ATE189264T1 - Chimärisches oligonukleotid und dessen verwendung zur herstellung von nukleinsäure-transkripten - Google Patents
Chimärisches oligonukleotid und dessen verwendung zur herstellung von nukleinsäure-transkriptenInfo
- Publication number
- ATE189264T1 ATE189264T1 AT95402150T AT95402150T ATE189264T1 AT E189264 T1 ATE189264 T1 AT E189264T1 AT 95402150 T AT95402150 T AT 95402150T AT 95402150 T AT95402150 T AT 95402150T AT E189264 T1 ATE189264 T1 AT E189264T1
- Authority
- AT
- Austria
- Prior art keywords
- oligonucleotide
- target
- sequence
- segment
- nucleic acid
- Prior art date
Links
- 108091034117 Oligonucleotide Proteins 0.000 title abstract 8
- 108020004707 nucleic acids Proteins 0.000 title abstract 2
- 150000007523 nucleic acids Chemical class 0.000 title abstract 2
- 102000039446 nucleic acids Human genes 0.000 title abstract 2
- 230000000694 effects Effects 0.000 abstract 4
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 abstract 2
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 abstract 2
- 230000003321 amplification Effects 0.000 abstract 2
- 230000000295 complement effect Effects 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 2
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 abstract 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 102100034343 Integrase Human genes 0.000 abstract 1
- 101710203526 Integrase Proteins 0.000 abstract 1
- 125000004122 cyclic group Chemical group 0.000 abstract 1
- 238000013518 transcription Methods 0.000 abstract 1
- 230000035897 transcription Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR9411455A FR2724934B1 (fr) | 1994-09-26 | 1994-09-26 | Oligonucleotide chimere et son utilisation dans l'obtention de transcrits d'un acide nucleique |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE189264T1 true ATE189264T1 (de) | 2000-02-15 |
Family
ID=9467268
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT95402150T ATE189264T1 (de) | 1994-09-26 | 1995-09-26 | Chimärisches oligonukleotid und dessen verwendung zur herstellung von nukleinsäure-transkripten |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US5744308A (de) |
| EP (1) | EP0721988B1 (de) |
| AT (1) | ATE189264T1 (de) |
| CA (1) | CA2159064A1 (de) |
| DE (1) | DE69514773T2 (de) |
| DK (1) | DK0721988T3 (de) |
| ES (1) | ES2144110T3 (de) |
| FR (1) | FR2724934B1 (de) |
Families Citing this family (47)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6001610A (en) * | 1994-11-23 | 1999-12-14 | Roche Diagnostics, Gmbh | Method for the particularly sensitive detection of nucleic acids |
| FR2737223B1 (fr) * | 1995-07-24 | 1997-09-12 | Bio Merieux | Procede d'amplification de sequences d'acide nucleique par deplacement, a l'aide d'amorces chimeres |
| US5853990A (en) * | 1996-07-26 | 1998-12-29 | Edward E. Winger | Real time homogeneous nucleotide assay |
| US6537783B1 (en) | 1996-08-02 | 2003-03-25 | Bio Merieux | Prefunctionalized nucleotide and process for amplifying a sequence using a prefunctionalized nucleotide |
| AU748847B2 (en) * | 1998-02-27 | 2002-06-13 | Pamgene B.V. | Method for the non-specific amplification of nucleic acid |
| FR2780059B1 (fr) * | 1998-06-17 | 2002-10-11 | Bio Merieux | Procede de marquage d'un acide ribonucleique et fragments d'arn marques ainsi obtenus |
| US6232104B1 (en) | 1999-08-17 | 2001-05-15 | Dade Behring Inc. | Detection of differences in nucleic acids by inhibition of spontaneous DNA branch migration |
| US6677445B1 (en) | 1999-08-27 | 2004-01-13 | Chiron Corporation | Chimeric antisense oligonucleotides and cell transfecting formulations thereof |
| US6692918B2 (en) * | 1999-09-13 | 2004-02-17 | Nugen Technologies, Inc. | Methods and compositions for linear isothermal amplification of polynucleotide sequences |
| US6251639B1 (en) | 1999-09-13 | 2001-06-26 | Nugen Technologies, Inc. | Methods and compositions for linear isothermal amplification of polynucleotide sequences, using a RNA-DNA composite primer |
| US7846733B2 (en) * | 2000-06-26 | 2010-12-07 | Nugen Technologies, Inc. | Methods and compositions for transcription-based nucleic acid amplification |
| JP2004513617A (ja) * | 2000-06-26 | 2004-05-13 | ニューゲン テクノロジーズ, インコーポレイテッド | 転写に基づく核酸増幅のための方法および組成物 |
| ES2300366T3 (es) * | 2000-09-02 | 2008-06-16 | Grunenthal Gmbh | Oligonucleotidos antisentido contra el vr1. |
| CA2436693A1 (en) | 2000-12-11 | 2002-06-20 | Alexion Pharmaceuticals, Inc. | Nested oligonucleotides containing hairpin for nucleic acid amplification |
| AU2002228974A1 (en) * | 2000-12-13 | 2002-06-24 | Nugen Technologies, Inc | Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof |
| US6946251B2 (en) | 2001-03-09 | 2005-09-20 | Nugen Technologies, Inc. | Methods and compositions for amplification of RNA sequences using RNA-DNA composite primers |
| CA2439074A1 (en) * | 2001-03-09 | 2002-09-19 | Nugen Technologies, Inc. | Methods and compositions for amplification of rna sequences |
| US7056671B2 (en) * | 2001-08-20 | 2006-06-06 | Takara Bio Inc. | Isothermal chimeric primer nucleic acid amplification methods using blocking oglionucleotide |
| US7414111B2 (en) * | 2001-09-19 | 2008-08-19 | Alexion Pharmaceuticals, Inc. | Engineered templates and their use in single primer amplification |
| CA2460639C (en) * | 2001-09-19 | 2013-07-16 | Alexion Pharmaceuticals, Inc. | Engineered templates and their use in single primer amplification |
| US20040054162A1 (en) * | 2001-10-30 | 2004-03-18 | Hanna Michelle M. | Molecular detection systems utilizing reiterative oligonucleotide synthesis |
| US7045319B2 (en) * | 2001-10-30 | 2006-05-16 | Ribomed Biotechnologies, Inc. | Molecular detection systems utilizing reiterative oligonucleotide synthesis |
| US7176025B2 (en) * | 2002-03-11 | 2007-02-13 | Nugen Technologies, Inc. | Methods for generating double stranded DNA comprising a 3′ single stranded portion and uses of these complexes for recombination |
| GB0230238D0 (en) * | 2002-12-28 | 2003-02-05 | Fu Guoliang | Combined exponential and linear amplification |
| US20070128598A1 (en) * | 2002-10-30 | 2007-06-07 | Piet Boender | Methods for generating rna copies |
| EP1576188B1 (de) * | 2002-11-21 | 2008-10-15 | Epicentre Technologies | Verfahren zur verwendung von riboprimern zur strangverdrängungsreplikation von zielsequenzen |
| EP1597388A4 (de) * | 2002-12-20 | 2006-06-07 | Q Rna Inc | Verfahren und kits zur herstellung doppelsträngiger ribonukleinsäuren |
| WO2004092418A2 (en) * | 2003-04-14 | 2004-10-28 | Nugen Technologies, Inc. | Global amplification using a randomly primed composite primer |
| CA2544367A1 (en) * | 2003-10-29 | 2005-05-12 | Ribomed Biotechnologies, Inc. | Compositions, methods and detection technologies for reiterative oligonucleotide synthesis |
| EP1929046B1 (de) * | 2005-09-07 | 2012-07-11 | Nugen Technologies, Inc. | Verbessertes nukleinsäureamplifikationsverfahren |
| EP1921156A1 (de) * | 2006-11-10 | 2008-05-14 | bioMerieux B.V. | Verbesserte multiplexe Nukleinsäureamplifikation mittels blockierter Primer |
| US20090203531A1 (en) * | 2008-02-12 | 2009-08-13 | Nurith Kurn | Method for Archiving and Clonal Expansion |
| WO2009117698A2 (en) | 2008-03-21 | 2009-09-24 | Nugen Technologies, Inc. | Methods of rna amplification in the presence of dna |
| US8911948B2 (en) * | 2008-04-30 | 2014-12-16 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
| US9434988B2 (en) | 2008-04-30 | 2016-09-06 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
| US10227641B2 (en) | 2008-04-30 | 2019-03-12 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
| DK2644707T3 (en) | 2008-04-30 | 2015-06-29 | Integrated Dna Tech Inc | RNase-H-based assays using modified RNA monomers. |
| WO2014143228A1 (en) * | 2013-03-15 | 2014-09-18 | Integrated Dna Technologies, Inc. | Rnase h-based assays utilizing modified rna monomers |
| US8242243B2 (en) | 2008-05-15 | 2012-08-14 | Ribomed Biotechnologies, Inc. | Methods and reagents for detecting CpG methylation with a methyl CpG binding protein (MBP) |
| US8211644B2 (en) * | 2008-07-13 | 2012-07-03 | Ribomed Biotechnologies, Inc. | Molecular beacon-based methods for detection of targets using abscription |
| WO2010107716A2 (en) | 2009-03-15 | 2010-09-23 | Ribomed Biotechnologies, Inc. | Abscription based molecular detection |
| US20110117559A1 (en) * | 2009-11-13 | 2011-05-19 | Integrated Dna Technologies, Inc. | Small rna detection assays |
| US20130217071A1 (en) | 2011-12-30 | 2013-08-22 | Luz Montesclaros | Methods and compositions for performing nucleic acid amplification reactions |
| WO2014085687A1 (en) | 2012-11-28 | 2014-06-05 | Abwiz Bio, Inc. | Preparation of gene-specific templates for the use in single primer amplification |
| WO2020106906A1 (en) * | 2018-11-21 | 2020-05-28 | Avida Biomed, Inc. | Methods for targeted nucleic acid library formation |
| EP3978626A1 (de) * | 2020-10-02 | 2022-04-06 | Lexogen GmbH | Verfahren und mittel zur erzeugung von transkribierten nukleinsäuren |
| CN116323976A (zh) * | 2020-10-02 | 2023-06-23 | 莱克斯奥根有限公司 | 产生转录核酸的方法和工具 |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL86724A (en) * | 1987-06-19 | 1995-01-24 | Siska Diagnostics Inc | Methods and kits for amplification and testing of nucleic acid sequences |
| IE72468B1 (en) * | 1987-07-31 | 1997-04-09 | Univ Leland Stanford Junior | Selective amplification of target polynucleotide sequences |
| EP0359789B1 (de) * | 1988-01-21 | 1993-08-04 | Genentech, Inc. | Verstärkung und nachweis von nukleinsäuresequenzen |
| CA1340807C (en) * | 1988-02-24 | 1999-11-02 | Lawrence T. Malek | Nucleic acid amplification process |
| US5130238A (en) * | 1988-06-24 | 1992-07-14 | Cangene Corporation | Enhanced nucleic acid amplification process |
| CA2001110A1 (en) * | 1988-11-18 | 1990-05-18 | Zvi G. Loewy | Compositions and process for amplifying and detecting nucleic acid sequences |
| KR0148265B1 (ko) * | 1988-12-16 | 1998-10-15 | 에프.지이.엠 헤르만스 | 자가-지속 서열 복제 시스템 |
| US5194370A (en) * | 1990-05-16 | 1993-03-16 | Life Technologies, Inc. | Promoter ligation activated transcription amplification of nucleic acid sequences |
| AU8997991A (en) * | 1991-01-31 | 1992-08-06 | Becton Dickinson & Company | Exonuclease mediated strand displacement amplification |
| US5169766A (en) * | 1991-06-14 | 1992-12-08 | Life Technologies, Inc. | Amplification of nucleic acid molecules |
| KR100316498B1 (ko) * | 1992-08-04 | 2002-06-20 | 다니엘 엘. 캐시앙, 헨리 엘. 노르호프, 피터 알. 쉬어리 | 핵산서열증폭방법 |
-
1994
- 1994-09-26 FR FR9411455A patent/FR2724934B1/fr not_active Expired - Fee Related
-
1995
- 1995-09-25 CA CA002159064A patent/CA2159064A1/fr not_active Abandoned
- 1995-09-26 AT AT95402150T patent/ATE189264T1/de not_active IP Right Cessation
- 1995-09-26 EP EP95402150A patent/EP0721988B1/de not_active Expired - Lifetime
- 1995-09-26 DE DE69514773T patent/DE69514773T2/de not_active Expired - Lifetime
- 1995-09-26 ES ES95402150T patent/ES2144110T3/es not_active Expired - Lifetime
- 1995-09-26 US US08/533,912 patent/US5744308A/en not_active Expired - Lifetime
- 1995-09-26 DK DK95402150T patent/DK0721988T3/da active
Also Published As
| Publication number | Publication date |
|---|---|
| DE69514773T2 (de) | 2000-09-07 |
| DK0721988T3 (da) | 2000-07-24 |
| CA2159064A1 (fr) | 1996-03-27 |
| EP0721988A1 (de) | 1996-07-17 |
| DE69514773D1 (de) | 2000-03-02 |
| FR2724934B1 (fr) | 1997-01-24 |
| EP0721988B1 (de) | 2000-01-26 |
| ES2144110T3 (es) | 2000-06-01 |
| FR2724934A1 (fr) | 1996-03-29 |
| US5744308A (en) | 1998-04-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |