ATE233314T1 - Eine sekretionssequenz für die herstellung von heterologen proteinen in hefe - Google Patents

Eine sekretionssequenz für die herstellung von heterologen proteinen in hefe

Info

Publication number
ATE233314T1
ATE233314T1 AT94926033T AT94926033T ATE233314T1 AT E233314 T1 ATE233314 T1 AT E233314T1 AT 94926033 T AT94926033 T AT 94926033T AT 94926033 T AT94926033 T AT 94926033T AT E233314 T1 ATE233314 T1 AT E233314T1
Authority
AT
Austria
Prior art keywords
yeast
sequence encoding
dna molecule
protein
heterologue
Prior art date
Application number
AT94926033T
Other languages
English (en)
Inventor
Richard W Scott
Russell Arthur Brierley
David Scott Howland
Original Assignee
Cephalon Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cephalon Inc filed Critical Cephalon Inc
Application granted granted Critical
Publication of ATE233314T1 publication Critical patent/ATE233314T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/65—Insulin-like growth factors, i.e. somatomedins, e.g. IGF-1, IGF-2
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
AT94926033T 1993-09-16 1994-08-24 Eine sekretionssequenz für die herstellung von heterologen proteinen in hefe ATE233314T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/122,889 US5521086A (en) 1993-09-16 1993-09-16 Secretion sequence for the production of a heterologous protein in yeast
PCT/US1994/009653 WO1995007978A1 (en) 1993-09-16 1994-08-24 A secretion sequence for the production of a heterologous protein in yeast

Publications (1)

Publication Number Publication Date
ATE233314T1 true ATE233314T1 (de) 2003-03-15

Family

ID=22405437

Family Applications (1)

Application Number Title Priority Date Filing Date
AT94926033T ATE233314T1 (de) 1993-09-16 1994-08-24 Eine sekretionssequenz für die herstellung von heterologen proteinen in hefe

Country Status (9)

Country Link
US (1) US5521086A (de)
EP (1) EP0728191B1 (de)
JP (1) JPH09502609A (de)
AT (1) ATE233314T1 (de)
AU (1) AU689496B2 (de)
DE (1) DE69432185T2 (de)
FI (1) FI961238A7 (de)
NZ (1) NZ271928A (de)
WO (1) WO1995007978A1 (de)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ207842A (en) * 1983-04-25 1988-02-12 Chiron Corp Production of human insulin-like growth factor (igf) using cdna
FR2686899B1 (fr) * 1992-01-31 1995-09-01 Rhone Poulenc Rorer Sa Nouveaux polypeptides biologiquement actifs, leur preparation et compositions pharmaceutiques les contenant.
EP0779927A1 (de) * 1994-09-08 1997-06-25 Chiron Corporation Methode zur gesteigerten herstellung von insulin-ähnlichem wachstumsfaktor
JP2001512684A (ja) 1997-08-05 2001-08-28 カイロン コーポレイション 新規のｐｉｃｈｉａｐａｓｔｏｒｉｓ遺伝子配列およびそれらの使用方法
RU2150501C1 (ru) * 1999-02-17 2000-06-10 Эльдаров Михаил Анатольевич Штамм дрожжей saccharomyces cerevisiae-bkm cr-349d-продуцент эпидермального фактора роста человека
US7351549B2 (en) * 2000-01-24 2008-04-01 Polymun Scientific Immunbiologische Forschung Gmbh Method for the manufacture of recombinant trypsin
EP2213743A1 (de) 2000-04-12 2010-08-04 Human Genome Sciences, Inc. Albuminfusionsproteine
US20050100991A1 (en) * 2001-04-12 2005-05-12 Human Genome Sciences, Inc. Albumin fusion proteins
US6605450B1 (en) 2000-05-25 2003-08-12 Chiron Corporation Iron regulation of the PpSEC10 transcriptional regulatory region of Pichia pastoris and methods of use
US7507413B2 (en) * 2001-04-12 2009-03-24 Human Genome Sciences, Inc. Albumin fusion proteins
US20050054051A1 (en) * 2001-04-12 2005-03-10 Human Genome Sciences, Inc. Albumin fusion proteins
WO2003059934A2 (en) 2001-12-21 2003-07-24 Human Genome Sciences, Inc. Albumin fusion proteins
AU2002364586A1 (en) * 2001-12-21 2003-07-30 Delta Biotechnology Limited Albumin fusion proteins
WO2005003296A2 (en) 2003-01-22 2005-01-13 Human Genome Sciences, Inc. Albumin fusion proteins
WO2009082209A1 (en) * 2007-12-21 2009-07-02 Fujifilm Manufacturing Europe B.V. Improvement of the secretion yield of a protein of interest by in vivo proteolytic processing of a multimeric precursor
US9023798B2 (en) 2009-07-24 2015-05-05 The Regents Of The University Of Michigan Cystinosin replacement factor
CN107250163A (zh) 2015-02-06 2017-10-13 嘉吉公司 修饰的葡糖淀粉酶和具有增强的生物产物产生的酵母菌株
EP3274461A1 (de) 2015-03-27 2018-01-31 Cargill, Incorporated Glucoamylase-modifizierte hefestämme und verfahren zur bioproduktherstellung
WO2017106739A1 (en) 2015-12-17 2017-06-22 Cargill, Incorporated Sugar transporter-modified yeast strains and methods for bioproduct production
WO2017190671A1 (zh) * 2016-05-04 2017-11-09 北京普罗吉生物科技发展有限公司 高效表达重组人血清白蛋白工程菌的构建
WO2018027131A1 (en) 2016-08-05 2018-02-08 Cargill, Incorporated Leader-modified glucoamylase polypeptides and engineered yeast strains having enhanced bioproduct production
KR102794718B1 (ko) 2022-05-16 2025-04-14 전남대학교산학협력단 하이브리드 신호서열을 포함하는 재조합 벡터 및 이를 이용한 인간 인슐린 유사 성장인자-1의 분비 생산방법

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4546082A (en) * 1982-06-17 1985-10-08 Regents Of The Univ. Of California E. coli/Saccharomyces cerevisiae plasmid cloning vector containing the alpha-factor gene for secretion and processing of hybrid proteins
NZ207842A (en) * 1983-04-25 1988-02-12 Chiron Corp Production of human insulin-like growth factor (igf) using cdna
US4870008A (en) * 1983-08-12 1989-09-26 Chiron Corporation Secretory expression in eukaryotes
EP0188555A4 (de) * 1984-07-09 1988-04-18 Integrated Genetics Inc Klonierungsvektor für hefe.
US4963665A (en) * 1986-01-07 1990-10-16 Washington University Human preproinsulin-like growth factor I
US5070075A (en) * 1986-01-07 1991-12-03 Washington University Human preproinsulin-like growth factor I
EP0387319B1 (de) * 1988-07-23 1996-03-06 Delta Biotechnology Limited Sekretorische leader-sequenzen
DK105489D0 (da) * 1989-03-03 1989-03-03 Novo Nordisk As Polypeptid
EP0548267A4 (en) * 1990-09-04 1994-11-23 Salk Inst Biotech Ind Production of insulin-like growth factor-1 in methylotrophic yeast cells
US5268273A (en) * 1990-12-14 1993-12-07 Phillips Petroleum Company Pichia pastoris acid phosphatase gene, gene regions, signal sequence and expression vectors comprising same

Also Published As

Publication number Publication date
NZ271928A (en) 1997-10-24
EP0728191A1 (de) 1996-08-28
FI961238A0 (fi) 1996-03-15
DE69432185T2 (de) 2004-02-05
AU7604794A (en) 1995-04-03
DE69432185D1 (de) 2003-04-03
WO1995007978A1 (en) 1995-03-23
FI961238L (fi) 1996-05-10
AU689496B2 (en) 1998-04-02
EP0728191B1 (de) 2003-02-26
US5521086A (en) 1996-05-28
JPH09502609A (ja) 1997-03-18
EP0728191A4 (de) 1999-07-14
FI961238A7 (fi) 1996-05-10

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