ATE249516T1 - Nukleinsäure-isolierung - Google Patents

Nukleinsäure-isolierung

Info

Publication number
ATE249516T1
ATE249516T1 AT95900235T AT95900235T ATE249516T1 AT E249516 T1 ATE249516 T1 AT E249516T1 AT 95900235 T AT95900235 T AT 95900235T AT 95900235 T AT95900235 T AT 95900235T AT E249516 T1 ATE249516 T1 AT E249516T1
Authority
AT
Austria
Prior art keywords
nucleic acid
pct
sec
sample
date
Prior art date
Application number
AT95900235T
Other languages
English (en)
Inventor
Jarle Breivik
Gustav Gaudernack
Anne Spurkland
Original Assignee
Qiagen As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qiagen As filed Critical Qiagen As
Application granted granted Critical
Publication of ATE249516T1 publication Critical patent/ATE249516T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Sampling And Sample Adjustment (AREA)
AT95900235T 1993-11-11 1994-11-10 Nukleinsäure-isolierung ATE249516T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB939323305A GB9323305D0 (en) 1993-11-11 1993-11-11 Isoaltion of nucleic acid
PCT/GB1994/002469 WO1995013368A1 (en) 1993-11-11 1994-11-10 Isolation of nucleic acid

Publications (1)

Publication Number Publication Date
ATE249516T1 true ATE249516T1 (de) 2003-09-15

Family

ID=10745030

Family Applications (1)

Application Number Title Priority Date Filing Date
AT95900235T ATE249516T1 (de) 1993-11-11 1994-11-10 Nukleinsäure-isolierung

Country Status (9)

Country Link
US (1) US6090935A (de)
EP (1) EP0728198B1 (de)
AT (1) ATE249516T1 (de)
AU (1) AU682074B2 (de)
DE (1) DE69433136T2 (de)
DK (1) DK0728198T3 (de)
ES (1) ES2206485T3 (de)
GB (1) GB9323305D0 (de)
WO (1) WO1995013368A1 (de)

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GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
JP2965131B2 (ja) 1995-07-07 1999-10-18 東洋紡績株式会社 核酸結合用磁性担体およびそれを用いる核酸単離方法
AU2324997A (en) * 1996-03-15 1997-10-01 Penn State Research Foundation, The Detection of extracellular tumor-associated nucleic acid in blood plasma or ser um using nucleic acid amplification assays
US8048629B2 (en) 1996-03-15 2011-11-01 The Penn State Research Foundation Detection of extracellular tumor-associated nucleic acid in blood plasma or serum
ES2126513B1 (es) * 1997-03-14 1999-12-01 Consejo Superior Investigacion Procedimiento para la deteccion de hongos y bacterias en conductos de aire acondicionado.
US6914137B2 (en) 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
US5973138A (en) 1998-10-30 1999-10-26 Becton Dickinson And Company Method for purification and manipulation of nucleic acids using paramagnetic particles
WO2001072998A2 (de) * 2000-03-31 2001-10-04 Memorec Stoffel Gmbh Verfahren zur extraktion von nukleinsäuren
ES2319101T3 (es) * 2000-05-19 2009-05-04 Becton Dickinson And Company Sistema y procedimiento de manipulacion de particulas magneticamente sensibles en muestras de fluido para extraer el adn o el arn de una muestra.
US6672458B2 (en) * 2000-05-19 2004-01-06 Becton, Dickinson And Company System and method for manipulating magnetically responsive particles fluid samples to collect DNA or RNA from a sample
US20030087286A1 (en) * 2000-09-06 2003-05-08 Hodge Timothy A. Isolation of eukaryotic genomic DNA using magnetically responsive solid functionalized particles
US20050272085A1 (en) * 2000-09-06 2005-12-08 Hodge Timothy A Methods for forensic and congenic screening
US20050266494A1 (en) * 2000-09-06 2005-12-01 Hodge Timothy A System and method for computer network ordering of biological testing
EP1978110B1 (de) * 2000-09-06 2010-05-26 Transnetyx, Inc. Computer-basiertes Verfahren und System zum Screenen von genomischer DNS
KR20020065509A (ko) * 2000-09-06 2002-08-13 티모시 에이. 호지 유전자이식 및 표적 돌연변이유발 스크리닝을 위한시스템, 방법 및 장치
US20030082605A1 (en) * 2000-09-06 2003-05-01 Hodge Timothy A. Genomic DNA detection method and system thereof
US7494817B2 (en) * 2000-09-06 2009-02-24 Transnet Yx, Inc. Methods for genotype screening using magnetic particles
US20050239125A1 (en) * 2000-09-06 2005-10-27 Hodge Timothy A Methods for genotype screening
US20030073110A1 (en) * 2001-07-03 2003-04-17 Masaharu Aritomi Method for isolating nucleic acid and a cartridge for chemical reaction and for nucleic acid isolation
US20060014186A1 (en) * 2001-09-04 2006-01-19 Hodge Timothy A Methods for genotype screening of a strain disposed on an adsorbent carrier
GB0212826D0 (en) 2002-05-31 2002-07-10 Dna Res Innovations Ltd Materials and methods relating to polyions and substance delivery
US8048627B2 (en) * 2003-07-05 2011-11-01 The Johns Hopkins University Method and compositions for detection and enumeration of genetic variations
JP4476050B2 (ja) * 2004-06-30 2010-06-09 株式会社ニデック 視野計
US7527929B2 (en) 2004-07-30 2009-05-05 Agencourt Bioscience Corporation Methods of isolating nucleic acids using multifunctional group-coated solid phase carriers
US20090215124A1 (en) 2005-02-15 2009-08-27 Weidong Cao Nucleic acid isolation methods and materials and devices thereof
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
JP5643650B2 (ja) 2007-11-21 2014-12-17 コスモシド・インコーポレーテッド ゲノム同定システム
US8478544B2 (en) 2007-11-21 2013-07-02 Cosmosid Inc. Direct identification and measurement of relative populations of microorganisms with direct DNA sequencing and probabilistic methods
DE102008010692A1 (de) 2008-02-22 2009-08-27 Qiagen Gmbh Neue Matrices, Verfahren zu ihrer Herstellung und ihre Verwendung in Verfahren zur Isolierung von Biomolekülen
DE102008010693A1 (de) 2008-02-22 2009-08-27 Qiagen Gmbh Neue Matrixmaterialien, Verfahren zu ihrer Herstellung und ihre Verwendung in Verfahren zur Isolierung von Biomolekülen
RU2372406C1 (ru) * 2008-02-27 2009-11-10 Государственное образовательное учреждение высшего профессионального образования "Пермская государственная медицинская академия имени академика Е.А. Вагнера Федерального агентства по здравоохранению и социальному развитию" Способ выявления обсемененности объектов внешней среды грамотрицательными бактериями рода pseudomonas и acinetobacter
WO2010028382A2 (en) 2008-09-05 2010-03-11 Mayo Foundation For Medical Education And Research Collecting and processing complex macromolecular mixtures
DE102008055120A1 (de) * 2008-12-23 2010-07-01 Qiagen Gmbh Präparation und Amplifikation von Nukleinsäuren mittels magnetischer Partikel
US8574835B2 (en) * 2009-05-29 2013-11-05 Life Technologies Corporation Scaffolded nucleic acid polymer particles and methods of making and using
US8653567B2 (en) 2010-07-03 2014-02-18 Life Technologies Corporation Chemically sensitive sensor with lightly doped drains
TWI425977B (zh) 2010-11-24 2014-02-11 Ind Tech Res Inst 將福馬林固定石蠟包埋之組織進行脫蠟的方法與其套組
ES2986436T3 (es) 2011-04-15 2024-11-11 Univ Johns Hopkins Sistema de secuenciación segura
CN102229927B (zh) * 2011-06-03 2016-03-23 聂棱 提高案件微量检材脱落细胞dna提取效率的方法及试剂
WO2014035090A1 (ko) 2012-08-28 2014-03-06 주식회사 바이오큐브시스템 생물학적 시료로부터 핵산 증폭 반응용 생물학적 분자를 신속하게 분리하기 위한 다공성 고체상 및 이의 용도
ES2886507T5 (es) 2012-10-29 2024-11-15 Univ Johns Hopkins Prueba de Papanicolaou para cánceres de ovario y de endometrio
US11286531B2 (en) 2015-08-11 2022-03-29 The Johns Hopkins University Assaying ovarian cyst fluid
CN111868260B (zh) 2017-08-07 2025-02-21 约翰斯霍普金斯大学 用于评估和治疗癌症的方法和材料
ES2964100T3 (es) 2017-09-18 2024-04-04 Phase Scient International Ltd Método de utilización de un sistema acuoso bifásico para el aislamiento, purificación y/o concentración de fragmentos cortos de ácidos nucleicos
EP4103748A4 (de) 2020-02-14 2024-03-13 The Johns Hopkins University Verfahren und materialien zur beurteilung von nukleinsäuren

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AU530410B2 (en) * 1978-02-21 1983-07-14 Sintef Preparing aqueous emulsions
NO155316C (no) * 1982-04-23 1987-03-11 Sintef Fremgangsmaate for fremstilling av magnetiske polymerpartikler.
NL8900725A (nl) * 1989-03-23 1990-10-16 Az Univ Amsterdam Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur.
US5334499A (en) * 1989-04-17 1994-08-02 Eastman Kodak Company Methods of extracting, amplifying and detecting a nucleic acid from whole blood or PBMC fraction
US5200314A (en) * 1990-03-23 1993-04-06 Chiron Corporation Polynucleotide capture assay employing in vitro amplification
JPH06510363A (ja) * 1990-10-29 1994-11-17 ディカルブ　プラント　ジェネティクス 磁気性粒子を使用する生物学的材料の単離
US5646001A (en) * 1991-03-25 1997-07-08 Immunivest Corporation Affinity-binding separation and release of one or more selected subset of biological entities from a mixed population thereof

Also Published As

Publication number Publication date
AU682074B2 (en) 1997-09-18
ES2206485T3 (es) 2004-05-16
WO1995013368A1 (en) 1995-05-18
GB9323305D0 (en) 1994-01-05
DE69433136D1 (de) 2003-10-16
US6090935A (en) 2000-07-18
EP0728198A1 (de) 1996-08-28
AU8113194A (en) 1995-05-29
DK0728198T3 (da) 2003-10-13
DE69433136T2 (de) 2004-07-08
EP0728198B1 (de) 2003-09-10

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