ES2072900T3 - Expresion elevada de proteinas en levadura. - Google Patents

Expresion elevada de proteinas en levadura.

Info

Publication number
ES2072900T3
ES2072900T3 ES89304297T ES89304297T ES2072900T3 ES 2072900 T3 ES2072900 T3 ES 2072900T3 ES 89304297 T ES89304297 T ES 89304297T ES 89304297 T ES89304297 T ES 89304297T ES 2072900 T3 ES2072900 T3 ES 2072900T3
Authority
ES
Spain
Prior art keywords
yeast
high expression
yeast proteins
guest
refers
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES89304297T
Other languages
English (en)
Inventor
Jeffrey R Shuster
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis Vaccines and Diagnostics Inc
Original Assignee
Chiron Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chiron Corp filed Critical Chiron Corp
Application granted granted Critical
Publication of ES2072900T3 publication Critical patent/ES2072900T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/50—Fibroblast growth factor [FGF]
    • C07K14/503—Fibroblast growth factor [FGF] basic FGF [bFGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

LA INVENCION SE REFIERE A UN METODO PARA EXPRESAR PROTEINAS NO DE LEVADURAS BAJO EL CONTROL DE SECUENCIAS REGULADORAS DE ADH2 EN UNA LEVADURA HUESPED QUE TIENE EXPRESION MEJORADA DE ADRI. TAMBIEN SE REFIERE LA INVENCION A LEVADURAS HUESPED TRANSFORMADAS.
ES89304297T 1988-05-06 1989-04-28 Expresion elevada de proteinas en levadura. Expired - Lifetime ES2072900T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US19086888A 1988-05-06 1988-05-06

Publications (1)

Publication Number Publication Date
ES2072900T3 true ES2072900T3 (es) 1995-08-01

Family

ID=22703135

Family Applications (1)

Application Number Title Priority Date Filing Date
ES89304297T Expired - Lifetime ES2072900T3 (es) 1988-05-06 1989-04-28 Expresion elevada de proteinas en levadura.

Country Status (8)

Country Link
US (1) US6183985B1 (es)
EP (1) EP0340986B1 (es)
JP (4) JP3074174B2 (es)
AT (1) ATE123521T1 (es)
CA (1) CA1324969C (es)
DE (1) DE68922932T2 (es)
DK (1) DK175898B1 (es)
ES (1) ES2072900T3 (es)

Families Citing this family (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5981227A (en) * 1995-02-09 1999-11-09 Movartis Ag Process for the production of proteins
DE19544233A1 (de) * 1995-11-28 1997-06-05 Hoechst Ag Verfahren zur Nutzung des Hefe-ADH II-Promotorsystems zur biotechnologischen Produktion heterologer Proteine in hohen Ausbeuten
BR0212649A (pt) * 2001-09-20 2004-08-24 Toyota Motor Co Ltd Sistema para alcançar elevada expressão gênica
AU2005319099B2 (en) * 2004-02-02 2010-09-16 Ambrx, Inc. Modified human growth hormone
CN103755800B (zh) 2004-02-02 2016-02-24 Ambrx公司 经修饰的人类生长激素多肽与其用途
CN102532321B (zh) 2004-06-18 2018-10-12 Ambrx公司 新颖抗原结合多肽和其用途
US7638299B2 (en) * 2004-07-21 2009-12-29 Ambrx, Inc. Biosynthetic polypeptides utilizing non-naturally encoded amino acids
AU2005323106B2 (en) 2004-12-22 2010-07-29 Ambrx, Inc. Methods for expression and purification of recombinant human growth hormone
US7385028B2 (en) 2004-12-22 2008-06-10 Ambrx, Inc Derivatization of non-natural amino acids and polypeptides
KR101224781B1 (ko) 2004-12-22 2013-01-21 암브룩스, 인코포레이티드 비천연적으로 코딩된 아미노산을 포함하는 인간 성장호르몬의 조제물
NZ555425A (en) * 2004-12-22 2010-04-30 Ambrx Inc Compositions of aminoacyl-tRNA synthetase and uses thereof
WO2006133088A2 (en) * 2005-06-03 2006-12-14 Ambrx, Inc. Improved human interferon molecules and their uses
DE102005029170A1 (de) * 2005-06-23 2006-12-28 Consortium für elektrochemische Industrie GmbH System zur Genexpression in Hefen der Gattung Sporidiobolus
CA2618878C (en) 2005-08-18 2016-06-28 Ambrx, Inc. Compositions of trna and uses thereof
SI1954710T1 (sl) * 2005-11-08 2011-08-31 Ambrx Inc Pospeĺ evala za modifikacijo nenaravnih aminokislin in nenaravnih peptidov aminokislin
EP1951890A4 (en) 2005-11-16 2009-06-24 Ambrx Inc PROCESSES AND COMPOSITIONS WITH NON-NATURAL AMINO ACIDS
AU2006335256B2 (en) 2005-11-22 2012-10-18 Novartis Vaccines And Diagnostics, Inc. Norovirus and sapovirus antigens
WO2008030558A2 (en) * 2006-09-08 2008-03-13 Ambrx, Inc. Modified human plasma polypeptide or fc scaffolds and their uses
PT2064333E (pt) * 2006-09-08 2014-06-09 Ambrx Inc Supressor híbrido arnt para células de vertebrados
ES2457527T3 (es) * 2006-09-08 2014-04-28 Ambrx, Inc. ARNt Supresor hibrido en células de vertebrados
DK2106447T3 (da) 2007-01-26 2013-06-24 Novozymes As Fremgangsmåde til methanoluafhængig induktion fra methanolinducerbare promotorer i Pichia
KR101476472B1 (ko) 2007-03-30 2015-01-05 암브룩스, 인코포레이티드 변형된 ｆｇｆ-21 폴리펩티드 및 그 용도
KR20100019467A (ko) 2007-05-02 2010-02-18 암브룩스, 인코포레이티드 변형된 인터페론 베타 폴리펩티드 및 그의 용도
ES2632504T3 (es) 2007-11-20 2017-09-13 Ambrx, Inc. Polipéptidos de insulina modificados y sus usos
NZ602170A (en) 2008-02-08 2014-03-28 Ambrx Inc Modified leptin polypeptides and their uses
CN102105589A (zh) * 2008-07-11 2011-06-22 诺维信公司 巴斯德毕赤酵母(Pichia pastoris) DAS启动子变体
MX2011000859A (es) 2008-07-23 2011-02-24 Ambrx Inc Polipeptidos g-csf bovinos modificados y sus usos.
PL2342223T3 (pl) 2008-09-26 2017-09-29 Ambrx, Inc. Zmodyfikowane polipeptydy zwierzęcej erytropoetyny i ich zastosowania
NO2337846T3 (es) 2008-09-26 2018-06-16
NZ600361A (en) 2009-12-21 2014-06-27 Ambrx Inc Modified bovine somatotropin polypeptides and their uses
EP2516455A4 (en) 2009-12-21 2013-05-22 Ambrx Inc MODIFIED SWINE SOMATOTROPIN POLYPEPTIDES AND THEIR USES
CA2804501C (en) 2010-07-06 2021-01-26 Novartis Ag Noro virus derived immunogenic compositions and methods
SMT201900495T1 (it) 2010-08-17 2019-11-13 Ambrx Inc Polipeptidi di relaxina modificati e loro usi
US9567386B2 (en) 2010-08-17 2017-02-14 Ambrx, Inc. Therapeutic uses of modified relaxin polypeptides
TWI480288B (zh) 2010-09-23 2015-04-11 Lilly Co Eli 牛顆粒細胞群落刺激因子及其變體之調配物
EP3865580A1 (en) * 2013-03-15 2021-08-18 The Board of Trustees of the Leland Stanford Junior University Benzylisoquinoline alkaloids (bia) producing microbes, and methods of making and using the same
BR112017008157A2 (pt) 2014-10-24 2017-12-19 Bristol Myers Squibb Co polipeptídeos de fgf-21 modificados e usos dos mesmos
ES2945359T3 (es) 2015-05-08 2023-06-30 Univ Leland Stanford Junior Métodos para producir epimerasas y alcaloides de bencilisoquinolina
US10266578B2 (en) 2017-02-08 2019-04-23 Bristol-Myers Squibb Company Modified relaxin polypeptides comprising a pharmacokinetic enhancer and uses thereof
WO2020185626A1 (en) 2019-03-08 2020-09-17 The Board Of Trustees Of The Leland Stanford Junior University Tropane alkaloid (ta) producing non-plant host cells, and methods of making and using the same

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4880734A (en) * 1984-05-11 1989-11-14 Chiron Corporation Eukaryotic regulatable transcription
CA1260858A (en) * 1985-03-28 1989-09-26 Lawrence S. Cousens Expression using fused genes providing for protein product
EP0234871A3 (en) 1986-02-28 1988-10-12 Merck & Co. Inc. Regulatable expression of recombinant proteins in yeast
US5068185A (en) 1986-07-10 1991-11-26 Merck & Co., Inc. Trains of yeast for the expression of heterologous genes

Also Published As

Publication number Publication date
JP3074174B2 (ja) 2000-08-07
US6183985B1 (en) 2001-02-06
JP3561513B2 (ja) 2004-09-02
DE68922932T2 (de) 1995-10-19
JP2003116525A (ja) 2003-04-22
DE68922932D1 (de) 1995-07-13
EP0340986A2 (en) 1989-11-08
DK221589A (da) 1989-11-07
EP0340986A3 (en) 1991-07-31
EP0340986B1 (en) 1995-06-07
DK221589D0 (da) 1989-05-05
JP3484386B2 (ja) 2004-01-06
JP2000300277A (ja) 2000-10-31
DK175898B1 (da) 2005-05-30
JP2004081222A (ja) 2004-03-18
JPH02104291A (ja) 1990-04-17
CA1324969C (en) 1993-12-07
ATE123521T1 (de) 1995-06-15

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