ATE249516T1 - Nukleinsäure-isolierung - Google Patents

Nukleinsäure-isolierung

Info

Publication number
ATE249516T1
ATE249516T1 AT95900235T AT95900235T ATE249516T1 AT E249516 T1 ATE249516 T1 AT E249516T1 AT 95900235 T AT95900235 T AT 95900235T AT 95900235 T AT95900235 T AT 95900235T AT E249516 T1 ATE249516 T1 AT E249516T1
Authority
AT
Austria
Prior art keywords
nucleic acid
pct
sec
sample
date
Prior art date
Application number
AT95900235T
Other languages
English (en)
Inventor
Jarle Breivik
Gustav Gaudernack
Anne Spurkland
Original Assignee
Qiagen As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qiagen As filed Critical Qiagen As
Application granted granted Critical
Publication of ATE249516T1 publication Critical patent/ATE249516T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
AT95900235T 1993-11-11 1994-11-10 Nukleinsäure-isolierung ATE249516T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB939323305A GB9323305D0 (en) 1993-11-11 1993-11-11 Isoaltion of nucleic acid
PCT/GB1994/002469 WO1995013368A1 (en) 1993-11-11 1994-11-10 Isolation of nucleic acid

Publications (1)

Publication Number Publication Date
ATE249516T1 true ATE249516T1 (de) 2003-09-15

Family

ID=10745030

Family Applications (1)

Application Number Title Priority Date Filing Date
AT95900235T ATE249516T1 (de) 1993-11-11 1994-11-10 Nukleinsäure-isolierung

Country Status (9)

Country Link
US (1) US6090935A (de)
EP (1) EP0728198B1 (de)
AT (1) ATE249516T1 (de)
AU (1) AU682074B2 (de)
DE (1) DE69433136T2 (de)
DK (1) DK0728198T3 (de)
ES (1) ES2206485T3 (de)
GB (1) GB9323305D0 (de)
WO (1) WO1995013368A1 (de)

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JP2965131B2 (ja) 1995-07-07 1999-10-18 東洋紡績株式会社 核酸結合用磁性担体およびそれを用いる核酸単離方法
US8048629B2 (en) 1996-03-15 2011-11-01 The Penn State Research Foundation Detection of extracellular tumor-associated nucleic acid in blood plasma or serum
US6156504A (en) * 1996-03-15 2000-12-05 The Penn State Research Foundation Detection of extracellular tumor-associated nucleic acid in blood plasma or serum using nucleic acid amplification assays
ES2126513B1 (es) * 1997-03-14 1999-12-01 Consejo Superior Investigacion Procedimiento para la deteccion de hongos y bacterias en conductos de aire acondicionado.
US6914137B2 (en) 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
US5973138A (en) 1998-10-30 1999-10-26 Becton Dickinson And Company Method for purification and manipulation of nucleic acids using paramagnetic particles
AU2001262152A1 (en) * 2000-03-31 2001-10-08 Memorec Stoffel Gmbh Method for extracting nucleic acids
WO2001089705A2 (en) * 2000-05-19 2001-11-29 Becton Dickinson And Company System and method for manipulating magnetically responsive particles in fluid samples to collect dna or rna from a sample
US6672458B2 (en) 2000-05-19 2004-01-06 Becton, Dickinson And Company System and method for manipulating magnetically responsive particles fluid samples to collect DNA or RNA from a sample
US20030087286A1 (en) * 2000-09-06 2003-05-08 Hodge Timothy A. Isolation of eukaryotic genomic DNA using magnetically responsive solid functionalized particles
US7011943B2 (en) 2000-09-06 2006-03-14 Transnetyx, Inc. Method for detecting a designated genetic sequence in murine genomic DNA
CN1250740C (zh) * 2000-09-06 2006-04-12 蒂莫西·A·霍奇 用于转基因及定向诱变筛选的系统和方法
US7494817B2 (en) * 2000-09-06 2009-02-24 Transnet Yx, Inc. Methods for genotype screening using magnetic particles
US20050239125A1 (en) * 2000-09-06 2005-10-27 Hodge Timothy A Methods for genotype screening
US20050272085A1 (en) * 2000-09-06 2005-12-08 Hodge Timothy A Methods for forensic and congenic screening
US20030082605A1 (en) * 2000-09-06 2003-05-01 Hodge Timothy A. Genomic DNA detection method and system thereof
US20050266494A1 (en) * 2000-09-06 2005-12-01 Hodge Timothy A System and method for computer network ordering of biological testing
US20030073110A1 (en) * 2001-07-03 2003-04-17 Masaharu Aritomi Method for isolating nucleic acid and a cartridge for chemical reaction and for nucleic acid isolation
US20060014186A1 (en) * 2001-09-04 2006-01-19 Hodge Timothy A Methods for genotype screening of a strain disposed on an adsorbent carrier
GB0212826D0 (en) 2002-05-31 2002-07-10 Dna Res Innovations Ltd Materials and methods relating to polyions and substance delivery
CA2531105C (en) * 2003-07-05 2015-03-17 The Johns Hopkins University Method and compositions for detection and enumeration of genetic variations
JP4476050B2 (ja) * 2004-06-30 2010-06-09 株式会社ニデック 視野計
EP1776458A2 (de) 2004-07-30 2007-04-25 Agencourt Bioscience Corporation Verfahren zur isolierung von nukleinsäuren mit hilfe von festphasenträgern mit multifunktionalen gruppen
US20090215124A1 (en) 2005-02-15 2009-08-27 Weidong Cao Nucleic acid isolation methods and materials and devices thereof
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
JP5643650B2 (ja) 2007-11-21 2014-12-17 コスモシド・インコーポレーテッド ゲノム同定システム
US8478544B2 (en) 2007-11-21 2013-07-02 Cosmosid Inc. Direct identification and measurement of relative populations of microorganisms with direct DNA sequencing and probabilistic methods
DE102008010693A1 (de) 2008-02-22 2009-08-27 Qiagen Gmbh Neue Matrixmaterialien, Verfahren zu ihrer Herstellung und ihre Verwendung in Verfahren zur Isolierung von Biomolekülen
DE102008010692A1 (de) 2008-02-22 2009-08-27 Qiagen Gmbh Neue Matrices, Verfahren zu ihrer Herstellung und ihre Verwendung in Verfahren zur Isolierung von Biomolekülen
RU2372406C1 (ru) * 2008-02-27 2009-11-10 Государственное образовательное учреждение высшего профессионального образования "Пермская государственная медицинская академия имени академика Е.А. Вагнера Федерального агентства по здравоохранению и социальному развитию" Способ выявления обсемененности объектов внешней среды грамотрицательными бактериями рода pseudomonas и acinetobacter
WO2010028382A2 (en) * 2008-09-05 2010-03-11 Mayo Foundation For Medical Education And Research Collecting and processing complex macromolecular mixtures
DE102008055120A1 (de) * 2008-12-23 2010-07-01 Qiagen Gmbh Präparation und Amplifikation von Nukleinsäuren mittels magnetischer Partikel
US8574835B2 (en) 2009-05-29 2013-11-05 Life Technologies Corporation Scaffolded nucleic acid polymer particles and methods of making and using
TWI527245B (zh) 2010-07-03 2016-03-21 生命技術公司 具有微摻雜汲極之化學感測器
TWI425977B (zh) 2010-11-24 2014-02-11 Ind Tech Res Inst 將福馬林固定石蠟包埋之組織進行脫蠟的方法與其套組
AU2012242847B2 (en) 2011-04-15 2017-01-19 The Johns Hopkins University Safe sequencing system
CN102229927B (zh) * 2011-06-03 2016-03-23 聂棱 提高案件微量检材脱落细胞dna提取效率的方法及试剂
EP2891716B1 (de) 2012-08-28 2018-03-28 Biocube System Inc. Poröse festphase zur schnellen isolierung biologischer moleküle für eine nukleinsäureamplifizierungsreaktion aus biologischen proben und verwendung davon
ES2701742T3 (es) 2012-10-29 2019-02-25 Univ Johns Hopkins Prueba de Papanicolaou para cánceres de ovario y de endometrio
WO2017027653A1 (en) 2015-08-11 2017-02-16 The Johns Hopkins University Assaying ovarian cyst fluid
CN120400338A (zh) 2017-08-07 2025-08-01 约翰斯霍普金斯大学 用于评估和治疗癌症的方法和材料
US20200208137A1 (en) 2017-09-18 2020-07-02 Yin To Chiu Method for Using Aqueous Two-Phase System for the Isolation, Purification and/or Concentration of Short Nucleic Acid Fragments
IL295297A (en) 2020-02-14 2022-10-01 Univ Johns Hopkins Methods and materials for assessing nucleic acids

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AU530410B2 (en) * 1978-02-21 1983-07-14 Sintef Preparing aqueous emulsions
NO155316C (no) * 1982-04-23 1987-03-11 Sintef Fremgangsmaate for fremstilling av magnetiske polymerpartikler.
NL8900725A (nl) * 1989-03-23 1990-10-16 Az Univ Amsterdam Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur.
US5334499A (en) * 1989-04-17 1994-08-02 Eastman Kodak Company Methods of extracting, amplifying and detecting a nucleic acid from whole blood or PBMC fraction
US5200314A (en) * 1990-03-23 1993-04-06 Chiron Corporation Polynucleotide capture assay employing in vitro amplification
WO1992008133A1 (en) * 1990-10-29 1992-05-14 Dekalb Plant Genetics Isolation of biological materials using magnetic particles
US5646001A (en) * 1991-03-25 1997-07-08 Immunivest Corporation Affinity-binding separation and release of one or more selected subset of biological entities from a mixed population thereof

Also Published As

Publication number Publication date
AU8113194A (en) 1995-05-29
GB9323305D0 (en) 1994-01-05
WO1995013368A1 (en) 1995-05-18
EP0728198B1 (de) 2003-09-10
AU682074B2 (en) 1997-09-18
DE69433136T2 (de) 2004-07-08
ES2206485T3 (es) 2004-05-16
DK0728198T3 (da) 2003-10-13
EP0728198A1 (de) 1996-08-28
DE69433136D1 (de) 2003-10-16
US6090935A (en) 2000-07-18

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