ATE236259T1 - Herstellung einzelner genprodukte eines chimären fusionsproteins durch spaltung mit überall vorkommenden endoproteasen - Google Patents

Herstellung einzelner genprodukte eines chimären fusionsproteins durch spaltung mit überall vorkommenden endoproteasen

Info

Publication number: ATE236259T1
Authority: AT; Austria
Prior art keywords: expression; gene products; fusion protein; sequence encoding; endoproteases
Prior art date: 1998-05-21

Application number

AT99923745T

Other languages

English (en)

Inventor

Johannes Adrianus Gaeken

Farzin Farzaneh

Stephen James Russell

Original Assignee

Cancer Rec Tech Ltd

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1998-05-21

Filing date

1999-05-21

Publication date

2003-04-15

1999-05-21 Application filed by Cancer Rec Tech Ltd filed Critical Cancer Rec Tech Ltd

2003-04-15 Application granted granted Critical

2003-04-15 Publication of ATE236259T1 publication Critical patent/ATE236259T1/de

Links

108090000623 proteins and genes Proteins 0.000 title abstract 5
108020001507 fusion proteins Proteins 0.000 title abstract 2
229920001184 polypeptide Polymers 0.000 abstract 3
102000004196 processed proteins & peptides Human genes 0.000 abstract 3
108090000765 processed proteins & peptides Proteins 0.000 abstract 3
102000002067 Protein Subunits Human genes 0.000 abstract 2
108010001267 Protein Subunits Proteins 0.000 abstract 2
102000004169 proteins and genes Human genes 0.000 abstract 2
101710118538 Protease Proteins 0.000 abstract 1
238000003776 cleavage reaction Methods 0.000 abstract 1
239000000470 constituent Substances 0.000 abstract 1
102000037865 fusion proteins Human genes 0.000 abstract 1
239000000463 material Substances 0.000 abstract 1
238000000034 method Methods 0.000 abstract 1
102000039446 nucleic acids Human genes 0.000 abstract 1
108020004707 nucleic acids Proteins 0.000 abstract 1
150000007523 nucleic acids Chemical class 0.000 abstract 1
230000007017 scission Effects 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/54—Interleukins [IL]
- C07K14/55—IL-2
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4721—Lipocortins
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/54—Interleukins [IL]
- C07K14/5406—IL-4
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/54—Interleukins [IL]
- C07K14/5425—IL-9
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/54—Interleukins [IL]
- C07K14/5434—IL-12
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/515—Animal cells
- A61K2039/5152—Tumor cells
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide

Landscapes

Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Organic Chemistry (AREA)
Genetics & Genomics (AREA)
Molecular Biology (AREA)
Zoology (AREA)
General Health & Medical Sciences (AREA)
Biophysics (AREA)
Biochemistry (AREA)
Medicinal Chemistry (AREA)
Gastroenterology & Hepatology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Toxicology (AREA)
Engineering & Computer Science (AREA)
Bioinformatics & Cheminformatics (AREA)
Biomedical Technology (AREA)
Immunology (AREA)
Wood Science & Technology (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Public Health (AREA)
Animal Behavior & Ethology (AREA)
Chemical Kinetics & Catalysis (AREA)
Pharmacology & Pharmacy (AREA)
Physics & Mathematics (AREA)
Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
General Chemical & Material Sciences (AREA)
Plant Pathology (AREA)
Microbiology (AREA)
Veterinary Medicine (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Peptides Or Proteins (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Saccharide Compounds (AREA)
Sorting Of Articles (AREA)

AT99923745T 1998-05-21 1999-05-21 Herstellung einzelner genprodukte eines chimären fusionsproteins durch spaltung mit überall vorkommenden endoproteasen ATE236259T1 (de)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
GBGB9810999.4A GB9810999D0 (en)	1998-05-21	1998-05-21	Materials and methods relating to the expression of genes
PCT/GB1999/001609 WO1999060135A1 (en)	1998-05-21	1999-05-21	Generation of multiple gene products from chimeric fusion proteins by cleavage with ubiquitous endoproteases

Publications (1)

Publication Number	Publication Date
ATE236259T1 true ATE236259T1 (de)	2003-04-15

Family

ID=10832516

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AT99923745T ATE236259T1 (de)	1998-05-21	1999-05-21	Herstellung einzelner genprodukte eines chimären fusionsproteins durch spaltung mit überall vorkommenden endoproteasen

Country Status (13)

Country	Link
EP (1)	EP1080206B1 (de)
JP (1)	JP2002515251A (de)
KR (1)	KR20010052383A (de)
CN (1)	CN1308679A (de)
AT (1)	ATE236259T1 (de)
AU (1)	AU753785B2 (de)
CA (1)	CA2333144A1 (de)
DE (1)	DE69906509D1 (de)
ES (1)	ES2201718T3 (de)
GB (1)	GB9810999D0 (de)
ID (1)	ID27206A (de)
NZ (1)	NZ508863A (de)
WO (1)	WO1999060135A1 (de)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US6586411B1 (en)	2000-08-16	2003-07-01	Mayo Foundation For Medical Education And Research	System for monitoring the location of transgenes
US7169603B2 (en)	2000-07-14	2007-01-30	Mgi Pharma Biologics, Inc.	α-MSH related compounds and methods of use
EP1369128A1 (de) *	2002-06-07	2003-12-10	Procorde GmbH	Glycoprotein VI Inhibitoren und deren therapeutische Verwendung
ATE473239T1 (de) *	2003-05-14	2010-07-15	Bayer Pharmaceuticals Corp	Interleukin-9 antagonist-muteine und ihre pharmakologischen anwendungsverfahren
JP4644663B2 (ja) *	2003-06-03	2011-03-02	セルジェネシスインコーポレイテッド	ペプチド開裂部位を用いた単一ベクターからの組換えポリペプチドの高められた発現のための構成と方法
US7485291B2 (en) *	2003-06-03	2009-02-03	Cell Genesys, Inc.	Compositions and methods for generating multiple polypeptides from a single vector using a virus derived peptide cleavage site, and uses thereof
FR2856069A1 (fr) *	2003-06-10	2004-12-17	Bionexis	Molecules de ciblage et de liberation de composes therapeutiques et leur utilisation
BRPI0613784A2 (pt)	2005-07-21	2011-02-01	Abbott Lab	expressão de gene múltipla incluindo constructos sorf e métodos com poliproteìnas, pro-proteìnas e proteólise
CA2698357C (en) *	2007-09-04	2017-06-06	The Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services	Deletions in domain ii of pseudomonas exotoxin a that reduce non-specific toxicity
US8715964B2 (en)	2008-05-11	2014-05-06	The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services	Expression of IL-12 family heterodimers
WO2011042874A1 (en) *	2009-10-06	2011-04-14	Ecole Polytechnique Federale De Lausanne (Epfl)	Visualization of proprotein convertase activity in living cells and tissues
US9951117B2 (en)	2010-09-02	2018-04-24	Mayo Foundation For Medical Education And Research	Vesicular stomatitis viruses
KR101911964B1 (ko)	2010-09-02	2018-10-25	메이오 파운데이션 포 메디칼 에쥬케이션 앤드 리써치	수포성 구내염 바이러스
US20170291934A1 (en) *	2014-09-22	2017-10-12	Charles C. Reed	Improved therapeutic control of heterodimeric and single chain forms of interleukin-12
WO2017062953A1 (en) *	2015-10-10	2017-04-13	Intrexon Corporation	Improved therapeutic control of proteolytically sensitive, destabilized forms of interleukin-12
CA3086486A1 (en)	2018-09-17	2020-03-26	Gi Innovation, Inc.	Fusion protein comprising il-2 protein and cd80 protein, and use thereof
EP3941531A4 (de) *	2019-03-22	2023-01-18	The Johns Hopkins University	Genfreisetzungspartikel zur induzierung von antigen-präsentierenden zellen aus tumoren
CA3213789A1 (en)	2021-04-02	2022-10-06	Krystal Biotech, Inc.	Viral vectors for cancer therapy
JP2024540002A (ja) *	2021-10-25	2024-10-31	ジェンビーボ・インコーポレイテッド	治療剤送達のための組成物および方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
AU6104586A (en) *	1985-08-12	1987-02-19	Malcolm A. Martin	Aids protein using trple gene
DK0485504T3 (da) *	1989-08-11	1994-04-18	Zymogenetics Inc	Fremgangsmåde til fremstilling af aktiveret protein C ved celledyrkning
JPH11513249A (ja) *	1995-09-27	1999-11-16	メディカル・リサーチ・カウンシル	プロテアーゼにより開裂可能なタンパク質を組込んだ組換えウイルス

1998
- 1998-05-21 GB GBGB9810999.4A patent/GB9810999D0/en not_active Ceased
1999
- 1999-05-21 AU AU40508/99A patent/AU753785B2/en not_active Ceased
- 1999-05-21 KR KR1020007013081A patent/KR20010052383A/ko not_active Withdrawn
- 1999-05-21 ID IDW20002666A patent/ID27206A/id unknown
- 1999-05-21 AT AT99923745T patent/ATE236259T1/de not_active IP Right Cessation
- 1999-05-21 WO PCT/GB1999/001609 patent/WO1999060135A1/en not_active Ceased
- 1999-05-21 CA CA002333144A patent/CA2333144A1/en not_active Abandoned
- 1999-05-21 DE DE69906509T patent/DE69906509D1/de not_active Expired - Lifetime
- 1999-05-21 NZ NZ508863A patent/NZ508863A/xx unknown
- 1999-05-21 EP EP99923745A patent/EP1080206B1/de not_active Expired - Lifetime
- 1999-05-21 CN CN99808292A patent/CN1308679A/zh active Pending
- 1999-05-21 JP JP2000549741A patent/JP2002515251A/ja active Pending
- 1999-05-21 ES ES99923745T patent/ES2201718T3/es not_active Expired - Lifetime

Also Published As

Publication number	Publication date
JP2002515251A (ja)	2002-05-28
GB9810999D0 (en)	1998-07-22
CA2333144A1 (en)	1999-11-25
WO1999060135A1 (en)	1999-11-25
WO1999060135A8 (en)	2000-01-13
DE69906509D1 (de)	2003-05-08
KR20010052383A (ko)	2001-06-25
NZ508863A (en)	2003-03-28
EP1080206B1 (de)	2003-04-02
ES2201718T3 (es)	2004-03-16
CN1308679A (zh)	2001-08-15
AU753785B2 (en)	2002-10-31
ID27206A (id)	2001-03-08
EP1080206A1 (de)	2001-03-07
AU4050899A (en)	1999-12-06

Legal Events

Date	Code	Title	Description
2003-10-15	RER	Ceased as to paragraph 5 lit. 3 law introducing patent treaties

Publication	Publication Date	Title
ATE236259T1 (de)	2003-04-15	Herstellung einzelner genprodukte eines chimären fusionsproteins durch spaltung mit überall vorkommenden endoproteasen
CN101253196B (zh)	2012-09-05	通过胰蛋白酶变体切割胰岛素前体
WO1994018332A3 (en)	1994-10-13	Anthrax toxin fusion proteins and uses thereof
DK0941318T3 (da)	2001-05-21	Håndvægtformede konstruerede ekspressionssystemer til genterapi
CA2472477A1 (en)	2003-07-17	Methods and compositions for protein expression and purification
ATE264393T1 (de)	2004-04-15	Klonierung der prolyl-dipeptidyl-peptidase von aspergillus oryzae
HU212592B (en)	1996-08-29	Method for producing antibodies genetechnologically
WO2001034824A2 (en)	2001-05-17	Methods and compositions comprising renilla gfp
ATE542913T1 (de)	2012-02-15	Promotorvarianten zur expression von genen in einer pilzzelle
MXPA05012336A (es)	2006-01-30	Procedimiento para producir una proteina dirigida a un plastidio en celulas vegetales.
EP0127658B1 (de)	1987-10-14	Verfahren zur herstellung reifer proteine aus schmelzproteinen hergestellt in pro- oder eukaryotischen zellen
WO2003016477A3 (en)	2006-11-30	Insect or83b odorant receptor
EP1458240A4 (de)	2006-01-25	Zur glucoseregulierten produktion von menschlichem insulin in somatischen zelllinien geeignete nukleinsäurekonstrukte
EP0955370A3 (de)	2000-12-20	DNAs die für neue fusions-Proteine kodieren und Verfahren zur Herstellung von nützlichen Polypeptiden durch Expression der DNAs
Wong et al.	2018	Escherichia coli: A versatile platform for recombinant protein expression
RU2619217C1 (ru)	2017-05-12	Температурочувствительный мутантный интеин для нерастворимой экспрессии предшественника целевого белка
SE0303093D0 (sv)	2003-11-18	Expression vector, host cell and method for producing fusion proteins
WO2002006485A3 (en)	2003-03-13	47885, a novel human ubiquitin-activating enzyme and uses therefor
ATE354663T1 (de)	2007-03-15	Eine mycobacterium tuberculosis kodierende sequenz zur expression von heterologen proteinen
DE602004020771D1 (de)	2009-06-04	Recombinante igf expressionssysteme
WO2001081584A3 (en)	2002-04-04	27960, a novel ubiquitin conjugating enzyme family member and uses therefor
AU2002365538A1 (en)	2003-06-10	Production of eukaryotic proteins and nucleic acid molecules in c. elegans
AU2002234132A1 (en)	2002-05-06	13245, a novel human myotonic dystrophy type protein kinase and uses therefor
WO2002024898A3 (en)	2003-03-27	47647, a human lipase and uses therefor
IT1186750B (it)	1987-12-16	Vettore di clonaggio,molecole di dna ricombinante,ceppi di bacillus subtilis trasformati con dette molecole e metodi per l'espressione di geni eterologhi e produzione e secrezione di proteine codificate da detti geni