ATE250142T1 - Methode zur sequenzierung von polynukleotiden - Google Patents

Methode zur sequenzierung von polynukleotiden

Info

Publication number
ATE250142T1
ATE250142T1 AT00910209T AT00910209T ATE250142T1 AT E250142 T1 ATE250142 T1 AT E250142T1 AT 00910209 T AT00910209 T AT 00910209T AT 00910209 T AT00910209 T AT 00910209T AT E250142 T1 ATE250142 T1 AT E250142T1
Authority
AT
Austria
Prior art keywords
target
primer
extension products
labeled
hybrid
Prior art date
Application number
AT00910209T
Other languages
English (en)
Inventor
Malcolm D Mcginnis
Alex M Madonik
Original Assignee
Applera Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applera Corp filed Critical Applera Corp
Application granted granted Critical
Publication of ATE250142T1 publication Critical patent/ATE250142T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT00910209T 1999-02-16 2000-02-16 Methode zur sequenzierung von polynukleotiden ATE250142T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/250,422 US6635419B1 (en) 1999-02-16 1999-02-16 Polynucleotide sequencing method
PCT/US2000/004034 WO2000049178A2 (en) 1999-02-16 2000-02-16 Polynucleotide sequencing method

Publications (1)

Publication Number Publication Date
ATE250142T1 true ATE250142T1 (de) 2003-10-15

Family

ID=22947677

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00910209T ATE250142T1 (de) 1999-02-16 2000-02-16 Methode zur sequenzierung von polynukleotiden

Country Status (8)

Country Link
US (2) US6635419B1 (de)
EP (1) EP1159454B1 (de)
JP (1) JP4113675B2 (de)
AT (1) ATE250142T1 (de)
AU (1) AU764099B2 (de)
CA (1) CA2362245C (de)
DE (1) DE60005309T2 (de)
WO (1) WO2000049178A2 (de)

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WO2010033200A2 (en) * 2008-09-19 2010-03-25 President And Fellows Of Harvard College Creation of libraries of droplets and related species
EP3150724A1 (de) 2008-12-19 2017-04-05 President and Fellows of Harvard College Partikelunterstützte nukleinsäuresequenzierung
JP5791621B2 (ja) 2009-10-27 2015-10-07 プレジデント アンド フェローズ オブ ハーバード カレッジ 液滴生成技術
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
AU2013302756C1 (en) 2012-08-14 2018-05-17 10X Genomics, Inc. Microcapsule compositions and methods
CA2894694C (en) 2012-12-14 2023-04-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
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US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
WO2015069634A1 (en) 2013-11-08 2015-05-14 President And Fellows Of Harvard College Microparticles, methods for their preparation and use
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
BR112016023625A2 (pt) 2014-04-10 2018-06-26 10X Genomics, Inc. dispositivos fluídicos, sistemas e métodos para encapsular e particionar reagentes, e aplicações dos mesmos
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
CA2953469A1 (en) 2014-06-26 2015-12-30 10X Genomics, Inc. Analysis of nucleic acid sequences
WO2015200893A2 (en) 2014-06-26 2015-12-30 10X Genomics, Inc. Methods of analyzing nucleic acids from individual cells or cell populations
KR20170023979A (ko) 2014-06-26 2017-03-06 10엑스 제노믹스, 인크. 핵산 서열 조립을 위한 프로세스 및 시스템
BR112017008877A2 (pt) 2014-10-29 2018-07-03 10X Genomics Inc métodos e composições para sequenciamento de ácido nucleico-alvo
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
JP6769969B2 (ja) 2015-01-12 2020-10-14 １０エックス ジェノミクス， インコーポレイテッド 核酸配列決定ライブラリーを作製するためのプロセス及びシステム、並びにこれらを使用して作製したライブラリー
WO2016115273A1 (en) 2015-01-13 2016-07-21 10X Genomics, Inc. Systems and methods for visualizing structural variation and phasing information
WO2016130578A1 (en) 2015-02-09 2016-08-18 10X Genomics, Inc. Systems and methods for determining structural variation and phasing using variant call data
EP3262407B1 (de) 2015-02-24 2023-08-30 10X Genomics, Inc. Partitionsverarbeitungsverfahren und -systeme
MX2017010857A (es) 2015-02-24 2017-12-11 10X Genomics Inc Metodos para la cobertura de secuencia de acidos nucleicos seleccionados como diana.
JP2018537414A (ja) 2015-10-13 2018-12-20 プレジデント アンド フェローズ オブ ハーバード カレッジ ゲルマイクロスフェアの作製及び使用のためのシステム及び方法
JP6954899B2 (ja) 2015-12-04 2021-10-27 １０エックス ゲノミクス，インコーポレイテッド 核酸の解析のための方法及び組成物
WO2017138984A1 (en) 2016-02-11 2017-08-17 10X Genomics, Inc. Systems, methods, and media for de novo assembly of whole genome sequence data
WO2017197343A2 (en) 2016-05-12 2017-11-16 10X Genomics, Inc. Microfluidic on-chip filters
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP4029939B1 (de) 2017-01-30 2023-06-28 10X Genomics, Inc. Verfahren und systeme für eine einzelzellbarcodierung auf tröpfchenbasis
US12264411B2 (en) 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
EP3625715A4 (de) 2017-05-19 2021-03-17 10X Genomics, Inc. Systeme und verfahren zur analyse von datensätzen
US10400235B2 (en) 2017-05-26 2019-09-03 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
EP3445876B1 (de) 2017-05-26 2023-07-05 10X Genomics, Inc. Einzelzellenanalyse von transposasezugänglichem chromatin
SG11201913654QA (en) 2017-11-15 2020-01-30 10X Genomics Inc Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
CN112262218B (zh) 2018-04-06 2024-11-08 10X基因组学有限公司 用于单细胞处理中的质量控制的系统和方法
CN115428088A (zh) 2020-02-13 2022-12-02 10X基因组学有限公司 用于基因表达和dna染色质可及性的联合交互式可视化的系统和方法
US20240344125A1 (en) * 2021-09-06 2024-10-17 Hitachi High-Tech Corporation Nucleotide Sequence Identification Method

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Also Published As

Publication number Publication date
AU764099B2 (en) 2003-08-07
US6635419B1 (en) 2003-10-21
EP1159454B1 (de) 2003-09-17
DE60005309T2 (de) 2004-06-24
CA2362245C (en) 2008-12-09
EP1159454A2 (de) 2001-12-05
WO2000049178A2 (en) 2000-08-24
DE60005309D1 (de) 2003-10-23
US20040063138A1 (en) 2004-04-01
JP2002536980A (ja) 2002-11-05
JP4113675B2 (ja) 2008-07-09
CA2362245A1 (en) 2000-08-24
WO2000049178A3 (en) 2000-12-14
AU3233900A (en) 2000-09-04

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