ATE231920T1 - Methode um mrna zu klonieren - Google Patents

Methode um mrna zu klonieren

Info

Publication number
ATE231920T1
ATE231920T1 AT93907440T AT93907440T ATE231920T1 AT E231920 T1 ATE231920 T1 AT E231920T1 AT 93907440 T AT93907440 T AT 93907440T AT 93907440 T AT93907440 T AT 93907440T AT E231920 T1 ATE231920 T1 AT E231920T1
Authority
AT
Austria
Prior art keywords
sequence
primer contains
primer
mrna
another approach
Prior art date
Application number
AT93907440T
Other languages
English (en)
Inventor
Peng Liang
Arthur B Pardee
Cesario F Bianchi
Original Assignee
Dana Farber Cancer Inst Inc
Harvard College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US07/850,343 external-priority patent/US5262311A/en
Application filed by Dana Farber Cancer Inst Inc, Harvard College filed Critical Dana Farber Cancer Inst Inc
Application granted granted Critical
Publication of ATE231920T1 publication Critical patent/ATE231920T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
AT93907440T 1992-03-11 1993-03-11 Methode um mrna zu klonieren ATE231920T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US07/850,343 US5262311A (en) 1992-03-11 1992-03-11 Methods to clone polyA mRNA
US3308493A 1993-03-11 1993-03-11
PCT/US1993/002246 WO1993018176A1 (en) 1992-03-11 1993-03-11 METHODS TO CLONE mRNA

Publications (1)

Publication Number Publication Date
ATE231920T1 true ATE231920T1 (de) 2003-02-15

Family

ID=26709258

Family Applications (1)

Application Number Title Priority Date Filing Date
AT93907440T ATE231920T1 (de) 1992-03-11 1993-03-11 Methode um mrna zu klonieren

Country Status (7)

Country Link
US (3) US5599672A (de)
EP (1) EP0592626B1 (de)
JP (1) JP2843675B2 (de)
AT (1) ATE231920T1 (de)
CA (1) CA2102784A1 (de)
DE (1) DE69332665T2 (de)
WO (1) WO1993018176A1 (de)

Families Citing this family (154)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7049102B1 (en) 1989-09-22 2006-05-23 Board Of Trustees Of Leland Stanford University Multi-gene expression profile
US5545522A (en) 1989-09-22 1996-08-13 Van Gelder; Russell N. Process for amplifying a target polynucleotide sequence using a single primer-promoter complex
US5795714A (en) 1992-11-06 1998-08-18 Trustees Of Boston University Method for replicating an array of nucleic acid probes
US5744300A (en) * 1993-03-24 1998-04-28 Geron Corporation Methods and reagents for the identification and regulation of senescence-related genes
US5580726A (en) * 1994-04-29 1996-12-03 Geron Corporation Method and Kit for enhanced differential display
DE4317414C1 (de) * 1993-05-18 1994-04-21 Max Planck Gesellschaft Mittel zur komplexen Diagnostik der Genexpression und Verfahren zur Anwendung für die medizinische Diagnostik und die Genisolierung
WO1995011923A1 (en) * 1993-10-29 1995-05-04 Dana-Farber Cancer Institute, Inc. A novel tumor marker and novel method of isolating same
US5459037A (en) * 1993-11-12 1995-10-17 The Scripps Research Institute Method for simultaneous identification of differentially expressed mRNAs and measurement of relative concentrations
US6096503A (en) * 1993-11-12 2000-08-01 The Scripps Research Institute Method for simultaneous identification of differentially expresses mRNAs and measurement of relative concentrations
US6110680A (en) * 1993-11-12 2000-08-29 The Scripps Research Institute Method for simultaneous identification of differentially expressed mRNAs and measurement of relative concentrations
US20050123926A1 (en) * 1994-01-13 2005-06-09 Enzo Diagnostics, Inc., In vitro process for producing multiple nucleic acid copies
US20110097791A1 (en) * 1999-04-16 2011-04-28 Engelhardt Dean L Novel process, construct and conjugate for producing multiple nucleic acid copies
US6986985B1 (en) * 1994-01-13 2006-01-17 Enzo Life Sciences, Inc. Process for producing multiple nucleic acid copies in vivo using a protein-nucleic acid construct
US6379897B1 (en) * 2000-11-09 2002-04-30 Nanogen, Inc. Methods for gene expression monitoring on electronic microarrays
RU2111254C1 (ru) * 1994-07-11 1998-05-20 Институт молекулярной биологии им.В.А.Энгельгардта РАН СПОСОБ ДЕТЕКЦИИ ДИФФЕРЕНЦИАЛЬНО ЭКСПРЕССИРУЮЩИХСЯ МАТРИЧНЫХ РНК И КЛОНИРОВАНИЯ СООТВЕТСТВУЮЩИХ ИМ ФРАГМЕНТОВ кДНК
DE69621507T2 (de) * 1995-03-28 2003-01-09 Japan Science And Technology Corp., Kawaguchi Verfahren zur molekularen Indexierung von Genen unter Verwendung von Restriktionsenzymen
DE19518505A1 (de) * 1995-05-19 1996-11-21 Max Planck Gesellschaft Verfahren zur Genexpressionsanalyse
US7094766B1 (en) 1995-06-06 2006-08-22 Trustees Of Boston University Use of locally applied DNA fragments
US6147056A (en) * 1995-06-06 2000-11-14 Trustees Of Boston University Use of locally applied DNA fragments
WO2001074342A2 (en) * 2000-03-31 2001-10-11 Trustees Of Boston University Use of locally applied dna fragments
US20030032610A1 (en) * 1996-06-03 2003-02-13 Gilchrest Barbara A. Method to inhibit cell growth using oligonucleotides
EP0951541B1 (de) * 1995-07-31 2005-11-30 Urocor, Inc. Biomarker und ziele für die diagnose, prognose und handhabung von prostatkrebs
US6218529B1 (en) * 1995-07-31 2001-04-17 Urocor, Inc. Biomarkers and targets for diagnosis, prognosis and management of prostate, breast and bladder cancer
AU2264197A (en) 1996-02-09 1997-08-28 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The Restriction display (rd-pcr) of differentially expressed mrnas
WO1997037045A1 (en) * 1996-04-03 1997-10-09 Johnson & Johnson Consumer Products, Inc. Improved technique for differential display
US5776683A (en) * 1996-07-11 1998-07-07 California Pacific Medical Center Methods for identifying genes amplified in cancer cells
EP0811688A3 (de) * 1996-06-06 1998-01-14 Smithkline Beecham Corporation Staphylococcus aureus RNase P
CA2234736A1 (en) * 1996-07-16 1998-01-22 Periannan Senapathy Method for contiguous genome sequencing
JPH1057064A (ja) 1996-08-16 1998-03-03 Rikagaku Kenkyusho 微量遺伝子産物の特異的増幅方法
EP1007730A4 (de) * 1996-08-30 2004-04-28 Invitrogen Corp Verfahren zur identifizierung und isolierung von spezifischen nukleotidsequenzen in cdna und genomischer dna
US20040142327A1 (en) * 1996-11-22 2004-07-22 Jhy-Jhu Lin Methods for identifying and isolating DNA polymorphisms or genetic markers
US6306588B1 (en) * 1997-02-07 2001-10-23 Invitrogen Corporation Polymerases for analyzing or typing polymorphic nucleic acid fragments and uses thereof
AU6444698A (en) * 1997-03-03 1998-09-22 Tariq M. HAQQI Methods and compositions for identifying expressed genes
US6162616A (en) * 1997-04-16 2000-12-19 Millennium Pharmaceuticals, Inc. Multidrug resistance-associated polypeptide
AU7265298A (en) * 1997-04-29 1998-11-24 Trustees Of Boston University Methods and compositions for targeted dna differential display
EP0979282A1 (de) * 1997-04-30 2000-02-16 F. Hoffmann-La Roche Ag Rattenchemonikin st38.2
US6261770B1 (en) 1997-05-13 2001-07-17 Display Systems Biotech Aps Method to clone mRNAs
JP2001515362A (ja) * 1997-05-13 2001-09-18 ディスプレイ・システムズ・バイオテック・アクティーゼルスカブ ｍＲＮＡをクローニングする方法および差次的に発現された転写物のディスプレイ（ＤＯＤＥＴ）
US5994076A (en) * 1997-05-21 1999-11-30 Clontech Laboratories, Inc. Methods of assaying differential expression
US6489455B2 (en) 1997-05-21 2002-12-03 Clontech Laboratories, Inc. Methods of assaying differential expression
EP1007739A2 (de) * 1997-09-05 2000-06-14 Sidney Kimmel Cancer Center Auswahl von pcr primerpaaren zur amplifikation einer gruppe von nukleotidsequenzen
US7031843B1 (en) 1997-09-23 2006-04-18 Gene Logic Inc. Computer methods and systems for displaying information relating to gene expression data
US6150096A (en) * 1997-09-26 2000-11-21 Universite De Sherbrooke Molecular markers for the diagnosis of human diseases including Crohn's disease
US6897066B1 (en) 1997-09-26 2005-05-24 Athersys, Inc. Compositions and methods for non-targeted activation of endogenous genes
US6277571B1 (en) 1997-10-03 2001-08-21 Virginia Commonwealth University Intellectual Property Foundation Sequential consensus region-directed amplification of known and novel members of gene families
US6090553A (en) * 1997-10-29 2000-07-18 Beckman Coulter, Inc. Use of uracil-DNA glycosylase in genetic analysis
US6087102A (en) 1998-01-07 2000-07-11 Clontech Laboratories, Inc. Polymeric arrays and methods for their use in binding assays
DE19806431C1 (de) * 1998-02-17 1999-10-14 Novartis Ag Neues Verfahren zur Identifikation und Charakterisierung von mRNA-Molekülen
US6365346B1 (en) 1998-02-18 2002-04-02 Dade Behring Inc. Quantitative determination of nucleic acid amplification products
US5882874A (en) * 1998-02-27 1999-03-16 The Trustees Of Columbia University In The City Of New York Reciprocal subtraction differential display
US6657053B1 (en) 1999-02-26 2003-12-02 The Trustees Of Columbia University In The City Of New York Reciprocal subtraction differential display
WO1999043844A1 (en) * 1998-02-27 1999-09-02 The Trustees Of Columbia University In The City Of New York Reciprocal subtraction differential display
US6090593A (en) * 1998-05-13 2000-07-18 The United States Of America As Represented By The Secretary Of The Air Force Isolation of expressed genes in microorganisms
DE19840731A1 (de) * 1998-09-07 2000-03-09 Hoechst Marion Roussel De Gmbh Zwei Farben Differential Display als Verfahren zur Detektion regulierter Gene
WO2000018966A2 (en) * 1998-09-29 2000-04-06 Arch Development Corporation A new strategy for genome-wide gene analysis: integrated procedures for gene identification
EA200100490A1 (ru) * 1998-11-04 2001-10-22 Диджитал Гене Текнолоджиз, Инк. Способ индексации и определения относительной концентрации экспрессирующихся информационных рнк
WO2000028090A2 (en) 1998-11-12 2000-05-18 Nyxis, Inc. Diagnostic assay for cancer
AU1478200A (en) * 1998-11-16 2000-06-05 Genelabs Technologies, Inc. Method for measuring target polynucleotides and novel asthma biomolecules
US6156515A (en) * 1999-02-09 2000-12-05 Urocor, Inc. Prostate-specific gene for diagnosis, prognosis and management of prostate cancer
WO2000049162A2 (en) * 1999-02-19 2000-08-24 Athersys, Inc. Compositions and methods for non-targeted activation of endogenous genes
WO2000056936A1 (en) * 1999-03-25 2000-09-28 The University Of Maryland Biotechnology Institute Method of differential display of prokaryotic messenger rna by rtpcr
US6759195B1 (en) 1999-03-25 2004-07-06 University Of Maryland Biotechnology Institute Method of differential display of prokaryotic messenger RNA by RTPCR
US6582906B1 (en) * 1999-04-05 2003-06-24 Affymetrix, Inc. Proportional amplification of nucleic acids
AU4471600A (en) * 1999-04-20 2000-11-02 Joslin Diabetes Center Inc. Disease reversing targets
EP1175501A4 (de) * 1999-05-12 2002-11-27 Invitrogen Corp Zusammensetzungen und verfahren zur erhöhung der empfindlichkeit und spezifität der nukleinsäuresynthese
US6429357B1 (en) 1999-05-14 2002-08-06 Dekalb Genetics Corp. Rice actin 2 promoter and intron and methods for use thereof
WO2000075356A1 (en) * 1999-06-04 2000-12-14 Lin Shi Lung Rna polymerase chain reaction
US6607914B1 (en) * 1999-06-14 2003-08-19 New York Blood Center, Inc. Camello gene family and uses thereof
US6692915B1 (en) 1999-07-22 2004-02-17 Girish N. Nallur Sequencing a polynucleotide on a generic chip
US6864050B2 (en) 1999-07-30 2005-03-08 Affymetrix, Inc. Single-phase amplification of nucleic acids
US6416956B1 (en) 1999-08-13 2002-07-09 George Washington University Transcription factor, BP1
US6692918B2 (en) * 1999-09-13 2004-02-17 Nugen Technologies, Inc. Methods and compositions for linear isothermal amplification of polynucleotide sequences
CA2398107C (en) 2000-01-28 2013-11-19 Althea Technologies, Inc. Methods for analysis of gene expression
AU2001250858A1 (en) * 2000-03-17 2001-10-03 Gene Logic, Inc. Methods of preparing amplified nucleic acid molecules
US6468749B1 (en) 2000-03-30 2002-10-22 Quark Biotech, Inc. Sequence-dependent gene sorting techniques
US6387624B1 (en) 2000-04-14 2002-05-14 Incyte Pharmaceuticals, Inc. Construction of uni-directionally cloned cDNA libraries from messenger RNA for improved 3′ end DNA sequencing
US7846733B2 (en) 2000-06-26 2010-12-07 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
CA2412721A1 (en) * 2000-06-26 2002-01-03 Nugen Technologies, Inc. Methods and compositions for transcription-based nucleic acid amplification
JP4340779B2 (ja) * 2000-10-05 2009-10-07 独立行政法人理化学研究所 可変突出部位を含むオリゴヌクレオチドリンカー及び前記リンカーを用いたポリヌクレオチドライブラリーの調製方法
AU2261802A (en) * 2000-12-12 2002-06-24 Masumi Abe Method of analyzing gene expression
CA2430329A1 (en) 2000-12-13 2002-06-20 Nugen Technologies, Inc. Methods and compositions for generation of multiple copies of nucleic acid sequences and methods of detection thereof
US6794141B2 (en) 2000-12-22 2004-09-21 Arcturus Bioscience, Inc. Nucleic acid amplification
US7094536B2 (en) * 2001-03-09 2006-08-22 Nugen Technologies, Inc. Methods and compositions for amplification of RNA sequences
MXPA02012739A (es) 2001-03-09 2004-04-20 Nugen Technologies Inc Metodos y composiciones para amplificacion de secuencias de arn.
EP1275738A1 (de) * 2001-07-11 2003-01-15 Roche Diagnostics GmbH Verfahren zur zufällige Synthese und Amplifikation komplementäres DNA
EP1275734A1 (de) * 2001-07-11 2003-01-15 Roche Diagnostics GmbH Verfahren zur zufällige Synthese und Amplifikation komplementäres DNA
US20120077196A9 (en) * 2001-09-03 2012-03-29 Guido Krupp Universal method for selective amplification of mRNAs
DE10143106C1 (de) * 2001-09-03 2002-10-10 Artus Ges Fuer Molekularbiolog Vermehrung von Ribonukleinsäuren
EP1456237A2 (de) * 2001-12-21 2004-09-15 Vlaams Interuniversitair Instituut voor Biotechnologie vzw. Methode zur klonierung von sequenzen aus den variablen domänen
JP2005519621A (ja) * 2002-03-11 2005-07-07 ニューゲン テクノロジーズ， インコーポレイテッド ３’一本鎖部分を含む二本鎖ｄｎａを生成する方法および組換えのためのこれらの複合体の使用
AU2003279697A1 (en) * 2002-05-17 2004-02-16 Nugen Technologies, Inc. Methods for fragmentation, labeling and immobilizaton of nucleic acids
DE10240868A1 (de) * 2002-09-04 2004-03-18 Artus Gesellschaft für molekularbiologische Diagnostik und Entwicklung mbH Verbesserte Verfahren zur Synthese von Nukleinsäuren
US20060269924A1 (en) * 2003-04-11 2006-11-30 Trustees Of Boston University Modulation of telomere-initiated cell signaling
US7402386B2 (en) 2003-04-14 2008-07-22 Nugen Technologies, Inc. Global amplification using random priming by a composite primer
WO2005065321A2 (en) * 2003-12-29 2005-07-21 Nugen Technologies, Inc. Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids
US8053232B2 (en) 2004-01-23 2011-11-08 Virxsys Corporation Correction of alpha-1-antitrypsin genetic defects using spliceosome mediated RNA trans splicing
EP2290073A3 (de) 2004-05-28 2011-08-31 Asuragen, Inc. Verfahren und Zusammensetzungen mit MicroRNA
US20090197249A1 (en) 2004-11-01 2009-08-06 George Mason University Compositions and methods for diagnosing colon disorders
WO2006048262A2 (en) * 2004-11-04 2006-05-11 Roche Diagnostics Gmbh Classification of acute myeloid leukemia
EP2302055B1 (de) 2004-11-12 2014-08-27 Asuragen, Inc. Verfahren und Zusammensetzungen, die miRNAs und miRNAa-inhibitorischen Molekülen verbunden sind
CA2621267A1 (en) 2005-09-07 2007-03-15 Nugen Technologies, Inc. Improved nucleic acid amplification procedure
US7368246B2 (en) * 2005-09-15 2008-05-06 Primera Biosystems, Inc. Quantitative gene expression profiling
AT502823B1 (de) 2005-11-29 2007-06-15 Seitz Alexander Dr Polynukleotid-amplifikation
US7833716B2 (en) 2006-06-06 2010-11-16 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
US20100022403A1 (en) * 2006-06-30 2010-01-28 Nurith Kurn Methods for fragmentation and labeling of nucleic acids
US20090131348A1 (en) 2006-09-19 2009-05-21 Emmanuel Labourier Micrornas differentially expressed in pancreatic diseases and uses thereof
WO2008036776A2 (en) 2006-09-19 2008-03-27 Asuragen, Inc. Mir-15, mir-26, mir -31,mir -145, mir-147, mir-188, mir-215, mir-216 mir-331, mmu-mir-292-3p regulated genes and pathways as targets for therapeutic intervention
JP2010514692A (ja) 2006-12-20 2010-05-06 バイエル ヘルスケア リミティド ライアビリティ カンパニー 癌の治療に有用なヒドロキシメチルフェニルピラゾリル尿素化合物
US8034568B2 (en) 2008-02-12 2011-10-11 Nugen Technologies, Inc. Isothermal nucleic acid amplification methods and compositions
WO2009117698A2 (en) 2008-03-21 2009-09-24 Nugen Technologies, Inc. Methods of rna amplification in the presence of dna
WO2009137807A2 (en) 2008-05-08 2009-11-12 Asuragen, Inc. Compositions and methods related to mirna modulation of neovascularization or angiogenesis
EP2326957B1 (de) * 2008-09-03 2013-06-12 Abbott Molecular Inc. Tests und kits zur bestimmung von hiv-1-tropismus
US8362318B2 (en) 2008-12-18 2013-01-29 Board Of Trustees Of Michigan State University Enzyme directed oil biosynthesis in microalgae
DK2391736T3 (en) * 2009-02-02 2015-05-11 Exiqon As A method for the quantitation of small RNA species
AU2010256356B2 (en) 2009-06-05 2015-07-16 University Of Florida Research Foundation, Inc. Isolation and targeted suppression of lignin biosynthetic genes from sugarcane
US20110059453A1 (en) * 2009-08-23 2011-03-10 Affymetrix, Inc. Poly(A) Tail Length Measurement by PCR
DE102009056729A1 (de) * 2009-12-04 2011-06-09 Qiagen Gmbh Verfahren zum Nachweis und zur Quantifizierung von Poly(A)RNA und mRNA
WO2011082253A2 (en) 2009-12-30 2011-07-07 Board Of Trustees Of Michigan State University A method to produce acetyldiacylglycerols (ac-tags) by expression ofan acetyltransferase gene isolated from euonymus alatus (burning bush)
EP2542678B1 (de) 2010-03-04 2017-04-12 InteRNA Technologies B.V. Mirna-molekül, das durch seine quelle definiert ist, und seine therapeutischen verwendungen bei emt assoziierten krebs
CN103079567A (zh) 2010-04-17 2013-05-01 拜尔健康护理有限责任公司 用于疾病和病症的治疗和预防的氟取代的ω-羧基芳基二苯脲的合成代谢产物
EP2591106A1 (de) 2010-07-06 2013-05-15 InteRNA Technologies B.V. Mirna und ihre diagnostischen und therapeutischen verwendungen für mit melanomen assoziierte erkrankungen oder leiden oder für mit dem aktivierten braf-pfad assoziierte erkrankungen oder leiden
BR112013012265A2 (pt) 2010-11-17 2016-08-02 Asuragen Inc mirnas como biomarcadores para distinguir neoplasmas de tireoide benignos de malignos
WO2012071559A2 (en) 2010-11-23 2012-05-31 Presage Biosciences, Inc. Therapeutic methods and compositions for solid delivery
EP2474617A1 (de) 2011-01-11 2012-07-11 InteRNA Technologies BV MIR zur Behandlung von Neoangiogenese
EP2681566A2 (de) 2011-02-28 2014-01-08 University of Iowa Research Foundation Anti-müller-hormon-veränderungen in der schwangerschaft und vorhersage von schwangerschaftsnebenwirkungen und des geschlechts
WO2013040251A2 (en) 2011-09-13 2013-03-21 Asurgen, Inc. Methods and compositions involving mir-135b for distinguishing pancreatic cancer from benign pancreatic disease
WO2013059746A1 (en) 2011-10-19 2013-04-25 Nugen Technologies, Inc. Compositions and methods for directional nucleic acid amplification and sequencing
WO2013063519A1 (en) 2011-10-26 2013-05-02 Asuragen, Inc. Methods and compositions involving mirna expression levels for distinguishing pancreatic cysts
WO2013063544A1 (en) 2011-10-27 2013-05-02 Asuragen, Inc. Mirnas as diagnostic biomarkers to distinguish benign from malignant thyroid tumors
WO2013071049A1 (en) 2011-11-10 2013-05-16 Trustees Of Boston College Gramicidin a mutants that function as antibiotics with improved solubility and reduced toxicity
EP2807292B1 (de) 2012-01-26 2019-05-22 Tecan Genomics, Inc. Zusammensetzungen und verfahren zur gezielten nukleinsäuresequenzanreicherung und erzeugung einer hocheffizienten bibliothek
CA2877094A1 (en) 2012-06-18 2013-12-27 Nugen Technologies, Inc. Compositions and methods for negative selection of non-desired nucleic acid sequences
WO2014007623A1 (en) 2012-07-03 2014-01-09 Interna Technologies B.V. Diagnostic portfolio and its uses
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
WO2014055117A1 (en) 2012-10-04 2014-04-10 Asuragen, Inc. Diagnostic mirnas for differential diagnosis of incidental pancreatic cystic lesions
CA2907377A1 (en) 2013-03-15 2014-09-18 Baylor Research Institute Tissue and blood-based mirna biomarkers for the diagnosis, prognosis and metastasis-predictive potential in colorectal cancer
US9944992B2 (en) 2013-03-15 2018-04-17 The University Of Chicago Methods and compositions related to T-cell activity
US9822408B2 (en) 2013-03-15 2017-11-21 Nugen Technologies, Inc. Sequential sequencing
US9758835B2 (en) 2013-03-15 2017-09-12 Baylor Research Institute Ulcerative colitis (UC)-associated colorectal neoplasia markers
WO2015073711A1 (en) 2013-11-13 2015-05-21 Nugen Technologies, Inc. Compositions and methods for identification of a duplicate sequencing read
US10392629B2 (en) 2014-01-17 2019-08-27 Board Of Trustees Of Michigan State University Increased caloric and nutritional content of plant biomass
US9745614B2 (en) 2014-02-28 2017-08-29 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
US10550440B2 (en) 2016-02-26 2020-02-04 Arizona Board Of Regents On Behalf Of Arizona State University Synthetic translation-sensing riboswitches and uses thereof
US12492430B2 (en) 2017-04-11 2025-12-09 Tecan Genomics, Inc. Library quantitation and qualification
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
CN111566212A (zh) 2017-11-03 2020-08-21 因特尔纳技术有限公司 miRNA分子，等同物，安塔够妙或其来源用于治疗和/或诊断与神经元缺陷相关的病症和/或疾病或用于神经元生成和/或再生
WO2019104155A2 (en) 2017-11-22 2019-05-31 The University Of Chicago Chemical probe-dependent evaluation of protein activity and uses thereof
EP3947727A4 (de) 2019-04-05 2023-01-04 Board of Regents, The University of Texas System Verfahren und anwendungen zur zellen-barcodierung
JP7566775B2 (ja) 2019-04-12 2024-10-15 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 筋肉量及び酸化的代謝を増加させるための組成物及び方法
JP7426032B2 (ja) * 2019-08-20 2024-02-01 国立感染症研究所長 ヌクレオチド配列の増幅方法及び配列決定方法
EP3851542A1 (de) 2020-01-20 2021-07-21 Tecan Genomics, Inc. Verarmung von reichlich uninformativen sequenzen
US12059674B2 (en) 2020-02-03 2024-08-13 Tecan Genomics, Inc. Reagent storage system
WO2024028794A1 (en) 2022-08-02 2024-02-08 Temple Therapeutics BV Methods for treating endometrial and ovarian hyperproliferative disorders
WO2025248505A1 (en) 2024-05-31 2025-12-04 Wayne State University Methods for treating endometrial and ovarian hyperproliferative disorders

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
CA1338457C (en) * 1986-08-22 1996-07-16 Henry A. Erlich Purified thermostable enzyme
US5066584A (en) * 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
US5104792A (en) * 1989-12-21 1992-04-14 The United States Of America As Represented By The Department Of Health And Human Services Method for amplifying unknown nucleic acid sequences
CA2086015A1 (en) * 1990-06-20 1991-12-21 Jayne S. Danska Identification of cell subpopulations using modified pcr to amplify expression intermediates
WO1993014217A1 (en) * 1992-01-10 1993-07-22 Life Technologies, Inc. Use of predetermined nucleotides having altered base pairing characteristics in the amplification of nucleic acid molecules
US5262311A (en) * 1992-03-11 1993-11-16 Dana-Farber Cancer Institute, Inc. Methods to clone polyA mRNA
DE4317414C1 (de) * 1993-05-18 1994-04-21 Max Planck Gesellschaft Mittel zur komplexen Diagnostik der Genexpression und Verfahren zur Anwendung für die medizinische Diagnostik und die Genisolierung

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WO1993018176A1 (en) 1993-09-16
DE69332665D1 (de) 2003-03-06
EP0592626B1 (de) 2003-01-29
JPH07500735A (ja) 1995-01-26
US5665547A (en) 1997-09-09
CA2102784A1 (en) 1993-09-12
US5965409A (en) 1999-10-12
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