ATE256191T1 - Ein pilz, in dem das area-gen verändert ist, und ein area-gen aus aspergillus oryzae - Google Patents

Ein pilz, in dem das area-gen verändert ist, und ein area-gen aus aspergillus oryzae

Info

Publication number
ATE256191T1
ATE256191T1 AT95922443T AT95922443T ATE256191T1 AT E256191 T1 ATE256191 T1 AT E256191T1 AT 95922443 T AT95922443 T AT 95922443T AT 95922443 T AT95922443 T AT 95922443T AT E256191 T1 ATE256191 T1 AT E256191T1
Authority
AT
Austria
Prior art keywords
area gene
pct
aspergillus oryzae
modified
mushroom
Prior art date
Application number
AT95922443T
Other languages
English (en)
Inventor
Tove Christensen
Michael J Hynes
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of ATE256191T1 publication Critical patent/ATE256191T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/62—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from Aspergillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6427—Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines Containing Plant Substances (AREA)
AT95922443T 1994-06-17 1995-06-19 Ein pilz, in dem das area-gen verändert ist, und ein area-gen aus aspergillus oryzae ATE256191T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK71794 1994-06-17
PCT/DK1995/000254 WO1995035385A1 (en) 1994-06-17 1995-06-19 A FUNGUS WHEREIN THE areA GENE HAS BEEN MODIFIED AND AN areA GENE FROM ASPERGILLUS ORYZAE

Publications (1)

Publication Number Publication Date
ATE256191T1 true ATE256191T1 (de) 2003-12-15

Family

ID=8096735

Family Applications (1)

Application Number Title Priority Date Filing Date
AT95922443T ATE256191T1 (de) 1994-06-17 1995-06-19 Ein pilz, in dem das area-gen verändert ist, und ein area-gen aus aspergillus oryzae

Country Status (10)

Country Link
US (1) US6025185A (de)
EP (1) EP0770139B1 (de)
JP (1) JPH10501414A (de)
CN (1) CN1253574C (de)
AT (1) ATE256191T1 (de)
AU (1) AU2733895A (de)
DE (1) DE69532283T2 (de)
DK (1) DK0770139T3 (de)
FI (1) FI965031L (de)
WO (1) WO1995035385A1 (de)

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AU1092897A (en) * 1995-12-15 1997-07-14 Novo Nordisk A/S A funguns wherein the area, pepc and/or pepe genes have been inactivated
CN100432099C (zh) 1996-07-05 2008-11-12 诺沃奇梅兹有限公司 转录因子
US5811381A (en) * 1996-10-10 1998-09-22 Mark A. Emalfarb Cellulase compositions and methods of use
US7883872B2 (en) * 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
DE69729085T2 (de) * 1997-07-05 2005-04-21 Nestle Sa Gesteigerte Expression proteolytischer Enzyme in ...
DK1117798T3 (da) * 1998-10-05 2007-04-10 Novozymes As Fungal transskriptionel aktivator egnet ved fremgangsmåder til produktion af polypeptider
US6806062B1 (en) 1998-10-05 2004-10-19 Novozymes A/S Fungal transcriptional activator useful in methods for producing polypeptides
KR100618495B1 (ko) * 1998-10-06 2006-08-31 마크 아론 에말파브 섬유상의 진균성 숙주 영역에서의 형질전환 시스템:크리소스포륨속에서
CN1197965C (zh) 1998-10-26 2005-04-20 诺维信公司 在丝状真菌细胞内构建和筛选目的dna文库
DE60142226D1 (de) * 2000-03-14 2010-07-08 Novozymes As Pilz transkriptionsaktivator zur verwendung in verfahren zur herstellung von polypeptiden
US9862956B2 (en) 2006-12-10 2018-01-09 Danisco Us Inc. Expression and high-throughput screening of complex expressed DNA libraries in filamentous fungi
EP2505651A3 (de) 2006-12-10 2013-01-09 Dyadic International, Inc. Pilzisolate mit reduzierter Proteaseaktivität
US8551751B2 (en) * 2007-09-07 2013-10-08 Dyadic International, Inc. BX11 enzymes having xylosidase activity
EP2438163B1 (de) 2009-06-02 2015-01-21 Novozymes Inc. Polypeptide mit cellobiohydrolase-aktivität und dafür codierende polynukleotide
US8143021B2 (en) 2009-07-07 2012-03-27 Novozymes, Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
DK2483295T3 (en) 2009-09-29 2016-02-22 Novozymes Inc Polypeptides having cellulolytic enhancing activity and polynucleotides encoding them
CA2775347A1 (en) 2009-09-29 2011-04-07 Novozymes A/S Polypeptides having xylanase activity and polynucleotides encoding same
CN102695720B (zh) 2009-09-30 2018-02-16 诺维信股份有限公司 具有纤维素分解增强活性的多肽和编码该多肽的多核苷酸
EP2496694B1 (de) 2009-11-06 2017-04-19 Novozymes, Inc. Zusammensetzungen zur verzuckerung von cellulosematerial
EP2635594B1 (de) 2010-11-04 2017-01-11 Novozymes Inc. Polypeptide mit cellobiohydrolaseaktivität und dafür kodierende polynukleotide
BR112013009026A2 (pt) 2010-11-12 2016-07-12 Novozymes Inc polipeptídeo isolado, célula hospedeira recombinante, métodos para produzir um polipeptídeo, um mutante de uma célula precursora, uma proteína, e um produto de fermentação, para inibir a expressão de um polipeptídeo, para degradar ou converter um material celulósico, e método para fermentar um material celulósico, planta transgênica, parte de planta ou célula de planta, molécula de rna inibidora de filamento duplo, polinucleotídeo isolado, e, formulação de caldo integral ou composição de cultura de célula
CN103384678B (zh) 2011-02-23 2017-01-18 诺维信股份有限公司 具有纤维素水解增强活性的多肽及其编码多核苷酸
US9879294B2 (en) 2011-03-25 2018-01-30 Novozymes A/S Methods for degrading or converting cellulosic material
DK2702162T3 (da) 2011-04-29 2020-05-18 Novozymes Inc Fremgangsmåder til forbedring af nedbrydningen eller omdannelsen af celluloseholdigt materiale
WO2013064075A1 (en) 2011-10-31 2013-05-10 Novozymes, Inc. Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
WO2013075644A1 (en) 2011-11-22 2013-05-30 Novozymes, Inc. Polypeptides having beta-xylosidase activity and polynucleotides encoding same
US9624481B2 (en) 2011-12-01 2017-04-18 Novozymes, Inc. Polypeptides having beta-xylosidase activity and polynucleotides encoding same
EP3272862A1 (de) 2011-12-16 2018-01-24 Novozymes, Inc. Polypeptide mit laccaseaktivität und dafür kodierende polynukleotide
BR112014014697A2 (pt) 2011-12-19 2020-10-27 Novozymes, Inc. polipeptídeo isolado, composição, polinucleotídeo isolado, construto de ácido nucleico ou vetor de expressão, célula hospedeira recombinante, métodos para produzir um polipeptídeo e uma proteína, para gerar oxigênio molecular, e para remover peróxido de hidrogênio do tecido, processos para degradar ou converter um material celulósico, e para produzir um produto de fermentação, e, formulação de caldo integral ou composição de cultura de células
RU2645252C2 (ru) 2012-01-05 2018-02-19 Глюкос Финланд Ой Клетка нитчатых грибов с дефицитом протеаз и способы ее применения
EP2852610B1 (de) 2012-05-23 2018-07-11 Glykos Finland Oy Herstellung von fucosylierten glykoproteinen
BR112015014624A2 (pt) 2012-12-24 2017-10-10 Novozymes As polipeptídeo isolado, composição, polinucleotídeo isolado, construto de ácido nucleico ou vetor de expressão, célula hospedeira recombinante, e, método para tratamento de têxtil
SG11201600115SA (en) 2013-07-10 2016-02-26 Novartis Ag Multiple proteases deficient filamentous fungal cells and methods of use thereof
US10619144B2 (en) 2013-08-23 2020-04-14 Novozymes A/S Regulated PepC expression
SG11201700446XA (en) 2014-07-21 2017-02-27 Glykos Finland Oy Production of glycoproteins with mammalian-like n-glycans in filamentous fungi

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5190931A (en) * 1983-10-20 1993-03-02 The Research Foundation Of State University Of New York Regulation of gene expression by employing translational inhibition of MRNA utilizing interfering complementary MRNA
US4885249A (en) * 1984-12-05 1989-12-05 Allelix, Inc. Aspergillus niger transformation system
JPS6236183A (ja) * 1985-06-20 1987-02-17 ザ・サルク・インステイチユ−ト・バイオテクノロジ−／インダストリアル・アソシエイツ・インコ−ポレ−テツド サツカロミセス・セレビシエからのポリペプチドの発現および分泌
DK122686D0 (da) * 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
ES2204884T3 (es) * 1988-01-05 2004-05-01 Roche Diagnostics Gmbh Procedimiento para la obtencion de proteinas o productos genicos que contienen proteinas.
CA1333777C (en) * 1988-07-01 1995-01-03 Randy M. Berka Aspartic proteinase deficient filamentous fungi
ATE220105T1 (de) * 1991-04-01 2002-07-15 Merck & Co Inc Gene, die die proteolytische aktivitaet von pichia beeinflussen und deren verwendung

Also Published As

Publication number Publication date
US6025185A (en) 2000-02-15
DE69532283D1 (de) 2004-01-22
EP0770139A1 (de) 1997-05-02
FI965031A7 (fi) 1996-12-16
AU2733895A (en) 1996-01-15
WO1995035385A1 (en) 1995-12-28
CN1253574C (zh) 2006-04-26
DE69532283T2 (de) 2004-09-23
CN1150824A (zh) 1997-05-28
FI965031A0 (fi) 1996-12-16
DK0770139T3 (da) 2004-04-13
JPH10501414A (ja) 1998-02-10
EP0770139B1 (de) 2003-12-10
FI965031L (fi) 1996-12-16

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