ATE231920T1 - Methode um mrna zu klonieren - Google Patents

Methode um mrna zu klonieren

Info

Publication number
ATE231920T1
ATE231920T1 AT93907440T AT93907440T ATE231920T1 AT E231920 T1 ATE231920 T1 AT E231920T1 AT 93907440 T AT93907440 T AT 93907440T AT 93907440 T AT93907440 T AT 93907440T AT E231920 T1 ATE231920 T1 AT E231920T1
Authority
AT
Austria
Prior art keywords
sequence
primer contains
primer
mrna
another approach
Prior art date
Application number
AT93907440T
Other languages
English (en)
Inventor
Peng Liang
Arthur B Pardee
Cesario F Bianchi
Original Assignee
Dana Farber Cancer Inst Inc
Harvard College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US07/850,343 external-priority patent/US5262311A/en
Application filed by Dana Farber Cancer Inst Inc, Harvard College filed Critical Dana Farber Cancer Inst Inc
Application granted granted Critical
Publication of ATE231920T1 publication Critical patent/ATE231920T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
AT93907440T 1992-03-11 1993-03-11 Methode um mrna zu klonieren ATE231920T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US07/850,343 US5262311A (en) 1992-03-11 1992-03-11 Methods to clone polyA mRNA
US3308493A 1993-03-11 1993-03-11
PCT/US1993/002246 WO1993018176A1 (en) 1992-03-11 1993-03-11 METHODS TO CLONE mRNA

Publications (1)

Publication Number Publication Date
ATE231920T1 true ATE231920T1 (de) 2003-02-15

Family

ID=26709258

Family Applications (1)

Application Number Title Priority Date Filing Date
AT93907440T ATE231920T1 (de) 1992-03-11 1993-03-11 Methode um mrna zu klonieren

Country Status (7)

Country Link
US (3) US5599672A (de)
EP (1) EP0592626B1 (de)
JP (1) JP2843675B2 (de)
AT (1) ATE231920T1 (de)
CA (1) CA2102784A1 (de)
DE (1) DE69332665T2 (de)
WO (1) WO1993018176A1 (de)

Families Citing this family (154)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5545522A (en) 1989-09-22 1996-08-13 Van Gelder; Russell N. Process for amplifying a target polynucleotide sequence using a single primer-promoter complex
US7049102B1 (en) 1989-09-22 2006-05-23 Board Of Trustees Of Leland Stanford University Multi-gene expression profile
US5795714A (en) 1992-11-06 1998-08-18 Trustees Of Boston University Method for replicating an array of nucleic acid probes
US5580726A (en) * 1994-04-29 1996-12-03 Geron Corporation Method and Kit for enhanced differential display
US5744300A (en) * 1993-03-24 1998-04-28 Geron Corporation Methods and reagents for the identification and regulation of senescence-related genes
DE4317414C1 (de) * 1993-05-18 1994-04-21 Max Planck Gesellschaft Mittel zur komplexen Diagnostik der Genexpression und Verfahren zur Anwendung für die medizinische Diagnostik und die Genisolierung
WO1995011923A1 (en) * 1993-10-29 1995-05-04 Dana-Farber Cancer Institute, Inc. A novel tumor marker and novel method of isolating same
US6110680A (en) * 1993-11-12 2000-08-29 The Scripps Research Institute Method for simultaneous identification of differentially expressed mRNAs and measurement of relative concentrations
US5459037A (en) * 1993-11-12 1995-10-17 The Scripps Research Institute Method for simultaneous identification of differentially expressed mRNAs and measurement of relative concentrations
US6096503A (en) * 1993-11-12 2000-08-01 The Scripps Research Institute Method for simultaneous identification of differentially expresses mRNAs and measurement of relative concentrations
US20110097791A1 (en) * 1999-04-16 2011-04-28 Engelhardt Dean L Novel process, construct and conjugate for producing multiple nucleic acid copies
US6986985B1 (en) * 1994-01-13 2006-01-17 Enzo Life Sciences, Inc. Process for producing multiple nucleic acid copies in vivo using a protein-nucleic acid construct
US20050123926A1 (en) * 1994-01-13 2005-06-09 Enzo Diagnostics, Inc., In vitro process for producing multiple nucleic acid copies
US6379897B1 (en) * 2000-11-09 2002-04-30 Nanogen, Inc. Methods for gene expression monitoring on electronic microarrays
RU2111254C1 (ru) * 1994-07-11 1998-05-20 Институт молекулярной биологии им.В.А.Энгельгардта РАН СПОСОБ ДЕТЕКЦИИ ДИФФЕРЕНЦИАЛЬНО ЭКСПРЕССИРУЮЩИХСЯ МАТРИЧНЫХ РНК И КЛОНИРОВАНИЯ СООТВЕТСТВУЮЩИХ ИМ ФРАГМЕНТОВ кДНК
DE69621507T2 (de) * 1995-03-28 2003-01-09 Japan Science And Technology Corp., Kawaguchi Verfahren zur molekularen Indexierung von Genen unter Verwendung von Restriktionsenzymen
DE19518505A1 (de) * 1995-05-19 1996-11-21 Max Planck Gesellschaft Verfahren zur Genexpressionsanalyse
US20030032610A1 (en) * 1996-06-03 2003-02-13 Gilchrest Barbara A. Method to inhibit cell growth using oligonucleotides
US6147056A (en) * 1995-06-06 2000-11-14 Trustees Of Boston University Use of locally applied DNA fragments
US7094766B1 (en) 1995-06-06 2006-08-22 Trustees Of Boston University Use of locally applied DNA fragments
US6218529B1 (en) * 1995-07-31 2001-04-17 Urocor, Inc. Biomarkers and targets for diagnosis, prognosis and management of prostate, breast and bladder cancer
AU2264197A (en) 1996-02-09 1997-08-28 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The Restriction display (rd-pcr) of differentially expressed mrnas
US6045998A (en) * 1996-04-03 2000-04-04 Johnson & Johnson Consumer Products, Inc. Technique for differential display
US5776683A (en) * 1996-07-11 1998-07-07 California Pacific Medical Center Methods for identifying genes amplified in cancer cells
EP0811696A3 (de) * 1996-06-06 1998-01-07 Smithkline Beecham Corporation Verfahren zur Gewinnung von Nukleotidsequenzen durch "randomly primed" Amplifikation
AU6497296A (en) * 1996-07-16 1998-02-09 Periannan Senapathy Method for contiguous genome sequencing
CA2744096C (en) * 1996-07-31 2013-07-30 Laboratory Corporation Of America Holdings Biomarkers and targets for diagnosis, prognosis and management of prostate disease
JPH1057064A (ja) * 1996-08-16 1998-03-03 Rikagaku Kenkyusho 微量遺伝子産物の特異的増幅方法
AU4174397A (en) * 1996-08-30 1998-03-19 Life Technologies, Inc. Methods for identification and isolation of specific nucleotide sequences in cdna and genomic dna
US20040142327A1 (en) * 1996-11-22 2004-07-22 Jhy-Jhu Lin Methods for identifying and isolating DNA polymorphisms or genetic markers
US6306588B1 (en) 1997-02-07 2001-10-23 Invitrogen Corporation Polymerases for analyzing or typing polymorphic nucleic acid fragments and uses thereof
WO1998039480A1 (en) * 1997-03-03 1998-09-11 Haqqi Tariq M Methods and compositions for identifying expressed genes
US6162616A (en) * 1997-04-16 2000-12-19 Millennium Pharmaceuticals, Inc. Multidrug resistance-associated polypeptide
AU7265298A (en) * 1997-04-29 1998-11-24 Trustees Of Boston University Methods and compositions for targeted dna differential display
CA2287876A1 (en) * 1997-04-30 1998-11-05 F. Hoffmann-La Roche Ag Rat st38.2 chemokine
US6261770B1 (en) 1997-05-13 2001-07-17 Display Systems Biotech Aps Method to clone mRNAs
ES2163271T3 (es) * 1997-05-13 2002-01-16 Azign Bioscience As Procedimiento para clonar arnm y visualizar transcriptores diferencialmente expresados (dodets).
US5994076A (en) * 1997-05-21 1999-11-30 Clontech Laboratories, Inc. Methods of assaying differential expression
US6489455B2 (en) 1997-05-21 2002-12-03 Clontech Laboratories, Inc. Methods of assaying differential expression
EP1007739A2 (de) * 1997-09-05 2000-06-14 Sidney Kimmel Cancer Center Auswahl von pcr primerpaaren zur amplifikation einer gruppe von nukleotidsequenzen
US7031843B1 (en) 1997-09-23 2006-04-18 Gene Logic Inc. Computer methods and systems for displaying information relating to gene expression data
US6150096A (en) * 1997-09-26 2000-11-21 Universite De Sherbrooke Molecular markers for the diagnosis of human diseases including Crohn's disease
US6897066B1 (en) 1997-09-26 2005-05-24 Athersys, Inc. Compositions and methods for non-targeted activation of endogenous genes
US6277571B1 (en) 1997-10-03 2001-08-21 Virginia Commonwealth University Intellectual Property Foundation Sequential consensus region-directed amplification of known and novel members of gene families
US6090553A (en) * 1997-10-29 2000-07-18 Beckman Coulter, Inc. Use of uracil-DNA glycosylase in genetic analysis
US6087102A (en) * 1998-01-07 2000-07-11 Clontech Laboratories, Inc. Polymeric arrays and methods for their use in binding assays
DE19806431C1 (de) * 1998-02-17 1999-10-14 Novartis Ag Neues Verfahren zur Identifikation und Charakterisierung von mRNA-Molekülen
US6365346B1 (en) 1998-02-18 2002-04-02 Dade Behring Inc. Quantitative determination of nucleic acid amplification products
US6657053B1 (en) 1999-02-26 2003-12-02 The Trustees Of Columbia University In The City Of New York Reciprocal subtraction differential display
WO1999043844A1 (en) * 1998-02-27 1999-09-02 The Trustees Of Columbia University In The City Of New York Reciprocal subtraction differential display
US5882874A (en) * 1998-02-27 1999-03-16 The Trustees Of Columbia University In The City Of New York Reciprocal subtraction differential display
US6090593A (en) * 1998-05-13 2000-07-18 The United States Of America As Represented By The Secretary Of The Air Force Isolation of expressed genes in microorganisms
DE19840731A1 (de) 1998-09-07 2000-03-09 Hoechst Marion Roussel De Gmbh Zwei Farben Differential Display als Verfahren zur Detektion regulierter Gene
AU6507099A (en) * 1998-09-29 2000-04-17 Arch Development Corporation A new strategy for genome-wide gene analysis: integrated procedures for gene identification
IL142965A0 (en) * 1998-11-04 2002-04-21 Digital Gene Tech Inc METHOD FOR INDEXING AND DETERMINING THE RELATIVE CONCENTRATION OF EXPRESSED MESSSENGER RNAs
AU1724000A (en) 1998-11-12 2000-05-29 Nyxis, Inc. Diagnostic assay for cancer
WO2000029621A2 (en) * 1998-11-16 2000-05-25 Genelabs Technologies, Inc. Method for measuring target polynucleotides and novel asthma biomolecules
US6156515A (en) * 1999-02-09 2000-12-05 Urocor, Inc. Prostate-specific gene for diagnosis, prognosis and management of prostate cancer
CN1384881A (zh) * 1999-02-19 2002-12-11 阿瑟西斯公司 非靶向活化内源基因的组合物和方法
AU3919900A (en) * 1999-03-25 2000-10-09 University Of Maryland Biotechnology Institute Method of differential display of prokaryotic messenger rna by rtpcr
US6759195B1 (en) 1999-03-25 2004-07-06 University Of Maryland Biotechnology Institute Method of differential display of prokaryotic messenger RNA by RTPCR
US6582906B1 (en) * 1999-04-05 2003-06-24 Affymetrix, Inc. Proportional amplification of nucleic acids
AU4471600A (en) * 1999-04-20 2000-11-02 Joslin Diabetes Center Inc. Disease reversing targets
EP1175501A4 (de) * 1999-05-12 2002-11-27 Invitrogen Corp Zusammensetzungen und verfahren zur erhöhung der empfindlichkeit und spezifität der nukleinsäuresynthese
US6429357B1 (en) 1999-05-14 2002-08-06 Dekalb Genetics Corp. Rice actin 2 promoter and intron and methods for use thereof
WO2000075356A1 (en) * 1999-06-04 2000-12-14 Lin Shi Lung Rna polymerase chain reaction
DE60042206D1 (de) * 1999-06-14 2009-06-25 New York Blood Ct Inc Die familie der camello-gene und ihre verwendungen
US6692915B1 (en) 1999-07-22 2004-02-17 Girish N. Nallur Sequencing a polynucleotide on a generic chip
US6864050B2 (en) 1999-07-30 2005-03-08 Affymetrix, Inc. Single-phase amplification of nucleic acids
US6416956B1 (en) 1999-08-13 2002-07-09 George Washington University Transcription factor, BP1
US6692918B2 (en) * 1999-09-13 2004-02-17 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
WO2001055454A1 (en) 2000-01-28 2001-08-02 Althea Technologies, Inc. Methods for analysis of gene expression
WO2001071036A2 (en) * 2000-03-17 2001-09-27 Gene Logic, Inc. Methods of preparing amplified nucleic acid molecules
US6468749B1 (en) 2000-03-30 2002-10-22 Quark Biotech, Inc. Sequence-dependent gene sorting techniques
AU2001251115A1 (en) * 2000-03-31 2001-10-15 Trustees Of Boston University Use of locally applied dna fragments
US6387624B1 (en) 2000-04-14 2002-05-14 Incyte Pharmaceuticals, Inc. Construction of uni-directionally cloned cDNA libraries from messenger RNA for improved 3′ end DNA sequencing
US7846733B2 (en) 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
ATE455186T1 (de) * 2000-06-26 2010-01-15 Nugen Technologies Inc Methoden und zusammensetzungen zur auf transkription basierenden vervielfältigung von nukleinsäuren
WO2002028876A2 (en) * 2000-10-05 2002-04-11 Riken Oligonucleotide linkers comprising a variable cohesive portion and method for the preparation of polynucleotide libraries by using said linkers.
CA2431170C (en) * 2000-12-12 2011-07-26 Aisin Seiki Kabushiki Kaisha Method of analyzing expression of gene
WO2002048402A2 (en) 2000-12-13 2002-06-20 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
US6794141B2 (en) 2000-12-22 2004-09-21 Arcturus Bioscience, Inc. Nucleic acid amplification
KR20030082535A (ko) 2001-03-09 2003-10-22 뉴젠 테크놀로지스 인코포레이티드 Ｒｎａ 서열의 증폭을 위한 방법 및 조성물
CA2439074A1 (en) * 2001-03-09 2002-09-19 Nugen Technologies, Inc. Methods and compositions for amplification of rna sequences
EP1275734A1 (de) * 2001-07-11 2003-01-15 Roche Diagnostics GmbH Verfahren zur zufällige Synthese und Amplifikation komplementäres DNA
EP1275738A1 (de) * 2001-07-11 2003-01-15 Roche Diagnostics GmbH Verfahren zur zufällige Synthese und Amplifikation komplementäres DNA
DE10143106C1 (de) * 2001-09-03 2002-10-10 Artus Ges Fuer Molekularbiolog Vermehrung von Ribonukleinsäuren
US20120077196A9 (en) * 2001-09-03 2012-03-29 Guido Krupp Universal method for selective amplification of mRNAs
JP4323317B2 (ja) * 2001-12-21 2009-09-02 フラームス・インテルウニフェルシタイル・インステイチュート・フォール・ビオテヒノロヒー・ヴェーゼットウェー（ヴェーイーベー・ヴェーゼットウェー） 可変領域配列のクローニング方法
AU2003222269A1 (en) * 2002-03-11 2003-09-29 Nugen Technologies, Inc. Methods for generating double stranded dna comprising a 3' single stranded portion and uses of these complexes for recombination
CA2486283A1 (en) * 2002-05-17 2004-02-05 Nugen Technologies, Inc. Methods for fragmentation, labeling and immobilization of nucleic acids
DE10240868A1 (de) * 2002-09-04 2004-03-18 Artus Gesellschaft für molekularbiologische Diagnostik und Entwicklung mbH Verbesserte Verfahren zur Synthese von Nukleinsäuren
US20060269924A1 (en) * 2003-04-11 2006-11-30 Trustees Of Boston University Modulation of telomere-initiated cell signaling
JP2006523465A (ja) 2003-04-14 2006-10-19 ニューゲン テクノロジーズ， インコーポレイテッド ランダムにプライミングされる複合プライマーを用いる大規模増幅
CA2552007A1 (en) * 2003-12-29 2005-07-21 Nugen Technologies, Inc. Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids
WO2005070948A1 (en) 2004-01-23 2005-08-04 Intronn, Inc. Correction of alpha-1-antitrypsin genetic defects using spliceosome mediated rna trans splicing
EP2290073A3 (de) 2004-05-28 2011-08-31 Asuragen, Inc. Verfahren und Zusammensetzungen mit MicroRNA
US20090197249A1 (en) 2004-11-01 2009-08-06 George Mason University Compositions and methods for diagnosing colon disorders
WO2006048262A2 (en) * 2004-11-04 2006-05-11 Roche Diagnostics Gmbh Classification of acute myeloid leukemia
CA2857878A1 (en) 2004-11-12 2006-12-28 Asuragen, Inc. Methods and compositions involving mirna and mirna inhibitor molecules
US7939258B2 (en) 2005-09-07 2011-05-10 Nugen Technologies, Inc. Nucleic acid amplification procedure using RNA and DNA composite primers
US7368246B2 (en) * 2005-09-15 2008-05-06 Primera Biosystems, Inc. Quantitative gene expression profiling
AT502823B1 (de) 2005-11-29 2007-06-15 Seitz Alexander Dr Polynukleotid-amplifikation
US7833716B2 (en) 2006-06-06 2010-11-16 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
EP2038429B1 (de) * 2006-06-30 2013-08-21 Nugen Technologies, Inc. Verfahren zur fragmentierung und etikettierung von nukleinsäuren
JP2010510964A (ja) 2006-09-19 2010-04-08 アシュラジェン インコーポレイテッド 治療的介入の標的としての、ｍｉＲ−１５、ｍｉＲ−２６、ｍｉＲ−３１、ｍｉＲ−１４５、ｍｉＲ−１４７、ｍｉＲ−１８８、ｍｉＲ−２１５、ｍｉＲ−２１６、ｍｉＲ−３３１、ｍｍｕ−ｍｉＲ−２９２−３ｐによって調節される遺伝子および経路
WO2008036765A2 (en) 2006-09-19 2008-03-27 Asuragen, Inc. Micrornas differentially expressed in pancreatic diseases and uses thereof
CA2672951A1 (en) 2006-12-20 2008-07-03 Bayer Healthcare Llc Hydroxy methyl phenyl pyrazolyl urea compound useful in the treatment of cancer
US8034568B2 (en) 2008-02-12 2011-10-11 Nugen Technologies, Inc. Isothermal nucleic acid amplification methods and compositions
GB2470672B (en) 2008-03-21 2012-09-12 Nugen Technologies Inc Methods of RNA amplification in the presence of DNA
US8258111B2 (en) 2008-05-08 2012-09-04 The Johns Hopkins University Compositions and methods related to miRNA modulation of neovascularization or angiogenesis
WO2010028014A1 (en) * 2008-09-03 2010-03-11 Abbott Laboratories Assays and kits for determining hiv-1 tropism
US8362318B2 (en) 2008-12-18 2013-01-29 Board Of Trustees Of Michigan State University Enzyme directed oil biosynthesis in microalgae
JP5749656B2 (ja) * 2009-02-02 2015-07-15 エクシコン エ／エス 低分子ｒｎａ種の定量化のための方法
EP2437592A4 (de) 2009-06-05 2013-02-27 Univ Florida Isolation und gezielte unterdrückung biosynthetischer lignin-gene aus zuckerrohr
US20110059453A1 (en) * 2009-08-23 2011-03-10 Affymetrix, Inc. Poly(A) Tail Length Measurement by PCR
DE102009056729A1 (de) * 2009-12-04 2011-06-09 Qiagen Gmbh Verfahren zum Nachweis und zur Quantifizierung von Poly(A)RNA und mRNA
WO2011082253A2 (en) 2009-12-30 2011-07-07 Board Of Trustees Of Michigan State University A method to produce acetyldiacylglycerols (ac-tags) by expression ofan acetyltransferase gene isolated from euonymus alatus (burning bush)
EP3214174B1 (de) 2010-03-04 2019-10-16 InteRNA Technologies B.V. Mirna-molekül, das über seine quelle definiert ist, und seine diagnostischen und therapeutischen verwendungen bei mit emt assoziierten krankheiten
WO2011130728A1 (en) 2010-04-17 2011-10-20 Bayer Healthcare Llc Synthetic metabolites of fluoro substituted omega-carboxyaryl diphenyl urea for the treatment and prevention diseases and conditions
NZ605420A (en) 2010-07-06 2015-02-27 Interna Technologies Bv Mirna and its diagnostic and therapeutic uses in diseases or conditions associated with melanoma, or in diseases or conditions associated with activated braf pathway
CA2817882C (en) 2010-11-17 2022-08-23 Asuragen, Inc. Mirnas as biomarkers for distinguishing benign from malignant thyroid neoplasms
EP2643045A4 (de) 2010-11-23 2016-01-13 Presage Biosciences Inc Therapeutische verfahren und zusammensetzungen zur abgabe in fester form
EP2474617A1 (de) 2011-01-11 2012-07-11 InteRNA Technologies BV MIR zur Behandlung von Neoangiogenese
MX2013009863A (es) 2011-02-28 2013-12-06 Univ Iowa Res Found Cambios de la hormona anti-mulleriana en el embarazo y prediccion de sexo y de resultados adversos en el embarazo.
WO2013040251A2 (en) 2011-09-13 2013-03-21 Asurgen, Inc. Methods and compositions involving mir-135b for distinguishing pancreatic cancer from benign pancreatic disease
SG10201510189WA (en) 2011-10-19 2016-01-28 Nugen Technologies Inc Compositions And Methods For Directional Nucleic Acid Amplification And Sequencing
US20130157884A1 (en) 2011-10-26 2013-06-20 Asuragen, Inc. Methods and compositions involving mirna expression levels for distinguishing pancreatic cysts
EP2771487A1 (de) 2011-10-27 2014-09-03 Asuragen, INC. Mirnas als diagnostische biomarker zur unterscheidung gutartiger von bösartigen tumoren in der schilddrüse
US9243037B2 (en) 2011-11-10 2016-01-26 Trustees Of Boston College Gramicidin a mutants that function as antibiotics with improved solubility and reduced toxicity
SG11201404243WA (en) 2012-01-26 2014-08-28 Nugen Technologies Inc Compositions and methods for targeted nucleic acid sequence enrichment and high efficiency library generation
JP6181751B2 (ja) 2012-06-18 2017-08-16 ニューゲン テクノロジーズ， インコーポレイテッド 望まれない核酸配列のネガティブ選択のための組成物および方法
US20150152499A1 (en) 2012-07-03 2015-06-04 Interna Technologies B.V. Diagnostic portfolio and its uses
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
US20140100124A1 (en) 2012-10-04 2014-04-10 Asuragen, Inc. Diagnostic mirnas for differential diagnosis of incidental pancreatic cystic lesions
WO2014144092A1 (en) 2013-03-15 2014-09-18 Nugen Technologies, Inc. Sequential sequencing
US9758835B2 (en) 2013-03-15 2017-09-12 Baylor Research Institute Ulcerative colitis (UC)-associated colorectal neoplasia markers
US9868992B2 (en) 2013-03-15 2018-01-16 Baylor Research Institute Tissue and blood-based miRNA biomarkers for the diagnosis, prognosis and metastasis-predictive potential in colorectal cancer
US9944992B2 (en) 2013-03-15 2018-04-17 The University Of Chicago Methods and compositions related to T-cell activity
CA2929596C (en) 2013-11-13 2022-07-05 Nugen Technologies, Inc. Compositions and methods for identification of a duplicate sequencing read
US10392629B2 (en) 2014-01-17 2019-08-27 Board Of Trustees Of Michigan State University Increased caloric and nutritional content of plant biomass
US9745614B2 (en) 2014-02-28 2017-08-29 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
US10550440B2 (en) 2016-02-26 2020-02-04 Arizona Board Of Regents On Behalf Of Arizona State University Synthetic translation-sensing riboswitches and uses thereof
US12492430B2 (en) 2017-04-11 2025-12-09 Tecan Genomics, Inc. Library quantitation and qualification
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
EP3704250A1 (de) 2017-11-03 2020-09-09 InteRNA Technologies B.V. Mirna-molekül, äquivalent, antagomir oder quelle davon zur behandlung und/oder diagnose eines zustands und/oder einer erkrankung im zusammenhang mit neuronaler defizienz oder neuronaler (re)generation
EP3714053B1 (de) 2017-11-22 2025-12-31 The University of Chicago Von chemischer sonde abhängige bewertung der proteinaktivität und verwendung davon
US20220205035A1 (en) 2019-04-05 2022-06-30 Board Of Regents, The University Of Texas System Methods and applications for cell barcoding
WO2020210521A2 (en) 2019-04-12 2020-10-15 The Regents Of The University Of California Compositions and methods for increasing muscle mass and oxidative metabolism
ES3015628T3 (en) * 2019-08-20 2025-05-06 Director General Of Nat Institute Of Infectious Diseases Method for amplifying nucleotide sequence, and sequence determination method
EP3851542A1 (de) 2020-01-20 2021-07-21 Tecan Genomics, Inc. Verarmung von reichlich uninformativen sequenzen
CN113275053A (zh) 2020-02-03 2021-08-20 帝肯基因组学公司 试剂存储系统
WO2024028794A1 (en) 2022-08-02 2024-02-08 Temple Therapeutics BV Methods for treating endometrial and ovarian hyperproliferative disorders
WO2025248505A1 (en) 2024-05-31 2025-12-04 Wayne State University Methods for treating endometrial and ovarian hyperproliferative disorders

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
CA1338457C (en) * 1986-08-22 1996-07-16 Henry A. Erlich Purified thermostable enzyme
US5066584A (en) * 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
US5104792A (en) * 1989-12-21 1992-04-14 The United States Of America As Represented By The Department Of Health And Human Services Method for amplifying unknown nucleic acid sequences
CA2086015A1 (en) * 1990-06-20 1991-12-21 Jayne S. Danska Identification of cell subpopulations using modified pcr to amplify expression intermediates
WO1993014217A1 (en) * 1992-01-10 1993-07-22 Life Technologies, Inc. Use of predetermined nucleotides having altered base pairing characteristics in the amplification of nucleic acid molecules
US5262311A (en) * 1992-03-11 1993-11-16 Dana-Farber Cancer Institute, Inc. Methods to clone polyA mRNA
DE4317414C1 (de) * 1993-05-18 1994-04-21 Max Planck Gesellschaft Mittel zur komplexen Diagnostik der Genexpression und Verfahren zur Anwendung für die medizinische Diagnostik und die Genisolierung

Also Published As

Publication number Publication date
US5965409A (en) 1999-10-12
EP0592626B1 (de) 2003-01-29
US5599672A (en) 1997-02-04
EP0592626A4 (en) 1994-08-17
JPH07500735A (ja) 1995-01-26
DE69332665D1 (de) 2003-03-06
CA2102784A1 (en) 1993-09-12
EP0592626A1 (de) 1994-04-20
DE69332665T2 (de) 2003-11-27
WO1993018176A1 (en) 1993-09-16
JP2843675B2 (ja) 1999-01-06
US5665547A (en) 1997-09-09

Similar Documents

Publication Publication Date Title
DE69332665D1 (de) Methode um mrna zu klonieren
CA2185239A1 (en) Isothermal strand displacement nucleic acid amplification
FR2724934B1 (fr) Oligonucleotide chimere et son utilisation dans l'obtention de transcrits d'un acide nucleique
WO1998005770A3 (de) Anti-sinn-rna mit sekundärstruktur
IE842668L (en) Method of inhibiting propagation of virus and anti-viral¹agent
EP0821059B1 (de) Verfahren für reverse Transkription
ES2169943T3 (es) Metodo para la amplificacion no especifica de acido nucleico.
WO2002044399A3 (en) In vitro transcription method for rna amplification
DK0730664T3 (da) Nucleinsyreanaloginduceret transkription af dobbeltstrenget DNA
DK1300466T3 (da) En kaskade nukleinsyre amplifikationsreaktion
WO2002006533A3 (en) Single primer pcr amplification of rna
ATE428800T1 (de) Verfahren zur klonierung von cdna mit voller länge
GR3024597T3 (en) 7-deazapurine modified oligonucleotides
ES2108748T3 (es) Amplificacion de acidos nucleicos con actividad de rna polimerasa dependiente de dna, de rna replicasas.
DK153289A (da) Selektiv forstaerkning af maal-polynucleotidsekvenser
AU1272000A (en) Method for the quantitative detection of nucleic acids
DE69427876D1 (de) Methode zur detektion einer gezielten nukleinsäure
IL145588A0 (en) Amplification and sequencing primer pairs and use thereof
FI962087L (fi) Olefiinien polymerointiin tarkoitettu stereospesifinen katalyyttisysteemi ja monivaiheiset polymerointiprosessit, joissa tätä systeemiä käytetään
AU5395196A (en) Primers and methods for simultaneous amplification of multip le markers for dna fingerprinting
HUP9902152A2 (hu) Gyógyszerészeti minőségű plazmid DNS tisztítása
WO1995027047A3 (en) Highly-purified recombinant reverse transcriptase
WO1994013789A3 (en) Oligonucleotides with rna cleavage activity
EP0255485A3 (de) Transdermale Mittel
Liang et al. Method of differential display of exposed MRNA by RT/PCR

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties