ATE250142T1 - Methode zur sequenzierung von polynukleotiden - Google Patents
Methode zur sequenzierung von polynukleotidenInfo
- Publication number
- ATE250142T1 ATE250142T1 AT00910209T AT00910209T ATE250142T1 AT E250142 T1 ATE250142 T1 AT E250142T1 AT 00910209 T AT00910209 T AT 00910209T AT 00910209 T AT00910209 T AT 00910209T AT E250142 T1 ATE250142 T1 AT E250142T1
- Authority
- AT
- Austria
- Prior art keywords
- target
- primer
- extension products
- labeled
- hybrid
- Prior art date
Links
- 239000002157 polynucleotide Substances 0.000 title abstract 4
- 102000040430 polynucleotide Human genes 0.000 title abstract 4
- 108091033319 polynucleotide Proteins 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 3
- 238000012163 sequencing technique Methods 0.000 title 1
- 239000002773 nucleotide Substances 0.000 abstract 4
- 125000003729 nucleotide group Chemical group 0.000 abstract 2
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 230000000295 complement effect Effects 0.000 abstract 1
- 239000011159 matrix material Substances 0.000 abstract 1
- 239000000178 monomer Substances 0.000 abstract 1
- 239000002777 nucleoside Substances 0.000 abstract 1
- 150000003833 nucleoside derivatives Chemical class 0.000 abstract 1
- 238000007873 sieving Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/250,422 US6635419B1 (en) | 1999-02-16 | 1999-02-16 | Polynucleotide sequencing method |
| PCT/US2000/004034 WO2000049178A2 (en) | 1999-02-16 | 2000-02-16 | Polynucleotide sequencing method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE250142T1 true ATE250142T1 (de) | 2003-10-15 |
Family
ID=22947677
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT00910209T ATE250142T1 (de) | 1999-02-16 | 2000-02-16 | Methode zur sequenzierung von polynukleotiden |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US6635419B1 (de) |
| EP (1) | EP1159454B1 (de) |
| JP (1) | JP4113675B2 (de) |
| AT (1) | ATE250142T1 (de) |
| AU (1) | AU764099B2 (de) |
| CA (1) | CA2362245C (de) |
| DE (1) | DE60005309T2 (de) |
| WO (1) | WO2000049178A2 (de) |
Families Citing this family (53)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6635419B1 (en) * | 1999-02-16 | 2003-10-21 | Applera Corporation | Polynucleotide sequencing method |
| WO2006085964A2 (en) | 2004-06-30 | 2006-08-17 | Applera Corporation | Log-linear amplification |
| FI20075124A0 (fi) * | 2007-02-21 | 2007-02-21 | Valtion Teknillinen | Menetelmä ja testikitti nukleotidivariaatioiden toteamiseksi |
| WO2008109176A2 (en) * | 2007-03-07 | 2008-09-12 | President And Fellows Of Harvard College | Assays and other reactions involving droplets |
| JP5738597B2 (ja) | 2007-12-21 | 2015-06-24 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 核酸の配列決定のためのシステムおよび方法 |
| US20110218123A1 (en) | 2008-09-19 | 2011-09-08 | President And Fellows Of Harvard College | Creation of libraries of droplets and related species |
| EP3290531B1 (de) | 2008-12-19 | 2019-07-24 | President and Fellows of Harvard College | Partikelunterstützte nukleinsäuresequenzierung |
| EP2493619B1 (de) | 2009-10-27 | 2018-12-19 | President and Fellows of Harvard College | Verfahren zur tröpfchenbildung |
| US9701998B2 (en) | 2012-12-14 | 2017-07-11 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| CN114891871A (zh) | 2012-08-14 | 2022-08-12 | 10X基因组学有限公司 | 微胶囊组合物及方法 |
| US10221442B2 (en) | 2012-08-14 | 2019-03-05 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| US11591637B2 (en) | 2012-08-14 | 2023-02-28 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| US10752949B2 (en) | 2012-08-14 | 2020-08-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10584381B2 (en) | 2012-08-14 | 2020-03-10 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10273541B2 (en) | 2012-08-14 | 2019-04-30 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US9951386B2 (en) | 2014-06-26 | 2018-04-24 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10323279B2 (en) | 2012-08-14 | 2019-06-18 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| EP3567116A1 (de) | 2012-12-14 | 2019-11-13 | 10X Genomics, Inc. | Verfahren und systeme zur verarbeitung von polynukleotiden |
| US10533221B2 (en) | 2012-12-14 | 2020-01-14 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| KR20230003659A (ko) | 2013-02-08 | 2023-01-06 | 10엑스 제노믹스, 인크. | 폴리뉴클레오티드 바코드 생성 |
| US10395758B2 (en) | 2013-08-30 | 2019-08-27 | 10X Genomics, Inc. | Sequencing methods |
| WO2015069634A1 (en) | 2013-11-08 | 2015-05-14 | President And Fellows Of Harvard College | Microparticles, methods for their preparation and use |
| US9824068B2 (en) | 2013-12-16 | 2017-11-21 | 10X Genomics, Inc. | Methods and apparatus for sorting data |
| US9694361B2 (en) | 2014-04-10 | 2017-07-04 | 10X Genomics, Inc. | Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same |
| KR20170026383A (ko) | 2014-06-26 | 2017-03-08 | 10엑스 제노믹스, 인크. | 핵산 서열의 분석 |
| US12312640B2 (en) | 2014-06-26 | 2025-05-27 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| EP4235677A3 (de) | 2014-06-26 | 2023-11-22 | 10X Genomics, Inc. | Verfahren und systeme zur nukleinsäuresequenzanordnung |
| EP4574974A3 (de) | 2014-06-26 | 2025-10-08 | 10x Genomics, Inc. | Verfahren zur analyse von nukleinsäuren aus einzelnen zellen oder zellpopulationen |
| JP2017532042A (ja) | 2014-10-29 | 2017-11-02 | １０エックス ゲノミクス，インコーポレイテッド | 標的化核酸配列決定のための方法及び組成物 |
| US9975122B2 (en) | 2014-11-05 | 2018-05-22 | 10X Genomics, Inc. | Instrument systems for integrated sample processing |
| AU2016207023B2 (en) | 2015-01-12 | 2019-12-05 | 10X Genomics, Inc. | Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same |
| CA2968417A1 (en) | 2015-01-13 | 2016-07-21 | 10X Genomics, Inc. | Systems and methods for visualizing structural variation and phasing information |
| SG11201705996PA (en) | 2015-02-09 | 2017-09-28 | 10X Genomics Inc | Systems and methods for determining structural variation and phasing using variant call data |
| EP4286516A3 (de) | 2015-02-24 | 2024-03-06 | 10X Genomics, Inc. | Partitionsverarbeitungsverfahren und -systeme |
| AU2016222719B2 (en) | 2015-02-24 | 2022-03-31 | 10X Genomics, Inc. | Methods for targeted nucleic acid sequence coverage |
| US11123297B2 (en) | 2015-10-13 | 2021-09-21 | President And Fellows Of Harvard College | Systems and methods for making and using gel microspheres |
| KR102723865B1 (ko) | 2015-12-04 | 2024-10-29 | 10엑스 제노믹스, 인크. | 핵산 분석을 위한 방법 및 조성물 |
| JP6735348B2 (ja) | 2016-02-11 | 2020-08-05 | １０エックス ジェノミクス， インコーポレイテッド | 全ゲノム配列データのデノボアセンブリのためのシステム、方法及び媒体 |
| WO2017197343A2 (en) | 2016-05-12 | 2017-11-16 | 10X Genomics, Inc. | Microfluidic on-chip filters |
| WO2017197338A1 (en) | 2016-05-13 | 2017-11-16 | 10X Genomics, Inc. | Microfluidic systems and methods of use |
| US10815525B2 (en) | 2016-12-22 | 2020-10-27 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10011872B1 (en) | 2016-12-22 | 2018-07-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10550429B2 (en) | 2016-12-22 | 2020-02-04 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| EP4310183B1 (de) | 2017-01-30 | 2025-07-09 | 10X Genomics, Inc. | Verfahren und systeme zur tröpfchenbasierten einzelzellenbarcodierung |
| US12264411B2 (en) | 2017-01-30 | 2025-04-01 | 10X Genomics, Inc. | Methods and systems for analysis |
| WO2018213774A1 (en) | 2017-05-19 | 2018-11-22 | 10X Genomics, Inc. | Systems and methods for analyzing datasets |
| US10844372B2 (en) | 2017-05-26 | 2020-11-24 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
| CN116064732A (zh) | 2017-05-26 | 2023-05-05 | 10X基因组学有限公司 | 转座酶可接近性染色质的单细胞分析 |
| EP3625361A1 (de) | 2017-11-15 | 2020-03-25 | 10X Genomics, Inc. | Funktionalisierte gelperlen |
| WO2019099908A1 (en) | 2017-11-17 | 2019-05-23 | 10X Genomics, Inc. | Methods and systems for associating physical and genetic properties of biological particles |
| EP3775271B1 (de) | 2018-04-06 | 2025-03-12 | 10X Genomics, Inc. | Systeme und verfahren zur qualitätskontrolle in einer einzelzellenverarbeitung |
| EP4104179B1 (de) | 2020-02-13 | 2026-01-28 | 10X Genomics, Inc. | Systeme und methoden zur gemeinsamen interaktiven visualisierung der genexpression und der zugänglichkeit von dna-chromatin |
| CN117795097A (zh) * | 2021-09-06 | 2024-03-29 | 株式会社日立高新技术 | 核苷酸序列确定方法 |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA1340806C (en) | 1986-07-02 | 1999-11-02 | James Merrill Prober | Method, system and reagents for dna sequencing |
| US5366860A (en) | 1989-09-29 | 1994-11-22 | Applied Biosystems, Inc. | Spectrally resolvable rhodamine dyes for nucleic acid sequence determination |
| US5654442A (en) | 1989-11-14 | 1997-08-05 | The Perkin-Elmer Corporation | 4,7-dichlorofluorescein dyes as molecular probes |
| WO1991011533A1 (en) * | 1990-01-26 | 1991-08-08 | E.I. Du Pont De Nemours And Company | Method for isolating primer extension products from template-directed dna polymerase reactions |
| US5187085A (en) | 1990-09-28 | 1993-02-16 | Applied Biosystems, Inc. | Nucleic acid sequence analysis with nucleoside-5'-o-(1-thiotriphosphates) |
| US5210412A (en) * | 1991-01-31 | 1993-05-11 | Wayne State University | Method for analyzing an organic sample |
| US5470705A (en) | 1992-04-03 | 1995-11-28 | Applied Biosystems, Inc. | Probe composition containing a binding domain and polymer chain and methods of use |
| JP2775346B2 (ja) | 1992-04-03 | 1998-07-16 | アプライド バイオシステムズ，インコーポレイテッド | プローブ構成物および方法 |
| WO1995024505A1 (en) | 1994-03-08 | 1995-09-14 | Amersham International Plc | Modifying nucleotide analogues |
| US5976798A (en) | 1994-03-30 | 1999-11-02 | Mitokor | Methods for detecting mitochondrial mutations diagnostic for Alzheimer's disease and methods for determining heteroplasmy of mitochondrial nucleic acid |
| US5800996A (en) | 1996-05-03 | 1998-09-01 | The Perkin Elmer Corporation | Energy transfer dyes with enchanced fluorescence |
| US5656145A (en) * | 1996-05-20 | 1997-08-12 | Bio-Rad Laboratories, Inc. | Needle guide for loading samples into a vertical slab gel |
| US6635419B1 (en) * | 1999-02-16 | 2003-10-21 | Applera Corporation | Polynucleotide sequencing method |
-
1999
- 1999-02-16 US US09/250,422 patent/US6635419B1/en not_active Expired - Lifetime
-
2000
- 2000-02-16 EP EP00910209A patent/EP1159454B1/de not_active Expired - Lifetime
- 2000-02-16 JP JP2000599901A patent/JP4113675B2/ja not_active Expired - Fee Related
- 2000-02-16 DE DE60005309T patent/DE60005309T2/de not_active Expired - Lifetime
- 2000-02-16 WO PCT/US2000/004034 patent/WO2000049178A2/en not_active Ceased
- 2000-02-16 AU AU32339/00A patent/AU764099B2/en not_active Ceased
- 2000-02-16 AT AT00910209T patent/ATE250142T1/de not_active IP Right Cessation
- 2000-02-16 CA CA002362245A patent/CA2362245C/en not_active Expired - Fee Related
-
2003
- 2003-10-10 US US10/684,199 patent/US20040063138A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US6635419B1 (en) | 2003-10-21 |
| WO2000049178A2 (en) | 2000-08-24 |
| EP1159454B1 (de) | 2003-09-17 |
| DE60005309T2 (de) | 2004-06-24 |
| EP1159454A2 (de) | 2001-12-05 |
| CA2362245A1 (en) | 2000-08-24 |
| DE60005309D1 (de) | 2003-10-23 |
| CA2362245C (en) | 2008-12-09 |
| AU764099B2 (en) | 2003-08-07 |
| AU3233900A (en) | 2000-09-04 |
| JP2002536980A (ja) | 2002-11-05 |
| WO2000049178A3 (en) | 2000-12-14 |
| US20040063138A1 (en) | 2004-04-01 |
| JP4113675B2 (ja) | 2008-07-09 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |