREIN THE areA, pepC AND/OR pepE GENES HAVE BEEN INACTIVATED |
| WO1997035956A1 (en) * | 1996-03-27 | 1997-10-02 | Novo Nordisk A/S | Alkaline protease deficient filamentous fungi |
| JP2000511429A (ja) * | 1996-06-05 | 2000-09-05 | ギスト ブロカデス ベスローテン フェンノートシャップ | 真菌メタロプロテアーゼ遺伝子 |
| CN1262639C (zh) | 1996-09-19 | 2006-07-05 | 诺沃奇梅兹有限公司 | 新的宿主细胞和生产蛋白质的方法 |
| WO1999060136A1 (en) * | 1998-05-15 | 1999-11-25 | Novo Nordisk Biotech, Inc. | Methods for producing polypeptides in filamentous fungal mutant cells |
| US6806062B1 (en) | 1998-10-05 | 2004-10-19 | Novozymes A/S | Fungal transcriptional activator useful in methods for producing polypeptides |
| EP2009098A1 (de) | 1999-07-09 | 2008-12-31 | Novozymes A/S | Variante der Glucoamylase |
| AU2001242299A1 (en) | 2000-03-14 | 2001-09-24 | Novozymes A/S | Fungal transcriptional activator useful in methods for producing polypeptides |
| AU2000260261A1 (en) * | 2000-07-03 | 2002-01-14 | Wageningen University | Method for the production of proteins and polypeptides using fungal cells, in particular aspergillus vadensis |
| US20040219609A1 (en) * | 2002-07-12 | 2004-11-04 | Day Anthony G. | Methods for modulating proteins not previously known as proteases |
| CA2498319A1 (en) * | 2002-09-09 | 2004-03-18 | Nautilus Biotech | Rational evolution of cytokines for higher stability, the cytokines and encoding nucleic acid molecules |
| EP1852441B1 (de) * | 2002-09-24 | 2012-03-28 | The Burnham Institute | Mittel zur Modulierung der EPH-Rezeptor-Aktivität |
| EP1553963A4 (de) * | 2002-09-24 | 2006-05-03 | Burnham Inst | NEUE MITTEL ZUR MODULIERUNG DER EPH-REZEPTOR-AKTIVITûT |
| KR20120099303A (ko) * | 2003-11-06 | 2012-09-07 | 다니스코 유에스 인크. | 프로테아제 저해자 및 그 변이체의 사상균 내에서의 발현 |
| CA2546451A1 (en) | 2003-11-19 | 2005-06-09 | Genencor International, Inc. | Serine proteases, nucleic acids encoding serine enzymes and vectors and host cells incorporating same |
| US7985569B2 (en) | 2003-11-19 | 2011-07-26 | Danisco Us Inc. | Cellulomonas 69B4 serine protease variants |
| US7998930B2 (en) | 2004-11-04 | 2011-08-16 | Hanall Biopharma Co., Ltd. | Modified growth hormones |
| WO2006066595A2 (en) * | 2004-12-22 | 2006-06-29 | Novozymes A/S | Recombinant production of serum albumin |
| EP2363460A3 (de) * | 2004-12-30 | 2011-12-28 | Genencor International, Inc. | Säureaktive fungale Protease |
| PL2410048T3 (pl) | 2005-01-24 | 2017-02-28 | Dsm Ip Assets B.V. | Sposób otrzymywania związku będącego przedmiotem zainteresowania w komórce grzyba nitkowatego |
| AU2006208068B2 (en) | 2005-01-27 | 2011-08-04 | The Burnham Institute | EphB receptor-binding peptides |
| ES2375150T3 (es) | 2005-03-16 | 2012-02-27 | Novozymes Adenium Biotech A/S | Expresión recombinante de defensinas de insecto en aspergillus. |
| CN101146907B (zh) | 2005-04-12 | 2015-04-08 | 金克克国际有限公司 | 蛋白质生产被改变的基因失活突变体 |
| US7858360B2 (en) | 2005-10-17 | 2010-12-28 | Novozymes A/S | Use of fungal mutants for expression of antibodies |
| US7688029B2 (en) * | 2005-11-08 | 2010-03-30 | Eveready Battery Company, Inc. | Portable battery powered appliance and method of operation |
| US20080280328A1 (en) | 2005-11-18 | 2008-11-13 | Novozymes A/S | Glucoamylase Variants |
| US7776339B2 (en) | 2006-01-11 | 2010-08-17 | Active Organics, Lp | Extract of R. miehei |
| EP2035561A1 (de) | 2006-06-29 | 2009-03-18 | DSMIP Assets B.V. | Verfahren für verbesserte polypeptidexpression |
| US20080026376A1 (en) | 2006-07-11 | 2008-01-31 | Huaming Wang | KEX2 cleavage regions of recombinant fusion proteins |
| US8198046B2 (en) * | 2006-07-11 | 2012-06-12 | Danisco Us Inc. | KEX2 cleavage regions of recombinant fusion proteins |
| CN101517084B (zh) * | 2006-09-22 | 2014-12-10 | 丹尼斯科美国公司 | 来自里氏木霉的乙酰乳酸合酶(als)选择性标记 |
| KR20090115743A (ko) * | 2007-02-28 | 2009-11-05 | 다니스코 유에스 인크. | 알파-갈락토시다아제를 포함하는 세정 조성물 |
| CA2698765A1 (en) * | 2007-09-07 | 2009-03-19 | Danisco Us Inc. | Beta-glucosidase enhanced filamentous fungal whole cellulase compositions and methods of use |
| DK2195435T3 (da) * | 2007-09-12 | 2012-12-10 | Danisco Us Inc | Trichoderma-promoter |
| US20110039320A1 (en) * | 2007-10-12 | 2011-02-17 | Mian Li | Methods and Compositions for Enhanced Production of Organic Substances From Fermenting Microorganisms |
| US8633010B2 (en) | 2007-12-06 | 2014-01-21 | Novozymes A/S | Fungal PepC inhibitor |
| MX2010010144A (es) * | 2008-03-21 | 2010-10-20 | Danisco Us Inc | Composiciones enriquecidas con hemicelulasa para incrementar la hidrolisis de biomasa. |
| US20090253173A1 (en) * | 2008-04-08 | 2009-10-08 | Danisco Us Inc., Genencor Division | Filamentous fungi with inactivated protease genes for altered protein production |
| EP2398889B1 (de) | 2009-02-20 | 2018-04-25 | Danisco US Inc. | Fermentationsbrühenformulierungen |
| KR20120115206A (ko) | 2009-09-23 | 2012-10-17 | 다니스코 유에스 인크. | 신규 글리코실 히드롤라제 효소 및 이의 용도 |
| WO2011073883A1 (en) | 2009-12-14 | 2011-06-23 | Centre Hospitalier Universitaire Vaudois (Chuv) | Pichia pastoris deficient in endogenous secreted protease |
| MX350399B (es) * | 2009-12-18 | 2017-09-06 | Novozymes Inc | Metodos para producir polipeptidos en mutantes de trichoderma deficientes en proteasa. |
| KR20120106774A (ko) | 2009-12-23 | 2012-09-26 | 다니스코 유에스 인크. | 동시 당화 발효 반응의 효율을 향상시키는 방법 |
| CN103443268A (zh) | 2011-01-20 | 2013-12-11 | 诺维信公司 | 植物过氧化物酶在丝状真菌中的表达 |
| WO2012125925A2 (en) | 2011-03-17 | 2012-09-20 | Danisco Us Inc. | Method for reducing viscosity in saccharification process |
| WO2013048661A1 (en) | 2011-09-30 | 2013-04-04 | Codexis, Inc. | Fungal proteases |
| WO2013102674A2 (en) | 2012-01-05 | 2013-07-11 | Novartis International Pharmaceutical Ltd. | Protease deficient filamentous fungal cells and methods of use thereof |
| DK2852610T3 (en) | 2012-05-23 | 2018-09-03 | Glykos Finland Oy | PRODUCTION OF FUCOSYLED GLYCOPROTEIN |
| DK3019602T3 (en) | 2013-07-10 | 2018-11-12 | Glykos Finland Oy | MULTIPLE PROTEASE-DEFECTED FILAMENTARY FUNGAL CELLS AND PROCEDURES FOR USE THEREOF |
| AU2015293949B2 (en) | 2014-07-21 | 2019-07-25 | Teknologian Tutkimuskeskus Vtt Oy | Production of glycoproteins with mammalian-like N-glycans in filamentous fungi |
| US10883131B2 (en) | 2015-02-20 | 2021-01-05 | Roal Oy | Methods for controlling protease production |
| CN108779155A (zh) | 2016-04-19 | 2018-11-09 | 诺维信公司 | RlmA灭活的丝状真菌宿主细胞 |
| WO2018093752A1 (en) | 2016-11-15 | 2018-05-24 | Danisco Us Inc. | Filamentous fungi with improved protein production |
| WO2018167153A1 (en) | 2017-03-17 | 2018-09-20 | Novozymes A/S | Improved filamentous fungal host cell |
| WO2018172155A1 (en) | 2017-03-23 | 2018-09-27 | Novozymes A/S | Improved filamentous fungal host cells |
| EP3864136A1 (de) | 2018-10-09 | 2021-08-18 | Novozymes A/S | Modifizierte filamentöse pilzwirtszelle |
| JP7609774B2 (ja) | 2018-11-28 | 2025-01-07 | ノボザイムス アクティーゼルスカブ | 修飾された糸状菌宿主細胞 |
| CN114555777A (zh) | 2019-07-26 | 2022-05-27 | 诺维信公司 | 经修饰的丝状真菌宿主细胞 |
| WO2025230415A1 (en) | 2024-04-30 | 2025-11-06 | Ginkgo Bioworks Netherlands B.V. | Host cells expressing optb protein variants and uses thereof |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NZ208612A (en) * | 1983-06-24 | 1991-09-25 | Genentech Inc | Method of producing "procaryotic carbonyl hydrolases" containing predetermined, site specific mutations |
| US4758512A (en) * | 1984-03-06 | 1988-07-19 | President And Fellows Of Harvard College | Hosts and methods for producing recombinant products in high yields |
| US4828994A (en) * | 1984-09-21 | 1989-05-09 | Genex Corporation | Bacillus strains with reduced extracellular protease levels |
| US4963487A (en) * | 1985-01-03 | 1990-10-16 | Massachusetts Institute Of Technology | Method for deletion of a gene from a bacteria |
| WO1986005812A1 (en) * | 1985-03-29 | 1986-10-09 | Biotechnica International, Inc. | Secretion vector |
| JPS6236183A (ja) * | 1985-06-20 | 1987-02-17 | ザ・サルク・インステイチユ−ト・バイオテクノロジ−/インダストリアル・アソシエイツ・インコ−ポレ−テツド | サツカロミセス・セレビシエからのポリペプチドの発現および分泌 |
| NZ217373A (en) * | 1985-08-29 | 1990-10-26 | Genencor Inc | Production of heterologous peptide in filamentous fungi, vectors and dna |
| JPH0650985B2 (ja) * | 1985-12-27 | 1994-07-06 | サントリー株式会社 | 新規な大腸菌宿主 |
| WO1987004461A1 (en) * | 1986-01-15 | 1987-07-30 | Amgen | THERMALLY STABLE AND pH STABLE SUBTILISIN ANALOGS AND METHOD FOR PRODUCTION THEREOF |
| GB8610600D0 (en) * | 1986-04-30 | 1986-06-04 | Novo Industri As | Transformation of trichoderma |
| FI872102A7 (fi) * | 1986-06-06 | 1987-12-07 | Panlabs Inc | Rihmasienten ilmentymisjärjestelmät. |
| US4709012A (en) | 1986-08-07 | 1987-11-24 | Washington University | Novel inhibitor peptides |
| JPS6387975A (ja) | 1986-10-02 | 1988-04-19 | Agency Of Ind Science & Technol | 枯草菌菌株 |
-
1989
- 1989-06-30 CA CA000604556A patent/CA1333777C/en not_active Expired - Lifetime
- 1989-07-01 WO PCT/US1989/002891 patent/WO1990000192A1/en not_active Ceased
- 1989-07-01 EP EP89908939A patent/EP0429490B2/de not_active Expired - Lifetime
- 1989-07-01 FI FI906442A patent/FI906442A7/fi not_active Application Discontinuation
- 1989-07-01 DE DE68920882T patent/DE68920882T3/de not_active Expired - Lifetime
- 1989-07-01 AT AT89908939T patent/ATE117720T1/de not_active IP Right Cessation
-
1994
- 1994-11-23 US US08/345,018 patent/US5840570A/en not_active Expired - Lifetime
-
1995
- 1995-07-06 HK HK110395A patent/HK110395A/en not_active IP Right Cessation
-
1998
- 1998-03-26 US US09/048,473 patent/US6509171B1/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| EP0429490A1 (de) | 1991-06-05 |
| WO1990000192A1 (en) | 1990-01-11 |
| DE68920882T2 (de) | 1995-08-17 |
| FI906442A0 (fi) | 1990-12-28 |
| DE68920882D1 (de) | 1995-03-09 |
| DE68920882T3 (de) | 2005-06-09 |
| CA1333777C (en) | 1995-01-03 |
| HK110395A (en) | 1995-07-14 |
| EP0429490B1 (de) | 1995-01-25 |
| US5840570A (en) | 1998-11-24 |
| FI906442A7 (fi) | 1990-12-28 |
| EP0429490B2 (de) | 2004-09-22 |
| US6509171B1 (en) | 2003-01-21 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification | ||
| UEP | Publication of translation of european patent specification |
Ref document number: 0429490 Country of ref document: EP |
|
| EELA | Cancelled due to lapse of time |