ATE175239T1 - Sialidase defiziente zellen - Google Patents

Sialidase defiziente zellen

Info

Publication number
ATE175239T1
ATE175239T1 AT94916789T AT94916789T ATE175239T1 AT E175239 T1 ATE175239 T1 AT E175239T1 AT 94916789 T AT94916789 T AT 94916789T AT 94916789 T AT94916789 T AT 94916789T AT E175239 T1 ATE175239 T1 AT E175239T1
Authority
AT
Austria
Prior art keywords
sialidase
dna
sialidase deficiency
cells
disrupted
Prior art date
Application number
AT94916789T
Other languages
English (en)
Inventor
Thomas G Warner
Mary B Sliwkowski
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Application granted granted Critical
Publication of ATE175239T1 publication Critical patent/ATE175239T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01018—Exo-alpha-sialidase (3.2.1.18), i.e. trans-sialidase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Fats And Perfumes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT94916789T 1993-05-17 1994-05-17 Sialidase defiziente zellen ATE175239T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US6258693A 1993-05-17 1993-05-17
US18732794A 1994-01-25 1994-01-25

Publications (1)

Publication Number Publication Date
ATE175239T1 true ATE175239T1 (de) 1999-01-15

Family

ID=26742448

Family Applications (2)

Application Number Title Priority Date Filing Date
AT98106858T ATE474925T1 (de) 1993-05-17 1994-05-17 Rekombinante cho zell sialidase
AT94916789T ATE175239T1 (de) 1993-05-17 1994-05-17 Sialidase defiziente zellen

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT98106858T ATE474925T1 (de) 1993-05-17 1994-05-17 Rekombinante cho zell sialidase

Country Status (13)

Country Link
US (1) US5928915A (de)
EP (2) EP0866130B1 (de)
JP (1) JPH08510133A (de)
AT (2) ATE474925T1 (de)
AU (2) AU6834994A (de)
CA (1) CA2160458A1 (de)
DE (2) DE69415669T2 (de)
DK (2) DK0866130T3 (de)
ES (2) ES2128562T3 (de)
GR (1) GR3029781T3 (de)
NZ (1) NZ266714A (de)
PT (1) PT866130E (de)
WO (1) WO1994026908A1 (de)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6562588B2 (en) * 1993-05-17 2003-05-13 Genentech, Inc. Sialidase and recombinant cell lines
JP3088681B2 (ja) * 1997-05-22 2000-09-18 宮城県 形質膜局在シアリダーゼ及びそれをコードするｄｎａ
US6261805B1 (en) * 1999-07-15 2001-07-17 Boyce Thompson Institute For Plant Research, Inc. Sialyiation of N-linked glycoproteins in the baculovirus expression vector system
KR100604258B1 (ko) * 1999-12-30 2006-07-31 씨제이 주식회사 당단백질의 생체내 활성을 증가시키는 방법
AU2002222436A1 (en) * 2001-12-14 2003-06-30 Provost, Fellows And Scholars Of The College Of The Holy And Undivided Trinity Of Queen Elizabeth Near Dublin A reporter gene
EP1711512B1 (de) * 2004-01-30 2012-01-11 Amgen Inc. Verfahren zur reinigung von proteinen
WO2007108464A1 (ja) * 2006-03-20 2007-09-27 Kyowa Hakko Kogyo Co., Ltd. 哺乳動物由来細胞質シアリダーゼに対する抗体
CA2676686C (en) * 2007-02-20 2015-12-15 Dsm Ip Assets B.V. Novel sialidase
JP4772905B2 (ja) 2007-04-02 2011-09-14 協和発酵キリン株式会社 アンチトロンビン組成物の製造方法
EP2930245B1 (de) * 2007-10-12 2017-11-22 Sigma-Aldrich Co. LLC Zelllinie und verfahren zur verbesserten sialylierung von glycoproteinen
JP2008263996A (ja) * 2008-06-02 2008-11-06 National Institute Of Advanced Industrial & Technology 発光甲虫由来赤色発光酵素安定体の生産及び精製法
US11060083B2 (en) 2013-07-19 2021-07-13 Larix Bioscience Llc Methods and compositions for producing double allele knock outs
JP6482546B2 (ja) * 2013-07-19 2019-03-13 ラリクス・バイオサイエンス・リミテッド・ライアビリティ・カンパニーＬａｒｉｘ Ｂｉｏｓｃｉｅｎｃｅ， Ｌｌｃ 二重対立遺伝子ノックアウトを生成するための方法および組成物
CN120904322A (zh) 2013-09-13 2025-11-07 豪夫迈·罗氏有限公司 包含纯化的重组多肽的方法和组合物
RU2016107435A (ru) 2013-09-13 2017-10-18 Дженентек, Инк. Композиции и способы обнаружения и количественного определения белка клеток-хозяев в клеточных линиях и рекомбинантные полипептидные продукты
CN114258431A (zh) * 2019-07-03 2022-03-29 帕利昂制药有限公司 重组人唾液酸酶、唾液酸酶融合蛋白及其使用方法
CN112795583A (zh) * 2020-11-16 2021-05-14 上海大学 重组唾液酸外切酶的制备方法、表达基因、重组表达载体及构建方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4912040A (en) * 1986-11-14 1990-03-27 Genetics Institute, Inc. Eucaryotic expression system
US5075218A (en) * 1987-12-29 1991-12-24 Biomira, Inc. Screening for antibodies which bind carbohydrate epitopes of tumor-associated antigens, and uses thereof
US5047335A (en) * 1988-12-21 1991-09-10 The Regents Of The University Of Calif. Process for controlling intracellular glycosylation of proteins
WO1993010260A1 (en) * 1991-11-21 1993-05-27 The Board Of Trustees Of The Leland Stanford Junior University Controlling degradation of glycoprotein oligosaccharides by extracellular glycosisases

Also Published As

Publication number Publication date
GR3029781T3 (en) 1999-06-30
EP0700443B1 (de) 1998-12-30
ES2346941T3 (es) 2010-10-21
DE69435304D1 (de) 2010-09-02
EP0866130B1 (de) 2010-07-21
EP0700443A1 (de) 1996-03-13
PT866130E (pt) 2010-10-26
DE69415669T2 (de) 1999-07-01
AU7841998A (en) 1998-10-01
EP0866130A1 (de) 1998-09-23
US5928915A (en) 1999-07-27
NZ266714A (en) 1997-12-19
WO1994026908A1 (en) 1994-11-24
DK0866130T3 (da) 2010-10-04
DK0700443T3 (da) 1999-08-30
JPH08510133A (ja) 1996-10-29
DE69415669D1 (de) 1999-02-11
CA2160458A1 (en) 1994-11-24
AU6834994A (en) 1994-12-12
ES2128562T3 (es) 1999-05-16
ATE474925T1 (de) 2010-08-15

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