ATE233314T1 - Eine sekretionssequenz für die herstellung von heterologen proteinen in hefe - Google Patents

Eine sekretionssequenz für die herstellung von heterologen proteinen in hefe

Info

Publication number
ATE233314T1
ATE233314T1 AT94926033T AT94926033T ATE233314T1 AT E233314 T1 ATE233314 T1 AT E233314T1 AT 94926033 T AT94926033 T AT 94926033T AT 94926033 T AT94926033 T AT 94926033T AT E233314 T1 ATE233314 T1 AT E233314T1
Authority
AT
Austria
Prior art keywords
yeast
sequence encoding
dna molecule
protein
heterologue
Prior art date
Application number
AT94926033T
Other languages
English (en)
Inventor
Richard W Scott
Russell Arthur Brierley
David Scott Howland
Original Assignee
Cephalon Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cephalon Inc filed Critical Cephalon Inc
Application granted granted Critical
Publication of ATE233314T1 publication Critical patent/ATE233314T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
AT94926033T 1993-09-16 1994-08-24 Eine sekretionssequenz für die herstellung von heterologen proteinen in hefe ATE233314T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/122,889 US5521086A (en) 1993-09-16 1993-09-16 Secretion sequence for the production of a heterologous protein in yeast
PCT/US1994/009653 WO1995007978A1 (en) 1993-09-16 1994-08-24 A secretion sequence for the production of a heterologous protein in yeast

Publications (1)

Publication Number Publication Date
ATE233314T1 true ATE233314T1 (de) 2003-03-15

Family

ID=22405437

Family Applications (1)

Application Number Title Priority Date Filing Date
AT94926033T ATE233314T1 (de) 1993-09-16 1994-08-24 Eine sekretionssequenz für die herstellung von heterologen proteinen in hefe

Country Status (9)

Country Link
US (1) US5521086A (de)
EP (1) EP0728191B1 (de)
JP (1) JPH09502609A (de)
AT (1) ATE233314T1 (de)
AU (1) AU689496B2 (de)
DE (1) DE69432185T2 (de)
FI (1) FI961238A7 (de)
NZ (1) NZ271928A (de)
WO (1) WO1995007978A1 (de)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE95236T1 (de) * 1983-04-25 1993-10-15 Chiron Corp Hybrid-dns-synthesis von reifen insulinaehnlichen wachstumsfaktoren.
FR2686899B1 (fr) * 1992-01-31 1995-09-01 Rhone Poulenc Rorer Sa Nouveaux polypeptides biologiquement actifs, leur preparation et compositions pharmaceutiques les contenant.
WO1996007744A1 (en) * 1994-09-08 1996-03-14 Chiron Corporation A method of improved production of insulin-like growth factor
JP2001512684A (ja) 1997-08-05 2001-08-28 カイロン コーポレイション 新規のｐｉｃｈｉａｐａｓｔｏｒｉｓ遺伝子配列およびそれらの使用方法
RU2150501C1 (ru) * 1999-02-17 2000-06-10 Эльдаров Михаил Анатольевич Штамм дрожжей saccharomyces cerevisiae-bkm cr-349d-продуцент эпидермального фактора роста человека
US7351549B2 (en) * 2000-01-24 2008-04-01 Polymun Scientific Immunbiologische Forschung Gmbh Method for the manufacture of recombinant trypsin
US20050100991A1 (en) * 2001-04-12 2005-05-12 Human Genome Sciences, Inc. Albumin fusion proteins
US6926898B2 (en) * 2000-04-12 2005-08-09 Human Genome Sciences, Inc. Albumin fusion proteins
AU2002210126A1 (en) 2000-05-25 2001-12-03 Chiron Corporation Iron regulation of the ppsec10 transcriptional regulatory region of pichia pastoris and methods of use
US20050054051A1 (en) * 2001-04-12 2005-03-10 Human Genome Sciences, Inc. Albumin fusion proteins
US7507413B2 (en) * 2001-04-12 2009-03-24 Human Genome Sciences, Inc. Albumin fusion proteins
CA2841097A1 (en) 2001-12-21 2003-07-24 Human Genome Sciences, Inc. Albumin and g-csf fusion proteins
WO2005003296A2 (en) 2003-01-22 2005-01-13 Human Genome Sciences, Inc. Albumin fusion proteins
WO2003059934A2 (en) 2001-12-21 2003-07-24 Human Genome Sciences, Inc. Albumin fusion proteins
JP2011507507A (ja) * 2007-12-21 2011-03-10 フジフィルム・マニュファクチュアリング・ヨーロッパ・ベスローテン・フエンノートシャップ マルチマー性前駆体のｉｎｖｉｖｏタンパク質分解的プロセシングによる関心対象のタンパク質の分泌収量の改善
WO2011011733A2 (en) * 2009-07-24 2011-01-27 The Regents Of The University Of Michigan Factor replacement therapy
EP3253779A1 (de) 2015-02-06 2017-12-13 Cargill, Incorporated Modifizierte glucoamylaseenzyme und hefestämme mit verbesserter bioproduktherstellung
EP3274461A1 (de) 2015-03-27 2018-01-31 Cargill, Incorporated Glucoamylase-modifizierte hefestämme und verfahren zur bioproduktherstellung
BR112018011902A2 (pt) 2015-12-17 2018-12-04 Cargill Inc método de fermentação, levedura geneticamente modificada, construto de ácido nucleico, vetor, célula hospedeira, meio de fermentação e utilização da levedura geneticamente modificada
JP7014774B2 (ja) * 2016-05-04 2022-02-01 シェンチェン、プロトゲン、リミテッド 組換えヒト血清アルブミンの高発現のための遺伝子工学操作された細菌の構築
WO2018027131A1 (en) 2016-08-05 2018-02-08 Cargill, Incorporated Leader-modified glucoamylase polypeptides and engineered yeast strains having enhanced bioproduct production
KR102794718B1 (ko) 2022-05-16 2025-04-14 전남대학교산학협력단 하이브리드 신호서열을 포함하는 재조합 벡터 및 이를 이용한 인간 인슐린 유사 성장인자-1의 분비 생산방법

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4546082A (en) * 1982-06-17 1985-10-08 Regents Of The Univ. Of California E. coli/Saccharomyces cerevisiae plasmid cloning vector containing the alpha-factor gene for secretion and processing of hybrid proteins
ATE95236T1 (de) * 1983-04-25 1993-10-15 Chiron Corp Hybrid-dns-synthesis von reifen insulinaehnlichen wachstumsfaktoren.
US4870008A (en) * 1983-08-12 1989-09-26 Chiron Corporation Secretory expression in eukaryotes
JPS61502655A (ja) * 1984-07-09 1986-11-20 インテグレ−テッド・ジェネティックス・インコ−ポレ−テッド 酵母クロ−ニングビヒクル
US5070075A (en) * 1986-01-07 1991-12-03 Washington University Human preproinsulin-like growth factor I
US4963665A (en) * 1986-01-07 1990-10-16 Washington University Human preproinsulin-like growth factor I
ATE135045T1 (de) * 1988-07-23 1996-03-15 Delta Biotechnology Ltd Sekretorische leader-sequenzen
DK105489D0 (da) * 1989-03-03 1989-03-03 Novo Nordisk As Polypeptid
CA2090969C (en) * 1990-09-04 2001-08-21 Russell Arthur Brierley Production of insulin-like growth factor-1 in methylotrophic yeast cells
US5268273A (en) * 1990-12-14 1993-12-07 Phillips Petroleum Company Pichia pastoris acid phosphatase gene, gene regions, signal sequence and expression vectors comprising same

Also Published As

Publication number Publication date
NZ271928A (en) 1997-10-24
EP0728191A1 (de) 1996-08-28
FI961238L (fi) 1996-05-10
DE69432185D1 (de) 2003-04-03
EP0728191B1 (de) 2003-02-26
FI961238A0 (fi) 1996-03-15
AU689496B2 (en) 1998-04-02
DE69432185T2 (de) 2004-02-05
JPH09502609A (ja) 1997-03-18
WO1995007978A1 (en) 1995-03-23
US5521086A (en) 1996-05-28
EP0728191A4 (de) 1999-07-14
AU7604794A (en) 1995-04-03
FI961238A7 (fi) 1996-05-10

Similar Documents

Publication Publication Date Title
DE69432185D1 (de) Eine sekretionssequenz für die herstellung von heterologen proteinen in hefe
NZ232742A (en) Yeast heterologous protein production method, vectors, dna constructs and proteins so produced and aprotinin (1-58) so produced
ATE121781T1 (de) Verwendung von dns-fragmenten, die ein signalpeptid kodieren zur sekretion von reifen proteinen in rekombinanten hefen, expressions- kassetten, transformierte hefen und verfahren zur herstellung dieser proteine.
AU595262B2 (en) Eukaryotic fusion proteins, the preparation and use thereof and means for carrying out the process
PT755447E (pt) Proteinas recombinantes de hemaglutinina filamentosa de bordetella particularmente bordetella pertussis metodo para a sua producao e suas aplicaoes na producao de proteinas estranhas ou de principios activos para vacinas
WO1997028247A3 (fr) PROTEINES DE RESERVE DE PLANTES ENRICHIES EN ACIDES AMINES, NOTAMMENT η-ZEINE DE MAIS ENRICHIE EN LYSINE, PLANTES EXPRIMANT CES PROTEINES
DE69929805D1 (de) Modifiziertes e.coli enterotoxin ii signalpeptid sowie ein mikroorganismus der ein fusionsprotein sowie ein heterologes protein exprimiert
ATE121454T1 (de) Ubiquitinspezifische protease.
IL76107A0 (en) Method of removing n-terminal amino acid residues from eucaryotic polypeptide analogs and polypeptides produced thereby
IL85020A (en) Pancreatic secretory trypsin inhibitor variants, their production, expression vectors and recombinant hosts therefor and pharmaceutical compositions containing them
WO2003083043A3 (en) Methods for producing secreted polypeptides having l-asparaginase activity
KR970065558A (ko) 분비형 Kex2 유도체의 제조방법
BG101226A (bg) Метод за производство на модифициран инхибитор палидипин на колагениндуцирано тромбоцитно натрупване
ATE154362T1 (de) Verfahren zur herstellung von pai-2
IL99079A0 (en) Protein structure of the plant toxin gelonin
NO911557L (no) Fremgangsmaate for fremstilling av fremmedproteiner i streptomyceter.
CA2198966A1 (en) Method for cleaving chimeric protein using processing enzyme
CA2171557A1 (en) A secretion sequence for the production of a heterologous protein in yeast
ATE141645T1 (de) In vitro-behandlung von fusionsproteinen
ATE224403T1 (de) Verwendung von kaseinfragmenten als wachstumerförderer
JPS642583A (en) Recombinant dna and production of polypeptide using said dna
Zagnitko et al. Thiol-dependent serine proteinases. III. Amino acid sequences adjacent to active site serine residue in Bacillus thuringiensis and Bacillus cereus proteinases.
Bjorn et al. Yeast processing system
HUT67346A (en) Recombinant viruses comprising artificial proteolytic cleavage site

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties