ATE247707T1 - Verfahren zur herstellung einer immobilisierten enzympräparation und ihre verwendung - Google Patents

Verfahren zur herstellung einer immobilisierten enzympräparation und ihre verwendung

Info

Publication number
ATE247707T1
ATE247707T1 AT95909664T AT95909664T ATE247707T1 AT E247707 T1 ATE247707 T1 AT E247707T1 AT 95909664 T AT95909664 T AT 95909664T AT 95909664 T AT95909664 T AT 95909664T AT E247707 T1 ATE247707 T1 AT E247707T1
Authority
AT
Austria
Prior art keywords
enzyme
pct
immobilized enzyme
granulator
immobilized
Prior art date
Application number
AT95909664T
Other languages
English (en)
Inventor
Sven Pedersen
Anne Morkeberg Larsen
Per Aasmul
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of ATE247707T1 publication Critical patent/ATE247707T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18Carboxylic ester hydrolases (3.1.1)
    • C12N9/20Triglyceride splitting, e.g. by means of lipase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/14Enzymes or microbial cells immobilised on or in an inorganic carrier

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Inorganic Chemistry (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
AT95909664T 1994-02-21 1995-02-21 Verfahren zur herstellung einer immobilisierten enzympräparation und ihre verwendung ATE247707T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK20794 1994-02-21
PCT/DK1995/000076 WO1995022606A1 (en) 1994-02-21 1995-02-21 Method for production of an immobilized enzyme preparation and use of the immobilized enzyme preparation

Publications (1)

Publication Number Publication Date
ATE247707T1 true ATE247707T1 (de) 2003-09-15

Family

ID=8090950

Family Applications (1)

Application Number Title Priority Date Filing Date
AT95909664T ATE247707T1 (de) 1994-02-21 1995-02-21 Verfahren zur herstellung einer immobilisierten enzympräparation und ihre verwendung

Country Status (7)

Country Link
US (1) US5776741A (de)
EP (1) EP0746608B1 (de)
JP (1) JP3670284B2 (de)
AT (1) ATE247707T1 (de)
AU (1) AU1806595A (de)
DE (1) DE69531538T2 (de)
WO (1) WO1995022606A1 (de)

Families Citing this family (64)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6936289B2 (en) 1995-06-07 2005-08-30 Danisco A/S Method of improving the properties of a flour dough, a flour dough improving composition and improved food products
BR9609381A (pt) * 1995-06-27 1999-05-18 Unilever Nv Processo para a imobilização de uma enzima partícula sólida processos catalisados por uma enzima anfifílica para a interesterficação de mono- di- ou triglicerídeos catalisado por uma fosfo-lipase e para a remoção de fosfo-lipídeos de óleo de triglicerídeo
FI103207B (fi) * 1996-08-27 1999-05-14 Sune Backlund Immobilisoidun entsyymin sisältävä geeli
SK80799A3 (en) * 1996-12-19 2000-03-13 Unilever Nv Immobilized enzyme and its use for the processing of triglyceride oils
EP1433852B1 (de) 1997-04-09 2006-06-28 Danisco A/S Verwendung von Lipase zur Verbesserung von Teigen und Backwaren
US6156548A (en) 1997-12-23 2000-12-05 Novo Nordisk A/S Immobilization of enzymes with a fluidized bed for use in an organic medium
ATE254170T1 (de) * 1997-12-23 2003-11-15 Novozymes As Verfahren zur immobilisierung von enzymen
DK1098988T4 (da) 1998-07-21 2007-09-03 Danisco Födevare
CA2431972C (en) 2001-01-10 2012-10-23 Novozymes A/S Thermostable lipolytic enzyme variant
NZ528260A (en) 2001-05-18 2005-09-30 Danisco Method of improving dough and bread quality with the addition of an enzyme that hydrolyses a glycolipid and a phospholipid and incapable of hydrolysing a triglyceride or monoglyceride
FR2837204B1 (fr) * 2002-03-18 2004-07-09 Oreal Procede d'immobilation d'une proteine par extrusion continue
EP1403274A1 (de) * 2002-09-30 2004-03-31 Meristem Therapeutics Verfahren zur Reinigung von rekombinanten Proteinen aus komplexen Medien sowie die auf diese Weise erhaltenen gereinigten Proteine
EP3323887A3 (de) 2003-01-17 2018-09-12 DuPont Nutrition Biosciences ApS Verfahren zur vor-ort-herstellung einer emulsion in einem lebensmittel
US7955814B2 (en) * 2003-01-17 2011-06-07 Danisco A/S Method
US20050196766A1 (en) 2003-12-24 2005-09-08 Soe Jorn B. Proteins
MXPA05007654A (es) 2003-01-17 2005-09-30 Danisco Metodo.
US7642077B2 (en) * 2003-12-08 2010-01-05 Genencor International, Inc. Biocomposite comprising co-precipitate of enzyme, silicate and polyamine
US7718408B2 (en) 2003-12-24 2010-05-18 Danisco A/S Method
US7906307B2 (en) 2003-12-24 2011-03-15 Danisco A/S Variant lipid acyltransferases and methods of making
GB0716126D0 (en) 2007-08-17 2007-09-26 Danisco Process
GB0405637D0 (en) 2004-03-12 2004-04-21 Danisco Protein
EA014985B1 (ru) 2004-07-16 2011-04-29 Даниско А/С Способ ферментативного рафинирования пищевых масел гидратацией и применение липидацилтрансферазы для осуществления способа
AU2006256131B2 (en) * 2005-06-09 2010-09-23 Novozymes A/S Lipase powder composition
BRPI0615897B1 (pt) 2005-09-12 2022-05-31 Novozymes North America, Inc. Processo para interesterificar óleo contendo um ou mais agentes quelantes de metal
US9303256B2 (en) 2005-09-30 2016-04-05 Novozymes A/S Immobilization of enzymes
UA97127C2 (uk) * 2006-12-06 2012-01-10 Бандж Ойлз, Инк. Спосіб безперервної ферментативної обробки композиції, що містить ліпід, та система для його здійснення
WO2008072382A1 (ja) 2006-12-15 2008-06-19 Kao Corporation 固定化酵素を用いた有用物質の製造方法
AU2007344910B2 (en) * 2007-01-25 2013-03-28 Dupont Nutrition Biosciences Aps Production of a lipid acyltransferase from transformed Bacillus licheniformis cells
EP2126090B1 (de) * 2007-01-31 2015-04-01 Novozymes A/S Wärmestabile kohlenstoffanhydrasen und ihre verwendung
US9416383B2 (en) * 2007-04-27 2016-08-16 University Of North Carolina At Chapel Hill Method for enhancing catalytic activity of a lipase
CN104212844A (zh) 2007-06-01 2014-12-17 索拉兹米公司 在微生物中生产油
DE102007027206A1 (de) * 2007-06-13 2008-12-18 Süd-Chemie AG Immobilisierung von Enzymen auf Bleicherden
EP2370554B1 (de) 2008-11-28 2019-05-15 Corbion Biotech, Inc. Herstellung von massgeschneiderten ölen in rekombinanten heterotrophen mikroorganismen
US20100297749A1 (en) * 2009-04-21 2010-11-25 Sapphire Energy, Inc. Methods and systems for biofuel production
CN102712919B (zh) 2009-06-26 2017-06-23 诺维信北美公司 热稳定性碳酸酐酶及其用途
EP2576800B1 (de) 2010-05-28 2019-01-30 Corbion Biotech, Inc. Verfahren zur Herstellung von Ölen aus Prototheca
US20130149771A1 (en) 2010-08-24 2013-06-13 Novozymes A/S Heat-Stable Persephonella Carbonic Anhydrases and Their Use
IT1403355B1 (it) 2010-12-23 2013-10-17 Univ Degli Studi Trieste Metodo per l'immobilizzazione covalente di enzimi su supporti polimerici solidi funzionalizzati
AU2012212079B2 (en) 2011-02-02 2016-07-28 Corbion Biotech, Inc. Tailored oils produced from recombinant oleaginous microorganisms
US9410140B2 (en) 2011-05-10 2016-08-09 Danisco Us Inc Thermostable carbonic anhydrases and methods of use thereof
RU2528778C2 (ru) * 2011-11-11 2014-09-20 Российская Федерация, От Имени Которой Выступает Министерство Промышленности И Торговли Российской Федерации Биокатализатор для переэтерификации жиров и способ его получения
EP2839018B1 (de) 2012-04-18 2019-06-05 Corbion Biotech, Inc. Massgeschneiderte öle
WO2014067933A1 (en) 2012-10-31 2014-05-08 C-Lecta Gmbh Bioactive carrier preparation for enhanced safety in care products and food
US9816079B2 (en) 2013-01-29 2017-11-14 Terravia Holdings, Inc. Variant thioesterases and methods of use
US9567615B2 (en) 2013-01-29 2017-02-14 Terravia Holdings, Inc. Variant thioesterases and methods of use
BR102013003688A2 (pt) * 2013-02-18 2013-10-22 Prozyn Ind E Com Ltda Processo de obtenção de melhoradores de farinhas de trigo na forma de pó que incorporam enzimas líquidas e emulsificantes líquidos previamente tratados enzimaticamente em adsorventes de grau alimentício
US9290749B2 (en) 2013-03-15 2016-03-22 Solazyme, Inc. Thioesterases and cells for production of tailored oils
CN105189776A (zh) 2013-03-15 2015-12-23 诺维信北美公司 用于分析co2吸收的组合物和方法
US9783836B2 (en) 2013-03-15 2017-10-10 Terravia Holdings, Inc. Thioesterases and cells for production of tailored oils
US9249252B2 (en) 2013-04-26 2016-02-02 Solazyme, Inc. Low polyunsaturated fatty acid oils and uses thereof
CN105829521A (zh) 2013-10-04 2016-08-03 索拉兹米公司 定制油
DE102014203964A1 (de) 2014-03-05 2015-09-10 Evonik Degussa Gmbh Granulat enthaltend Isomaltulose Synthase
EP3660149A1 (de) 2014-07-24 2020-06-03 Corbion Biotech, Inc. Variante thioesterasen und verfahren zur verwendung
CN107208103A (zh) 2014-09-18 2017-09-26 泰拉瑞亚控股公司 酰基‑acp硫酯酶及其突变体
BR112017021421A2 (pt) 2015-04-06 2018-07-24 Terravia Holdings Inc microalgas oleaginosas que têm uma ablação de lpaat
CN109477083B (zh) 2016-07-20 2023-06-06 诺维信公司 热稳定性宏基因组碳酸酐酶及其用途
RU2020120682A (ru) 2017-11-24 2021-12-24 Новозимс А/С Мелкие частицы на основе фермента для переэтерификации
EP3744838A1 (de) 2019-05-29 2020-12-02 Novozymes A/S Lipolytische polymerpartikel zur veresterung und umesterung
CN111172203B (zh) * 2019-12-24 2021-11-23 北京理工大学 一种可大大加速酶催化二氧化碳转化为甲酸反应的方法
CN112028079B (zh) * 2020-08-31 2021-11-19 山西大学 一种载酶氧化硅毫米球及其制备方法和应用
WO2022180273A1 (en) 2021-02-26 2022-09-01 Novozymes A/S Polystyrene/divinylbenzene particles for lipase immobilization
EP4627074A2 (de) 2022-11-30 2025-10-08 Novozymes A/S Carboanhydrasevarianten und polynukleotide zur codierung davon
WO2025083001A2 (en) 2023-10-16 2025-04-24 Novozymes A/S Method of producing interesterified fat product
WO2025168806A1 (en) 2024-02-08 2025-08-14 Novozymes A/S Polypeptides having carbonic anhydrase activity and polynucleotides encoding same

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1577933A (en) * 1976-02-11 1980-10-29 Unilever Ltd Fat process and composition
EP0093027A1 (de) * 1982-04-27 1983-11-02 ARGILES & MINERAUX AGS-BMP Träger für die Fixierung von Mikroorganismen
DK402583D0 (da) * 1983-09-05 1983-09-05 Novo Industri As Fremgangsmade til fremstilling af et immobiliseret lipasepraeparat og anvendelse deraf
US4689315A (en) * 1984-04-14 1987-08-25 Redco N.V. Amorphous silica particles, a method for producing same, and catalyst thereof
JP2628667B2 (ja) * 1986-10-17 1997-07-09 ノボ ノルディスク アクティーゼルスカブ 位置非特異性リパーゼ
JP2641935B2 (ja) * 1987-09-28 1997-08-20 ノボ‐ノルディスク アクティーゼルスカブ リパーゼの固定化方法
DK638688D0 (da) * 1988-11-16 1988-11-16 Novo Industri As Partikelformet immobiliseret lipase-praeparat, fremgangsmaade til fremstilling deraf og anvendelse deraf
US5445955A (en) * 1992-05-25 1995-08-29 The Nisshin Oil Mills, Ltd. Immobilization of lipase on a polymer carrier containing epoxy and tertiary amino groups
EP0675952A1 (de) * 1993-05-18 1995-10-11 Genencor International, Inc. Staubfreies herstellungsverfahren für enzymen

Also Published As

Publication number Publication date
WO1995022606A1 (en) 1995-08-24
EP0746608A1 (de) 1996-12-11
DE69531538D1 (de) 2003-09-25
EP0746608B1 (de) 2003-08-20
JPH09508803A (ja) 1997-09-09
US5776741A (en) 1998-07-07
AU1806595A (en) 1995-09-04
DE69531538T2 (de) 2004-06-24
JP3670284B2 (ja) 2005-07-13

Similar Documents

Publication Publication Date Title
DE69531538D1 (de) Verfahren zur herstellung einer immobilisierten enzympräparation und ihre verwendung
US5156963A (en) Immobilization of lipase by adsorption on a particulate macroporous resin
DE69512817T2 (de) In Sol-Gel immobilizierte Lipase bearbeitet hydrophoben Substanzen
DE59504274D1 (de) Herstellung eines mehrenzymgranulats
ID18399A (id) Proses untuk pembuatan serat-serat berikatan silang asam polikarboksilat yang terpisah-pisah dengan bau yang berkurang dan kecermerlangan yang ditingkatkan
ATE254170T1 (de) Verfahren zur immobilisierung von enzymen
ATE160582T1 (de) Verfahren zur herstellung immobilisierter lipasen
CN101790582A (zh) 对于有机介质中的亲水性底物具有高耐受性的改性的固定化酶
JPH066058B2 (ja) 酵素剤の製造法
EP0321777A3 (en) Process for the transesterification of fat and oil
DE69603192D1 (de) Photokatalysator und verfahren zu seiner herstellung
RU2008148843A (ru) Способ восстановления липазной активности
US5232843A (en) Preparation of immobilized lipase by adsorption of lipase and a non-lipase protein on a support
DE69506900D1 (de) Enzymatisches verfahren zur herstellung von chiralen alpha-tertiären carbonsäureestern
DE69528903D1 (de) Verfahren zur kalziumphosphat-transfektion
EP0354551A3 (de) Hitzestabile Lipoproteinlipase, Verfahren zu deren Herstellung sowie ein Reagenz zur Bestimmung von Triglyceriden, welches diese Lipase enthält
ATE47714T1 (de) Verfahren zur herstellung von n1-methyl-10 alpha- methoxylumilysergol und seine ester, und zwischenverbindungen fuer ihre herstellung.
ATE207868T1 (de) Verfahren zur herstellung von dialkylcarbonaten
JP2657887B2 (ja) 固定化酵素の調製方法
JP2886902B2 (ja) 酵素含有物及びそれを用いた固定化酵素
WO1991003564A1 (en) Interesterification of phospholipids
JPS63214184A (ja) 固定化酵素およびその製造方法
EP1637606A3 (de) Triacylglycerol-Lipasen
JPH07265073A (ja) 高エステル交換活性酵素剤およびその製造方法
JPS6322795B2 (de)

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties