ATE168194T1 - Molekulargewichtstrennung von biomolekülen durch kapillare elektrophorese unter verwendung einer polymerhaltigen lösung - Google Patents

Molekulargewichtstrennung von biomolekülen durch kapillare elektrophorese unter verwendung einer polymerhaltigen lösung

Info

Publication number
ATE168194T1
ATE168194T1 AT93910939T AT93910939T ATE168194T1 AT E168194 T1 ATE168194 T1 AT E168194T1 AT 93910939 T AT93910939 T AT 93910939T AT 93910939 T AT93910939 T AT 93910939T AT E168194 T1 ATE168194 T1 AT E168194T1
Authority
AT
Austria
Prior art keywords
solution containing
molecular weight
biomolecules
capillary electrophoresis
containing polymers
Prior art date
Application number
AT93910939T
Other languages
English (en)
Inventor
David M Demorest
William E Werner
John E Wiktorowicz
H-Michael Wenz
Frank L Oaks
Original Assignee
Perkin Elmer Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Perkin Elmer Corp filed Critical Perkin Elmer Corp
Application granted granted Critical
Publication of ATE168194T1 publication Critical patent/ATE168194T1/de

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416Systems
    • G01N27/447Systems using electrophoresis
    • G01N27/44704Details; Accessories
    • G01N27/44752Controlling the zeta potential, e.g. by wall coatings
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416Systems
    • G01N27/447Systems using electrophoresis
    • G01N27/44704Details; Accessories
    • G01N27/44747Composition of gel or of carrier mixture

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • General Physics & Mathematics (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Dispersion Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
  • Compositions Of Macromolecular Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Macromolecular Compounds Obtained By Forming Nitrogen-Containing Linkages In General (AREA)
AT93910939T 1992-05-01 1993-04-30 Molekulargewichtstrennung von biomolekülen durch kapillare elektrophorese unter verwendung einer polymerhaltigen lösung ATE168194T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/877,956 US5264101A (en) 1989-11-06 1992-05-01 Capillary electrophoresis molecular weight separation of biomolecules using a polymer-containing solution

Publications (1)

Publication Number Publication Date
ATE168194T1 true ATE168194T1 (de) 1998-07-15

Family

ID=25371084

Family Applications (1)

Application Number Title Priority Date Filing Date
AT93910939T ATE168194T1 (de) 1992-05-01 1993-04-30 Molekulargewichtstrennung von biomolekülen durch kapillare elektrophorese unter verwendung einer polymerhaltigen lösung

Country Status (6)

Country Link
US (1) US5264101A (de)
EP (1) EP0638168B1 (de)
JP (1) JP3176373B2 (de)
AT (1) ATE168194T1 (de)
DE (1) DE69319596T2 (de)
WO (1) WO1993022665A1 (de)

Families Citing this family (66)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5332481A (en) 1991-01-29 1994-07-26 Beckman Instruments, Inc. Capillary electrophoresis using replaceable gels
US5384024A (en) * 1992-03-13 1995-01-24 Applied Biosystems, Inc. Capillary electrophoresis
DE69415339T2 (de) * 1993-10-14 1999-06-02 Bio-Rad Laboratories, Inc., Hercules, Calif. Unterdrückung der elektroendosmose während der elektroforese in gellosen medien durch verwendung von geladenen polymeren
US5891313A (en) * 1993-11-23 1999-04-06 The Perkin-Elmer Corp. Entrapment of nucleic acid sequencing template in sample mixtures by entangled polymer networks
WO1995016910A1 (en) * 1993-12-17 1995-06-22 Perkin-Elmer Corporation Uncharged polymers for separation of biomolecules by capillary electrophoresis
US6358385B1 (en) * 1993-12-17 2002-03-19 The Perkin-Elmer Corporation Polymers for separation of biomolecules by capillary electrophoresis
US5423966A (en) * 1994-01-25 1995-06-13 Perkin-Elmer Corporation On line ion contaminant removal apparatus and method for capillary electrophoresis
US5503722A (en) * 1994-02-28 1996-04-02 Beckman Instruments, Inc. Rehydratable gels for capillary electrophoresis
WO1996023220A1 (en) 1995-01-27 1996-08-01 Northeastern University Polyvinyl alcohol (pva) based covalently bonded stable hydrophilic coating for capillary electrophoresis
US5582705A (en) * 1995-05-19 1996-12-10 Iowa State University Research Foundation, Inc. Multiplexed capillary electrophoresis system
JP2000502734A (ja) * 1995-12-28 2000-03-07 ノバルティス アクチエンゲゼルシャフト 分離剤として充填可能ポリマー溶液を使用するキャピラリー電気泳動分離法
US5942443A (en) 1996-06-28 1999-08-24 Caliper Technologies Corporation High throughput screening assay systems in microscale fluidic devices
US5989399A (en) * 1996-09-04 1999-11-23 The Research Foundation Of State University Of New York Effective surface treatment for a new separation medium in electrophoresis
US6001232A (en) * 1996-09-04 1999-12-14 The Research Foundation Of State University Of New York Separation medium for capillary electrophoresis
US5964995A (en) 1997-04-04 1999-10-12 Caliper Technologies Corp. Methods and systems for enhanced fluid transport
US5976336A (en) * 1997-04-25 1999-11-02 Caliper Technologies Corp. Microfluidic devices incorporating improved channel geometries
WO1998049548A1 (en) 1997-04-25 1998-11-05 Caliper Technologies Corporation Microfluidic devices incorporating improved channel geometries
US7033474B1 (en) 1997-04-25 2006-04-25 Caliper Life Sciences, Inc. Microfluidic devices incorporating improved channel geometries
US5958694A (en) 1997-10-16 1999-09-28 Caliper Technologies Corp. Apparatus and methods for sequencing nucleic acids in microfluidic systems
US5948227A (en) * 1997-12-17 1999-09-07 Caliper Technologies Corp. Methods and systems for performing electrophoretic molecular separations
US6013165A (en) * 1998-05-22 2000-01-11 Lynx Therapeutics, Inc. Electrophoresis apparatus and method
EP1084391A4 (de) 1998-06-08 2006-06-14 Caliper Life Sciences Inc Mikrofluidische geräte, systeme und verfahren zur durchführung integrierter reaktionen und trennungen
US6706161B1 (en) * 1998-06-10 2004-03-16 Agilent Technologies, Inc. Methods and compositions for modifying the surface of a polymeric substrate
US6800287B2 (en) * 1998-09-25 2004-10-05 Yeda Research And Development Co., Ltd. Copolymer 1 related polypeptides for use as molecular weight markers and for therapeutic use
CN1296703C (zh) 1999-02-02 2007-01-24 卡钳生命科学股份有限公司 鉴定蛋白质的方法、装置和系统
US6407000B1 (en) * 1999-04-09 2002-06-18 Micron Technology, Inc. Method and apparatuses for making and using bi-modal abrasive slurries for mechanical and chemical-mechanical planarization of microelectronic-device substrate assemblies
US6599836B1 (en) * 1999-04-09 2003-07-29 Micron Technology, Inc. Planarizing solutions, planarizing machines and methods for mechanical or chemical-mechanical planarization of microelectronic-device substrate assemblies
US6306273B1 (en) * 1999-04-13 2001-10-23 Aclara Biosciences, Inc. Methods and compositions for conducting processes in microfluidic devices
US6716948B1 (en) * 1999-07-31 2004-04-06 Symyx Technologies, Inc. Controlled-architecture polymers and use thereof as separation media
US6680208B1 (en) * 1999-11-19 2004-01-20 Becton, Dickinson And Company Rapid protein identification using antibody mixtures
US20020012971A1 (en) * 2000-03-20 2002-01-31 Mehta Tammy Burd PCR compatible nucleic acid sieving medium
CA2407124C (en) * 2000-04-25 2010-09-28 Spectrumedix Corporation Denaturant-free electrophoresis of biological molecules under high temperature conditions
FR2810905B1 (fr) * 2000-06-30 2003-05-16 Inst Curie Additif pour minimiser les phenomenes d'adsorption et/ou d'electroosmose
US6706162B1 (en) * 2000-09-25 2004-03-16 Applera Corporation High speed, high resolution compositions, methods, and kits for capillary electrophoresis
US20030085127A1 (en) * 2001-06-13 2003-05-08 Symyx Technologies, Inc. Polymer additive useful in separation media
JP4657524B2 (ja) * 2001-08-29 2011-03-23 嘉信 馬場 マイクロチップ電気泳動用装置及びそれを用いる電気泳動方法
US20050130157A1 (en) * 2002-01-22 2005-06-16 Philip Serwer Electrophoretic ratchets and cyclic electrophoresis
US20040029143A1 (en) * 2002-02-28 2004-02-12 Jeffrey Van Ness Cationic polyelectrolytes in biomolecule purification and analysis
DE10243930A1 (de) * 2002-03-08 2003-09-25 Conducta Endress & Hauser Vorrichtung zur Messung und Regelung des pH-Wertes eines Mediums
US7381317B2 (en) * 2002-08-12 2008-06-03 Beckman Coulter, Inc. Methods and compositions for capillary electrophoresis (CE)
US7083711B2 (en) * 2003-02-21 2006-08-01 Combisep, Inc. Capillary electrophoresis gel especially for separation made for single stranded nucleic acid separations
US20050025741A1 (en) 2003-05-15 2005-02-03 Lau Aldrich N.K. Poly and copoly(N-vinylamide)s and their use in capillary electrophoresis
US20070272556A1 (en) * 2003-05-22 2007-11-29 Kane Thomas E Electrophoresis Capillaries Having Reduced Amounts of -Oh
JP4450368B2 (ja) 2004-03-04 2010-04-14 独立行政法人産業技術総合研究所 マイクロ流路チップの製造方法、マイクロ流路チップ、そのマイクロ流路チップを用いる生体分子の分離方法、およびそのマイクロ流路チップを有する電気泳動装置
US7846676B2 (en) 2004-07-19 2010-12-07 Cell Biosciences, Inc. Methods and devices for analyte detection
US7935479B2 (en) * 2004-07-19 2011-05-03 Cell Biosciences, Inc. Methods and devices for analyte detection
KR100647306B1 (ko) * 2004-12-23 2006-11-23 삼성전자주식회사 아미노기와 카르복실기를 포함하고 제1 pH에서 양전하를띠는 물질을 이용하여 핵산을 분리하는 방법
JP4645255B2 (ja) * 2005-03-24 2011-03-09 株式会社島津製作所 分析試料調製用溶媒
CN101237934B (zh) * 2005-05-21 2012-12-19 先进液体逻辑公司 用亲水性聚合物助剂减弱生物分子的吸附
US20070014699A1 (en) 2005-06-23 2007-01-18 Beckman Coulter, Inc, Methods and apparatus for improving the sensitivity of capillary zone electrophoresis
JP4167296B2 (ja) * 2005-08-01 2008-10-15 株式会社ナード研究所 電気泳動用ポリアクリルアミドゲル、それを用いたポリアクリルアミドゲル電気泳動方法、その製造方法、およびアクリルアミド化合物
WO2007035864A2 (en) 2005-09-20 2007-03-29 Cell Biosciences, Inc. Electrophoresis standards, methods and kits
US10107782B2 (en) 2008-01-25 2018-10-23 ProteinSimple Method to perform limited two dimensional separation of proteins and other biologicals
US9841401B2 (en) 2009-04-14 2017-12-12 National Cheng Kung University Capillary electrophoresis method for analyzing collagen
US20100258439A1 (en) * 2009-04-14 2010-10-14 Huang Lynn Ling-Huei Capillary electrophoresis method for analyzing collagen and an assay kit for the same
CA2825149C (en) 2011-01-31 2020-06-16 Biotype Gmbh Reduced artifact denaturing capillary electrophoresis of nucleic acids
WO2013155634A1 (en) 2012-04-19 2013-10-24 Jiaqi Wu Dual wavelength isoelectric focusing for determining drug load in antibody drug conjugates
JP6348258B2 (ja) * 2013-06-20 2018-06-27 大塚電子株式会社 分離分析方法
US9766206B2 (en) 2013-09-27 2017-09-19 ProteinSimple Apparatus, systems, and methods for capillary electrophoresis
WO2018051374A1 (en) * 2016-09-15 2018-03-22 Dianax S.R.L. Microfluidic device, particularly of the lab-on-chip type, for the concentration, purification, and detection of biological and/or medical targets of interest from a biological sample
US11946900B2 (en) * 2018-04-09 2024-04-02 Shimadzu Corporation Electrophoresis flow channel cleaning method and electrophoresis device
CA3120895C (en) * 2018-12-20 2024-07-02 Sergey N. Krylov BINDER SELECTION USING CAPILLARY ELECTROPHORESIS
EP3757176B1 (de) * 2019-06-27 2022-08-31 Canon Production Printing Holding B.V. Tintenstrahlbeschichtungszusammensetzung
US10985617B1 (en) 2019-12-31 2021-04-20 Energous Corporation System for wirelessly transmitting energy at a near-field distance without using beam-forming control
CA3252671A1 (en) * 2022-02-28 2023-08-31 Revvity Health Sciences, Inc. METHODS FOR SEPARATION AND DETECTION OF DOUBLE-STRED AND SINGLE-STRED RIBONUCLEIC ACID (RNA)
US20260049958A1 (en) * 2022-10-25 2026-02-19 Synthon B.V. Capillary gel electrophoresis separation of diastereomers of phosphorothioated oligonucleotides

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4405798A (en) * 1981-03-30 1983-09-20 Celanese Corporation Acrylate and methacrylate monoesters of pentaerythritol and pentaerythritol orthoesters and polymers and copolymers derived therefrom
JPS6060548A (ja) * 1983-09-14 1985-04-08 Fuji Photo Film Co Ltd 電気泳動用媒体
JPS6060549A (ja) * 1983-09-14 1985-04-08 Fuji Photo Film Co Ltd ゲル電気泳動用媒体
US4690749A (en) * 1985-12-16 1987-09-01 Universities Space Research Association Polymer-coated surfaces to control surface zeta potential
US4997537A (en) * 1986-10-21 1991-03-05 Northeastern University High performance microcapillary gel electrophoresis
US4931328A (en) * 1988-08-19 1990-06-05 Hewlett-Packard Company Capillary tube with reduced protein interactions and controllable electroosmotic flow
EP0417925A3 (en) * 1989-09-12 1991-09-11 Northeastern University High performance microcapillary gel electrophoresis
DE69007773T2 (de) * 1990-02-13 1994-09-22 Bio Rad Laboratories Elektrophoretische Trennung in einem gelfreien Medium mittels gelösten Polymeren.
US5069766A (en) * 1990-12-20 1991-12-03 Bio-Rad Laboratories, Inc. Suppression of electroendosmosis in capillary electrophoresis

Also Published As

Publication number Publication date
WO1993022665A1 (en) 1993-11-11
DE69319596T2 (de) 1999-03-04
EP0638168A1 (de) 1995-02-15
US5264101A (en) 1993-11-23
EP0638168A4 (de) 1995-07-19
EP0638168B1 (de) 1998-07-08
JP3176373B2 (ja) 2001-06-18
DE69319596D1 (de) 1998-08-13
JPH07506432A (ja) 1995-07-13

Similar Documents

Publication Publication Date Title
ATE168194T1 (de) Molekulargewichtstrennung von biomolekülen durch kapillare elektrophorese unter verwendung einer polymerhaltigen lösung
SE9602638D0 (sv) An improved method for the capillary electrophoresis of nucleic acids, proteins and low molecular charged compounds
DK260485A (da) Kationisk polyelektrolyt med hoej molekylvaegt samt fremgangsmaade til fremstilling deraf
DE69033795D1 (de) Oberflächenladungsbeschichtung für kapillare elektrophorese
NO158877C (no) Fremgangsmaate for fremstilling av monomere eller polymereforbindelser med isocyanursyregrupper.
DE3674197D1 (de) Wasserabsorbierende acrylcopolymere.
PT83351B (pt) Processo de preparacao de uma composicao baseada numa dispersao aquosa de um polimero de adicao de monomeros olefinicamente insaturados, de material de revestimento e de um adesivo
KR880013890A (ko) 가교 결합 이미도페닐아민(Imidophenylamines)
ES2053711T3 (es) Electrolito polimerico solido y generadores electroquimicos que lo contengan.
NO179000C (no) Betongtaksten og fremgangsmåte for fremstilling derav
KR890003847A (ko) 중합성 기판 제품
DE3681569D1 (de) Dispersionspolymere, verfahren zu ihrer herstellung und ihre verwendung.
EP0193154A3 (en) Potential-causing element for immunosensor
ATE39259T1 (de) Wasserabsorbierende harze, verfahren zu deren herstellung und deren verwendung bei der herstellung von waessrigen fluidaabsorbierenden gegenstaenden.
SE8105599L (sv) Framstellning av hydrofila polymergeler med hog molekylvikt
ES2001474A6 (es) Procedimiento para preparar una composicion de copolimero vinilico de injerto. para revestir substratos, incluyendo metales y vidrio.
KR840001446A (ko) 금속제 병의 제조방법
KR870006139A (ko) 도금용 폴리페닐렌 설파이드 수지 조성물
FI871957L (fi) Foerfarande foer framstaellning av polymerer och kopolymerer av amidosulfonsyra innehaollande monomerer och deras salter.
FR2509912B1 (fr) Pile electro-chimique au calcium
FI912482A0 (fi) Metod foer separering av organiska substanser.
DE3681210D1 (de) Verfahren zur herstellung wasserabsorbierender harze.
ATE89847T1 (de) Epoxidharzmassen mit verbesserter lagerstabilitaet.
BR8502583A (pt) Aderente para a ligacao de superficies em municoes contendo cargas explosivas
Finkler et al. Permanent coated capillaries with reversed electroosmotic flow for anion analysis

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties