ATE123521T1 - Hohe expression von proteinen in hefe. - Google Patents
Hohe expression von proteinen in hefe.Info
- Publication number
- ATE123521T1 ATE123521T1 AT89304297T AT89304297T ATE123521T1 AT E123521 T1 ATE123521 T1 AT E123521T1 AT 89304297 T AT89304297 T AT 89304297T AT 89304297 T AT89304297 T AT 89304297T AT E123521 T1 ATE123521 T1 AT E123521T1
- Authority
- AT
- Austria
- Prior art keywords
- yeast
- proteins
- high expression
- adr
- regulatory sequences
- Prior art date
Links
- 240000004808 Saccharomyces cerevisiae Species 0.000 title abstract 3
- 102000004169 proteins and genes Human genes 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 title 1
- 102100034044 All-trans-retinol dehydrogenase [NAD(+)] ADH1B Human genes 0.000 abstract 1
- 101710193111 All-trans-retinol dehydrogenase [NAD(+)] ADH4 Proteins 0.000 abstract 1
- 108010058643 Fungal Proteins Proteins 0.000 abstract 1
- 230000001105 regulatory effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/50—Fibroblast growth factor [FGF]
- C07K14/503—Fibroblast growth factor [FGF] basic FGF [bFGF]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
- C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Mycology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US19086888A | 1988-05-06 | 1988-05-06 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE123521T1 true ATE123521T1 (de) | 1995-06-15 |
Family
ID=22703135
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT89304297T ATE123521T1 (de) | 1988-05-06 | 1989-04-28 | Hohe expression von proteinen in hefe. |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US6183985B1 (de) |
| EP (1) | EP0340986B1 (de) |
| JP (4) | JP3074174B2 (de) |
| AT (1) | ATE123521T1 (de) |
| CA (1) | CA1324969C (de) |
| DE (1) | DE68922932T2 (de) |
| DK (1) | DK175898B1 (de) |
| ES (1) | ES2072900T3 (de) |
Families Citing this family (40)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| PT801682E (pt) | 1995-02-09 | 2002-03-28 | Behringwerke Ag | Processo para a producao de proteinas |
| DE19544233A1 (de) * | 1995-11-28 | 1997-06-05 | Hoechst Ag | Verfahren zur Nutzung des Hefe-ADH II-Promotorsystems zur biotechnologischen Produktion heterologer Proteine in hohen Ausbeuten |
| CN1571838B (zh) * | 2001-09-20 | 2010-05-26 | 丰田自动车株式会社 | 实现基因高表达的系统 |
| BRPI0507169A (pt) | 2004-02-02 | 2007-06-26 | Ambrx Inc | polipeptìdeos do hormÈnio de crescimento humano modificados e seu usos |
| US7632924B2 (en) * | 2004-06-18 | 2009-12-15 | Ambrx, Inc. | Antigen-binding polypeptides and their uses |
| WO2006091231A2 (en) * | 2004-07-21 | 2006-08-31 | Ambrx, Inc. | Biosynthetic polypeptides utilizing non-naturally encoded amino acids |
| EP2399893B1 (de) | 2004-12-22 | 2018-08-15 | Ambrx, Inc. | Zusammensetzungen und Verfahren mit sowie Verwendungen von nichtnatürlichen Aminosäuren und Polypeptiden |
| BRPI0519430A2 (pt) | 2004-12-22 | 2009-02-10 | Ambrx Inc | hormânio do crescimento humano modificado |
| CN103520735B (zh) | 2004-12-22 | 2015-11-25 | Ambrx公司 | 包含非天然编码的氨基酸的人生长激素配方 |
| EP1836316A4 (de) | 2004-12-22 | 2009-07-22 | Ambrx Inc | Verfahren zur expression und reinigung von rekombinantem menschlichem wachstumshormon |
| EP1836298B1 (de) * | 2004-12-22 | 2012-01-18 | Ambrx, Inc. | Zusammensetzungen von aminoacyl-trna-synthetase und verwendungen davon |
| AU2006255122B2 (en) * | 2005-06-03 | 2010-10-21 | Ambrx, Inc. | Improved human interferon molecules and their uses |
| DE102005029170A1 (de) * | 2005-06-23 | 2006-12-28 | Consortium für elektrochemische Industrie GmbH | System zur Genexpression in Hefen der Gattung Sporidiobolus |
| AU2005335491B2 (en) | 2005-08-18 | 2010-11-25 | Ambrx, Inc. | Compositions of tRNA and uses thereof |
| CN101400646A (zh) * | 2005-11-08 | 2009-04-01 | Ambrx公司 | 用于修饰非天然氨基酸和非天然氨基酸多肽的促进剂 |
| DK2339014T3 (en) | 2005-11-16 | 2015-07-20 | Ambrx Inc | Methods and compositions comprising non-natural amino acids |
| ES2514316T3 (es) | 2005-11-22 | 2014-10-28 | Novartis Vaccines And Diagnostics, Inc. | Partículas similares a virus (VLPs) de Norovirus y Sapovirus |
| EP2064333B1 (de) * | 2006-09-08 | 2014-02-26 | Ambrx, Inc. | Unterdrücker-trna-transkription für wirbeltierzellen |
| CN106008699A (zh) * | 2006-09-08 | 2016-10-12 | Ambrx公司 | 经修饰的人类血浆多肽或Fc骨架和其用途 |
| JP5399906B2 (ja) * | 2006-09-08 | 2014-01-29 | アンブルックス，インコーポレイテッド | 脊椎動物細胞用のハイブリッドサプレッサーｔｒｎａ |
| US8143023B2 (en) | 2007-01-26 | 2012-03-27 | Novozymes A/S | Method for methanol independent induction from methanol inducible promoters in Pichia |
| CN107501407B (zh) | 2007-03-30 | 2022-03-18 | Ambrx公司 | 经修饰fgf-21多肽和其用途 |
| US8114630B2 (en) | 2007-05-02 | 2012-02-14 | Ambrx, Inc. | Modified interferon beta polypeptides and their uses |
| MX2010005317A (es) | 2007-11-20 | 2010-06-02 | Ambrx Inc | Polipeptidos de insulina modificados y sus usos. |
| MX344166B (es) | 2008-02-08 | 2016-12-07 | Ambrx Inc | Leptina-polipeptidos modificados y sus usos. |
| CN102105589A (zh) * | 2008-07-11 | 2011-06-22 | 诺维信公司 | 巴斯德毕赤酵母(Pichia pastoris) DAS启动子变体 |
| CN102159230A (zh) | 2008-07-23 | 2011-08-17 | Ambrx公司 | 经修饰的牛g-csf多肽和其用途 |
| AU2009296267B2 (en) | 2008-09-26 | 2013-10-31 | Ambrx, Inc. | Non-natural amino acid replication-dependent microorganisms and vaccines |
| BR122012024318A2 (pt) | 2008-09-26 | 2019-07-30 | Ambrx, Inc. | Polipeptídeos modificados de eritropoetina animal e seus usos |
| BR112012015597A2 (pt) | 2009-12-21 | 2017-01-31 | Ambrx Inc | peptídeos de somatotropina suínos modificados e seus usos |
| MX349301B (es) | 2009-12-21 | 2017-07-21 | Ambrx Inc | Polipéptidos de somatotropina bovina modificados y sus usos. |
| AU2011276328C1 (en) | 2010-07-06 | 2016-01-21 | Novartis Ag | Norovirus derived immunogenic compositions and methods |
| EA030886B1 (ru) | 2010-08-17 | 2018-10-31 | Амбркс, Инк. | Модифицированные полипептиды релаксина, содержащие некодируемую в природе аминокислоту, связанную с полимером, и их применение |
| US9567386B2 (en) | 2010-08-17 | 2017-02-14 | Ambrx, Inc. | Therapeutic uses of modified relaxin polypeptides |
| TWI480288B (zh) | 2010-09-23 | 2015-04-11 | Lilly Co Eli | 牛顆粒細胞群落刺激因子及其變體之調配物 |
| DK3553177T3 (da) * | 2013-03-15 | 2021-02-15 | Univ Leland Stanford Junior | Benzylisoquinolinalkaloider (bia) som producerer mikrober, og fremgangsmåder til fremstilling og anvendelse deraf |
| CN114805532A (zh) | 2014-10-24 | 2022-07-29 | 百时美施贵宝公司 | 修饰的fgf-21多肽及其用途 |
| JP7266966B2 (ja) | 2015-05-08 | 2023-05-01 | ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー | エピメラーゼ及びベンジルイソキノリンアルカロイドを産生する方法 |
| PE20191716A1 (es) | 2017-02-08 | 2019-12-05 | Bristol Myers Squibb Co | Polipeptidos de relaxina modificada que comprenden un mejorador farmacocinetico y sus usos |
| CN120399912A (zh) | 2019-03-08 | 2025-08-01 | 小利兰·斯坦福大学托管委员会 | 产生莨菪烷生物碱(ta)的非植物宿主细胞及其制备和使用方法 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4880734A (en) * | 1984-05-11 | 1989-11-14 | Chiron Corporation | Eukaryotic regulatable transcription |
| CA1260858A (en) * | 1985-03-28 | 1989-09-26 | Lawrence S. Cousens | Expression using fused genes providing for protein product |
| EP0234871A3 (de) | 1986-02-28 | 1988-10-12 | Merck & Co. Inc. | Regulierbare Expression rekombinanter Proteine in Hefe |
| US5068185A (en) | 1986-07-10 | 1991-11-26 | Merck & Co., Inc. | Trains of yeast for the expression of heterologous genes |
-
1989
- 1989-04-21 CA CA000597461A patent/CA1324969C/en not_active Expired - Lifetime
- 1989-04-28 EP EP89304297A patent/EP0340986B1/de not_active Expired - Lifetime
- 1989-04-28 ES ES89304297T patent/ES2072900T3/es not_active Expired - Lifetime
- 1989-04-28 DE DE68922932T patent/DE68922932T2/de not_active Expired - Lifetime
- 1989-04-28 AT AT89304297T patent/ATE123521T1/de not_active IP Right Cessation
- 1989-05-02 JP JP01113585A patent/JP3074174B2/ja not_active Expired - Lifetime
- 1989-05-05 DK DK198902215A patent/DK175898B1/da not_active IP Right Cessation
-
1995
- 1995-03-01 US US08/397,553 patent/US6183985B1/en not_active Expired - Lifetime
-
2000
- 2000-01-12 JP JP2000004098A patent/JP3484386B2/ja not_active Expired - Lifetime
-
2002
- 2002-08-14 JP JP2002236635A patent/JP3561513B2/ja not_active Expired - Fee Related
-
2003
- 2003-12-10 JP JP2003412625A patent/JP2004081222A/ja not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| JPH02104291A (ja) | 1990-04-17 |
| DK175898B1 (da) | 2005-05-30 |
| JP3074174B2 (ja) | 2000-08-07 |
| CA1324969C (en) | 1993-12-07 |
| DE68922932D1 (de) | 1995-07-13 |
| EP0340986A2 (de) | 1989-11-08 |
| EP0340986A3 (de) | 1991-07-31 |
| EP0340986B1 (de) | 1995-06-07 |
| JP2000300277A (ja) | 2000-10-31 |
| DK221589D0 (da) | 1989-05-05 |
| JP2004081222A (ja) | 2004-03-18 |
| JP3561513B2 (ja) | 2004-09-02 |
| DE68922932T2 (de) | 1995-10-19 |
| ES2072900T3 (es) | 1995-08-01 |
| DK221589A (da) | 1989-11-07 |
| JP2003116525A (ja) | 2003-04-22 |
| US6183985B1 (en) | 2001-02-06 |
| JP3484386B2 (ja) | 2004-01-06 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification | ||
| EELA | Cancelled due to lapse of time |