ATE239090T1 - Methode zum generieren von einzelsträngigen dna molekülen - Google Patents

Methode zum generieren von einzelsträngigen dna molekülen

Info

Publication number
ATE239090T1
ATE239090T1 AT94907855T AT94907855T ATE239090T1 AT E239090 T1 ATE239090 T1 AT E239090T1 AT 94907855 T AT94907855 T AT 94907855T AT 94907855 T AT94907855 T AT 94907855T AT E239090 T1 ATE239090 T1 AT E239090T1
Authority
AT
Austria
Prior art keywords
stranded dna
single stranded
dna molecules
generating single
generating
Prior art date
Application number
AT94907855T
Other languages
English (en)
Inventor
Theo Nikiforov
Michael R Knapp
Original Assignee
Orchid Biosciences Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Orchid Biosciences Inc filed Critical Orchid Biosciences Inc
Application granted granted Critical
Publication of ATE239090T1 publication Critical patent/ATE239090T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT94907855T 1993-01-15 1994-01-18 Methode zum generieren von einzelsträngigen dna molekülen ATE239090T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US506193A 1993-01-15 1993-01-15
US08/155,746 US5518900A (en) 1993-01-15 1993-11-23 Method for generating single-stranded DNA molecules
PCT/US1994/000771 WO1994016090A1 (en) 1993-01-15 1994-01-18 Method for generating single-stranded dna molecules

Publications (1)

Publication Number Publication Date
ATE239090T1 true ATE239090T1 (de) 2003-05-15

Family

ID=26673870

Family Applications (1)

Application Number Title Priority Date Filing Date
AT94907855T ATE239090T1 (de) 1993-01-15 1994-01-18 Methode zum generieren von einzelsträngigen dna molekülen

Country Status (8)

Country Link
US (1) US5518900A (de)
EP (1) EP0679190B1 (de)
JP (2) JP3330946B2 (de)
AT (1) ATE239090T1 (de)
AU (1) AU674211B2 (de)
CA (1) CA2153898C (de)
DE (1) DE69432586T2 (de)
WO (1) WO1994016090A1 (de)

Families Citing this family (131)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5817797A (en) * 1988-06-01 1998-10-06 The United States Of America As Represented By The Department Of Health And Human Services Sequencing DNA; a modification of the polymerase chain reaction
US6436635B1 (en) 1992-11-06 2002-08-20 Boston University Solid phase sequencing of double-stranded nucleic acids
US5795714A (en) 1992-11-06 1998-08-18 Trustees Of Boston University Method for replicating an array of nucleic acid probes
CA2176193A1 (en) * 1995-05-22 1996-11-23 John Wesley Backus Methods for polymerase chain reaction preamplification sterilization by exonucleases in the presence of phosphorothioated primers
US6015664A (en) * 1995-11-03 2000-01-18 Mcw Research Foundation Multiplex PCR assay using unequal primer concentrations to detect HPIV 1,2,3 and RSV A,B and influenza virus A, B
AU2069597A (en) * 1996-03-04 1997-09-22 Genetrace Systems, Inc. Methods of screening nucleic acids using mass spectrometry
WO1998012355A1 (en) * 1996-09-19 1998-03-26 Genetrace Systems Methods of preparing nucleic acids for mass spectrometric analysis
US5965363A (en) * 1996-09-19 1999-10-12 Genetrace Systems Inc. Methods of preparing nucleic acids for mass spectrometric analysis
EP1164203B1 (de) 1996-11-06 2007-10-10 Sequenom, Inc. DNA-Diagnostik mittels Massenspektrometrie
EP0963443B1 (de) 1996-12-10 2006-03-08 Sequenom, Inc. Abspaltbare, nicht-flüchtige moleküle zur massenmarkierung
US6046005A (en) * 1997-01-15 2000-04-04 Incyte Pharmaceuticals, Inc. Nucleic acid sequencing with solid phase capturable terminators comprising a cleavable linking group
US5876936A (en) * 1997-01-15 1999-03-02 Incyte Pharmaceuticals, Inc. Nucleic acid sequencing with solid phase capturable terminators
US5958738A (en) * 1997-03-24 1999-09-28 Roche Diagnostics Corporation Procedure for subtractive hybridization and difference analysis
ATE335845T1 (de) * 1997-05-16 2006-09-15 Exact Sciences Corp Elektrophoretische analyse von molekülen mit immobilisierten sonden
US6309829B1 (en) * 1997-05-27 2001-10-30 Pe Corporation (Ny) Length determination of nucleic acid repeat sequences by discontinuous primer extension
US5919626A (en) 1997-06-06 1999-07-06 Orchid Bio Computer, Inc. Attachment of unmodified nucleic acids to silanized solid phase surfaces
US7070921B2 (en) 2000-04-28 2006-07-04 Molecular Devices Corporation Molecular modification assays
US6136568A (en) * 1997-09-15 2000-10-24 Hiatt; Andrew C. De novo polynucleotide synthesis using rolling templates
US7745142B2 (en) 1997-09-15 2010-06-29 Molecular Devices Corporation Molecular modification assays
US7632651B2 (en) 1997-09-15 2009-12-15 Mds Analytical Technologies (Us) Inc. Molecular modification assays
US6982431B2 (en) 1998-08-31 2006-01-03 Molecular Devices Corporation Sample analysis systems
US6297018B1 (en) 1998-04-17 2001-10-02 Ljl Biosystems, Inc. Methods and apparatus for detecting nucleic acid polymorphisms
US6322968B1 (en) 1997-11-21 2001-11-27 Orchid Biosciences, Inc. De novo or “universal” sequencing array
WO1999034014A2 (en) * 1997-12-23 1999-07-08 Roche Diagnostics Gmbh A method for the determination of a nucleic acid
GB9806253D0 (en) * 1998-03-25 1998-05-20 Tepnel Medical Ltd Amplification of nucleic acids
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US6104028A (en) 1998-05-29 2000-08-15 Genetrace Systems Inc. Volatile matrices for matrix-assisted laser desorption/ionization mass spectrometry
US6872521B1 (en) 1998-06-16 2005-03-29 Beckman Coulter, Inc. Polymerase signaling assay
CA2330732A1 (en) * 1998-06-19 1999-12-23 Mosaic Technologies Detection of non-viral organisms with srp rna
US6743605B1 (en) * 1998-06-24 2004-06-01 Enzo Life Sciences, Inc. Linear amplification of specific nucleic acid sequences
US6703228B1 (en) 1998-09-25 2004-03-09 Massachusetts Institute Of Technology Methods and products related to genotyping and DNA analysis
EP1001037A3 (de) * 1998-09-28 2003-10-01 Whitehead Institute For Biomedical Research Vorselektion und Isolierung eines Einzelnukleotidpolymorphismus
US20020048772A1 (en) 2000-02-10 2002-04-25 Dahiyat Bassil I. Protein design automation for protein libraries
US7315786B2 (en) 1998-10-16 2008-01-01 Xencor Protein design automation for protein libraries
US6451525B1 (en) * 1998-12-03 2002-09-17 Pe Corporation (Ny) Parallel sequencing method
WO2000036152A1 (en) 1998-12-14 2000-06-22 Li-Cor, Inc. A system and methods for nucleic acid sequencing of single molecules by polymerase synthesis
US6743578B1 (en) 1998-12-18 2004-06-01 The Regents Of The University Of California Method for the detection of specific nucleic acid sequences by polymerase nucleotide incorporation
CA2354682A1 (en) * 1998-12-18 2000-06-29 The Regents Of The University Of California Method for the detection of specific nucleic acid sequences by polymerase nucleotide incorporation
ATE265040T1 (de) * 1999-02-26 2004-05-15 Exact Sciences Corp Biochemische reinigungsgeräte mit immobilisierten fangsonden und ihre anwendungen
US6573047B1 (en) 1999-04-13 2003-06-03 Dna Sciences, Inc. Detection of nucleotide sequence variation through fluorescence resonance energy transfer label generation
WO2000070093A1 (en) * 1999-05-13 2000-11-23 Oligos Etc. Inc. Arrays with modified oligonucleotide and polynucleotide compositions
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US6642000B1 (en) 1999-11-12 2003-11-04 University Of Chicago PCR amplification on microarrays of gel immobilized oligonucleotides
IL149676A0 (en) * 1999-12-02 2002-11-10 Dna Sciences Inc Methods and kits for determining nucleotide variations
US6762018B1 (en) 1999-12-23 2004-07-13 Tetragen Sa Analysis of nucleotide polymorphisms at a site
WO2001049882A2 (en) * 1999-12-29 2001-07-12 Keygene N.V. METHOD FOR GENERATING OLIGONUCLEOTIDES, IN PARTICULAR FOR THE DETECTION OF AMPLIFIED RESTRICTION FRAGMENTS OBTAINED USING AFLP$m(3)
WO2001079225A2 (en) * 2000-04-12 2001-10-25 Genaissance Pharmaceuticals, Inc. Haplotypes of the csf1r gene
CA2302827A1 (en) * 2000-04-12 2001-10-12 Michelle Furtado Oligonucleotide and dna de-hybridization on surfaces
US6355433B1 (en) 2000-06-02 2002-03-12 Dna Sciences, Inc. Determination of nucleotide sequence variations through limited primer extension
DE10029914A1 (de) * 2000-06-19 2002-01-03 Epigenomics Ag Verfahren zur hochparallelen Analyse von Polymorphismen
AU2001273559A1 (en) * 2000-07-18 2002-01-30 Enchira Biotechnology Corporation Methods of ligation mediated chimeragenesis utilizing populations of scaffold and donor nucleic acids
DE10046184A1 (de) * 2000-09-18 2002-04-04 November Ag Molekulare Medizin Verfahren zum Nachweis mindestens einer Nukleinsäuresequenz
DE10104938B4 (de) * 2001-01-29 2005-06-23 Epigenomics Ag Fluoreszenzpolarisation 1
JP2004523243A (ja) 2001-03-12 2004-08-05 カリフォルニア インスティチュート オブ テクノロジー 非同期性塩基伸長によってポリヌクレオチド配列を分析するための方法および装置
DE10119203B4 (de) * 2001-04-19 2004-09-16 Rolf Dr. Postina Verfahren zur Erzeugung von doppelsträngigen Nukleinsäuren mit einzelsträngigen Überhängen beliebiger Länge und Nukleotidzusammensetzung
AU2002303827A1 (en) * 2001-05-25 2002-12-09 Invitrogen Corporation Compositions and methods for extension of nucleic acids
EP1503321A3 (de) * 2001-08-10 2006-08-30 Xencor, Inc. Automatisierte Proteinkonstruktion für Proteinbibliotheken
US7560254B2 (en) * 2001-08-29 2009-07-14 Ge Healthcare Bio-Sciences Corp. Allele specific primer extension
US20050147984A1 (en) * 2001-08-31 2005-07-07 Clondiag Chip Technologies Gmbh Interaction detection on several probe arrays
US6902914B2 (en) * 2001-09-28 2005-06-07 Sigma-Aldrich, Co. Recombinant DNA processes using a dNTP mixture containing modified nucleotides
US7125982B1 (en) * 2001-12-05 2006-10-24 Frayne Consultants Microbial production of nuclease resistant DNA, RNA, and oligo mixtures
ATE431407T1 (de) * 2002-01-11 2009-05-15 Biospring Ges Fuer Biotechnolo Verfahren zur herstellung von dna
GB0205455D0 (en) * 2002-03-07 2002-04-24 Molecular Sensing Plc Nucleic acid probes, their synthesis and use
US6815167B2 (en) * 2002-04-25 2004-11-09 Geneohm Sciences Amplification of DNA to produce single-stranded product of defined sequence and length
GB0218089D0 (en) * 2002-08-03 2002-09-11 Epistem Ltd Target DNA
US20050118616A1 (en) * 2002-08-16 2005-06-02 Kawashima Tadashi R. Amplification of target nucleotide sequence without polymerase chain reaction
CA2496462C (en) * 2002-08-29 2014-01-28 Amersham Biosciences Corp Allele specific primer extension
JPWO2004061100A1 (ja) * 2002-12-10 2006-05-11 オリンパス株式会社 核酸の変異解析方法および遺伝子の発現解析方法
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
BRPI0416595B1 (pt) * 2003-11-15 2014-06-17 Basf Ag Substrato, e, proceso para produzir um acabamento provido com um substrato
WO2005073409A2 (en) * 2004-01-26 2005-08-11 Applera Corporation Methods, compositions, and kits for amplifying and sequencing polynucleotides
JP2005218385A (ja) * 2004-02-06 2005-08-18 Dnaform:Kk １本鎖ｄｎａを調製するための方法
WO2005080605A2 (en) 2004-02-19 2005-09-01 Helicos Biosciences Corporation Methods and kits for analyzing polynucleotide sequences
US8338578B1 (en) * 2004-03-05 2012-12-25 Quest Diagnostics Investments Incorporated Cystic fibrosis gene mutations
US7476734B2 (en) 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
US7635562B2 (en) 2004-05-25 2009-12-22 Helicos Biosciences Corporation Methods and devices for nucleic acid sequence determination
EP1623996A1 (de) * 2004-08-06 2006-02-08 Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts Verbessertes Verfahren zur Auswahl eines Proteins von einer Bibliothek
US7220549B2 (en) 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US7482120B2 (en) 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
JP2006296279A (ja) * 2005-04-20 2006-11-02 Sony Corp インターカレーターを用いるハイブリダイゼーション検出方法
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
WO2007070642A2 (en) * 2005-12-15 2007-06-21 Helicos Biosciences Corporation Methods for increasing accuracy of nucleic acid sequencing
US20080309926A1 (en) * 2006-03-08 2008-12-18 Aaron Weber Systems and methods for reducing detected intensity non uniformity in a laser beam
US7397546B2 (en) * 2006-03-08 2008-07-08 Helicos Biosciences Corporation Systems and methods for reducing detected intensity non-uniformity in a laser beam
US7897747B2 (en) * 2006-05-25 2011-03-01 The Board Of Trustees Of The Leland Stanford Junior University Method to produce single stranded DNA of defined length and sequence and DNA probes produced thereby
ES2665690T3 (es) 2007-02-02 2018-04-26 Genera Biosystems Limited Generación de moléculas de ácido nucleico
US7881933B2 (en) * 2007-03-23 2011-02-01 Verizon Patent And Licensing Inc. Age determination using speech
WO2009023676A1 (en) * 2007-08-12 2009-02-19 Integrated Dna Technologies, Inc. Microarray system with improved sequence specificity
US20090136916A1 (en) * 2007-08-13 2009-05-28 Trustees Of Tufts College Methods and microarrays for detecting enteric viruses
RU2453605C2 (ru) * 2007-09-14 2012-06-20 Игорь Эдуардович Грановский Дифференцирующий и специфический олигонуклеотиды для идентификации последовательностей днк трансгенных растений в пищевых продуктах, способ идентификации трансгенных продуктов, биочип, комбинация олигонуклеотидов (варианты) и набор для осуществления этого способа
US8211673B2 (en) * 2008-02-04 2012-07-03 Life Technologies Corporation Composition and method for sequencing nucleic acid
GB0803109D0 (en) * 2008-02-20 2008-03-26 Gene Bridges Gmbh Method of nucleic acid recombination
US8628940B2 (en) 2008-09-24 2014-01-14 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
WO2009120374A2 (en) 2008-03-28 2009-10-01 Pacific Biosciences Of California, Inc. Methods and compositions for nucleic acid sample preparation
EP4230747A3 (de) 2008-03-28 2023-11-15 Pacific Biosciences Of California, Inc. Zusammensetzungen und verfahren zur nukleinsäuresequenzierung
WO2010030776A1 (en) * 2008-09-10 2010-03-18 Genscript Corporation Homologous recombination-based dna cloning methods and compositions
WO2010036287A1 (en) 2008-09-24 2010-04-01 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
US8383369B2 (en) 2008-09-24 2013-02-26 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
WO2010078443A1 (en) 2008-12-31 2010-07-08 Abbott Point Of Care Inc. Method and device for immunoassay using nucleotide conjugates
US20120135414A1 (en) 2010-10-28 2012-05-31 Life Technologies Corporation Chemically-enhanced primer compositions, methods and kits
DE102012008375A1 (de) * 2011-04-27 2012-10-31 Genovoxx Gmbh Methoden und Komponenten zur Detektion von Nukleinsäureketten
WO2013059746A1 (en) 2011-10-19 2013-04-25 Nugen Technologies, Inc. Compositions and methods for directional nucleic acid amplification and sequencing
EP2807292B1 (de) 2012-01-26 2019-05-22 Tecan Genomics, Inc. Zusammensetzungen und verfahren zur gezielten nukleinsäuresequenzanreicherung und erzeugung einer hocheffizienten bibliothek
US9133509B2 (en) 2012-03-22 2015-09-15 Lgc Genomics Limited Polymerase chain reaction detection system
CA2877094A1 (en) 2012-06-18 2013-12-27 Nugen Technologies, Inc. Compositions and methods for negative selection of non-desired nucleic acid sequences
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
WO2014015098A1 (en) 2012-07-18 2014-01-23 Siemens Healthcare Diagnostics Inc. A method of normalizing biological samples
CN105026578B (zh) * 2013-03-05 2019-03-15 安捷伦科技有限公司 通过引物延伸合成探针库
US9822408B2 (en) 2013-03-15 2017-11-21 Nugen Technologies, Inc. Sequential sequencing
GB201312995D0 (en) * 2013-07-19 2013-09-04 Atlas Genetics Ltd Methods and kits for specific nucleic acid amplification and detection
US10351899B2 (en) 2013-09-25 2019-07-16 Bio-ID Diagnostics Inc. Methods for detecting nucleic acid fragments
WO2015073711A1 (en) 2013-11-13 2015-05-21 Nugen Technologies, Inc. Compositions and methods for identification of a duplicate sequencing read
US9745614B2 (en) 2014-02-28 2017-08-29 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
US10323273B2 (en) * 2014-06-05 2019-06-18 Qiagen Gmbh Optimization of DNA amplification reactions
CA2957633A1 (en) 2014-08-06 2016-02-11 Nugen Technologies, Inc. Digital measurements from targeted sequencing
GB2531615B (en) 2015-02-02 2017-11-22 Atlas Genetics Ltd Instrument for performing a diagnostic test on a fluidic cartridge
GB201501705D0 (en) 2015-02-02 2015-03-18 Atlas Genetics Ltd Instrument for performing a diagnostic test on a fluidic cartridge
GB2530596B (en) 2015-02-02 2016-08-24 Atlas Genetics Ltd Improved blister assembly
US10870879B2 (en) 2015-10-05 2020-12-22 Helmholtz Zentrum Münchendeutsches Forschungszentrum Für Gesundheit Und Umwelt Method for the preparation of bar-coded primer sets
US12492430B2 (en) 2017-04-11 2025-12-09 Tecan Genomics, Inc. Library quantitation and qualification
EP3692049A4 (de) * 2017-10-04 2021-06-23 Centrillion Technology Holdings Corporation Verfahren und system zur enzymatischen synthese von oligonukleotiden
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
CN111801427B (zh) * 2018-02-05 2023-12-05 豪夫迈·罗氏有限公司 用于单分子的单链环状dna模板的产生
CN113454238B (zh) 2019-01-25 2025-02-11 哈佛大学校长及研究员协会 用于合成核酸的组合物和方法
EP4073246A4 (de) 2019-12-12 2023-12-06 President And Fellows Of Harvard College Zusammensetzungen und verfahren zur lichtgerichteten biomolekularen barcodierung
EP3851542A1 (de) 2020-01-20 2021-07-21 Tecan Genomics, Inc. Verarmung von reichlich uninformativen sequenzen
US12059674B2 (en) 2020-02-03 2024-08-13 Tecan Genomics, Inc. Reagent storage system
CA3176545A1 (en) 2020-04-22 2021-10-28 President And Fellows Of Harvard College Isothermal methods, compositions, kits, and systems for detecting nucleic acids
EP4225946A4 (de) 2020-10-06 2024-10-30 President And Fellows Of Harvard College Verfahren, zusammensetzungen, kits und systeme für signal-on-nachweis von neutralisierungszielen
EP4729632A1 (de) 2024-10-18 2026-04-22 AskBio Inc. Verfahren zur synthese von dna

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4521509A (en) * 1982-11-24 1985-06-04 Research Corporation Method for degrading DNA
GB8311018D0 (en) * 1983-04-22 1983-05-25 Amersham Int Plc Detecting mutations in dna
US4683194A (en) * 1984-05-29 1987-07-28 Cetus Corporation Method for detection of polymorphic restriction sites and nucleic acid sequences
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
IL86724A (en) * 1987-06-19 1995-01-24 Siska Diagnostics Inc Methods and kits for amplification and testing of nucleic acid sequences
ATE92538T1 (de) * 1988-01-21 1993-08-15 Genentech Inc Verstaerkung und nachweis von nukleinsaeuresequenzen.
CA1340807C (en) * 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
JP2897959B2 (ja) * 1988-05-20 1999-05-31 エフ．ホフマン―ラ ロシュ アクチェンゲゼルシャフト 固定化された配列特異的プローブ
US5066584A (en) * 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
FR2650840B1 (fr) * 1989-08-11 1991-11-29 Bertin & Cie Procede rapide de detection et/ou d'identification d'une seule base sur une sequence d'acide nucleique, et ses applications

Also Published As

Publication number Publication date
JP2002209594A (ja) 2002-07-30
AU674211B2 (en) 1996-12-12
WO1994016090A1 (en) 1994-07-21
AU6126294A (en) 1994-08-15
EP0679190B1 (de) 2003-05-02
EP0679190A1 (de) 1995-11-02
DE69432586D1 (de) 2003-06-05
CA2153898C (en) 2003-10-28
JP3330946B2 (ja) 2002-10-07
EP0679190A4 (de) 1998-07-01
JP3421664B2 (ja) 2003-06-30
JPH08505535A (ja) 1996-06-18
US5518900A (en) 1996-05-21
DE69432586T2 (de) 2004-03-25
CA2153898A1 (en) 1994-07-21

Similar Documents

Publication Publication Date Title
ATE239090T1 (de) Methode zum generieren von einzelsträngigen dna molekülen
DE69627698T2 (de) Eine in kaskaden verlaufende vervielfältigungsreaktion von nukleinsäuren
ES2243007T3 (es) Sintesis quimica completa y sintesis de genes de genomas.
ATE225369T1 (de) Doppelsträngige peptidenukleinsäuren
PT92640A (pt) Method for making synthetic oligonucleotides which bind specifically to target sites on duplex dna molecules by forming a colinear triplex the synthetic oligonucleotides and methods of use
DK0698105T3 (da) Nukleotidsekvenser til styring af ekspressionen af DNAsekvenser i en værtscelle
DE69403642D1 (de) 7-deazapurin modifizierte oligonukleotide
EP0828849A4 (de) Oligonukleotidliganden mit hoher affinität für wachstumsfaktoren
DE69824716D1 (de) Methode zur sequenzierung von nukleinsäuren
ATE466948T1 (de) Dns-basiertes transposon-system für die einführung von nucleinsäure in die dns einer zelle
MX9400837A (es) Metodos y aparatos para la determinacion de secuencia de adn.
DE69841023D1 (de) DNA Polymerasen mit verbesserten Fähigkeiten zum Einbau von markierten Nukleotiden
ATE205504T1 (de) Peptidnukleinsäuren
EP1961826A3 (de) Verfahren zur direkten Sequenzierung von Nukleinsäuren
FI971611L (fi) DNA-polymeraaseja joilla on modifioitu nukleotidisitoutumiskohta DNA-sekvensointia varten
ATE266737T1 (de) Künstliche fehlhybridisierung
ATE236183T1 (de) Verfahren zur reinigung von nukleinsäuren
ATE217906T1 (de) Methoden zur zielrichtung von dna
EP1002126A4 (de) Verbessertes verfahren zur isolierung und wiedergewinnung von ziel-dna oder -rna molekülen mit einer gewünschten nukleotidsequenz
FI852625A7 (fi) Menetelmä kaksisäikeisten nukleiinihappomolekyylien muodostamiseksi yksisäikeisistä nukleiinihappomolekyyleistä.
ATE465261T1 (de) Integrin-gekoppelte kinase, ihre hemmstoffe und deren gebrauch für verfahren zur therapeutischen behandlung; gentherapie und bereitstellung von hemmstoffen, die als pseudosubstrate wirken
EP0769553A4 (de) Dna-fragment, rekombinanter vektor, der es enthält, und verfahren zur expression von fremdgenen mit seiner nutzung
ATE325189T1 (de) Klonierungsverfahren durch multiple verdauung
ITRM920438A0 (it) Sequenze nucleotidiche codificanti per una dna polimerasi.
MX9709596A (es) Adn catal ??tico.

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties