ATE26119T1 - Dna fragmente markiert wenigstens an einem ende mit modifizierten ribonukleotiden,die erkennbar sind durch geeignete molekuele und ein verfahren,um eine analyse solcher dna fragmente auszufuehren. - Google Patents

Dna fragmente markiert wenigstens an einem ende mit modifizierten ribonukleotiden,die erkennbar sind durch geeignete molekuele und ein verfahren,um eine analyse solcher dna fragmente auszufuehren.

Info

Publication number
ATE26119T1
ATE26119T1 AT83900050T AT83900050T ATE26119T1 AT E26119 T1 ATE26119 T1 AT E26119T1 AT 83900050 T AT83900050 T AT 83900050T AT 83900050 T AT83900050 T AT 83900050T AT E26119 T1 ATE26119 T1 AT E26119T1
Authority
AT
Austria
Prior art keywords
dna fragments
dna
carrying
ribonucleotids
recognizable
Prior art date
Application number
AT83900050T
Other languages
English (en)
Inventor
Philippe Kourilsky
Christian Vincent
Paul Tchen
Original Assignee
Pasteur Institut
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pasteur Institut filed Critical Pasteur Institut
Application granted granted Critical
Publication of ATE26119T1 publication Critical patent/ATE26119T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT83900050T 1981-12-29 1982-12-29 Dna fragmente markiert wenigstens an einem ende mit modifizierten ribonukleotiden,die erkennbar sind durch geeignete molekuele und ein verfahren,um eine analyse solcher dna fragmente auszufuehren. ATE26119T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR8124443A FR2519005B1 (fr) 1981-12-29 1981-12-29 Fragments d'adn marques a l'une au moins de leurs extremites par des ribonucleotides modifies reconnaissables par des molecules affines et procede pour realiser une analyse de tels fragments d'adn
EP83900050A EP0097667B1 (de) 1981-12-29 1982-12-29 Dna fragmente markiert wenigstens an einem ende mit modifizierten ribonukleotiden,die erkennbar sind durch geeignete moleküle und ein verfahren,um eine analyse solcher dna fragmente auszuführen
PCT/FR1982/000223 WO1983002277A1 (fr) 1981-12-29 1982-12-29 Fragment d'adn marques a l'une au moins de leurs extremites par des ribonucleotides modifies reconnaissables par des molecules affines et procede pour realiser une analyse de tels fragment d'adn

Publications (1)

Publication Number Publication Date
ATE26119T1 true ATE26119T1 (de) 1987-04-15

Family

ID=9265491

Family Applications (1)

Application Number Title Priority Date Filing Date
AT83900050T ATE26119T1 (de) 1981-12-29 1982-12-29 Dna fragmente markiert wenigstens an einem ende mit modifizierten ribonukleotiden,die erkennbar sind durch geeignete molekuele und ein verfahren,um eine analyse solcher dna fragmente auszufuehren.

Country Status (8)

Country Link
EP (1) EP0097667B1 (de)
JP (1) JPS58502205A (de)
AT (1) ATE26119T1 (de)
CA (1) CA1201670A (de)
DE (1) DE3275833D1 (de)
FR (1) FR2519005B1 (de)
IT (1) IT1155089B (de)
WO (1) WO1983002277A1 (de)

Families Citing this family (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1219824A (en) * 1981-04-17 1987-03-31 David C. Ward Modified nucleotides and methods of preparing and using same
US4711955A (en) * 1981-04-17 1987-12-08 Yale University Modified nucleotides and methods of preparing and using same
CA1223831A (en) 1982-06-23 1987-07-07 Dean Engelhardt Modified nucleotides, methods of preparing and utilizing and compositions containing the same
US7220854B1 (en) 1982-06-23 2007-05-22 Enzo Life Sciences, Inc. C/O Enzo Biochem, Inc. Sugar moiety labeled nucleotide, and an oligo- or polynucleotide, and other compositions comprising such sugar moiety labeled nucleotides
CA1309672C (en) * 1983-01-27 1992-11-03 Jannis G. Stavrianopoulos Methods and structures employing non-radioactive chemically-labeled polynucleotide probes
US4994373A (en) 1983-01-27 1991-02-19 Enzo Biochem, Inc. Method and structures employing chemically-labelled polynucleotide probes
CA1254525A (en) * 1983-04-13 1989-05-23 Christine L. Brakel Kit for terminally chemically labeling dna
ES8600404A1 (es) * 1983-05-02 1985-10-01 Enzo Biochem Inc Un metodo para detectar una secuencia seleccionada de acido nucleico en un material biologico.
US4626501A (en) * 1983-09-02 1986-12-02 Integrated Genetics, Inc. Labeled DNA
SE466208B (sv) * 1983-12-20 1992-01-13 California Inst Of Techn Enkelstraengad aminoderivat av oligonukleotider och foerfarande foer framstaellning daerav
US5821058A (en) * 1984-01-16 1998-10-13 California Institute Of Technology Automated DNA sequencing technique
CA1258611A (en) * 1984-01-16 1989-08-22 Lloyd M. Smith Method of dna sequencing
USRE43096E1 (en) 1984-01-16 2012-01-10 California Institute Of Technology Tagged extendable primers and extension products
US4699876A (en) * 1984-01-27 1987-10-13 E. I. Du Pont De Nemours And Company Nonradiometric polynucleotide probes
US4707440A (en) * 1984-01-30 1987-11-17 Enzo Biochem, Inc. Nucleic acid hybridization assay and detectable molecules useful in such assay
US4748111A (en) * 1984-03-12 1988-05-31 Molecular Diagnostics, Inc. Nucleic acid-protein conjugate used in immunoassay
NZ211453A (en) * 1984-03-22 1989-01-06 Biotechnology Research Enterpr Aryl azides, their preparation and use in the detection, localisation and isolation of polynucleotides
US6004446A (en) * 1984-03-29 1999-12-21 Li-Cor, Inc. DNA Sequencing
US6207421B1 (en) 1984-03-29 2001-03-27 Li-Cor, Inc. DNA sequencing and DNA terminators
US5360523A (en) * 1984-03-29 1994-11-01 Li-Cor, Inc. DNA sequencing
US5571388A (en) * 1984-03-29 1996-11-05 Li-Cor, Inc. Sequencing near infrared and infrared fluorescense labeled DNA for detecting using laser diodes and suitable labels thereof
US4729947A (en) * 1984-03-29 1988-03-08 The Board Of Regents Of The University Of Nebraska DNA sequencing
US4766062A (en) * 1984-05-07 1988-08-23 Allied Corporation Displacement polynucleotide assay method and polynucleotide complex reagent therefor
US4683194A (en) * 1984-05-29 1987-07-28 Cetus Corporation Method for detection of polymorphic restriction sites and nucleic acid sequences
DE3431536A1 (de) * 1984-08-28 1986-03-13 Boehringer Mannheim Gmbh, 6800 Mannheim Derivatisierte nucleinsaeure-sequenz, verfahren zu deren herstellung sowie deren verwendung zum nachweis von nucleinsaeuren
US5367066A (en) * 1984-10-16 1994-11-22 Chiron Corporation Oligonucleotides with selectably cleavable and/or abasic sites
US5430136A (en) * 1984-10-16 1995-07-04 Chiron Corporation Oligonucleotides having selectably cleavable and/or abasic sites
US4670379A (en) * 1984-12-19 1987-06-02 E. I. Du Pont De Nemours And Company Polynucleotide hydridization assays employing catalyzed luminescence
US4818681A (en) * 1985-02-22 1989-04-04 Molecular Diagnostics, Inc. Fast and specific immobilization of nucleic acids to solid supports
DE3689223T2 (de) * 1985-05-15 1994-03-03 Amoco Corp Cytidinanaloga.
US4855225A (en) * 1986-02-07 1989-08-08 Applied Biosystems, Inc. Method of detecting electrophoretically separated oligonucleotides
FR2601956B1 (fr) * 1986-07-22 1989-11-03 Pasteur Institut Nouveaux derives de desoxy-2' adenosine, leur procede d'obtention par voie de synthese et leurs applications biologiques
US5109124A (en) * 1988-06-01 1992-04-28 Biogen, Inc. Nucleic acid probe linked to a label having a terminal cysteine
ATE133714T1 (de) 1988-09-29 1996-02-15 Chiron Corp Polynukleotid-bestimmung mit selektierbaren spaltstellen
US5324829A (en) * 1988-12-16 1994-06-28 Ortho Diagnostic Systems, Inc. High specific activity nucleic acid probes having target recognition and signal generating moieties
DE59209203D1 (de) * 1991-08-28 1998-03-26 Boehringer Mannheim Gmbh Primer für matrizenabhängige enzymatische nukleinsäuresynthesen
EP1000177B1 (de) 1997-08-01 2007-04-25 Genetic Systems Corporation Synthetische antigene zum nachweis von antikörpern mit immunreaktivität gegen hiv-virus

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IN142734B (de) * 1975-04-28 1977-08-20 Miles Lab
US4255566A (en) * 1980-02-19 1981-03-10 Miles Laboratories Flavin adenine dinucleotide derivatives and labeled conjugates prepared therefrom

Also Published As

Publication number Publication date
IT8225021A1 (it) 1984-06-29
IT8225021A0 (it) 1982-12-29
DE3275833D1 (en) 1987-04-30
FR2519005A1 (fr) 1983-07-01
JPS58502205A (ja) 1983-12-22
CA1201670A (fr) 1986-03-11
FR2519005B1 (fr) 1985-10-25
IT1155089B (it) 1987-01-21
WO1983002277A1 (fr) 1983-07-07
EP0097667B1 (de) 1987-03-25
EP0097667A1 (de) 1984-01-11

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