ATE220426T1 - Verfahren zur direkten, exponentiellen amplifikation und sequenzierung von dna-molekülen und seine anwendung - Google Patents

Verfahren zur direkten, exponentiellen amplifikation und sequenzierung von dna-molekülen und seine anwendung

Info

Publication number
ATE220426T1
ATE220426T1 AT97122380T AT97122380T ATE220426T1 AT E220426 T1 ATE220426 T1 AT E220426T1 AT 97122380 T AT97122380 T AT 97122380T AT 97122380 T AT97122380 T AT 97122380T AT E220426 T1 ATE220426 T1 AT E220426T1
Authority
AT
Austria
Prior art keywords
sequencing
dna molecules
direct
exponential amplification
application
Prior art date
Application number
AT97122380T
Other languages
English (en)
Inventor
Svante Erik Prof Dr Paeaebo
Christian Alexis Dr Kilger
Original Assignee
Roche Diagnostics Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics Gmbh filed Critical Roche Diagnostics Gmbh
Application granted granted Critical
Publication of ATE220426T1 publication Critical patent/ATE220426T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/143—Concentration of primer or probe

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
AT97122380T 1996-12-20 1997-12-18 Verfahren zur direkten, exponentiellen amplifikation und sequenzierung von dna-molekülen und seine anwendung ATE220426T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19653439A DE19653439A1 (de) 1996-12-20 1996-12-20 Verfahren zur direkten, exponentiellen Amplifikation und Sequenzierung von DNA Molekülen und dessen Anwendung

Publications (1)

Publication Number Publication Date
ATE220426T1 true ATE220426T1 (de) 2002-07-15

Family

ID=7815636

Family Applications (2)

Application Number Title Priority Date Filing Date
AT97122380T ATE220426T1 (de) 1996-12-20 1997-12-18 Verfahren zur direkten, exponentiellen amplifikation und sequenzierung von dna-molekülen und seine anwendung
AT99123807T ATE227347T1 (de) 1996-12-20 1997-12-18 Verfahren zur direkten, exponentiellen amplifikation und sequenzierung von dna-molekülen und seine anwendung

Family Applications After (1)

Application Number Title Priority Date Filing Date
AT99123807T ATE227347T1 (de) 1996-12-20 1997-12-18 Verfahren zur direkten, exponentiellen amplifikation und sequenzierung von dna-molekülen und seine anwendung

Country Status (6)

Country Link
US (3) US6107032A (de)
EP (2) EP1004677B1 (de)
JP (1) JPH10295400A (de)
AT (2) ATE220426T1 (de)
DE (3) DE19653439A1 (de)
ES (1) ES2186293T3 (de)

Families Citing this family (89)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6214555B1 (en) * 1996-05-01 2001-04-10 Visible Genetics Inc. Method compositions and kit for detection
US6605428B2 (en) * 1996-12-20 2003-08-12 Roche Diagnostics Gmbh Method for the direct, exponential amplification and sequencing of DNA molecules and its application
US20030215857A1 (en) * 1996-12-20 2003-11-20 Roche Diagnostics Gmbh Method for the direct, exponential amplification and sequencing of DNA molecules and its application
DE19653439A1 (de) * 1996-12-20 1998-07-02 Svante Dr Paeaebo Verfahren zur direkten, exponentiellen Amplifikation und Sequenzierung von DNA Molekülen und dessen Anwendung
US6828094B2 (en) * 1996-12-20 2004-12-07 Roche Diagnostics Gmbh Method for the uncoupled, direct, exponential amplification and sequencing of DNA molecules with the addition of a second thermostable DNA polymerase and its application
JP3435416B2 (ja) * 1997-03-12 2003-08-11 ピーイー コーポレイション（エヌワイ） 改善された標識ヌクレオチド取込み特性を有するｄｎａポリメラーゼ
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
DE19848403A1 (de) * 1998-10-21 2000-04-27 Guenter Von Kiedrowski Verfahren zur exponentiellen Amplifikation molekularer Matrizen
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
ATE262595T1 (de) 1999-09-13 2004-04-15 Nugen Technologies Inc Methoden und zusammensetzungen zur linearen isothermalen amplifikation von polynukleotidsequenzen
US6692918B2 (en) 1999-09-13 2004-02-17 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
US7846733B2 (en) 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
EP1356094B1 (de) * 2000-06-26 2010-01-13 Nugen Technologies, Inc. Methoden und zusammensetzungen zur auf transkription basierenden vervielfältigung von nukleinsäuren
US7494817B2 (en) * 2000-09-06 2009-02-24 Transnet Yx, Inc. Methods for genotype screening using magnetic particles
EP1978110B1 (de) * 2000-09-06 2010-05-26 Transnetyx, Inc. Computer-basiertes Verfahren und System zum Screenen von genomischer DNS
US20050266494A1 (en) * 2000-09-06 2005-12-01 Hodge Timothy A System and method for computer network ordering of biological testing
US20030087286A1 (en) * 2000-09-06 2003-05-08 Hodge Timothy A. Isolation of eukaryotic genomic DNA using magnetically responsive solid functionalized particles
US20030082605A1 (en) * 2000-09-06 2003-05-01 Hodge Timothy A. Genomic DNA detection method and system thereof
US20050272085A1 (en) * 2000-09-06 2005-12-08 Hodge Timothy A Methods for forensic and congenic screening
US20050239125A1 (en) * 2000-09-06 2005-10-27 Hodge Timothy A Methods for genotype screening
EP1427847B1 (de) 2000-12-13 2010-07-28 Nugen Technologies, Inc. Methoden und zusammensetzungen zur generierung einer vielzahl von kopien von nukleinsäuresequenzen und methoden zur detektion derselben
US7060434B2 (en) * 2001-02-14 2006-06-13 Council Of Scientific & Industrial Research Probes for myctophid fish and a method for developing the same
MXPA02012739A (es) 2001-03-09 2004-04-20 Nugen Technologies Inc Metodos y composiciones para amplificacion de secuencias de arn.
EP1390537B1 (de) * 2001-03-09 2013-11-13 Nugen Technologies, Inc. Verfahren und zusammensetzungen zur amplifikation von rna-sequenzen
JP2004523243A (ja) 2001-03-12 2004-08-05 カリフォルニア インスティチュート オブ テクノロジー 非同期性塩基伸長によってポリヌクレオチド配列を分析するための方法および装置
US20060014186A1 (en) * 2001-09-04 2006-01-19 Hodge Timothy A Methods for genotype screening of a strain disposed on an adsorbent carrier
US20050042629A1 (en) 2002-09-13 2005-02-24 Applera Corporation Thermus scotoductus nucleic acid polymerases
EP1472335A4 (de) * 2001-11-08 2005-12-28 Univ Johns Hopkins Verfahren und systeme zur nukleinsäuresequenzierung
AU2002346498A1 (en) 2001-11-30 2003-06-17 Applera Corporation Thermus brockianus nucleic acid polymerases
US20040096826A1 (en) * 2002-01-30 2004-05-20 Evans Glen A. Methods for creating recombination products between nucleotide sequences
US7776524B2 (en) 2002-02-15 2010-08-17 Genzyme Corporation Methods for analysis of molecular events
JP2006523465A (ja) * 2003-04-14 2006-10-19 ニューゲン テクノロジーズ， インコーポレイテッド ランダムにプライミングされる複合プライマーを用いる大規模増幅
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
ATE463584T1 (de) 2004-02-19 2010-04-15 Helicos Biosciences Corp Verfahren zur analyse von polynukleotidsequenzen
US7476734B2 (en) 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
EP1766090B1 (de) 2004-05-25 2011-04-27 Helicos Biosciences Corporation Verfahren zur nukleinsäureimmobilisierung
US8536661B1 (en) 2004-06-25 2013-09-17 University Of Hawaii Biosensor chip sensor protection methods
JP4476050B2 (ja) * 2004-06-30 2010-06-09 株式会社ニデック 視野計
WO2006026654A2 (en) 2004-08-27 2006-03-09 Exact Sciences Corporation Method for detecting a recombinant event
WO2006047787A2 (en) 2004-10-27 2006-05-04 Exact Sciences Corporation Method for monitoring disease progression or recurrence
US7785785B2 (en) 2004-11-12 2010-08-31 The Board Of Trustees Of The Leland Stanford Junior University Charge perturbation detection system for DNA and other molecules
US7220549B2 (en) 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US7482120B2 (en) 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
US9777314B2 (en) 2005-04-21 2017-10-03 Esoterix Genetic Laboratories, Llc Analysis of heterogeneous nucleic acid samples
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
EP1929046B1 (de) 2005-09-07 2012-07-11 Nugen Technologies, Inc. Verbessertes nukleinsäureamplifikationsverfahren
US7397546B2 (en) 2006-03-08 2008-07-08 Helicos Biosciences Corporation Systems and methods for reducing detected intensity non-uniformity in a laser beam
US11339430B2 (en) 2007-07-10 2022-05-24 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
EP2677309B9 (de) 2006-12-14 2014-11-19 Life Technologies Corporation Verfahren zur Sequenzierung einer Nukleinsäure mittels grosser FET-Arrays, geeignet zur Messung in einem begrenzten pH-Bereich
US7932034B2 (en) 2006-12-20 2011-04-26 The Board Of Trustees Of The Leland Stanford Junior University Heat and pH measurement for sequencing of DNA
NZ584656A (en) * 2007-11-09 2012-08-31 Mitomics Inc Mitochondrial dna deletion between about residues 12317-16254 for use in the detection of cancer
US8034568B2 (en) 2008-02-12 2011-10-11 Nugen Technologies, Inc. Isothermal nucleic acid amplification methods and compositions
US7846666B2 (en) 2008-03-21 2010-12-07 Nugen Technologies, Inc. Methods of RNA amplification in the presence of DNA
WO2010008480A2 (en) 2008-06-25 2010-01-21 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes using large scale fet arrays
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US20100301398A1 (en) 2009-05-29 2010-12-02 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US8673627B2 (en) 2009-05-29 2014-03-18 Life Technologies Corporation Apparatus and methods for performing electrochemical reactions
US20120261274A1 (en) 2009-05-29 2012-10-18 Life Technologies Corporation Methods and apparatus for measuring analytes
US8776573B2 (en) 2009-05-29 2014-07-15 Life Technologies Corporation Methods and apparatus for measuring analytes
US20120001646A1 (en) 2010-06-30 2012-01-05 Life Technologies Corporation Methods and apparatus for testing isfet arrays
JP2013533482A (ja) 2010-06-30 2013-08-22 ライフ テクノロジーズ コーポレーション イオン感応性電荷蓄積回路および方法
US8432149B2 (en) 2010-06-30 2013-04-30 Life Technologies Corporation Array column integrator
US11307166B2 (en) 2010-07-01 2022-04-19 Life Technologies Corporation Column ADC
CN103168341B (zh) 2010-07-03 2016-10-05 生命科技公司 具有轻度掺杂的排出装置的化学敏感的传感器
EP2617061B1 (de) 2010-09-15 2021-06-30 Life Technologies Corporation Verfahren und vorrichtung zur analytmessung
CN103299182A (zh) 2010-09-24 2013-09-11 生命科技公司 匹配的晶体管对电路
DE102011083555B4 (de) 2011-09-27 2013-10-10 Aspre Ag Analyseverfahren und Analysevorrichtung
US9970984B2 (en) 2011-12-01 2018-05-15 Life Technologies Corporation Method and apparatus for identifying defects in a chemical sensor array
US8821798B2 (en) 2012-01-19 2014-09-02 Life Technologies Corporation Titanium nitride as sensing layer for microwell structure
US8747748B2 (en) 2012-01-19 2014-06-10 Life Technologies Corporation Chemical sensor with conductive cup-shaped sensor surface
US8786331B2 (en) 2012-05-29 2014-07-22 Life Technologies Corporation System for reducing noise in a chemical sensor array
US9080968B2 (en) 2013-01-04 2015-07-14 Life Technologies Corporation Methods and systems for point of use removal of sacrificial material
US9841398B2 (en) 2013-01-08 2017-12-12 Life Technologies Corporation Methods for manufacturing well structures for low-noise chemical sensors
US8962366B2 (en) 2013-01-28 2015-02-24 Life Technologies Corporation Self-aligned well structures for low-noise chemical sensors
US8841217B1 (en) 2013-03-13 2014-09-23 Life Technologies Corporation Chemical sensor with protruded sensor surface
US8963216B2 (en) 2013-03-13 2015-02-24 Life Technologies Corporation Chemical sensor with sidewall spacer sensor surface
WO2014149780A1 (en) 2013-03-15 2014-09-25 Life Technologies Corporation Chemical sensor with consistent sensor surface areas
US9116117B2 (en) 2013-03-15 2015-08-25 Life Technologies Corporation Chemical sensor with sidewall sensor surface
EP2972281B1 (de) 2013-03-15 2023-07-26 Life Technologies Corporation Chemische vorrichtung mit dünnem leitenden element
CN105283758B (zh) 2013-03-15 2018-06-05 生命科技公司 具有一致传感器表面区域的化学传感器
US9835585B2 (en) 2013-03-15 2017-12-05 Life Technologies Corporation Chemical sensor with protruded sensor surface
US20140336063A1 (en) 2013-05-09 2014-11-13 Life Technologies Corporation Windowed Sequencing
US10458942B2 (en) 2013-06-10 2019-10-29 Life Technologies Corporation Chemical sensor array having multiple sensors per well
US10077472B2 (en) 2014-12-18 2018-09-18 Life Technologies Corporation High data rate integrated circuit with power management
TWI756167B (zh) 2014-12-18 2022-03-01 美商生命技術公司 積體電路裝置、感測器裝置及積體電路
CN107407656B (zh) 2014-12-18 2020-04-07 生命科技公司 使用大规模 fet 阵列测量分析物的方法和装置

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5693517A (en) * 1987-06-17 1997-12-02 Roche Molecular Systems, Inc. Reagents and methods for coupled high temperature reverse transcription and polymerase chain reactions
US4962020A (en) * 1988-07-12 1990-10-09 President And Fellows Of Harvard College DNA sequencing
US5075216A (en) * 1988-09-23 1991-12-24 Cetus Corporation Methods for dna sequencing with thermus aquaticus dna polymerase
US5427911A (en) * 1990-05-01 1995-06-27 Yale University Coupled amplification and sequencing of DNA
CA2114124A1 (en) * 1991-07-24 1993-02-04 Keith Gregg Single step amplification and sequencing of nucleic acids
FI923911L (fi) * 1992-09-01 1994-03-02 Vsevolod Kiselev DNA-molekylers in vitro-syntes
US5338671A (en) * 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
US5432065A (en) * 1993-03-30 1995-07-11 United States Biochemical Corporation Cycle sequencing with non-thermostable DNA polymerases
US5556772A (en) * 1993-12-08 1996-09-17 Stratagene Polymerase compositions and uses thereof
US5512462A (en) * 1994-02-25 1996-04-30 Hoffmann-La Roche Inc. Methods and reagents for the polymerase chain reaction amplification of long DNA sequences
US5614365A (en) * 1994-10-17 1997-03-25 President & Fellow Of Harvard College DNA polymerase having modified nucleotide binding site for DNA sequencing
US5789168A (en) * 1996-05-01 1998-08-04 Visible Genetics Inc. Method for amplification and sequencing of nucleic acid polymers
US5830657A (en) * 1996-05-01 1998-11-03 Visible Genetics Inc. Method for single-tube sequencing of nucleic acid polymers
US5928906A (en) * 1996-05-09 1999-07-27 Sequenom, Inc. Process for direct sequencing during template amplification
DE19653439A1 (de) * 1996-12-20 1998-07-02 Svante Dr Paeaebo Verfahren zur direkten, exponentiellen Amplifikation und Sequenzierung von DNA Molekülen und dessen Anwendung

Also Published As

Publication number Publication date
ES2186293T3 (es) 2003-05-01
EP0849364A1 (de) 1998-06-24
DE69713866D1 (de) 2002-08-14
EP1004677B1 (de) 2002-11-06
US20040197811A1 (en) 2004-10-07
US6107032A (en) 2000-08-22
JPH10295400A (ja) 1998-11-10
ATE227347T1 (de) 2002-11-15
EP1004677A1 (de) 2000-05-31
EP0849364B1 (de) 2002-07-10
US20020192661A1 (en) 2002-12-19
DE69716966T2 (de) 2003-08-28
DE69716966D1 (de) 2002-12-12
DE69713866T2 (de) 2003-02-06
DE19653439A1 (de) 1998-07-02

Similar Documents

Publication Publication Date Title
ATE227347T1 (de) Verfahren zur direkten, exponentiellen amplifikation und sequenzierung von dna-molekülen und seine anwendung
DE69702333D1 (de) Verfahren zur entkoppelten, direkten, exponentiellen Amplifikation und Sequenzierung von DNA-Molekülen unter Zugabe einer zweiten thermostabile DNA-Polymerase und ihre Anwendung
DE69725076D1 (de) Modifizierte thermostabile DNA Polymerase und eine DNA Polymerasezusammensetzung zur Amplifikation von Nukleinsäuren
DE69636080D1 (de) Eine in Kaskaden verlaufende Vervielfältigungsreaktion von Nukleinsäuren
DE69333891D1 (de) Verfahren zur Nukleinsäurenamplifizierung, Zusammensetzung und Kit
DE69708865D1 (de) Verfahren zur direkten sequenzierung während "template"-amplifikation
ATE191934T1 (de) Verfahren zur isothermer amplifikation von nukleinsäure molekülen
ATE159762T1 (de) Verfahren zur dns-sequenzbestimmung mit dns- polymerase von thermus aquaticus
ATE211179T1 (de) Amplifizierung von langen nukleinsäuresequenzen mit pcr
DE69523360D1 (de) Isotherme Amplifikation von Nukleinsäuren durch Strangverdrängung
FI960540A0 (fi) Eksonukleaasin ja/tai glykosylaasin käyttö antipolymeraasivasta-aineenlisäaineina spesifisyyden lisäämiseksi polymeraasiketjureaktiossa
DE69034177D1 (de) Verfahren zur Amplifikation von Nukleinsäuresequenzen
DE3750774D1 (de) Chaotropisches verfahren zur schätzung von nukleinen säuren in einer biologischen probe.
EP1072678A4 (de) Verfahren zum synthetisieren von dna
ATE244759T1 (de) Stabilisierte zusammensetzungen von reverser transkriptase und rna polymerase für nukleinsäureamplifikation
ATE266102T1 (de) Verfahren zur koamplifikation einer zielnukleinsäure unter versendung eines volumenabscheidungsmittel in der reaktionszusammensetzung, testsatz und testvorrichtung
ATE184321T1 (de) Dns-abhängige rns-polymerase-transkripte als reportermoleküle zur signalverstärkung in nukleinsäure-hybridisierungsuntersuchungen
AU6594694A (en) Reagents and methods for coupled high temperature reverse transcription and polymerase chain reactions
DE69834506D1 (de) Fluorometrisches verfahren zur überwachung und zum nachweis von nucleinsäure-amplifizierung
DE69938774D1 (de) Verfahren zur isolierung, amplifizierung und charakterisierung von dns
DE50008101D1 (de) Verfahren zur Aufreinigung von Nukleinsäuren sowie Anionenaustauscher zur Durchführung dieses Verfahrens
ATE197481T1 (de) Verfahren und reagenzien zur typisierung von hla- genen der klasse i
EP0704540A3 (de) Verfahren für die Einordnung von Gerste- und Malzsorten mittels Gendiagnose und ein dafür bestimmter Primer
DE69833864D1 (de) Methoden zur dns vervielfältigung und material dafür
DE60015210D1 (de) Oligonukleotid Primer zur effizienten reversen Transkription von Hepatitis C Virus (HCV) RNS und Verfahren zu deren Verwendung

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties