ATE245701T1 - Modifizierte pilzzellen und methode zur herstelllung rekombinanter produkte - Google Patents

Modifizierte pilzzellen und methode zur herstelllung rekombinanter produkte

Info

Publication number
ATE245701T1
ATE245701T1 AT93919067T AT93919067T ATE245701T1 AT E245701 T1 ATE245701 T1 AT E245701T1 AT 93919067 T AT93919067 T AT 93919067T AT 93919067 T AT93919067 T AT 93919067T AT E245701 T1 ATE245701 T1 AT E245701T1
Authority
AT
Austria
Prior art keywords
pct
recombinant products
cells
producing recombinant
sec
Prior art date
Application number
AT93919067T
Other languages
English (en)
Inventor
Widmar Tanner
Sabine Strahl-Bolsinger
Reinhard Fleer
Alain Fournier
Original Assignee
Aventis Pharma Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aventis Pharma Sa filed Critical Aventis Pharma Sa
Application granted granted Critical
Publication of ATE245701T1 publication Critical patent/ATE245701T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT93919067T 1992-08-14 1993-08-16 Modifizierte pilzzellen und methode zur herstelllung rekombinanter produkte ATE245701T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE4226971A DE4226971C2 (de) 1992-08-14 1992-08-14 Modifizierte Pilzzellen und Verfahren zur Herstellung rekombinanter Produkte
PCT/EP1993/002179 WO1994004687A1 (en) 1992-08-14 1993-08-16 Modified fungal cells and method for producing recombinant products

Publications (1)

Publication Number Publication Date
ATE245701T1 true ATE245701T1 (de) 2003-08-15

Family

ID=6465579

Family Applications (1)

Application Number Title Priority Date Filing Date
AT93919067T ATE245701T1 (de) 1992-08-14 1993-08-16 Modifizierte pilzzellen und methode zur herstelllung rekombinanter produkte

Country Status (13)

Country Link
US (1) US5714377A (de)
EP (1) EP0658203B1 (de)
JP (1) JP3630424B2 (de)
AT (1) ATE245701T1 (de)
AU (1) AU679935B2 (de)
CA (1) CA2140894C (de)
DE (3) DE4244915C2 (de)
ES (1) ES2203619T3 (de)
FI (1) FI121075B (de)
NO (1) NO320349B1 (de)
NZ (1) NZ255350A (de)
WO (1) WO1994004687A1 (de)
ZA (1) ZA935915B (de)

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US20050100991A1 (en) * 2001-04-12 2005-05-12 Human Genome Sciences, Inc. Albumin fusion proteins
US6946134B1 (en) 2000-04-12 2005-09-20 Human Genome Sciences, Inc. Albumin fusion proteins
EP2213743A1 (de) 2000-04-12 2010-08-04 Human Genome Sciences, Inc. Albuminfusionsproteine
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US7507413B2 (en) 2001-04-12 2009-03-24 Human Genome Sciences, Inc. Albumin fusion proteins
US20050054051A1 (en) * 2001-04-12 2005-03-10 Human Genome Sciences, Inc. Albumin fusion proteins
US20050244931A1 (en) * 2001-04-12 2005-11-03 Human Genome Sciences, Inc. Albumin fusion proteins
US20060084794A1 (en) * 2001-04-12 2006-04-20 Human Genome Sciences, Inc. Albumin fusion proteins
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US7176278B2 (en) 2001-08-30 2007-02-13 Biorexis Technology, Inc. Modified transferrin fusion proteins
AU2002332041A1 (en) * 2001-10-05 2003-04-22 Human Genome Sciences, Inc. Albumin fusion proteins
WO2003059934A2 (en) * 2001-12-21 2003-07-24 Human Genome Sciences, Inc. Albumin fusion proteins
AU2002364586A1 (en) 2001-12-21 2003-07-30 Delta Biotechnology Limited Albumin fusion proteins
WO2005003296A2 (en) 2003-01-22 2005-01-13 Human Genome Sciences, Inc. Albumin fusion proteins
WO2003066824A2 (en) 2002-02-07 2003-08-14 Aventis Behring Gmbh Albumin-fused kunitz domain peptides
AU2003274102B2 (en) * 2002-12-20 2007-01-25 Unilever Ip Holdings B.V. Preparation of antifreeze protein
GB0329722D0 (en) 2003-12-23 2004-01-28 Delta Biotechnology Ltd Modified plasmid and use thereof
GB0329681D0 (en) 2003-12-23 2004-01-28 Delta Biotechnology Ltd Gene expression technique
WO2006067511A1 (en) 2004-12-23 2006-06-29 Novozymes Delta Limited Gene expression technique
US9057061B2 (en) 2003-12-23 2015-06-16 Novozymes Biopharma Dk A/S Gene expression technique
US8206949B2 (en) * 2005-11-15 2012-06-26 Glycofi, Inc. Production of glycoproteins with reduced O-glycosylation
AU2012203729B2 (en) * 2005-11-22 2014-01-30 Glycofi, Inc Production of glycoproteins with reduced O-glycosylation
EP1816201A1 (de) * 2006-02-06 2007-08-08 CSL Behring GmbH Modifizierter Koagulationsfaktor VIIa mit verbesserter 'half-life'-Stabiltät
AU2007250735A1 (en) 2006-05-16 2007-11-22 Daiichi Sankyo Company, Limited Method for high-level secretory production of protein
CN101511868B (zh) 2006-07-24 2013-03-06 比奥雷克西斯制药公司 毒蜥外泌肽融合蛋白
GB0711424D0 (en) * 2007-06-13 2007-07-25 Novozymes Delta Ltd Recombinant transferrin mutants
WO2009019314A1 (en) 2007-08-08 2009-02-12 Novozymes A/S Transferrin variants and conjugates
US8637435B2 (en) * 2007-11-16 2014-01-28 Merck Sharp & Dohme Corp. Eukaryotic cell display systems
US20100311122A1 (en) 2008-02-20 2010-12-09 Glycofi, Inc Vectors and yeast strains for protein production
JP5731827B2 (ja) 2008-03-03 2015-06-10 グライコフィ， インコーポレイテッド 下等真核生物中での組換えタンパク質の表面ディスプレイ
MX2011001706A (es) * 2008-08-12 2011-03-24 Glycofi Inc Vectores mejorados y cepas de levadura para produccion de proteina: sobreexpresion de ca2+ atpasa.
US9120871B2 (en) 2009-01-23 2015-09-01 Novo Nordisk A/S Process for preparing FGF21 with low degree of O-glycosylation
JP2012516878A (ja) 2009-02-06 2012-07-26 ノボザイムス バイオファーマ デーコー アクティーゼルスカブ 精製プロセス
KR101722961B1 (ko) 2009-02-11 2017-04-04 알부메딕스 에이/에스 알부민 변이체 및 접합체
AU2010218139B2 (en) 2009-02-25 2012-11-01 Merck Sharp & Dohme Corp. Metabolic engineering of a galactose assimilation pathway in the glycoengineered yeast Pichia pastoris
EP2493921B1 (de) 2009-10-30 2018-09-26 Albumedix Ltd Albuminvarianten
CN102725396A (zh) 2009-10-30 2012-10-10 默沙东公司 在缺乏二肽基氨肽酶活性的巴斯德毕赤酵母中生产治疗性蛋白的方法
MX2012009349A (es) * 2010-02-10 2013-02-11 Biocon Ltd Metodo para reducir glucosilacion de proteinas, procedimientos y proteinas de los mismos.
RU2012140429A (ru) 2010-02-24 2014-03-27 Мерк Шарп Энд Домэ Корп. Способ увеличения занятости участков n-ликозилирования на терапевтических гликопротеинах, продуцируемых в p pastoris
EP2556087A1 (de) 2010-04-09 2013-02-13 Novozymes Biopharma DK A/S Albuminderivate und varianten
JP5956982B2 (ja) 2010-05-27 2016-07-27 メルク・シャープ・エンド・ドーム・コーポレイション 改善された特性を有する抗体の製造方法
JP5885191B2 (ja) 2011-01-04 2016-03-15 国立研究開発法人産業技術総合研究所 糖鎖改変酵母及びそれを用いた糖タンパク質の製造方法
CN103764837A (zh) 2011-02-25 2014-04-30 默沙东公司 用于生产具有修饰的o-糖基化的蛋白的酵母菌株
AU2012258907A1 (en) 2011-05-25 2013-11-07 Merck Sharp & Dohme Corp. Method for preparing Fc-containing polypeptides having improved properties
EP2780364A2 (de) 2011-11-18 2014-09-24 Eleven Biotherapeutics, Inc. Proteine mit verbesserter halbwertzeit und anderen eigenschaften
DK2825556T3 (en) 2012-03-16 2018-04-16 Albumedix As albumin Variants
MX2015005363A (es) 2012-11-08 2015-11-06 Novozymes Biopharma Dk As Variantes de albumina.
JP2016521701A (ja) 2013-06-07 2016-07-25 ノヴォ ノルディスク アー／エス 成熟インスリンポリペプチドを作製するための方法
WO2015001049A1 (en) 2013-07-04 2015-01-08 Novartis Ag O-mannosyltransferase deficient filamentous fungal cells and methods of use thereof
SG11201700446XA (en) 2014-07-21 2017-02-27 Glykos Finland Oy Production of glycoproteins with mammalian-like n-glycans in filamentous fungi
JP7007261B2 (ja) 2015-08-20 2022-01-24 アルブミディクス リミティド アルブミン変異体及びコンジュゲート

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EP0001929B1 (de) * 1977-11-08 1989-04-19 Genentech, Inc. Plasmid zur Transformation eines bakteriellen Wirts um ihn zu Polypeptid Expression fähig zu machen
IT1203758B (it) * 1986-03-27 1989-02-23 Univ Roma Vettori di clonazione e di espressione di geni eterologhi in lieviti e lieviti trasformati con tali vettori
US4929553A (en) * 1987-05-29 1990-05-29 Canadian Patents & Development Ltd. Protease for specific processing of secreted proteins
FR2649991B2 (fr) * 1988-08-05 1994-03-04 Rhone Poulenc Sante Utilisation de derives stables du plasmide pkd1 pour l'expression et la secretion de proteines heterologues dans les levures du genre kluyveromyces
FR2655660B1 (fr) * 1989-12-11 1992-03-20 Rhone Poulenc Sante Nouveaux polypeptides, sequences d'adn permettant leur expression, procede de preparation et leur utilisation.

Also Published As

Publication number Publication date
ZA935915B (en) 1995-04-13
CA2140894C (en) 2010-03-02
AU679935B2 (en) 1997-07-17
DE69333112T2 (de) 2004-06-09
CA2140894A1 (en) 1994-03-03
ES2203619T3 (es) 2004-04-16
FI121075B (fi) 2010-06-30
DE4226971A1 (de) 1994-02-17
FI950628L (fi) 1995-02-13
FI950628A0 (fi) 1995-02-13
NZ255350A (en) 1996-11-26
EP0658203A1 (de) 1995-06-21
NO950534L (no) 1995-04-05
DE69333112D1 (de) 2003-08-28
WO1994004687A1 (en) 1994-03-03
JP3630424B2 (ja) 2005-03-16
JPH08500975A (ja) 1996-02-06
AU4948293A (en) 1994-03-15
NO950534D0 (no) 1995-02-13
NO320349B1 (no) 2005-11-28
US5714377A (en) 1998-02-03
EP0658203B1 (de) 2003-07-23
DE4244915C2 (de) 1998-12-03
DE4226971C2 (de) 1997-01-16

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