ATE246702T1 - Verfahren zur darstellung von oligonukleotiden - Google Patents

Verfahren zur darstellung von oligonukleotiden

Info

Publication number
ATE246702T1
ATE246702T1 AT94914046T AT94914046T ATE246702T1 AT E246702 T1 ATE246702 T1 AT E246702T1 AT 94914046 T AT94914046 T AT 94914046T AT 94914046 T AT94914046 T AT 94914046T AT E246702 T1 ATE246702 T1 AT E246702T1
Authority
AT
Austria
Prior art keywords
image
oligonucleotides
shown below
preparing oligonucleotides
reaction
Prior art date
Application number
AT94914046T
Other languages
English (en)
Inventor
Robert L Letsinger
Sergei M Gryaznov
Original Assignee
Univ Northwestern
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Northwestern filed Critical Univ Northwestern
Application granted granted Critical
Publication of ATE246702T1 publication Critical patent/ATE246702T1/de

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Virology (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
AT94914046T 1993-04-12 1994-04-06 Verfahren zur darstellung von oligonukleotiden ATE246702T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US4603293A 1993-04-12 1993-04-12
PCT/US1994/003747 WO1994024143A1 (en) 1993-04-12 1994-04-06 Method of forming oligonucleotides

Publications (1)

Publication Number Publication Date
ATE246702T1 true ATE246702T1 (de) 2003-08-15

Family

ID=21941212

Family Applications (1)

Application Number Title Priority Date Filing Date
AT94914046T ATE246702T1 (de) 1993-04-12 1994-04-06 Verfahren zur darstellung von oligonukleotiden

Country Status (9)

Country Link
US (1) US5476930A (de)
EP (1) EP0695305B1 (de)
JP (2) JP4079875B2 (de)
AT (1) ATE246702T1 (de)
AU (1) AU684279B2 (de)
CA (1) CA2160016C (de)
DE (1) DE69433010T2 (de)
ES (1) ES2204913T3 (de)
WO (1) WO1994024143A1 (de)

Families Citing this family (152)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995001365A1 (en) * 1993-07-02 1995-01-12 Lynx Therapeutics, Inc. Synthesis of branched nucleic acids
US5830658A (en) * 1995-05-31 1998-11-03 Lynx Therapeutics, Inc. Convergent synthesis of branched and multiply connected macromolecular structures
US5888731A (en) * 1995-08-30 1999-03-30 Visible Genetics Inc. Method for identification of mutations using ligation of multiple oligonucleotide probes
US6335437B1 (en) * 1998-09-07 2002-01-01 Isis Pharmaceuticals, Inc. Methods for the preparation of conjugated oligomers
US7033753B1 (en) * 1999-01-15 2006-04-25 University Of Rochester Compositions and methods for nonenzymatic ligation of oligonucleotides and detection of genetic polymorphisms
AU2001265121A1 (en) * 2000-05-30 2001-12-11 Applera Corporation Methods for detecting target nucleic acids using coupled ligation and amplification
EP1317464B1 (de) * 2000-10-11 2009-05-13 Applera Corporation Fluoreszierende nukleobasekonjugate mit anionische linker
US6951720B2 (en) * 2001-05-10 2005-10-04 San Diego State University Foundation Use of phosphorothiolate polynucleotides in ligating nucleic acids
EP1401850A1 (de) 2001-06-20 2004-03-31 Nuevolution A/S Nukleosidderivate zur herstellung einer verbindungsbibliothek
DE10142643A1 (de) * 2001-08-31 2003-04-24 Clondiag Chip Tech Gmbh Detektion von Wechselwirkungen auf Sonden-Arrays
DE10147074A1 (de) * 2001-09-25 2003-05-08 Beru Ag Verfahren zum Betreiben einer aus mehreren Heizelementen bestehenden mehrstufigen elektrischen Heizung
US20030165935A1 (en) 2001-11-21 2003-09-04 Vann Charles S. Digital assay
DE20200926U1 (de) * 2002-01-23 2002-04-18 Hegenscheidt-MFD GmbH & Co. KG, 41812 Erkelenz Festwalzgerät einer Festwalzmaschine für Kurbelwellen
WO2003078627A2 (en) * 2002-03-15 2003-09-25 Nuevolution A/S A building block capable of functional entity transfer to nucleophil
AU2003214031A1 (en) 2002-03-15 2003-09-29 Nuevolution A/S An improved method for synthesising templated molecules
WO2003078050A2 (en) * 2002-03-15 2003-09-25 Nuevolution A/S A building block forming a c-c bond upon reaction
US10731151B2 (en) 2002-03-15 2020-08-04 Nuevolution A/S Method for synthesising templated molecules
US20050247001A1 (en) * 2002-03-15 2005-11-10 Nuevolution A/S Building block forming a c-c or a c-hetero atom bond uponreaction
US7749699B2 (en) * 2002-07-18 2010-07-06 The Board Of Trustees Of The Leland Stanford Junior University Detection of chemical ligation using fluorescence quenching leaving groups
EP1539980B1 (de) 2002-08-01 2016-02-17 Nuevolution A/S MEHRSTUFENSYNTHESE VON MIT MATRIZEN ASSOZIIERTE MOLEKüLEN
EP2348124B1 (de) 2002-10-30 2013-12-11 Nuevolution A/S Synthese bifunktioneller Komplexe
WO2004046343A2 (en) * 2002-11-19 2004-06-03 Applera Corporation Polynucleotide sequence detection assays and analysis
US20050053957A1 (en) * 2002-11-19 2005-03-10 Applera Corporation Polynucleotide sequence detection assays
WO2004056994A2 (en) 2002-12-19 2004-07-08 Nuevolution A/S Quasirandom structure and function guided synthesis methods
US20070026397A1 (en) 2003-02-21 2007-02-01 Nuevolution A/S Method for producing second-generation library
GB0304371D0 (en) * 2003-02-26 2003-04-02 Solexa Ltd DNA Sequence analysis
US7915201B2 (en) 2003-03-20 2011-03-29 Nuevolution A/S Ligational encoding of small molecules
WO2005001129A2 (en) * 2003-06-06 2005-01-06 Applera Corporation Mobility cassettes
CN100412332C (zh) * 2003-07-10 2008-08-20 雅马哈发动机株式会社 骑乘型车辆用发动机
US11118215B2 (en) 2003-09-18 2021-09-14 Nuevolution A/S Method for obtaining structural information concerning an encoded molecule and method for selecting compounds
DE602005017370D1 (de) 2004-03-22 2009-12-10 Nuevolution As Ligationscodierung unter verwendung von oligonukleotidbausteinen
AU2005299590B2 (en) 2004-10-27 2010-09-16 Cepheid Closed-system multi-stage nucleic acid amplification reactions
WO2006076650A2 (en) * 2005-01-12 2006-07-20 Applera Corporation Compositions, methods, and kits for selective amplification of nucleic acids
US20060160102A1 (en) 2005-01-18 2006-07-20 Hossein Fakhrai-Rad Identification of rare alleles by enzymatic enrichment of mismatched heteroduplexes
US7393665B2 (en) 2005-02-10 2008-07-01 Population Genetics Technologies Ltd Methods and compositions for tagging and identifying polynucleotides
JP2008535518A (ja) 2005-04-14 2008-09-04 アプレラ コーポレイション プソイドイソシトシン核酸塩基誘導体を含む３’改変オリゴヌクレオチド、およびプライマーまたはプローブとしてのその適用
EP3257949A1 (de) 2005-06-15 2017-12-20 Complete Genomics Inc. Nukleinsäureanalyse durch zufällige mischungen von nichtüberlappenden fragmenten
US20070087360A1 (en) * 2005-06-20 2007-04-19 Boyd Victoria L Methods and compositions for detecting nucleotides
CA2624896C (en) 2005-10-07 2017-11-07 Callida Genomics, Inc. Self-assembled single molecule arrays and uses thereof
PT3305900T (pt) 2005-12-01 2021-10-27 Nuevolution As Métodos de codificação enzimática para síntese eficaz de bibliotecas grandes
US8900828B2 (en) 2006-05-01 2014-12-02 Cepheid Methods and apparatus for sequential amplification reactions
CA2651815A1 (en) 2006-05-10 2007-11-22 Dxterity Diagnostics Detection of nucleic acid targets using chemically reactive oligonucleotide probes
US8119352B2 (en) * 2006-06-20 2012-02-21 Cepheld Multi-stage amplification reactions by control of sequence replication times
US20090036325A1 (en) * 2007-05-25 2009-02-05 Applera Corporation Directed assembly of amplicons to enhance read pairing signature with massively parallel short read sequencers
US8008010B1 (en) 2007-06-27 2011-08-30 Applied Biosystems, Llc Chimeric oligonucleotides for ligation-enhanced nucleic acid detection, methods and compositions therefor
EP2171097A2 (de) 2007-06-29 2010-04-07 Population Genetics Technologies LTD. Verfahren und zusammensetzungen zur isolierung der varianten von nukleinsäuresequenzen
CN101910399B (zh) * 2007-10-30 2015-11-25 考利达基因组股份有限公司 用于核酸高通量测序的装置
US9551026B2 (en) 2007-12-03 2017-01-24 Complete Genomincs, Inc. Method for nucleic acid detection using voltage enhancement
ES2638324T3 (es) 2009-02-13 2017-10-19 X-Chem, Inc. Métodos de creación y examen de bibliotecas codificadas por ADN
CA2757300C (en) 2009-04-01 2018-01-09 Dxterity Diagnostics Incorporated Chemical ligation dependent probe amplification (clpa)
US20120156728A1 (en) 2010-12-17 2012-06-21 Life Technologies Corporation Clonal amplification of nucleic acid on solid surface with template walking
WO2010133972A1 (en) 2009-05-22 2010-11-25 Population Genetics Technologies Ltd Sorting asymmetrically tagged nucleic acids by selective primer extension
EP3029141A1 (de) 2009-08-20 2016-06-08 Population Genetics Technologies Ltd. Zusammensetzungen und verfahren für intramolekulare neuanordnung von nukleinsäuren
US20120245041A1 (en) 2009-11-04 2012-09-27 Sydney Brenner Base-by-base mutation screening
JP2013511991A (ja) 2009-11-25 2013-04-11 クアンタライフ， インコーポレイテッド 遺伝子材料を検出する方法および組成物
US20130053253A1 (en) 2010-02-22 2013-02-28 Population Genetics Technologies Ltd Region of Interest Extraction and Normalization Methods
WO2011107887A2 (en) 2010-03-02 2011-09-09 Population Genetic Technologies Ltd. Methods for replicating polynucleotides with secondary structure
EP3540059A1 (de) 2010-04-16 2019-09-18 Nuevolution A/S Bifunktionelle komplexe und verfahren zur herstellung sowie verwendung derartiger komplexe
US8828688B2 (en) 2010-05-27 2014-09-09 Affymetrix, Inc. Multiplex amplification methods
EP2585593B1 (de) 2010-06-24 2017-03-01 Population Genetics Technologies Ltd. Verfahren zur herstellung und verewigung einer polynukleotid-bibliothek sowie zur extraktion relevanter bereiche daraus
ES2523140T3 (es) 2010-09-21 2014-11-21 Population Genetics Technologies Ltd. Aumento de la confianza en las identificaciones de alelos con el recuento molecular
US8725422B2 (en) 2010-10-13 2014-05-13 Complete Genomics, Inc. Methods for estimating genome-wide copy number variations
WO2013082164A1 (en) 2011-11-28 2013-06-06 Life Technologies Corporation Enhanced ligation reactions
ES2645953T3 (es) 2011-01-17 2017-12-11 Life Technologies Corporation Ligamiento enzimático de ácidos nucleicos
EP3567121B1 (de) 2011-01-17 2023-08-30 Life Technologies Corporation Arbeitsablauf zum nachweis von liganden mit nukleinsäuren
US9365897B2 (en) 2011-02-08 2016-06-14 Illumina, Inc. Selective enrichment of nucleic acids
AU2012214312A1 (en) 2011-02-09 2013-08-22 Bio-Rad Laboratories, Inc. Analysis of nucleic acids
US9745616B2 (en) 2011-05-17 2017-08-29 Dxterity Diagnostics Incorporated Methods and compositions for detecting target nucleic acids
DK2729580T3 (en) 2011-07-08 2015-12-14 Keygene Nv SEQUENCE BASED genotyping BASED ON OLIGONUKLEOTIDLIGERINGSASSAYS
IN2014CN02574A (de) 2011-09-07 2015-06-26 Chem Inc X
EP3604555B1 (de) 2011-10-14 2024-12-25 President and Fellows of Harvard College Sequenzierung durch strukturanordnung
US10837879B2 (en) 2011-11-02 2020-11-17 Complete Genomics, Inc. Treatment for stabilizing nucleic acid arrays
EP2798086B1 (de) * 2011-12-28 2018-09-05 Ricardo Mancebo Reagenzien und verfahren für eine autoligationskettenreaktion
US10202628B2 (en) 2012-02-17 2019-02-12 President And Fellows Of Harvard College Assembly of nucleic acid sequences in emulsions
WO2013124743A1 (en) 2012-02-22 2013-08-29 Population Genetics Technologies Ltd. Compositions and methods for intramolecular nucleic acid rearrangement ii
WO2013128281A1 (en) 2012-02-28 2013-09-06 Population Genetics Technologies Ltd Method for attaching a counter sequence to a nucleic acid sample
US9914967B2 (en) 2012-06-05 2018-03-13 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using DNA origami probes
US9628676B2 (en) 2012-06-07 2017-04-18 Complete Genomics, Inc. Imaging systems with movable scan mirrors
US9488823B2 (en) 2012-06-07 2016-11-08 Complete Genomics, Inc. Techniques for scanned illumination
CA2877094A1 (en) 2012-06-18 2013-12-27 Nugen Technologies, Inc. Compositions and methods for negative selection of non-desired nucleic acid sequences
SG10201907347YA (en) 2012-07-13 2019-09-27 X Chem Inc Dna-encoded libraries having encoding oligonucleotide linkages not readable by polymerases
US9092401B2 (en) 2012-10-31 2015-07-28 Counsyl, Inc. System and methods for detecting genetic variation
CA2876505A1 (en) 2012-07-17 2014-01-23 Counsyl, Inc. System and methods for detecting genetic variation
US9411930B2 (en) 2013-02-01 2016-08-09 The Regents Of The University Of California Methods for genome assembly and haplotype phasing
WO2014121091A1 (en) 2013-02-01 2014-08-07 The Regents Of The University Of California Methods for genome assembly and haplotype phasing
US9708658B2 (en) 2013-03-19 2017-07-18 New England Biolabs, Inc. Enrichment of target sequences
EP3597772A1 (de) 2013-04-17 2020-01-22 Agency For Science, Technology And Research Verfahren zur erzeugung erweiterter sequenzauslesungen
SG11201600853UA (en) 2013-08-05 2016-03-30 Twist Bioscience Corp De novo synthesized gene libraries
US10385335B2 (en) 2013-12-05 2019-08-20 Centrillion Technology Holdings Corporation Modified surfaces
CN106460032B (zh) 2013-12-05 2019-12-24 生捷科技控股公司 图案化阵列的制备
CN107002117B (zh) 2013-12-05 2021-12-10 生捷科技控股公司 核酸测序方法
EP3540074A1 (de) 2013-12-11 2019-09-18 The Regents of the University of California Verfahren zur markierung innerer regionen von nukleinsäuremolekülen
US11060139B2 (en) 2014-03-28 2021-07-13 Centrillion Technology Holdings Corporation Methods for sequencing nucleic acids
CN106879252B (zh) 2014-06-10 2020-07-17 德克斯特里蒂诊断公司 用于收集和稳定生物样品的装置及方法
CA2955382C (en) 2014-07-21 2023-07-18 Illumina, Inc. Polynucleotide enrichment using crispr-cas systems
US10526641B2 (en) 2014-08-01 2020-01-07 Dovetail Genomics, Llc Tagging nucleic acids for sequence assembly
CN114438174B (zh) 2014-11-11 2025-07-15 伊鲁米那股份有限公司 使用crispr-cas系统的多核苷酸扩增
US10233490B2 (en) 2014-11-21 2019-03-19 Metabiotech Corporation Methods for assembling and reading nucleic acid sequences from mixed populations
WO2016093838A1 (en) 2014-12-11 2016-06-16 New England Biolabs, Inc. Enrichment of target sequences
CA2975855C (en) 2015-02-04 2025-09-23 Twist Bioscience Corporation SYNTHETIC GENE COMPOSITIONS AND ASSEMBLY METHODS
US10669304B2 (en) 2015-02-04 2020-06-02 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
WO2016134034A1 (en) 2015-02-17 2016-08-25 Dovetail Genomics Llc Nucleic acid sequence assembly
US11807896B2 (en) 2015-03-26 2023-11-07 Dovetail Genomics, Llc Physical linkage preservation in DNA storage
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
EP3103885B1 (de) 2015-06-09 2019-01-30 Centrillion Technology Holdings Corporation Verfahren zur sequenzierung von nukleinsäuren
EP3350314A4 (de) 2015-09-18 2019-02-06 Twist Bioscience Corporation Oligonukleinsäurevariantenbibliotheken variante und synthese davon
KR102794025B1 (ko) 2015-09-22 2025-04-09 트위스트 바이오사이언스 코포레이션 핵산 합성을 위한 가요성 기판
CA3002740A1 (en) 2015-10-19 2017-04-27 Dovetail Genomics, Llc Methods for genome assembly, haplotype phasing, and target independent nucleic acid detection
CN115920796A (zh) 2015-12-01 2023-04-07 特韦斯特生物科学公司 功能化表面及其制备
SG11201807117WA (en) 2016-02-23 2018-09-27 Dovetail Genomics Llc Generation of phased read-sets for genome assembly and haplotype phasing
DK3455372T3 (da) 2016-05-11 2020-01-06 Illumina Inc Polynukleotidberigelse og -amplifikation ved anvendelse af argonautsystemer
KR102412442B1 (ko) 2016-05-13 2022-06-22 더브테일 제노믹스 엘엘씨 보존된 샘플로부터의 장범위 링키지 정보의 회수
US11708574B2 (en) 2016-06-10 2023-07-25 Myriad Women's Health, Inc. Nucleic acid sequencing adapters and uses thereof
US10144962B2 (en) 2016-06-30 2018-12-04 Grail, Inc. Differential tagging of RNA for preparation of a cell-free DNA/RNA sequencing library
EP3500672A4 (de) 2016-08-22 2020-05-20 Twist Bioscience Corporation De-novo-synthetisierte nukleinsäure-bibliotheken
US10417457B2 (en) 2016-09-21 2019-09-17 Twist Bioscience Corporation Nucleic acid based data storage
WO2018064486A1 (en) 2016-09-29 2018-04-05 Counsyl, Inc. Noninvasive prenatal screening using dynamic iterative depth optimization
GB2573069A (en) 2016-12-16 2019-10-23 Twist Bioscience Corp Variant libraries of the immunological synapse and synthesis thereof
WO2018136856A1 (en) 2017-01-23 2018-07-26 Massachusetts Institute Of Technology Multiplexed signal amplified fish via splinted ligation amplification and sequencing
US10968447B2 (en) 2017-01-31 2021-04-06 Myriad Women's Health, Inc. Methods and compositions for enrichment of target polynucleotides
WO2018144217A1 (en) 2017-01-31 2018-08-09 Counsyl, Inc. Methods and compositions for enrichment of target polynucleotides
JP2020507312A (ja) 2017-02-10 2020-03-12 ザイマージェン インコーポレイテッド 複数の宿主用の複数のｄｎａコンストラクトのアセンブリ及び編集のためのモジュラーユニバーサルプラスミド設計戦略
EP4556433A3 (de) 2017-02-22 2025-08-06 Twist Bioscience Corporation Nukleinsäurebasierte datenspeicherung
WO2018170169A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
CA3057589A1 (en) 2017-03-24 2018-09-27 Myriad Women's Health, Inc. Copy number variant caller
US20210371918A1 (en) 2017-04-18 2021-12-02 Dovetail Genomics, Llc Nucleic acid characteristics as guides for sequence assembly
WO2018231872A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
WO2019005763A1 (en) 2017-06-26 2019-01-03 Phase Genomics Inc. METHOD FOR REGROUPING DNA SEQUENCES
US11180804B2 (en) 2017-07-25 2021-11-23 Massachusetts Institute Of Technology In situ ATAC sequencing
US11407837B2 (en) 2017-09-11 2022-08-09 Twist Bioscience Corporation GPCR binding proteins and synthesis thereof
CN111565834B (zh) 2017-10-20 2022-08-26 特韦斯特生物科学公司 用于多核苷酸合成的加热的纳米孔
KR102804057B1 (ko) 2018-01-04 2025-05-07 트위스트 바이오사이언스 코포레이션 Dna 기반 디지털 정보 저장
EP3746566A1 (de) 2018-01-31 2020-12-09 Dovetail Genomics, LLC Probenvorbereitung zur wiederherstellung der dna-verknüpfung
CA3100739A1 (en) 2018-05-18 2019-11-21 Twist Bioscience Corporation Polynucleotides, reagents, and methods for nucleic acid hybridization
CA3107002A1 (en) 2018-08-15 2020-04-30 Zymergen Inc. Applications of crispri in high throughput metabolic engineering
WO2020139871A1 (en) 2018-12-26 2020-07-02 Twist Bioscience Corporation Highly accurate de novo polynucleotide synthesis
JP2022522668A (ja) 2019-02-26 2022-04-20 ツイスト バイオサイエンス コーポレーション 抗体を最適化するための変異体核酸ライブラリ
CN113766930B (zh) 2019-02-26 2025-07-22 特韦斯特生物科学公司 Glp1受体的变异核酸文库
US11332738B2 (en) 2019-06-21 2022-05-17 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
EP4545965A3 (de) 2019-06-27 2025-06-11 Dovetail Genomics, LLC Verfahren und zusammensetzungen für näherungsligation
JP2022548783A (ja) 2019-09-23 2022-11-21 ツイスト バイオサイエンス コーポレーション 単一ドメイン抗体のバリアント核酸ライブラリー
EP4034566A4 (de) 2019-09-23 2024-01-24 Twist Bioscience Corporation Variante nukleinsäurebibliotheken für crth2
BR112021018125A2 (pt) 2019-10-25 2021-11-16 Illumina Cambridge Ltd Métodos para gerar um molde de ácido nucleico de fita dupla de extremidade fechada assimétrico, para gerar um molde de ácido nucleico de fita dupla assimétrico a partir do dna tagmentado e de sequenciamento de dna tagmentado
BR112022011235A2 (pt) 2019-12-09 2022-12-13 Twist Bioscience Corp Bibliotecas de variantes de ácido nucleico para receptores de adenosina
AU2022232600A1 (en) 2021-03-09 2023-09-14 Illumina, Inc. Analyzing expression of protein-coding variants in cells
BR112023018152A2 (pt) 2021-03-09 2023-10-31 Illumina Cambridge Ltd Preparação de biblioteca genômica e ensaios epigenéticos direcionados com o uso de ribonucleoproteínas de cas-grna
US20240158839A1 (en) 2021-03-15 2024-05-16 President And Fellows Of Harvard College Methods for in situ sequencing
US20240299945A1 (en) 2021-09-03 2024-09-12 Elegen Corporation Multi-way bead-sorting devices, systems, and methods of use thereof using pressure sources
US20230392201A1 (en) 2022-06-06 2023-12-07 Element Biosciences, Inc. Methods for assembling and reading nucleic acid sequences from mixed populations
CN119968468A (zh) 2022-07-12 2025-05-09 拓扑基因股份有限公司 Dna芯片亚微米精度大规模复制方法
WO2025019313A1 (en) 2023-07-14 2025-01-23 The Broad Institute, Inc. Method for assembling gene libraries from a pool of oligonucleotides

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5237016A (en) * 1989-01-05 1993-08-17 Siska Diagnostics, Inc. End-attachment of oligonucleotides to polyacrylamide solid supports for capture and detection of nucleic acids

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AU684279B2 (en) 1997-12-11
DE69433010D1 (de) 2003-09-11
AU6626694A (en) 1994-11-08
CA2160016A1 (en) 1994-10-27
US5476930A (en) 1995-12-19
EP0695305A1 (de) 1996-02-07
JP4079875B2 (ja) 2008-04-23
CA2160016C (en) 2008-06-03
DE69433010T2 (de) 2004-06-09
JP3593344B2 (ja) 2004-11-24
JPH08508998A (ja) 1996-09-24
WO1994024143A1 (en) 1994-10-27
JP2004129672A (ja) 2004-04-30
EP0695305B1 (de) 2003-08-06
JP2005015490A (ja) 2005-01-20
EP0695305A4 (de) 1996-12-11
ES2204913T3 (es) 2004-05-01

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