ATE171221T1 - Elektrochemische behandlung von nukleinsäure - enthaltenden lösungen - Google Patents
Elektrochemische behandlung von nukleinsäure - enthaltenden lösungenInfo
- Publication number
- ATE171221T1 ATE171221T1 AT93902446T AT93902446T ATE171221T1 AT E171221 T1 ATE171221 T1 AT E171221T1 AT 93902446 T AT93902446 T AT 93902446T AT 93902446 T AT93902446 T AT 93902446T AT E171221 T1 ATE171221 T1 AT E171221T1
- Authority
- AT
- Austria
- Prior art keywords
- nucleic acid
- pct
- sec
- solutions containing
- containing nucleic
- Prior art date
Links
- 150000007523 nucleic acids Chemical class 0.000 title abstract 5
- 102000039446 nucleic acids Human genes 0.000 title abstract 5
- 108020004707 nucleic acids Proteins 0.000 title abstract 5
- 238000000034 method Methods 0.000 abstract 4
- 239000000463 material Substances 0.000 abstract 2
- JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical compound [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 abstract 1
- 230000003321 amplification Effects 0.000 abstract 1
- 150000001768 cations Chemical class 0.000 abstract 1
- 238000004925 denaturation Methods 0.000 abstract 1
- 230000036425 denaturation Effects 0.000 abstract 1
- 238000001514 detection method Methods 0.000 abstract 1
- 238000007834 ligase chain reaction Methods 0.000 abstract 1
- 229910001425 magnesium ion Inorganic materials 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
- 238000003752 polymerase chain reaction Methods 0.000 abstract 1
- 239000000523 sample Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6862—Ligase chain reaction [LCR]
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
- Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
- Y10T436/143333—Saccharide [e.g., DNA, etc.]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB929201481A GB9201481D0 (en) | 1992-01-23 | 1992-01-23 | Treatment of nucleic acid material |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE171221T1 true ATE171221T1 (de) | 1998-10-15 |
Family
ID=10709139
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT93902446T ATE171221T1 (de) | 1992-01-23 | 1993-01-22 | Elektrochemische behandlung von nukleinsäure - enthaltenden lösungen |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US5607832A (de) |
| EP (1) | EP0636185B1 (de) |
| JP (1) | JP3086255B2 (de) |
| AT (1) | ATE171221T1 (de) |
| AU (1) | AU3362893A (de) |
| CA (1) | CA2128686C (de) |
| DE (1) | DE69321112D1 (de) |
| GB (1) | GB9201481D0 (de) |
| SG (1) | SG49027A1 (de) |
| WO (1) | WO1993015224A1 (de) |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5824477A (en) * | 1990-09-12 | 1998-10-20 | Scientific Generics Limited | Electrochemical denaturation of double-stranded nucleic acid |
| US6197508B1 (en) | 1990-09-12 | 2001-03-06 | Affymetrix, Inc. | Electrochemical denaturation and annealing of nucleic acid |
| EP0702728B1 (de) * | 1993-06-09 | 1998-04-15 | Gamera Bioscience Corporation | Magnetische zyklusreaktionen |
| WO1995025177A1 (en) | 1994-03-15 | 1995-09-21 | Scientific Generics Limited | Electrochemical denaturation of double-stranded nucleic acid |
| US5686271A (en) * | 1994-06-09 | 1997-11-11 | Gamera Bioscience Corporation | Apparatus for performing magnetic cycle reaction |
| JP3751979B2 (ja) | 1995-08-25 | 2006-03-08 | アフィメトリックス，インコーポレイテッド | 細胞内物質の放出 |
| US6300063B1 (en) | 1995-11-29 | 2001-10-09 | Affymetrix, Inc. | Polymorphism detection |
| US6953663B1 (en) * | 1995-11-29 | 2005-10-11 | Affymetrix, Inc. | Polymorphism detection |
| GB9609815D0 (en) * | 1996-05-10 | 1996-07-17 | Scient Generics Ltd | Denaturation of double-stranded nucleic acids |
| GB9613980D0 (en) * | 1996-07-02 | 1996-09-04 | Scient Generics Ltd | Denaturation of double-stranded nucleic acid |
| GB9614544D0 (en) * | 1996-07-11 | 1996-09-04 | Scient Generics Ltd | Denaturation of double-stranded nucleic acid |
| GB9706654D0 (en) | 1997-04-02 | 1997-05-21 | Scient Generics Ltd | Disassociation of interacting molecules |
| AU735293B2 (en) * | 1997-04-04 | 2001-07-05 | Innogenetics N.V. | Isothermal polymerase chain reaction by cycling the concentration of divalent metal ions |
| US5965410A (en) * | 1997-09-02 | 1999-10-12 | Caliper Technologies Corp. | Electrical current for controlling fluid parameters in microchannels |
| US5993611A (en) * | 1997-09-24 | 1999-11-30 | Sarnoff Corporation | Capacitive denaturation of nucleic acid |
| US6174675B1 (en) | 1997-11-25 | 2001-01-16 | Caliper Technologies Corp. | Electrical current for controlling fluid parameters in microchannels |
| US20040185462A1 (en) * | 1999-08-06 | 2004-09-23 | Tum Gene, Inc. | Method of and detecting apparatus and detecting chip for single base substitution SNP and point mutation of genes |
| ATE408141T1 (de) | 1999-10-20 | 2008-09-15 | Shigeori Takenaka | Gen-detektionschip, sensor und nachweisverfahren |
| AU2003256675A1 (en) * | 2002-07-26 | 2004-02-16 | Applera Corporation | Mg-mediated hot start biochemical reactions |
| US20050003396A1 (en) * | 2003-04-11 | 2005-01-06 | Mihrimah Ozkan | Biosensors having single reactant components immobilized over single electrodes and methods of making and using thereof |
| WO2006051988A1 (ja) * | 2004-11-15 | 2006-05-18 | Riken | 核酸の増幅方法 |
| JP5165383B2 (ja) * | 2004-12-23 | 2013-03-21 | アイ−スタツト・コーポレイシヨン | 分子診断システム及び方法 |
| US8703445B2 (en) | 2005-12-29 | 2014-04-22 | Abbott Point Of Care Inc. | Molecular diagnostics amplification system and methods |
| US20090233809A1 (en) * | 2008-03-04 | 2009-09-17 | Affymetrix, Inc. | Resequencing methods for identification of sequence variants |
| US20100151473A1 (en) * | 2008-12-10 | 2010-06-17 | Yeakley Joanne M | Methods and compositions for hybridizing nucleic acids |
| CN113671167B (zh) * | 2020-05-14 | 2024-08-02 | 北京元芯碳基集成电路研究院 | 生物电子器件及其制备方法和可控转化方法 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB8417301D0 (en) * | 1984-07-06 | 1984-08-08 | Serono Diagnostics Ltd | Assay |
| US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4787963A (en) * | 1987-05-04 | 1988-11-29 | Syntro Corporation | Method and means for annealing complementary nucleic acid molecules at an accelerated rate |
| AU622426B2 (en) * | 1987-12-11 | 1992-04-09 | Abbott Laboratories | Assay using template-dependent nucleic acid probe reorganization |
| AU8517391A (en) * | 1990-09-12 | 1992-03-30 | Scientific Generics Limited | Electrochemical denaturation of double-stranded nucleic acid |
| US5527670A (en) * | 1990-09-12 | 1996-06-18 | Scientific Generics Limited | Electrochemical denaturation of double-stranded nucleic acid |
-
1992
- 1992-01-23 GB GB929201481A patent/GB9201481D0/en active Pending
-
1993
- 1993-01-22 US US08/256,784 patent/US5607832A/en not_active Expired - Lifetime
- 1993-01-22 AT AT93902446T patent/ATE171221T1/de not_active IP Right Cessation
- 1993-01-22 CA CA002128686A patent/CA2128686C/en not_active Expired - Lifetime
- 1993-01-22 DE DE69321112T patent/DE69321112D1/de not_active Expired - Lifetime
- 1993-01-22 JP JP05513027A patent/JP3086255B2/ja not_active Expired - Fee Related
- 1993-01-22 AU AU33628/93A patent/AU3362893A/en not_active Abandoned
- 1993-01-22 EP EP93902446A patent/EP0636185B1/de not_active Expired - Lifetime
- 1993-01-22 WO PCT/GB1993/000147 patent/WO1993015224A1/en not_active Ceased
- 1993-01-22 SG SG1996005294A patent/SG49027A1/en unknown
-
1996
- 1996-12-05 US US08/759,646 patent/US5814450A/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| DE69321112D1 (de) | 1998-10-22 |
| US5607832A (en) | 1997-03-04 |
| CA2128686A1 (en) | 1993-08-05 |
| JP3086255B2 (ja) | 2000-09-11 |
| CA2128686C (en) | 2005-07-05 |
| EP0636185A1 (de) | 1995-02-01 |
| US5814450A (en) | 1998-09-29 |
| SG49027A1 (en) | 1998-05-18 |
| GB9201481D0 (en) | 1992-03-11 |
| JPH07503370A (ja) | 1995-04-13 |
| WO1993015224A1 (en) | 1993-08-05 |
| AU3362893A (en) | 1993-09-01 |
| EP0636185B1 (de) | 1998-09-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ATE171221T1 (de) | Elektrochemische behandlung von nukleinsäure - enthaltenden lösungen | |
| DE69507289D1 (de) | Elektrochemische denatusierung von doppelsträngiger nukleinsäure | |
| Eichhorn | Metal ions as stabilizers or destabilizers of the deoxyribonucleic acid structure | |
| DE69016079D1 (de) | Methode zur Extraktion, Amplifikation und zum Nachweis einer Nukleinsäure aus einer Fraktion mononuklearer Zellen von peripherem Blut. | |
| DE69009774D1 (de) | Verfahren zum klonieren und reagenziensatz. | |
| ATE266723T1 (de) | Lösung die chaotropische mittel enthält und verwendung dieser lösung in verfahren zur isolierung von dna, rna und proteinen | |
| DE69627698T2 (de) | Eine in kaskaden verlaufende vervielfältigungsreaktion von nukleinsäuren | |
| ATE243759T1 (de) | Verfahren zur dämpfung der fluoreszene in lösung von fluoreszenzmarkierten oligonucleotidsonden | |
| DE69133204D1 (en) | Gereinigtes thermostabiles nukleinsäure-polymeraseenzym aus thermotoga maritima | |
| ATE206764T1 (de) | Verfahren zum sensitiven nachweis von nukleinsäuren | |
| AU6180190A (en) | Fast process for detecting and/or identifying a single base on a nucleic acid sequence and its applications | |
| ES2194843T3 (es) | Deteccion de acidos nucleicos en sangre. | |
| DE3750774D1 (de) | Chaotropisches verfahren zur schätzung von nukleinen säuren in einer biologischen probe. | |
| ATE191513T1 (de) | Verfahren zur amplifikation von nukleinsäuren | |
| ATE252110T1 (de) | Markierte oligonukleotide | |
| DE69837913D1 (de) | Verfahren zur vervielfältigung von nukleinsäure | |
| KR910008135A (ko) | 단백질 분해 효소를 사용하지 않는 핵산의 추출 및 pcr 증폭방법 | |
| ATE353973T1 (de) | Die verwendung der 3'-wesentlichen bearbeitungswirksamkeit von dns-polymerase | |
| ES2080807T3 (es) | Procedimiento para controlar la contaminacion de las reacciones de multiplicacion de acidos nucleicos dependientes de oligonucleotidos. | |
| NO179953C (no) | Kvantitativ metode for bestemmelse av 1,4-dihydronikotinamid-adenin-dinukleotid i opplösning, samt engangs enzymelektrode | |
| ES2144466T3 (es) | Metodo de tratamiento de muestras de esputo que contienen mycobacterias. | |
| KR920701477A (ko) | 핵산의 증폭기술에 대한 개량 | |
| DE3588154D1 (de) | Schnellere Methode für die Reassoziation von Nukleinsäure | |
| ATE325893T1 (de) | Fluorometrisches verfahren zur überwachung und zum nachweis von nucleinsäure-amplifizierung | |
| US20010008762A1 (en) | Electrode capture of nucleic acid |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| REN | Ceased due to non-payment of the annual fee |