ATE233314T1 - Eine sekretionssequenz für die herstellung von heterologen proteinen in hefe - Google Patents

Eine sekretionssequenz für die herstellung von heterologen proteinen in hefe

Info

Publication number
ATE233314T1
ATE233314T1 AT94926033T AT94926033T ATE233314T1 AT E233314 T1 ATE233314 T1 AT E233314T1 AT 94926033 T AT94926033 T AT 94926033T AT 94926033 T AT94926033 T AT 94926033T AT E233314 T1 ATE233314 T1 AT E233314T1
Authority
AT
Austria
Prior art keywords
yeast
sequence encoding
dna molecule
protein
heterologue
Prior art date
Application number
AT94926033T
Other languages
English (en)
Inventor
Richard W Scott
Russell Arthur Brierley
David Scott Howland
Original Assignee
Cephalon Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cephalon Inc filed Critical Cephalon Inc
Application granted granted Critical
Publication of ATE233314T1 publication Critical patent/ATE233314T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
AT94926033T 1993-09-16 1994-08-24 Eine sekretionssequenz für die herstellung von heterologen proteinen in hefe ATE233314T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/122,889 US5521086A (en) 1993-09-16 1993-09-16 Secretion sequence for the production of a heterologous protein in yeast
PCT/US1994/009653 WO1995007978A1 (en) 1993-09-16 1994-08-24 A secretion sequence for the production of a heterologous protein in yeast

Publications (1)

Publication Number Publication Date
ATE233314T1 true ATE233314T1 (de) 2003-03-15

Family

ID=22405437

Family Applications (1)

Application Number Title Priority Date Filing Date
AT94926033T ATE233314T1 (de) 1993-09-16 1994-08-24 Eine sekretionssequenz für die herstellung von heterologen proteinen in hefe

Country Status (9)

Country Link
US (1) US5521086A (de)
EP (1) EP0728191B1 (de)
JP (1) JPH09502609A (de)
AT (1) ATE233314T1 (de)
AU (1) AU689496B2 (de)
DE (1) DE69432185T2 (de)
FI (1) FI961238A7 (de)
NZ (1) NZ271928A (de)
WO (1) WO1995007978A1 (de)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3486216T2 (de) * 1983-04-25 1994-02-17 Chiron Corp Hybrid-DNS-Synthesis von reifen insulinähnlichen Wachstumsfaktoren.
FR2686899B1 (fr) * 1992-01-31 1995-09-01 Rhone Poulenc Rorer Sa Nouveaux polypeptides biologiquement actifs, leur preparation et compositions pharmaceutiques les contenant.
WO1996007744A1 (en) * 1994-09-08 1996-03-14 Chiron Corporation A method of improved production of insulin-like growth factor
EP1003889A1 (de) 1997-08-05 2000-05-31 Chiron Corporation Gensequenzen aus pichia pastoris und deren verwendung
RU2150501C1 (ru) * 1999-02-17 2000-06-10 Эльдаров Михаил Анатольевич Штамм дрожжей saccharomyces cerevisiae-bkm cr-349d-продуцент эпидермального фактора роста человека
US7351549B2 (en) * 2000-01-24 2008-04-01 Polymun Scientific Immunbiologische Forschung Gmbh Method for the manufacture of recombinant trypsin
JP2003530838A (ja) * 2000-04-12 2003-10-21 ヒューマン ゲノム サイエンシズ インコーポレイテッド アルブミン融合タンパク質
US20050100991A1 (en) * 2001-04-12 2005-05-12 Human Genome Sciences, Inc. Albumin fusion proteins
AU2002210126A1 (en) 2000-05-25 2001-12-03 Chiron Corporation Iron regulation of the ppsec10 transcriptional regulatory region of pichia pastoris and methods of use
US7507413B2 (en) * 2001-04-12 2009-03-24 Human Genome Sciences, Inc. Albumin fusion proteins
US20050054051A1 (en) * 2001-04-12 2005-03-10 Human Genome Sciences, Inc. Albumin fusion proteins
ES2425738T3 (es) * 2001-12-21 2013-10-17 Human Genome Sciences, Inc. Proteínas de fusión de la albúmina
AU2002364587A1 (en) * 2001-12-21 2003-07-30 Human Genome Sciences, Inc. Albumin fusion proteins
EP1594530A4 (de) 2003-01-22 2006-10-11 Human Genome Sciences Inc Albuminfusionsproteine
EP2225375A1 (de) * 2007-12-21 2010-09-08 Fujifilm Manufacturing Europe B.V. Verbesserung des ausscheidungsertrags eines zielproteins mittels proteolytischer in-vivo-verarbeitung eines multimeren vorläufers
WO2011011733A2 (en) * 2009-07-24 2011-01-27 The Regents Of The University Of Michigan Factor replacement therapy
US10364421B2 (en) 2015-02-06 2019-07-30 Cargill, Incorporated Modified glucoamylase enzymes and yeast strains having enhanced bioproduct production
CA2979410A1 (en) 2015-03-27 2016-10-06 Cargill, Incorporated Glucoamylase-modified yeast strains and methods for bioproduct production
EP3390637A4 (de) 2015-12-17 2019-05-01 Cargill, Incorporated Zuckertransporter-modifizierte hefestämme und verfahren zur herstellung von bioprodukten
CN109415735B (zh) * 2016-05-04 2020-11-13 深圳普罗吉医药科技有限公司 高效表达重组人血清白蛋白工程菌的构建
BR112019002238A2 (pt) 2016-08-05 2019-05-14 Cargill, Incorporated polipeptídeo e célula manipulados, e, método de fermentação.
KR102794718B1 (ko) 2022-05-16 2025-04-14 전남대학교산학협력단 하이브리드 신호서열을 포함하는 재조합 벡터 및 이를 이용한 인간 인슐린 유사 성장인자-1의 분비 생산방법

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4546082A (en) * 1982-06-17 1985-10-08 Regents Of The Univ. Of California E. coli/Saccharomyces cerevisiae plasmid cloning vector containing the alpha-factor gene for secretion and processing of hybrid proteins
DE3486216T2 (de) * 1983-04-25 1994-02-17 Chiron Corp Hybrid-DNS-Synthesis von reifen insulinähnlichen Wachstumsfaktoren.
US4870008A (en) * 1983-08-12 1989-09-26 Chiron Corporation Secretory expression in eukaryotes
WO1986000637A1 (en) * 1984-07-09 1986-01-30 Integrated Genetics, Inc. Yeast cloning vehicle
US5070075A (en) * 1986-01-07 1991-12-03 Washington University Human preproinsulin-like growth factor I
US4963665A (en) * 1986-01-07 1990-10-16 Washington University Human preproinsulin-like growth factor I
KR100195632B1 (ko) * 1988-07-23 1999-06-15 스티븐 조지 가랜드 펩티드 및 dna 배열
DK105489D0 (da) * 1989-03-03 1989-03-03 Novo Nordisk As Polypeptid
CA2090969C (en) * 1990-09-04 2001-08-21 Russell Arthur Brierley Production of insulin-like growth factor-1 in methylotrophic yeast cells
US5268273A (en) * 1990-12-14 1993-12-07 Phillips Petroleum Company Pichia pastoris acid phosphatase gene, gene regions, signal sequence and expression vectors comprising same

Also Published As

Publication number Publication date
FI961238A0 (fi) 1996-03-15
EP0728191A1 (de) 1996-08-28
AU7604794A (en) 1995-04-03
EP0728191A4 (de) 1999-07-14
DE69432185T2 (de) 2004-02-05
DE69432185D1 (de) 2003-04-03
FI961238L (fi) 1996-05-10
JPH09502609A (ja) 1997-03-18
FI961238A7 (fi) 1996-05-10
AU689496B2 (en) 1998-04-02
WO1995007978A1 (en) 1995-03-23
NZ271928A (en) 1997-10-24
EP0728191B1 (de) 2003-02-26
US5521086A (en) 1996-05-28

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