ATE164184T1 - Expression von menschlichen serum-albuminen in pichia pastoris - Google Patents

Expression von menschlichen serum-albuminen in pichia pastoris

Info

Publication number
ATE164184T1
ATE164184T1 AT92107043T AT92107043T ATE164184T1 AT E164184 T1 ATE164184 T1 AT E164184T1 AT 92107043 T AT92107043 T AT 92107043T AT 92107043 T AT92107043 T AT 92107043T AT E164184 T1 ATE164184 T1 AT E164184T1
Authority
AT
Austria
Prior art keywords
pichia pastoris
expression
human serum
serum albumins
hsa
Prior art date
Application number
AT92107043T
Other languages
English (en)
Inventor
William Dudley Prevatt
Kotikanyad Sreekrishna
Original Assignee
Res Corp Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Res Corp Technologies Inc filed Critical Res Corp Technologies Inc
Application granted granted Critical
Publication of ATE164184T1 publication Critical patent/ATE164184T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/76—Albumins
    • C07K14/765—Serum albumin, e.g. HSA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/32—Processes using, or culture media containing, lower alkanols, i.e. C1 to C6
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Toxicology (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
AT92107043T 1991-04-26 1992-04-24 Expression von menschlichen serum-albuminen in pichia pastoris ATE164184T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/691,079 US5330901A (en) 1991-04-26 1991-04-26 Expression of human serum albumin in Pichia pastoris

Publications (1)

Publication Number Publication Date
ATE164184T1 true ATE164184T1 (de) 1998-04-15

Family

ID=24775069

Family Applications (1)

Application Number Title Priority Date Filing Date
AT92107043T ATE164184T1 (de) 1991-04-26 1992-04-24 Expression von menschlichen serum-albuminen in pichia pastoris

Country Status (8)

Country Link
US (1) US5330901A (de)
EP (1) EP0510678B1 (de)
JP (2) JP3579430B2 (de)
AT (1) ATE164184T1 (de)
CA (1) CA2059167C (de)
DE (1) DE69224768T2 (de)
DK (1) DK0510678T3 (de)
ES (1) ES2113387T3 (de)

Families Citing this family (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0669365B2 (ja) * 1989-05-22 1994-09-07 株式会社ミドリ十字 アルブミン遺伝子を含むプラスミド、形質転換体、形質転換体の製造方法、アルブミンの製造方法
FR2686620B1 (fr) * 1992-01-27 1995-06-23 Rhone Poulenc Rorer Sa Serum-albumine humaine, preparation et utilisation.
FR2686899B1 (fr) 1992-01-31 1995-09-01 Rhone Poulenc Rorer Sa Nouveaux polypeptides biologiquement actifs, leur preparation et compositions pharmaceutiques les contenant.
JP3360315B2 (ja) * 1992-07-31 2002-12-24 三菱ウェルファーマ株式会社 ヒト血清アルブミンの高度精製方法
US5728553A (en) * 1992-09-23 1998-03-17 Delta Biotechnology Limited High purity albumin and method of producing
EP0776369A4 (de) * 1993-11-16 2000-11-22 Lilly Co Eli Fuer die pankreas - carboxypeptidan - b aus schweinen kodierende dna - sequenzen
CA2136564C (en) * 1993-11-26 2008-04-08 Kaoru Kobayashi Process for producing human serum albumin
JPH07308199A (ja) * 1994-05-18 1995-11-28 Green Cross Corp:The ヒト血清アルブミンの製造方法
US5863894A (en) * 1995-06-05 1999-01-26 Corvas International, Inc. Nematode-extracted anticoagulant protein
ES2276396T3 (es) 1994-10-18 2007-06-16 Dendreon Corporation Proteinas anticoagulantes e inhibidores de la serina proteasa extraidos de nematodos.
US5866543A (en) * 1995-06-05 1999-02-02 Corvas International, Inc. Nematode-extracted anticoagulant protein
US5945275A (en) * 1994-10-18 1999-08-31 Corvas International, Inc. Nematode-extracted anticoagulant protein
US5866542A (en) * 1994-10-18 1999-02-02 Corvas International, Inc. Nematode-extracted anticoagulant protein
CA2202351A1 (en) * 1994-10-18 1996-04-25 George Phillip Vlasuk Nematode-extracted serine protease inhibitors and anticoagulant proteins
US5872098A (en) * 1995-06-05 1999-02-16 Corvas International, Inc. Nematode-extracted anticoagulant protein
US6068995A (en) * 1994-10-25 2000-05-30 Yoshitomi Pharmaceutical Industries, Ltd. Method for producing protein
JPH10513057A (ja) * 1995-01-30 1998-12-15 ニューヨーク・ブラッド・センター・インコーポレイテッド 組換えα−ガラクトシダーゼ酵素
EP0852625A2 (de) * 1995-09-25 1998-07-15 Chiron Corporation Sekretorische 'leader'-sequenz aus pichia zur protein-expression
GB9526733D0 (en) 1995-12-30 1996-02-28 Delta Biotechnology Ltd Fusion proteins
MX9605082A (es) 1996-10-24 1998-04-30 Univ Autonoma De Nuevo Leon Levaduras metilotroficas modificadas geneticamente para la produccion y secrecion de hormona de crecimiento humano.
US6150081A (en) * 1997-12-24 2000-11-21 Fuji Photo Film B.V. Silver halide emulsions with recombinant collagen suitable for photographic application and also the preparation thereof
CN1105727C (zh) * 1998-06-17 2003-04-16 上海海济医药生物工程有限公司 重组人血清白蛋白的生产方法
GB9902000D0 (en) 1999-01-30 1999-03-17 Delta Biotechnology Ltd Process
CN1103374C (zh) * 1999-03-04 2003-03-19 上海贸基生物工程科技有限公司 重组dna技术生产人血清白蛋白的方法
CN1103373C (zh) * 1999-03-04 2003-03-19 上海贸基生物工程科技有限公司 人血清白蛋白改构基因、表达载体及其宿主
JP2003530838A (ja) 2000-04-12 2003-10-21 ヒューマン ゲノム サイエンシズ インコーポレイテッド アルブミン融合タンパク質
US6946134B1 (en) 2000-04-12 2005-09-20 Human Genome Sciences, Inc. Albumin fusion proteins
WO2002031178A1 (en) * 2000-10-12 2002-04-18 Uab Research Foundation An artificial gene and vectors for expressing high-yield recombinant ovine interferon-tau in pichia pastoris
US7507413B2 (en) 2001-04-12 2009-03-24 Human Genome Sciences, Inc. Albumin fusion proteins
ES2425738T3 (es) 2001-12-21 2013-10-17 Human Genome Sciences, Inc. Proteínas de fusión de la albúmina
EP1594530A4 (de) 2003-01-22 2006-10-11 Human Genome Sciences Inc Albuminfusionsproteine
US20070231856A1 (en) * 2003-05-30 2007-10-04 Pei Ye Recombinant Protein and Polypeptide Production Using Methylotropic or Ethylotropic Microorganisms with a Dilute Methanol or Ethanol Feeding
CN101979650B (zh) * 2003-10-22 2015-09-16 凯克研究生院 使用单倍体交配策略在酵母中合成异聚多亚基多肽的方法
RU2306333C2 (ru) * 2005-05-27 2007-09-20 Общество с ограниченной ответственностью научно-производственная компания "Генные и клеточные технологии" ШТАММ ДРОЖЖЕЙ Pichia pastoris Х-33/2albumin ДЛЯ СЕКРЕЦИИ ЧЕЛОВЕЧЕСКОГО АЛЬБУМИНА
KR20110020819A (ko) * 2008-05-15 2011-03-03 가부시키가이샤 아루떼꾸 우에노 안구건조증 및/또는 각막/결막 질환 치료용 약학 조성물
CN104704126B (zh) 2012-07-12 2018-03-20 Uab巴尔特玛斯公司 在酵母表达系统中利用人内质网分子伴侣蛋白天然信号序列产生天然重组的分泌的人内质网分子伴侣蛋白
PL2964765T3 (pl) * 2013-03-08 2019-10-31 Keck Graduate Inst Of Applied Life Sciences Promotory drożdżowe z pichia pastoris
CN103397036B (zh) * 2013-03-27 2014-12-10 广州白云山拜迪生物医药有限公司 一种用于毕赤酵母表达重组人血清白蛋白的基因序列
CN119039424A (zh) * 2024-08-13 2024-11-29 河北华凯惠合生物医药有限公司 一种高效获得高纯度重组人血清白蛋白的纯化工艺

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4414329A (en) * 1980-01-15 1983-11-08 Phillips Petroleum Company Biochemical conversions by yeast fermentation at high cell densities
IL66614A (en) * 1981-08-28 1985-09-29 Genentech Inc Method of constructing a dna sequence encoding a polypeptide,microbial production of human serum albumin,and pharmaceutical compositions comprising it
US4775622A (en) * 1982-03-08 1988-10-04 Genentech, Inc. Expression, processing and secretion of heterologous protein by yeast
US4808537A (en) * 1984-10-30 1989-02-28 Phillips Petroleum Company Methanol inducible genes obtained from pichia and methods of use
US4837148A (en) * 1984-10-30 1989-06-06 Phillips Petroleum Company Autonomous replication sequences for yeast strains of the genus pichia
US4855231A (en) * 1984-10-30 1989-08-08 Phillips Petroleum Company Regulatory region for heterologous gene expression in yeast
US4885242A (en) * 1984-10-30 1989-12-05 Phillips Petroleum Company Genes from pichia histidine pathway and uses thereof
JPS6229985A (ja) * 1985-06-17 1987-02-07 ジエネツクス・コ−ポレ−シヨン クロ−ン化プレプロ−ヒト血清アルブミン遺伝子およびヒト血清アルブミン遺伝子、それらの遺伝子を含むプラスミド、プラスミドにより形質転換された微生物、およびその微生物を用いたプレプロ−ヒト血清アルブミン遺伝子の製造方法
US4882279A (en) * 1985-10-25 1989-11-21 Phillips Petroleum Company Site selective genomic modification of yeast of the genus pichia
US4895800A (en) * 1985-11-26 1990-01-23 Phillips Petroleum Company Yeast production of hepatitis B surface antigen
GB8613388D0 (en) * 1986-06-03 1986-07-09 Delta Biotechnology Ltd Induction of galactose regulated gene expression in yeast
GB8615701D0 (en) * 1986-06-27 1986-08-06 Delta Biotechnology Ltd Stable gene integration vector
IL82935A0 (en) * 1986-08-12 1987-12-20 Phillips Petroleum Co Secretion of heterologous proteins from yeast
IL89992A0 (en) * 1988-04-25 1989-12-15 Phillips Petroleum Co Expression of human serum albumin in methylotrophic yeasts
CA2058820C (en) * 1991-04-25 2003-07-15 Kotikanyad Sreekrishna Expression cassettes and vectors for the secretion of human serum albumin in pichia pastoris cells

Also Published As

Publication number Publication date
EP0510678A2 (de) 1992-10-28
JP2004242678A (ja) 2004-09-02
DK0510678T3 (da) 1998-12-21
DE69224768D1 (de) 1998-04-23
EP0510678B1 (de) 1998-03-18
JP3579430B2 (ja) 2004-10-20
DE69224768T2 (de) 1998-07-09
JPH05115294A (ja) 1993-05-14
CA2059167A1 (en) 1992-10-27
CA2059167C (en) 2001-08-21
EP0510678A3 (en) 1993-12-29
US5330901A (en) 1994-07-19
ES2113387T3 (es) 1998-05-01

Similar Documents

Publication Publication Date Title
ATE164184T1 (de) Expression von menschlichen serum-albuminen in pichia pastoris
ATE159297T1 (de) Expression von menschlichen serumalbumin in methylotrophischen hefen
DE3762630D1 (de) Verfahren zur herstellung von diperoxidodecandionsaeure enthaltenden agglomeraten und die verwendung derselben in bleichmitteln.
DE69115765D1 (de) Herstellung von optisch aktiven arylaliphatischen Carbonsäuren
DE69224124D1 (de) Verfahren zur Herstellung von Dihomo-Gamma-Linolensäure und das in dieser enthaltende Lipid
ATE78399T1 (de) Verwendung von buspiron zur verbesserung des kurzzeitgedaechtnisses.
DE69126646D1 (de) Biologisches Verfahren zur Herstellung von optisch-aktiver Milchsäure
ATE140962T1 (de) Dns-klone von menschlichem thrombomodulin
DE68916754D1 (de) Verfahren zur Herstellung von optisch aktiver 3-Hydroxybuttersäure.
DE3771920D1 (de) P-hydroxyphenylsulfoniumsalze und deren verwendung bei der herstellung von estern und amiden.
DK0401896T3 (da) Carboplatinmateriale
FR2607517B2 (fr) Vecteurs d'expression de variants de l'hirudine dans les levures, procede et produit obtenu
DE69220113D1 (de) Verfahren zur Herstellung von menschlichem Serum Albumin
IT8722593A0 (it) Procedimento per l'iniettofusione di materie artificiali termoplastiche.
DE3851364D1 (de) Verfahren zur Herstellung von supraleitendem Material.
FI913566A7 (fi) Lisätty proteiinintuotanto eläimissä
ATE103989T1 (de) Monoklonale antikoerper gegen e7-protein des humanen papillomvirus typ 16, verfahren zu ihrer herstellung sowie ihre verwendung.
DE58907247D1 (de) Verfahren zur herstellung von dipeptiden mit c-terminalen nicht-proteinogenen aminosäuren.
DE68911836D1 (de) Benztriazolderivate und daraus abgeleitete Reagenzien zur Herstellung von chiralen Derivaten von Carbonsäuren.
DE58901435D1 (de) Verfahren zur herstellung von dipeptiden mit n-terminalen nichtproteinogenen aminosaeuren.
DE58905010D1 (de) Verfahren zur herstellung von 5-chlor-3-chlorsulfonyl-2-thiophencarbonsaeureestern.
NO171270C (no) Heteroatom inneholdende perfluoralkylterminerte neopentylsyrer og salter derav.
DE68904916D1 (de) Verfahren zur herstellung von wenig gefaerbten fettsaeureamiden.
DE69030774D1 (de) Optisch aktive Diastereoisomersalze von Tetrahydro-2-furancarbonsäure
DE68908843D1 (de) Verfahren zur Herstellung von 4,6-Dimethyl-7-hydroxynonan-3-on.

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification