ATE161044T1 - Methode zur herstellung von sekretierbaren glycosyltransferasen - Google Patents

Methode zur herstellung von sekretierbaren glycosyltransferasen

Info

Publication number
ATE161044T1
ATE161044T1 AT90916656T AT90916656T ATE161044T1 AT E161044 T1 ATE161044 T1 AT E161044T1 AT 90916656 T AT90916656 T AT 90916656T AT 90916656 T AT90916656 T AT 90916656T AT E161044 T1 ATE161044 T1 AT E161044T1
Authority
AT
Austria
Prior art keywords
soluble
secretal
producing
secretable
glycosyltransferases
Prior art date
Application number
AT90916656T
Other languages
English (en)
Inventor
James C Paulson
Eryn Ujita-Lee
Karen J Colley
Beverly Adler
Jeffrey K Browne
Jasminder Weinstein
Original Assignee
Univ California
Amgen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ California, Amgen Inc filed Critical Univ California
Application granted granted Critical
Publication of ATE161044T1 publication Critical patent/ATE161044T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1048Glycosyltransferases (2.4)
    • C12N9/1051Hexosyltransferases (2.4.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/18Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/26Preparation of nitrogen-containing carbohydrates

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
  • Artificial Filaments (AREA)
  • Inorganic Fibers (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
AT90916656T 1989-10-24 1990-10-24 Methode zur herstellung von sekretierbaren glycosyltransferasen ATE161044T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/426,577 US5032519A (en) 1989-10-24 1989-10-24 Method for producing secretable glycosyltransferases and other Golgi processing enzymes

Publications (1)

Publication Number Publication Date
ATE161044T1 true ATE161044T1 (de) 1997-12-15

Family

ID=23691361

Family Applications (1)

Application Number Title Priority Date Filing Date
AT90916656T ATE161044T1 (de) 1989-10-24 1990-10-24 Methode zur herstellung von sekretierbaren glycosyltransferasen

Country Status (11)

Country Link
US (1) US5032519A (de)
EP (1) EP0497878B1 (de)
JP (1) JP3121342B2 (de)
AT (1) ATE161044T1 (de)
AU (1) AU646233B2 (de)
CA (1) CA2065839C (de)
DE (1) DE69031805T2 (de)
DK (1) DK0497878T3 (de)
ES (1) ES2112254T3 (de)
GR (1) GR3026217T3 (de)
WO (1) WO1991006635A1 (de)

Families Citing this family (86)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3203599B2 (ja) * 1989-10-24 2001-08-27 カイロン コーポレイション 感染性タンパク質デリバリーシステム
US5541083A (en) * 1989-10-24 1996-07-30 The Regents Of The University Of California Method for producing secretable glycosyltransferases and other golgi processing enzymes
KR920702721A (ko) * 1990-04-16 1992-10-06 원본미기재 사카라이드 조성물, 그의 합성 방법 및 장치
US5589372A (en) * 1990-09-26 1996-12-31 E. R. Squibb & Sons, Inc. Squalene synthetase
US5475101A (en) * 1990-10-05 1995-12-12 Genencor International, Inc. DNA sequence encoding endoglucanase III cellulase
US5401650A (en) * 1990-10-24 1995-03-28 The Mount Sinai School Of Medicine Of The City University Of New York Cloning and expression of biologically active α-galactosidase A
GB2256197B (en) * 1991-05-31 1995-11-22 Ciba Geigy Ag Yeast as host for expression of heterologous glycosyl transferase enzymes
SE9201544L (sv) * 1991-05-31 1992-12-01 Ciba Geigy Ag Saett att framstaella glykosyltransferaser
US5374655A (en) * 1991-06-10 1994-12-20 Alberta Research Council Methods for the synthesis of monofucosylated oligosaccharides terminating in di-N-acetyllactosaminyl structures
US5593857A (en) * 1991-08-23 1997-01-14 Scios Inc. Production of homogeneous truncated CNTF
US5384249A (en) * 1991-12-17 1995-01-24 Kyowa Hakko Kogyo Co., Ltd. α2→3 sialyltransferase
US5858751A (en) * 1992-03-09 1999-01-12 The Regents Of The University Of California Compositions and methods for producing sialyltransferases
HU219260B (en) * 1992-03-09 2001-03-28 Cytel Corp Recombinant polypeptid with sialyltransferase activity, dna molecule coding thereof and vectors
US5541095A (en) * 1992-06-16 1996-07-30 University Of Massachusetts Medical Center Glycosaminoglycan specific sulfotransferases
US5602003A (en) * 1992-06-29 1997-02-11 University Of Georgia Research Foundation N-acetylglucosaminyltransferase V gene
KR950704497A (ko) * 1992-11-27 1995-11-20 베르너 발데크 글리코실트랜스퍼라제 활성을 가진 단백질(Proteins Having Glycosyltransferase Activity)
JPH09501044A (ja) * 1993-05-14 1997-02-04 ジ・アップジョン・カンパニー UDP−GALNAc:ポリペプチド、N−アセチルガラクトサミニルトランスフェラーゼをコードするクローン化されたDNA
CN1129953A (zh) * 1993-07-15 1996-08-28 尼奥斯药品公司 糖组分的合成方法
US5484590A (en) * 1993-09-09 1996-01-16 La Jolla Cancer Research Foundation Expression of the developmental I antigen by a cloned human cDNA encoding a member of a β-1,6-N-acetylglucosaminyltransferase gene family
US6485930B1 (en) * 1993-09-15 2002-11-26 The Scripps Research Institute Mannosyl transfer with regeneration of GDP-mannose
WO1995024494A1 (en) * 1994-03-09 1995-09-14 Abbott Laboratories Humanized milk
AU692841B2 (en) * 1994-03-09 1998-06-18 Abbott Laboratories Transgenic animals producing oligosaccharides and glycoconjugates
US6204431B1 (en) 1994-03-09 2001-03-20 Abbott Laboratories Transgenic non-human mammals expressing heterologous glycosyltransferase DNA sequences produce oligosaccharides and glycoproteins in their milk
US5750176A (en) * 1994-03-09 1998-05-12 Abbott Laboratories Transgenic non-human mammal milk comprising 2'-fucosyl-lactose
US5965457A (en) * 1995-06-06 1999-10-12 Magnani; John L. Methods of screening for a candidate compound able to bind to CD34+ cells
US5910570A (en) * 1995-11-13 1999-06-08 Pharmacia & Upjohn Company Cloned DNA encoding a UDP-GalNAc: polypeptide N-acetylgalactosaminy-ltransferase
US6339048B1 (en) * 1999-12-23 2002-01-15 Elementis Specialties, Inc. Oil and oil invert emulsion drilling fluids with improved anti-settling properties
US7244601B2 (en) * 1997-12-15 2007-07-17 National Research Council Of Canada Fusion proteins for use in enzymatic synthesis of oligosaccharides
US6207414B1 (en) 1998-01-29 2001-03-27 The Board Of Regents Of The University Of Oklahoma Tyrosylprotein sulfotransferases and methods of use thereof
US6713283B2 (en) 1998-01-29 2004-03-30 The Board Of Regents Of The University Of Oklahoma Tyrosylprotein sulfotransferases
US6207432B1 (en) 1998-01-29 2001-03-27 The Board Of Regents Of The University Of Oklahoma Tyrosylprotein sulfotransferases and methods of use thereof
US6605455B1 (en) 1998-01-29 2003-08-12 The Board Of Regsnts Of The University Of Oklahoma Tyrosylprotein sulfotransferases and methods of use thereof
US6204016B1 (en) 1998-01-29 2001-03-20 The Board Of Regents Of The University Of Oklahoma Tyrosylprotein sulfotransferases and methods of use thereof
US6060295A (en) * 1998-01-29 2000-05-09 The Board Of Regents Of The University Of Oklahoma Nucleic acids and expression systems encoding tyrosylprotein sulfo transferases
US6071732A (en) * 1998-01-29 2000-06-06 The Board Of Regents Of The University Of Oklahoma Tyrosylprotein sulfotransferases, nucleic acids encoding tyrosylprotein sulfotransferases, and methods of use thereof
US7029890B2 (en) * 1998-01-29 2006-04-18 Siwa Biotech Corporation Tyrosylprotein sulfotransferases and methods of use thereof
US6994966B2 (en) * 2000-02-17 2006-02-07 Glycominds Ltd. Combinatorial complex carbohydrate libraries and methods for the manufacture and uses thereof
US20010051349A1 (en) 2000-02-17 2001-12-13 Glycominds Ltd. Combinatorial complex carbohydrate libraries and methods for the manufacture and uses thereof
AUPQ275799A0 (en) * 1999-09-10 1999-10-07 Luminis Pty Limited Recombinant bacterium expressing an oligosaccharide receptor mimic
JP2004528001A (ja) * 2000-05-12 2004-09-16 ネオーズ テクノロジーズ, インコーポレイテッド インビトロにおけるフコシル化組換えグリコペプチド
US7863020B2 (en) * 2000-06-28 2011-01-04 Glycofi, Inc. Production of sialylated N-glycans in lower eukaryotes
US7449308B2 (en) * 2000-06-28 2008-11-11 Glycofi, Inc. Combinatorial DNA library for producing modified N-glycans in lower eukaryotes
US7625756B2 (en) 2000-06-28 2009-12-01 GycoFi, Inc. Expression of class 2 mannosidase and class III mannosidase in lower eukaryotic cells
ES2252261T3 (es) * 2000-06-28 2006-05-16 Glycofi, Inc. Metodos para producir glicoproteinas modificadas.
US8697394B2 (en) * 2000-06-28 2014-04-15 Glycofi, Inc. Production of modified glycoproteins having multiple antennary structures
US7598055B2 (en) * 2000-06-28 2009-10-06 Glycofi, Inc. N-acetylglucosaminyltransferase III expression in lower eukaryotes
US7173003B2 (en) 2001-10-10 2007-02-06 Neose Technologies, Inc. Granulocyte colony stimulating factor: remodeling and glycoconjugation of G-CSF
US7214660B2 (en) * 2001-10-10 2007-05-08 Neose Technologies, Inc. Erythropoietin: remodeling and glycoconjugation of erythropoietin
US7157277B2 (en) * 2001-11-28 2007-01-02 Neose Technologies, Inc. Factor VIII remodeling and glycoconjugation of Factor VIII
US8008252B2 (en) 2001-10-10 2011-08-30 Novo Nordisk A/S Factor VII: remodeling and glycoconjugation of Factor VII
ES2411007T3 (es) 2001-10-10 2013-07-04 Novo Nordisk A/S Remodelación y glicoconjugación de péptidos
CN101613705A (zh) 2002-03-19 2009-12-30 国际植物研究所 在植物中优化聚糖生成
WO2003091402A2 (en) * 2002-04-23 2003-11-06 University Of Georgia Research Foundation, Inc. N-ACETYLGLUCOSAMINYLTRANSFERASE Vb CODING SEQUENCE, RECOMBINANT CELLS AND METHODS
DE60336555D1 (de) 2002-06-21 2011-05-12 Novo Nordisk Healthcare Ag Pegylierte glykoformen von faktor vii
AU2003301332B2 (en) * 2002-10-17 2008-06-12 Pharming Intellectual Property B.V. Protein modification
US7332299B2 (en) 2003-02-20 2008-02-19 Glycofi, Inc. Endomannosidases in the modification of glycoproteins in eukaryotes
NZ542094A (en) 2003-03-14 2008-12-24 Neose Technologies Inc Branched polymer conjugates comprising a peptide and water-soluble polymer chains
PL1615945T3 (pl) 2003-04-09 2012-03-30 Ratiopharm Gmbh Sposoby glikopegylacji i białka/peptydy wytwarzane tymi sposobami
US8791070B2 (en) 2003-04-09 2014-07-29 Novo Nordisk A/S Glycopegylated factor IX
JP2007523630A (ja) * 2003-05-09 2007-08-23 ネオス テクノロジーズ インコーポレイテッド ヒト成長ホルモングリコシル化突然変異体の組成と調合法
US9005625B2 (en) 2003-07-25 2015-04-14 Novo Nordisk A/S Antibody toxin conjugates
US20080305992A1 (en) 2003-11-24 2008-12-11 Neose Technologies, Inc. Glycopegylated erythropoietin
US8633157B2 (en) 2003-11-24 2014-01-21 Novo Nordisk A/S Glycopegylated erythropoietin
US20060040856A1 (en) * 2003-12-03 2006-02-23 Neose Technologies, Inc. Glycopegylated factor IX
NZ548123A (en) 2004-01-08 2010-05-28 Novo Nordisk As O-linked glycosylation of peptides
AU2005222782B2 (en) 2004-02-04 2010-06-17 Ratiopharm Gmbh Methods of refolding mammalian glycosyltransferases
US20080300173A1 (en) 2004-07-13 2008-12-04 Defrees Shawn Branched Peg Remodeling and Glycosylation of Glucagon-Like Peptides-1 [Glp-1]
US20090292110A1 (en) * 2004-07-23 2009-11-26 Defrees Shawn Enzymatic modification of glycopeptides
WO2006031811A2 (en) 2004-09-10 2006-03-23 Neose Technologies, Inc. Glycopegylated interferon alpha
WO2006050247A2 (en) 2004-10-29 2006-05-11 Neose Technologies, Inc. Remodeling and glycopegylation of fibroblast growth factor (fgf)
US9029331B2 (en) 2005-01-10 2015-05-12 Novo Nordisk A/S Glycopegylated granulocyte colony stimulating factor
WO2006121569A2 (en) 2005-04-08 2006-11-16 Neose Technologies, Inc. Compositions and methods for the preparation of protease resistant human growth hormone glycosylation mutants
EP2975135A1 (de) * 2005-05-25 2016-01-20 Novo Nordisk A/S Glykopegylierter Faktor IX
US20070105755A1 (en) * 2005-10-26 2007-05-10 Neose Technologies, Inc. One pot desialylation and glycopegylation of therapeutic peptides
MX2008002395A (es) * 2005-08-19 2008-03-18 Neose Technologies Inc Factor vii y factor viia glicopegilados.
WO2007056191A2 (en) 2005-11-03 2007-05-18 Neose Technologies, Inc. Nucleotide sugar purification using membranes
EP2049144B8 (de) 2006-07-21 2015-02-18 ratiopharm GmbH Glycosylierung von peptiden über o-verbundene glycosylierungssequenzen
EP2054521A4 (de) * 2006-10-03 2012-12-19 Novo Nordisk As Verfahren zur reinigung von polypeptid-konjugate
HRP20130382T1 (hr) 2007-04-03 2013-05-31 Biogenerix Ag Postupci lijeäśenja pomoä†u glikopegiliranog g-csf
CA2690611C (en) 2007-06-12 2015-12-08 Novo Nordisk A/S Improved process for the production of nucleotide sugars
US8207112B2 (en) 2007-08-29 2012-06-26 Biogenerix Ag Liquid formulation of G-CSF conjugate
PL2257311T3 (pl) 2008-02-27 2014-09-30 Novo Nordisk As Koniugaty cząsteczek czynnika VIII
EP2415779B1 (de) 2010-08-02 2015-01-21 ratiopharm GmbH Verfahren zur Herstellung und Reinigung einer aktiven löslichen Sialyltransferase
CA2908407C (en) 2013-05-29 2022-06-14 F. Hoffmann-La Roche Ag Quantitative control of sialylation
EP3017057B1 (de) 2013-07-05 2019-10-02 F.Hoffmann-La Roche Ag Verfahren zur mono- und bi-sialylierung von glykoproteinen unter verwendung von n-terminal verkürzten mutanten der beta-galaktosid alpha-2,6-sialyltransferase
EP2821482A1 (de) 2013-07-05 2015-01-07 Roche Diagniostics GmbH N-terminal verkürzte Glykosyltransferasen

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2228127A (en) * 1988-12-09 1990-08-15 Qudos Sa Display device
WO1990007000A2 (en) * 1988-12-13 1990-06-28 La Jolla Cancer Research Foundation Nucleotides encoding human b1, 4-galactosyltransferase and uses thereof

Also Published As

Publication number Publication date
DK0497878T3 (da) 1998-01-12
GR3026217T3 (en) 1998-05-29
JP3121342B2 (ja) 2000-12-25
CA2065839A1 (en) 1991-04-25
JPH05504678A (ja) 1993-07-22
DE69031805T2 (de) 1998-04-23
DE69031805D1 (de) 1998-01-22
EP0497878A1 (de) 1992-08-12
EP0497878B1 (de) 1997-12-10
EP0497878A4 (en) 1993-01-07
US5032519A (en) 1991-07-16
CA2065839C (en) 2000-01-11
AU6648090A (en) 1991-05-31
AU646233B2 (en) 1994-02-17
ES2112254T3 (es) 1998-04-01
WO1991006635A1 (en) 1991-05-16

Similar Documents

Publication Publication Date Title
ATE161044T1 (de) Methode zur herstellung von sekretierbaren glycosyltransferasen
ATE527367T1 (de) Herstellung von oligosacchariden in transgenen pflanzen
ATE466094T1 (de) Verfahren zur herstellung von oligosacchariden
ES8202362A1 (es) Procedimiento para preparar un acido desoxi-ribonucleico (adn) recombinante
MXPA04006357A (es) Metodos para disenar estructuras de carbohidrato del tipo de mamifero.
DE69939947D1 (de) Verfahren zur herstellung von polypeptiden in mutierten aspergillus zellen
ATE290068T1 (de) Beta 1-4 n-acetylglucosaminyltransferase sowie das für diese kodierende gen
ATE246251T1 (de) Verfahren zur herstellung von polypeptiden in surfaktinmutanten von bacillus zellen
AU4940096A (en) Host cell expressing reduced levels of a metalloprotease and methods using the host cell in protein production
DE68925097D1 (de) Biokatalysatoren
ATE410054T1 (de) Durch elektroporation vermittelter molekulartransfer in intakten pflanzen
ATE92109T1 (de) Verfahren zur herstellung von l-rhamnose.
DK0990704T3 (da) Ikke-reducerende sacchariddannende enzym, trehalosefrigivende enzym og fremgangsmåde til fremstilling af saccharider under anvendelse af enzymerne
NZ208298A (en) Process of producing an "alpha-amylase" by cultivation of clostridium strains
ATE123304T1 (de) Durch rekombinante dns-technologie konstruierte amylolytische enzymproduzierende mikroorganismen und ihre verwendung in gärungsverfahren.
WO2000046375A3 (en) Methods for producing polypeptides in fungal cells
DK0504279T3 (da) Fermenteringsfremgangsmåde til fremstilling af pyrimidindeoxyribonukleosider
DE69112415D1 (de) Verfahren zur herstellung von glukose und fruktose aus sukrose.
DK63681A (da) Katalysatorer til fremstilling og omdannelse af naturlige produkter som har deres oprindelse i hoejere planter fremgangsmaade til fremstilling af katalusatorerne og deres anvendelse
ES521943A0 (es) Un procedimiento de produccion de glucosa deshidrogenasa.
Paulson et al. Method for producing secretable glycosyltransferases and other golgi processing enzymes
DE69834738D1 (de) Expressionssystem
KR960001119A (ko) 표고버섯과 영지버섯의 원형질체 융합체및 이의 제조방법
ES2026319A6 (es) Procedimiento de produccion de hidantoinasa mediante la utilizacion de una nueva cepa de streptomyces sp.y-183.
RU92009231A (ru) Способ получения супероксиддисмутазы

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
EELA Cancelled due to lapse of time