ATE245701T1 - Modifizierte pilzzellen und methode zur herstelllung rekombinanter produkte - Google Patents

Modifizierte pilzzellen und methode zur herstelllung rekombinanter produkte

Info

Publication number
ATE245701T1
ATE245701T1 AT93919067T AT93919067T ATE245701T1 AT E245701 T1 ATE245701 T1 AT E245701T1 AT 93919067 T AT93919067 T AT 93919067T AT 93919067 T AT93919067 T AT 93919067T AT E245701 T1 ATE245701 T1 AT E245701T1
Authority
AT
Austria
Prior art keywords
pct
recombinant products
cells
producing recombinant
sec
Prior art date
Application number
AT93919067T
Other languages
English (en)
Inventor
Widmar Tanner
Sabine Strahl-Bolsinger
Reinhard Fleer
Alain Fournier
Original Assignee
Aventis Pharma Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aventis Pharma Sa filed Critical Aventis Pharma Sa
Application granted granted Critical
Publication of ATE245701T1 publication Critical patent/ATE245701T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT93919067T 1992-08-14 1993-08-16 Modifizierte pilzzellen und methode zur herstelllung rekombinanter produkte ATE245701T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE4226971A DE4226971C2 (de) 1992-08-14 1992-08-14 Modifizierte Pilzzellen und Verfahren zur Herstellung rekombinanter Produkte
PCT/EP1993/002179 WO1994004687A1 (en) 1992-08-14 1993-08-16 Modified fungal cells and method for producing recombinant products

Publications (1)

Publication Number Publication Date
ATE245701T1 true ATE245701T1 (de) 2003-08-15

Family

ID=6465579

Family Applications (1)

Application Number Title Priority Date Filing Date
AT93919067T ATE245701T1 (de) 1992-08-14 1993-08-16 Modifizierte pilzzellen und methode zur herstelllung rekombinanter produkte

Country Status (13)

Country Link
US (1) US5714377A (de)
EP (1) EP0658203B1 (de)
JP (1) JP3630424B2 (de)
AT (1) ATE245701T1 (de)
AU (1) AU679935B2 (de)
CA (1) CA2140894C (de)
DE (3) DE4244915C2 (de)
ES (1) ES2203619T3 (de)
FI (1) FI121075B (de)
NO (1) NO320349B1 (de)
NZ (1) NZ255350A (de)
WO (1) WO1994004687A1 (de)
ZA (1) ZA935915B (de)

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AU2007200859A1 (en) * 1999-01-30 2007-03-22 Novozymes Delta Limited Process
US20050100991A1 (en) * 2001-04-12 2005-05-12 Human Genome Sciences, Inc. Albumin fusion proteins
EP1274720A4 (de) * 2000-04-12 2004-08-18 Human Genome Sciences Inc Albumin enthaltende fusionsproteine
US6946134B1 (en) 2000-04-12 2005-09-20 Human Genome Sciences, Inc. Albumin fusion proteins
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US20050054051A1 (en) * 2001-04-12 2005-03-10 Human Genome Sciences, Inc. Albumin fusion proteins
US7507413B2 (en) 2001-04-12 2009-03-24 Human Genome Sciences, Inc. Albumin fusion proteins
US20050244931A1 (en) * 2001-04-12 2005-11-03 Human Genome Sciences, Inc. Albumin fusion proteins
US20060084794A1 (en) * 2001-04-12 2006-04-20 Human Genome Sciences, Inc. Albumin fusion proteins
CA2383556A1 (en) * 2001-05-16 2002-11-16 Pharmacia & Upjohn Company High-efficiency assay for protein mannosyl transferases
US7176278B2 (en) 2001-08-30 2007-02-13 Biorexis Technology, Inc. Modified transferrin fusion proteins
WO2003030821A2 (en) * 2001-10-05 2003-04-17 Human Genome Sciences, Inc. Albumin fusion proteins
EP1463752A4 (de) * 2001-12-21 2005-07-13 Human Genome Sciences Inc Albuminfusionsproteine
PT1463751E (pt) 2001-12-21 2013-08-26 Human Genome Sciences Inc Proteínas de fusão de albumina
CA2475382A1 (en) 2002-02-07 2003-08-14 Delta Biotechnology Limited Albumin-fused anti-angiogenesis peptides
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CA2513213C (en) 2003-01-22 2013-07-30 Human Genome Sciences, Inc. Albumin fusion proteins
WO2006067511A1 (en) 2004-12-23 2006-06-29 Novozymes Delta Limited Gene expression technique
GB0329722D0 (en) 2003-12-23 2004-01-28 Delta Biotechnology Ltd Modified plasmid and use thereof
US9057061B2 (en) 2003-12-23 2015-06-16 Novozymes Biopharma Dk A/S Gene expression technique
GB0329681D0 (en) 2003-12-23 2004-01-28 Delta Biotechnology Ltd Gene expression technique
JP5284789B2 (ja) * 2005-11-15 2013-09-11 グライコフィ， インコーポレイテッド Ｏ−グリコシル化を減少している糖タンパク質の生成
AU2012203729B2 (en) * 2005-11-22 2014-01-30 Glycofi, Inc Production of glycoproteins with reduced O-glycosylation
EP1816201A1 (de) * 2006-02-06 2007-08-08 CSL Behring GmbH Modifizierter Koagulationsfaktor VIIa mit verbesserter 'half-life'-Stabiltät
ES2553162T3 (es) * 2006-05-16 2015-12-04 Daiichi Sankyo Company, Limited Método de producción de alta secreción de proteínas
KR101193722B1 (ko) 2006-07-24 2013-01-11 바이오렉시스 파마슈티칼 코포레이션 엑센딘 융합 단백질
GB0711424D0 (en) * 2007-06-13 2007-07-25 Novozymes Delta Ltd Recombinant transferrin mutants
CA2695830A1 (en) 2007-08-08 2009-02-12 Novozymes Biopharma Dk A/S Transferrin variants and conjugates
US8637435B2 (en) * 2007-11-16 2014-01-28 Merck Sharp & Dohme Corp. Eukaryotic cell display systems
JP2011514157A (ja) 2008-02-20 2011-05-06 グライコフィ， インコーポレイテッド 蛋白質生産用ベクター及び酵母株
EP2263089B1 (de) * 2008-03-03 2015-01-28 GlycoFi, Inc. Oberflächenanzeige rekombinanter proteine bei niederen eukaryoten
EP2324121B1 (de) * 2008-08-12 2012-11-07 GlycoFi, Inc. Verbesserte vektoren und hefestämme zur proteinproduktion: ca2+-atpase-überexpression
US9120871B2 (en) 2009-01-23 2015-09-01 Novo Nordisk A/S Process for preparing FGF21 with low degree of O-glycosylation
WO2010089385A1 (en) 2009-02-06 2010-08-12 Novozymes Biopharma Dk A/S Purification process
WO2010092135A2 (en) 2009-02-11 2010-08-19 Novozymes Biopharma Uk Ltd. Albumin variants and conjugates
EP2401378B1 (de) 2009-02-25 2013-08-14 Merck Sharp & Dohme Corp. Stoffwechselmanipulation eines galaktoseassimilationswegs bei der glycoengineerten hefe pichia pastoris
CN102741280B (zh) 2009-10-30 2015-12-02 诺维信生物制药丹麦公司 白蛋白变体
US9528116B2 (en) 2009-10-30 2016-12-27 Merck Sharp & Dohme Corp. Method for producing therapeutic proteins in Pichia pastoris lacking dipeptidyl aminopeptidase activity
WO2011099028A1 (en) * 2010-02-10 2011-08-18 Biocon Limited Method of reducing glycosylation of proteins, processes and proteins thereof
KR101930961B1 (ko) 2010-02-24 2018-12-19 머크 샤프 앤드 돔 코포레이션 피키아 파스토리스에서 생산된 치료 당단백질 상의 ｎ-글리코실화 부위 점유를 증가시키는 방법
CN106977608A (zh) 2010-04-09 2017-07-25 阿尔布麦狄克斯公司 白蛋白衍生物和变体
RU2604811C2 (ru) 2010-05-27 2016-12-10 Мерк Шарп Энд Домэ Корп. Способ получения антител с улучшенными свойствами
JP5885191B2 (ja) 2011-01-04 2016-03-15 国立研究開発法人産業技術総合研究所 糖鎖改変酵母及びそれを用いた糖タンパク質の製造方法
KR20140114278A (ko) 2011-02-25 2014-09-26 머크 샤프 앤드 돔 코포레이션 변형된 ｏ-글리코실화를 갖는 단백질의 생산을 위한 효모 균주
JP2014517846A (ja) 2011-05-25 2014-07-24 メルク・シャープ・アンド・ドーム・コーポレーション 改善された特性を有するＦｃ含有ポリペプチドの製造方法
HK1200842A1 (en) 2011-11-18 2015-08-14 Albumedix Ltd Proteins with improved half-life and other properties
MX2014010278A (es) 2012-03-16 2015-03-05 Novozymes Biopharma Dk As Variantes de albumina.
WO2014072481A1 (en) 2012-11-08 2014-05-15 Novozymes Biopharma Dk A/S Albumin variants
US20160115216A1 (en) 2013-06-07 2016-04-28 Novo Nordisk A/S Method for Making Mature Insulin Polypeptides
MX364532B (es) 2013-07-04 2019-04-29 Glykos Finland Oy Celulas de hongos filamentosos deficientes de o-manosiltransferasa y metodos de uso de las mismas.
AU2015293949B2 (en) 2014-07-21 2019-07-25 Teknologian Tutkimuskeskus Vtt Oy Production of glycoproteins with mammalian-like N-glycans in filamentous fungi
WO2017029407A1 (en) 2015-08-20 2017-02-23 Albumedix A/S Albumin variants and conjugates

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MW3378A1 (en) * 1977-11-08 1980-03-12 Genentech Inc Method and means for microbial polypeptide expression;synthetic dna and process therefor
IT1203758B (it) * 1986-03-27 1989-02-23 Univ Roma Vettori di clonazione e di espressione di geni eterologhi in lieviti e lieviti trasformati con tali vettori
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FR2655660B1 (fr) * 1989-12-11 1992-03-20 Rhone Poulenc Sante Nouveaux polypeptides, sequences d'adn permettant leur expression, procede de preparation et leur utilisation.

Also Published As

Publication number Publication date
DE69333112D1 (de) 2003-08-28
ZA935915B (en) 1995-04-13
FI950628L (fi) 1995-02-13
NZ255350A (en) 1996-11-26
JP3630424B2 (ja) 2005-03-16
NO950534L (no) 1995-04-05
NO320349B1 (no) 2005-11-28
DE69333112T2 (de) 2004-06-09
ES2203619T3 (es) 2004-04-16
CA2140894A1 (en) 1994-03-03
AU4948293A (en) 1994-03-15
WO1994004687A1 (en) 1994-03-03
DE4244915C2 (de) 1998-12-03
JPH08500975A (ja) 1996-02-06
AU679935B2 (en) 1997-07-17
FI121075B (fi) 2010-06-30
DE4226971C2 (de) 1997-01-16
EP0658203B1 (de) 2003-07-23
NO950534D0 (no) 1995-02-13
EP0658203A1 (de) 1995-06-21
FI950628A0 (fi) 1995-02-13
DE4226971A1 (de) 1994-02-17
US5714377A (en) 1998-02-03
CA2140894C (en) 2010-03-02

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