ATE217906T1 - Methoden zur zielrichtung von dna - Google Patents

Methoden zur zielrichtung von dna

Info

Publication number
ATE217906T1
ATE217906T1 AT92901270T AT92901270T ATE217906T1 AT E217906 T1 ATE217906 T1 AT E217906T1 AT 92901270 T AT92901270 T AT 92901270T AT 92901270 T AT92901270 T AT 92901270T AT E217906 T1 ATE217906 T1 AT E217906T1
Authority
AT
Austria
Prior art keywords
dna molecule
stranded dna
strand
double
methods
Prior art date
Application number
AT92901270T
Other languages
English (en)
Inventor
Rafael D Camerini-Otero
Margaret Mcintosh
Carol S Camerini-Otero
Lance J Ferrin
Original Assignee
Us Gov Health & Human Serv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Us Gov Health & Human Serv filed Critical Us Gov Health & Human Serv
Application granted granted Critical
Publication of ATE217906T1 publication Critical patent/ATE217906T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6839—Triple helix formation or other higher order conformations in hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/15—Nucleic acids forming more than 2 strands, e.g. TFOs
    • C12N2310/153—Nucleic acids forming more than 2 strands, e.g. TFOs with the aid of a protein, e.g. recombinase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT92901270T 1990-11-09 1991-11-08 Methoden zur zielrichtung von dna ATE217906T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US61126890A 1990-11-09 1990-11-09
US73374491A 1991-07-24 1991-07-24
PCT/US1991/008200 WO1992008791A1 (en) 1990-11-09 1991-11-08 Method of targeting dna

Publications (1)

Publication Number Publication Date
ATE217906T1 true ATE217906T1 (de) 2002-06-15

Family

ID=27086477

Family Applications (1)

Application Number Title Priority Date Filing Date
AT92901270T ATE217906T1 (de) 1990-11-09 1991-11-08 Methoden zur zielrichtung von dna

Country Status (8)

Country Link
US (1) US5460941A (de)
EP (1) EP0556330B1 (de)
JP (1) JP2739378B2 (de)
AT (1) ATE217906T1 (de)
AU (1) AU658386B2 (de)
CA (1) CA2095624C (de)
DE (1) DE69133018T2 (de)
WO (1) WO1992008791A1 (de)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020108136A1 (en) * 1997-03-21 2002-08-08 Sri Transgenic animals produced by homologous sequence targeting
AU4115693A (en) * 1992-04-24 1993-11-29 Sri International In vivo homologous sequence targeting in eukaryotic cells
US5861244A (en) * 1992-10-29 1999-01-19 Profile Diagnostic Sciences, Inc. Genetic sequence assay using DNA triple strand formation
US5731411A (en) * 1993-07-12 1998-03-24 The United States Of America As Represented By The Department Of Health And Human Services Promotion of homologous DNA pairing by RecA-derived peptides
AU7845194A (en) * 1993-09-28 1995-04-18 General Hospital Corporation, The Using antisense oligonucleotides to modulate nerve growth and to reverse beta/A4 amyloid-induced morphology
WO1995018236A1 (en) * 1993-12-28 1995-07-06 Daikin Industries, Ltd. IN-SITU HYBRIDIZATION METHOD USING RecA PROTEIN AND RecA PROTEIN HAVING MARKER OR LIGAND FOR USE IN SAID METHOD
US5780296A (en) * 1995-01-17 1998-07-14 Thomas Jefferson University Compositions and methods to promote homologous recombination in eukaryotic cells and organisms
US5776744A (en) * 1995-06-07 1998-07-07 Yale University Methods and compositions for effecting homologous recombination
JPH11509423A (ja) * 1995-07-21 1999-08-24 ザ ガヴァメント オヴ ザ ユナイテッド ステイツ オヴ アメリカ、リプレゼンティッド バイ ザ セクレタリー、ディパートメント オヴ ヘルス アンド ヒューマン サーヴィスィーズ Ｒｅｃ支援ＤＮＡクローニング
EP0864002A4 (de) * 1995-10-27 1999-07-28 Elliot R Ramberg Verfahren und zusammensetzungen zur erkennung von spezifischen nukleotidsequenzen
FR2757537B1 (fr) * 1996-12-23 1999-03-19 Genset Sa Procede destine a l'elimination de sequences specifiques lors de la production de banques d'adn
US5948653A (en) 1997-03-21 1999-09-07 Pati; Sushma Sequence alterations using homologous recombination
JPH11151100A (ja) * 1997-11-19 1999-06-08 Aisin Cosmos Kenkyusho:Kk プローブによる核酸の検出方法
CA2314246A1 (en) * 1997-12-11 1999-07-29 Pangene Corporation The use of consensus sequences for targeted homologous gene isolation and recombination in gene families
NL1009369C2 (nl) * 1998-06-10 1999-12-13 Stichting Tech Wetenschapp Werkwijze voor het onderdrukken van de expressie van een gen.
JP4265028B2 (ja) * 1999-04-14 2009-05-20 アイシン精機株式会社 二本鎖ｄｎａの特異的切断方法およびそのためのキット
JP4465741B2 (ja) * 1999-07-02 2010-05-19 アイシン精機株式会社 二本鎖ｄｎａ断片の末端での連結
US7045314B2 (en) * 2000-04-04 2006-05-16 Baxter International Inc. Method for the production of bacterial toxins
US6686180B2 (en) * 2000-04-04 2004-02-03 Baxter International Inc. Method for the production of bacterial toxins
US20020132350A1 (en) * 2000-09-14 2002-09-19 Pioneer Hi-Bred International, Inc. Targeted genetic manipulation using Mu bacteriophage cleaved donor complex
JP4681129B2 (ja) * 2001-01-19 2011-05-11 株式会社アイシン・コスモス研究所 核酸末端領域の非対称的修飾法
AU2002303176A1 (en) 2001-03-27 2002-10-15 University Of Delaware Genomics applications for modified oligonucleotides
US7468244B2 (en) 2001-09-27 2008-12-23 University Of Delaware Polymorphism detection and separation
WO2003089587A2 (en) * 2002-04-16 2003-10-30 Promega Corporation Method to enhance homologous recombination
JP4278412B2 (ja) 2003-03-13 2009-06-17 株式会社アイシン・コスモス研究所 部分的なｄｎａ鎖の相同的組換えによる遺伝子除去方法および遺伝子取得方法
ATE442445T1 (de) * 2003-10-03 2009-09-15 Promega Corp Rhamnose-induzierbares expressionssystem
US8293503B2 (en) 2003-10-03 2012-10-23 Promega Corporation Vectors for directional cloning
EP3985125A1 (de) 2004-06-01 2022-04-20 Abbott Diagnostics Scarborough, Inc. Rekombinase-polymerase-verstärkung
CN108026566A (zh) * 2015-05-04 2018-05-11 特拉维夫大学拉莫特有限公司 用于使dna片段化的方法和试剂盒
WO2018187779A1 (en) 2017-04-07 2018-10-11 Sage Science, Inc. Systems and methods for detection of genetic structural variation using integrated electrophoretic dna purification

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4888274A (en) * 1985-09-18 1989-12-19 Yale University RecA nucleoprotein filament and methods

Also Published As

Publication number Publication date
JP2739378B2 (ja) 1998-04-15
JPH06500926A (ja) 1994-01-27
DE69133018T2 (de) 2003-01-02
US5460941A (en) 1995-10-24
AU658386B2 (en) 1995-04-13
CA2095624A1 (en) 1992-05-10
AU9111691A (en) 1992-06-11
DE69133018D1 (de) 2002-06-27
EP0556330A1 (de) 1993-08-25
EP0556330B1 (de) 2002-05-22
CA2095624C (en) 2000-06-13
EP0556330A4 (en) 1995-02-08
WO1992008791A1 (en) 1992-05-29

Similar Documents

Publication Publication Date Title
DE69133018D1 (de) Methoden zur zielrichtung von dna
ATE239090T1 (de) Methode zum generieren von einzelsträngigen dna molekülen
DE69128545D1 (de) Technik des genetischen fingerabdrucks mit dns-vervielfältigung
DE69615411D1 (de) Verfahren zur Herstellung von Polynucleotiden mit erwünschten Eigenschaften mittels wiederholter Selektion und Rekombination
ATE225369T1 (de) Doppelsträngige peptidenukleinsäuren
DE60310944D1 (de) Weitere neue formen von interferierende rns moleküle
ATE165869T1 (de) Oligonukleotide zur amplifizierung von nukleinsäuresequenzen und zur anknüpfung einer promotersequenz
DE69433487D1 (de) Methoden und zusammensetzungen zur effizienten nukleinsaeuresequenzierung
DK0586474T3 (da) Peptionukleinsyrer
PT92640A (pt) Method for making synthetic oligonucleotides which bind specifically to target sites on duplex dna molecules by forming a colinear triplex the synthetic oligonucleotides and methods of use
DE69233458D1 (de) Nukleinsäuretypisierung durch polymeraseverlängerung von oligonukleotiden unter verwendung von terminator-mischungen
DE69636495D1 (de) Multi-tumor abweichende wachstumsgene
WO1998026093A3 (en) Fluorescent nucleotide analog hairpin formation for detection of nucleic acid hybridization
DE50213541D1 (de) Verfahren zur Herstellung von DNA
EP1341909B8 (de) Eine methode zur in vitro molekularen evolution der proteinfunktion
WO1997005276A3 (de) Verfahren zur sequenzierung durch oligomerhybridisierung
SE9503117D0 (sv) Novel use of padlock probes
CA2210769A1 (fr) Detection de bacteries du genre listeria selon des techniques d'hybridation de sondes nucleiques
DE69636725D1 (de) Verfahren zur trennung und/oder erkennung von dns-molekülen
DE69505338D1 (de) Nukleotidsequenzen die spezifisch mit einer genomischen Sequent von Campylobacter coli hybridisieren
FR2723591B1 (fr) Methode d'amplification et/ou de detection d'une sequence d'acide nucleique, reactif de detection et ses applications.
EP1221491B8 (de) System und verfahren zum sequenzieren genetischer substanzen
EP0320103A3 (de) Verbindungen zur DNS-Deletion und dazu verwendete Oligomere und eine Methode für ihre Anwendung
ES2190866A1 (es) Metodo para la insercion eficiente en sitios especificos de cualquier adn diana de intrones del grupo ii.

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties