ATE160587T1 - Expression rekombinanter polypeptide mit verbesserter möglichkeit zur reinigung - Google Patents

Expression rekombinanter polypeptide mit verbesserter möglichkeit zur reinigung

Info

Publication number
ATE160587T1
ATE160587T1 AT91915080T AT91915080T ATE160587T1 AT E160587 T1 ATE160587 T1 AT E160587T1 AT 91915080 T AT91915080 T AT 91915080T AT 91915080 T AT91915080 T AT 91915080T AT E160587 T1 ATE160587 T1 AT E160587T1
Authority
AT
Austria
Prior art keywords
purification
expression
recombinant polypeptides
improved capability
carrier
Prior art date
Application number
AT91915080T
Other languages
English (en)
Inventor
S Joseph Tarnowski
Sandra Hilliker
W Scott Willett
Original Assignee
Scios Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Scios Inc filed Critical Scios Inc
Application granted granted Critical
Publication of ATE160587T1 publication Critical patent/ATE160587T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/58—Atrial natriuretic factor complex; Atriopeptin; Atrial natriuretic peptide [ANP]; Cardionatrin; Cardiodilatin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/61—Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Plant Pathology (AREA)
  • Endocrinology (AREA)
  • Microbiology (AREA)
  • Cardiology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AT91915080T 1990-08-07 1991-08-07 Expression rekombinanter polypeptide mit verbesserter möglichkeit zur reinigung ATE160587T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/564,259 US5202239A (en) 1990-08-07 1990-08-07 Expression of recombinant polypeptides with improved purification

Publications (1)

Publication Number Publication Date
ATE160587T1 true ATE160587T1 (de) 1997-12-15

Family

ID=24253763

Family Applications (1)

Application Number Title Priority Date Filing Date
AT91915080T ATE160587T1 (de) 1990-08-07 1991-08-07 Expression rekombinanter polypeptide mit verbesserter möglichkeit zur reinigung

Country Status (10)

Country Link
US (2) US5202239A (de)
EP (1) EP0542863B1 (de)
JP (1) JP3369168B2 (de)
AT (1) ATE160587T1 (de)
CA (1) CA2089094C (de)
DE (1) DE69128285T2 (de)
DK (1) DK0542863T3 (de)
ES (1) ES2114539T3 (de)
GR (1) GR3026230T3 (de)
WO (1) WO1992002550A1 (de)

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FR2686899B1 (fr) * 1992-01-31 1995-09-01 Rhone Poulenc Rorer Sa Nouveaux polypeptides biologiquement actifs, leur preparation et compositions pharmaceutiques les contenant.
AU6360394A (en) * 1993-03-03 1994-09-26 Mayo Foundation For Medical Education And Research Vasonatrin peptide and analogs thereof
DK144093D0 (de) * 1993-12-23 1993-12-23 Novo Nordisk As
US6069230A (en) * 1994-11-10 2000-05-30 Promega Corporation High level expression and facile purification of proteins, peptides and conjugates for immunization, purification and detection applications
US6764689B1 (en) 1994-11-10 2004-07-20 Promega Corporation High level expression and facile purification of proteins, peptides and conjugates for immunization, purification and detection applications
US5935824A (en) * 1996-01-31 1999-08-10 Technologene, Inc. Protein expression system
US6277819B1 (en) * 1996-08-30 2001-08-21 Eli Lilly And Company Use of GLP-1 or analogs in treatment of myocardial infarction
US5968782A (en) * 1996-12-04 1999-10-19 Brigham And Womens's Hospital, Inc. Mast cell protease that cleaves fibrinogen
US5955431A (en) * 1997-02-05 1999-09-21 Brigham And Women's Hospital, Inc. Mast cell protease peptide inhibitors
US6077689A (en) * 1997-12-24 2000-06-20 Amgen Inc. Enhanced solubility of recombinant proteins
EP1090132A1 (de) * 1998-06-26 2001-04-11 PPL Therapeutics (Scotland) Limited Verfahren zur herstellung von rekombinanten polypetide
CN1242062C (zh) * 1998-07-10 2006-02-15 赛欧斯公司 生产等电点高于8或低于5的多肽的方法
US6461834B1 (en) 1998-11-06 2002-10-08 Bionebraska, Inc. Clostripain catalyzed amidation of peptides
US6082594A (en) * 1998-12-31 2000-07-04 Snowmaker Productions, Inc. Snowfall simulator with agitating dispenser
WO2000071576A2 (en) * 1999-05-24 2000-11-30 Mayo Foundation For Medical Education And Research Adenovirus vectors encoding brain natriuretic peptide
US20060160740A1 (en) * 1999-10-21 2006-07-20 Suad Efendic Use of GLP-1 or analogs in treatment of stroke
US6407211B1 (en) * 1999-12-17 2002-06-18 Mayo Foundation For Medical Education And Research Chimeric natriuretic peptides
US6926898B2 (en) * 2000-04-12 2005-08-09 Human Genome Sciences, Inc. Albumin fusion proteins
US20050100991A1 (en) * 2001-04-12 2005-05-12 Human Genome Sciences, Inc. Albumin fusion proteins
US6469145B1 (en) * 2000-06-26 2002-10-22 The United States Of America As Represented By The Secretary Of The Army One-step purification process for organophosphorus hydrolase enzyme
US6632638B1 (en) 2000-11-17 2003-10-14 Amgen, Inc. Enhanced solubility of recombinant proteins using Uracil DNA glycosylase inhibitor
WO2002097038A2 (en) * 2001-05-25 2002-12-05 Human Genome Sciences, Inc. Chemokine beta-1 fusion proteins
CN100427506C (zh) * 2001-07-26 2008-10-22 高级蛋白质技术公司 从融合多肽制备感兴趣的多肽的方法
CA2841097A1 (en) 2001-12-21 2003-07-24 Human Genome Sciences, Inc. Albumin and g-csf fusion proteins
WO2005003296A2 (en) 2003-01-22 2005-01-13 Human Genome Sciences, Inc. Albumin fusion proteins
WO2003059934A2 (en) * 2001-12-21 2003-07-24 Human Genome Sciences, Inc. Albumin fusion proteins
US20030134352A1 (en) * 2002-01-04 2003-07-17 Freimuth Paul I. Facilitating protein folding and solubility by use of peptide extensions
US7229554B2 (en) * 2002-09-25 2007-06-12 Novo Nordisk A/S Purification process comprising microfiltration at elevated temperatures
SG159387A1 (en) 2002-11-26 2010-03-30 Biocon Ltd In Modified natriuretic compounds, conjugates, and uses thereof
CN102816241B (zh) * 2004-02-09 2015-07-22 人类基因科学公司 清蛋白融合蛋白
US8137929B2 (en) 2005-04-15 2012-03-20 Novo Nordisk Health Care Ag Basic protein purification tags from thermophilic bacteria
US7334279B2 (en) * 2005-11-09 2008-02-26 Oprandi Arthur V Mattress pad
AU2007233123A1 (en) 2006-03-30 2007-10-11 Palatin Technologies, Inc. Linear natriuretic peptide constructs
AU2007233116B2 (en) 2006-03-30 2013-05-09 Palatin Technologies, Inc. Cyclic natriuretic peptide constructs
US8580746B2 (en) 2006-03-30 2013-11-12 Palatin Technologies, Inc. Amide linkage cyclic natriuretic peptide constructs
CN101563358A (zh) * 2006-10-13 2009-10-21 诺沃-诺迪斯克保健股份有限公司 融合于碱性蛋白标签的加工酶
EP2678002A2 (de) 2011-02-25 2014-01-01 Medtronic, Inc. Therapie für nierenerkrankungen und/oder herzinsuffizienz
CN103906761B (zh) 2011-08-30 2016-12-21 梅约医学教育与研究基金会 利钠多肽
EP2750697A4 (de) 2011-09-02 2015-03-25 Medtronic Inc Chimäre natriuretische peptidzusammensetzungen und verfahren zu ihrer herstellung
WO2013103896A1 (en) 2012-01-06 2013-07-11 Mayo Foundation For Medical Education And Research Treating cardiovascular or renal diseases
US9163263B2 (en) 2012-05-02 2015-10-20 The Goodyear Tire & Rubber Company Identification of isoprene synthase variants with improved properties for the production of isoprene

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4431739A (en) * 1979-11-05 1984-02-14 Genentech, Inc. Transformant bacterial culture capable of expressing heterologous protein
US4880911A (en) * 1982-03-19 1989-11-14 G. D. Searle & Co. Fused polypeptides and methods for their detection
US4532207A (en) * 1982-03-19 1985-07-30 G. D. Searle & Co. Process for the preparation of polypeptides utilizing a charged amino acid polymer and exopeptidase
US4897348A (en) * 1983-08-25 1990-01-30 Sri International Recombinant materials and methods for producing human connective tissue-activating peptide-III and analogs thereof
US4618600A (en) * 1984-04-19 1986-10-21 Biotechnology Research Associates, J.V. Novel polypeptide diuretic/vasodilators
CA1213537A (en) * 1984-05-01 1986-11-04 Canadian Patents And Development Limited - Societe Canadienne Des Brevets Et D'exploitation Limitee Polypeptide expression method
EP0207044A1 (de) * 1985-06-20 1986-12-30 Monsanto Company Freisetzung von Peptiden aus Polypeptiden
GB8518753D0 (en) * 1985-07-24 1985-08-29 Glaxo Group Ltd Microbiological products
US4743679A (en) * 1986-02-24 1988-05-10 Creative Biomolecules, Inc. Process for producing human epidermal growth factor and analogs thereof
DK175535B1 (da) * 1987-03-04 2004-11-29 Daiichi Suntory Pharma Co Ltd Fremgangsmåde til fremstilling af et fysiologisk aktivt cysteinholdigt peptid

Also Published As

Publication number Publication date
DE69128285T2 (de) 1998-06-25
WO1992002550A1 (en) 1992-02-20
EP0542863A1 (de) 1993-05-26
JPH06504036A (ja) 1994-05-12
US5322930A (en) 1994-06-21
US5202239A (en) 1993-04-13
GR3026230T3 (en) 1998-05-29
ES2114539T3 (es) 1998-06-01
DE69128285D1 (de) 1998-01-08
CA2089094A1 (en) 1992-02-08
CA2089094C (en) 2002-02-05
EP0542863B1 (de) 1997-11-26
DK0542863T3 (da) 1998-08-10
JP3369168B2 (ja) 2003-01-20
EP0542863A4 (en) 1993-09-22

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Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
EELA Cancelled due to lapse of time