ATE263245T1 - Verbesserte mutanten von (2,5 dkg) reduktase a - Google Patents

Verbesserte mutanten von (2,5 dkg) reduktase a

Info

Publication number: ATE263245T1
Authority: AT; Austria
Prior art keywords: reductase; mutants; substrate; dkg; increased
Prior art date: 1996-01-11

Application number

AT97902830T

Other languages

English (en)

Inventor

David B Powers

Stephen Anderson

Original Assignee

Univ Rutgers

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1996-01-11

Filing date

1997-01-09

Publication date

2004-04-15

1997-01-09 Application filed by Univ Rutgers filed Critical Univ Rutgers

2004-04-15 Application granted granted Critical

2004-04-15 Publication of ATE263245T1 publication Critical patent/ATE263245T1/de

Links

CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 abstract 4
239000000758 substrate Substances 0.000 abstract 3
108090000790 Enzymes Proteins 0.000 abstract 2
102000004190 Enzymes Human genes 0.000 abstract 2
101710204690 2,5-diketo-D-gluconic acid reductase A Proteins 0.000 abstract 1
VBUYCZFBVCCYFD-YVZJFKFKSA-N 2-dehydro-L-gluconic acid Chemical compound OC[C@H](O)[C@H](O)[C@@H](O)C(=O)C(O)=O VBUYCZFBVCCYFD-YVZJFKFKSA-N 0.000 abstract 1
ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 abstract 1
229930003268 Vitamin C Natural products 0.000 abstract 1
235000010323 ascorbic acid Nutrition 0.000 abstract 1
239000011668 ascorbic acid Substances 0.000 abstract 1
229960005070 ascorbic acid Drugs 0.000 abstract 1
230000005764 inhibitory process Effects 0.000 abstract 1
239000002243 precursor Substances 0.000 abstract 1
238000002741 site-directed mutagenesis Methods 0.000 abstract 1
230000007306 turnover Effects 0.000 abstract 1
235000019154 vitamin C Nutrition 0.000 abstract 1
239000011718 vitamin C Substances 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/822—Microorganisms using bacteria or actinomycetales
- Y10S435/843—Corynebacterium

Landscapes

Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Organic Chemistry (AREA)
Zoology (AREA)
Engineering & Computer Science (AREA)
Bioinformatics & Cheminformatics (AREA)
Wood Science & Technology (AREA)
Microbiology (AREA)
Medicinal Chemistry (AREA)
Biomedical Technology (AREA)
Molecular Biology (AREA)
Biochemistry (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Biotechnology (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Enzymes And Modification Thereof (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Peptides Or Proteins (AREA)
Medicines Containing Material From Animals Or Micro-Organisms (AREA)

AT97902830T 1996-01-11 1997-01-09 Verbesserte mutanten von (2,5 dkg) reduktase a ATE263245T1 (de)

Applications Claiming Priority (3)

Application Number	Priority Date	Filing Date	Title
US58401996A	1996-01-11	1996-01-11
US08/585,595 US5795761A (en)	1996-01-11	1996-01-16	Mutants of 2,5-diketo-D-gluconic acid (2,5-DKG) reductase A
PCT/US1997/000097 WO1997025432A2 (en)	1996-01-11	1997-01-09	Improved mutants of (2,5 dkg) reductase

Publications (1)

Publication Number	Publication Date
ATE263245T1 true ATE263245T1 (de)	2004-04-15

Family

ID=27078962

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AT97902830T ATE263245T1 (de)	1996-01-11	1997-01-09	Verbesserte mutanten von (2,5 dkg) reduktase a

Country Status (12)

Country	Link
US (1)	US5795761A (de)
EP (1)	EP0873411B1 (de)
JP (1)	JP3880624B2 (de)
AT (1)	ATE263245T1 (de)
AU (1)	AU719516B2 (de)
CA (1)	CA2242965A1 (de)
DE (1)	DE69728377T2 (de)
DK (1)	DK0873411T3 (de)
ES (1)	ES2218659T3 (de)
NZ (1)	NZ331000A (de)
PT (1)	PT873411E (de)
WO (1)	WO1997025432A2 (de)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US6946280B1 (en) *	1996-03-29	2005-09-20	Genencor International, Inc.	Enzyme multimer and process of producing same
US5919685A (en) *	1997-08-08	1999-07-06	Incyte Pharmaceuticals, Inc.	Human aflatoxin B1 aldehyde reductase
CN1119414C (zh) *	1997-12-30	2003-08-27	中国科学院上海生物工程研究中心	突变的2,5二酮基－d－葡萄糖酸还原酶及其基因工程表达
US6599722B2 (en) *	1998-12-22	2003-07-29	Genencor International, Inc.	Method for producing ascorbic acid intermediates
US7256027B1 (en) *	1999-06-15	2007-08-14	Rutgers, The State University Of New Jersey	Enzymes for the production of 2-keto-L-gulonic acid
JP2003522535A (ja) *	2000-02-08	2003-07-29	アバンテイス・フアルマ・エス・アー	アデノウイルスの検出及び定量方法
US6720168B2 (en)	2000-08-04	2004-04-13	Genencor International, Inc.	2,5-DKG permeases
DK1305421T3 (da) *	2000-08-04	2006-07-10	Genencor Int	Foröget 2-keto-L-gulonsyre-produktion
US6423518B1 (en)	2000-09-11	2002-07-23	Rutgers, The State University	Design and production of mutant 2,5-diketo-D-gluconic acid reductase enzymes with altered cofactor dependency
US6576452B1 (en) *	2000-10-04	2003-06-10	Genencor International, Inc.	2,5-diketo-L-gluconic acid reductases and methods of use
CN1298855C (zh)	2001-04-04	2007-02-07	金克克国际有限公司	在宿主细胞中生成产物的方法
ATE421577T1 (de) *	2001-04-04	2009-02-15	Genencor Int	Entkoppelte anabole und katabole stoffwechselwege in wirtszellen
US7229811B2 (en)	2001-08-03	2007-06-12	Genencor International, Inc.	2,5-diketo-D-gluconic acid (2,5-DKG) permeases
US7419795B2 (en) *	2003-05-22	2008-09-02	Danisco A/S, Genencor Division	Metabolically engineered bacterial strains having non-functional endogenous gluconate transporters
WO2005012486A2 (en)	2003-07-30	2005-02-10	Genencor International, Inc.	Metabolically engineered bacterial strains having enhanced 2-keto-d-gluconate accumulation
DK1649041T3 (da) *	2003-07-30	2012-01-02	Genencor Int	Multimere oxidoreduktaser
WO2011012702A1 (en) *	2009-07-30	2011-02-03	Metabolic Explorer	Mutant glycerol dehydrogenase (glydh) for the production of a biochemical by fermentation
CN113801872A (zh) *	2021-10-11	2021-12-17	杭州师范大学	一种精准调控组装制备稳定酶蛋白环的方法

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JPS5021559B2 (de) *	1973-03-22	1975-07-23
JPS5135487A (en) *	1974-09-20	1976-03-25	Shionogi Seiyaku Kk	22 keto ll guronsan no seizohoho
JPS5135485A (en) *	1974-09-20	1976-03-25	Shionogi Seiyaku Kk	22 keto ll guronsan no seizohoho
JPS6041596B2 (ja) *	1980-08-14	1985-09-18	塩野義製薬株式会社	２，５−ジケト−ｄ−グルコン酸の製造方法
JPS58162298A (ja) *	1982-03-05	1983-09-26	Shionogi & Co Ltd	２−ケト−ｌ−グロン酸の製造方法
US4543331A (en) *	1982-03-05	1985-09-24	Shionogi & Co., Ltd.	Fermentative or enzymatic production of 2-keto-L-gulonic acid
US4760025A (en) *	1984-05-29	1988-07-26	Genencor, Inc.	Modified enzymes and methods for making same
US4758514A (en) *	1983-06-28	1988-07-19	Genentech, Inc.	Ascorbic acid intermediates and process enzymes
US4757012A (en) *	1983-06-28	1988-07-12	Genentech, Inc.	Ascorbic acid intermediates and process enzymes
US5004690A (en) *	1983-06-28	1991-04-02	Genetech, Inc.	Ascorbic acid intermediates and process enzymes
JPS60118186A (ja) *	1983-11-17	1985-06-25	Shionogi & Co Ltd	２・５−ジケト−ｄ−グルコン酸リダクタ−ゼ
US5008193A (en) *	1984-06-14	1991-04-16	Genentech, Inc.	Ascorbic acid intermediates and process enzymes
GB8519536D0 (en) *	1985-08-02	1985-09-11	Biogen Nv	Vitamin c precursor
JPH0817706B2 (ja) *	1985-08-02	1996-02-28	バイオジェンインコーポレイテッド	遺伝子的に修飾された生物体を用いるビタミンｃの製造方法
US5017691A (en) *	1986-07-03	1991-05-21	Schering Corporation	Mammalian interleukin-4
US4908773A (en) *	1987-04-06	1990-03-13	Genex Corporation	Computer designed stabilized proteins and method for producing same
US4704692A (en) *	1986-09-02	1987-11-03	Ladner Robert C	Computer based system and method for determining and displaying possible chemical structures for converting double- or multiple-chain polypeptides to single-chain polypeptides
US4853871A (en) *	1987-04-06	1989-08-01	Genex Corporation	Computer-based method for designing stablized proteins
US5032514A (en) *	1988-08-08	1991-07-16	Genentech, Inc.	Metabolic pathway engineering to increase production of ascorbic acid intermediates
US5376544A (en) *	1992-09-08	1994-12-27	Rutgers The State University Of New Jersey	Enzymes for the production of 2-keto-L-gulonic acid

1996
- 1996-01-16 US US08/585,595 patent/US5795761A/en not_active Expired - Lifetime
1997
- 1997-01-09 ES ES97902830T patent/ES2218659T3/es not_active Expired - Lifetime
- 1997-01-09 CA CA002242965A patent/CA2242965A1/en not_active Abandoned
- 1997-01-09 DK DK97902830T patent/DK0873411T3/da active
- 1997-01-09 WO PCT/US1997/000097 patent/WO1997025432A2/en not_active Ceased
- 1997-01-09 NZ NZ331000A patent/NZ331000A/xx not_active IP Right Cessation
- 1997-01-09 AT AT97902830T patent/ATE263245T1/de not_active IP Right Cessation
- 1997-01-09 AU AU16911/97A patent/AU719516B2/en not_active Ceased
- 1997-01-09 JP JP52530397A patent/JP3880624B2/ja not_active Expired - Lifetime
- 1997-01-09 DE DE69728377T patent/DE69728377T2/de not_active Expired - Fee Related
- 1997-01-09 EP EP97902830A patent/EP0873411B1/de not_active Expired - Lifetime
- 1997-01-09 PT PT97902830T patent/PT873411E/pt unknown

Also Published As

Publication number	Publication date
WO1997025432A3 (en)	1997-11-06
PT873411E (pt)	2004-08-31
DE69728377D1 (de)	2004-05-06
WO1997025432A2 (en)	1997-07-17
EP0873411B1 (de)	2004-03-31
DE69728377T2 (de)	2005-01-20
DK0873411T3 (da)	2004-07-19
AU719516B2 (en)	2000-05-11
NZ331000A (en)	1999-11-29
CA2242965A1 (en)	1997-07-17
JP3880624B2 (ja)	2007-02-14
US5795761A (en)	1998-08-18
EP0873411A1 (de)	1998-10-28
JPH11514530A (ja)	1999-12-14
AU1691197A (en)	1997-08-01
ES2218659T3 (es)	2004-11-16

Legal Events

Date

Code

Title

Description

2004-12-15

UEP

Publication of translation of european patent specification

Ref document number: 0873411

Country of ref document: EP

2006-10-15

REN

Ceased due to non-payment of the annual fee

Publication	Publication Date	Title
DE69728377D1 (de)	2004-05-06	Verbesserte mutanten von (2,5 dkg) reduktase a
DE69333546D1 (de)	2004-07-15	Verbesserte enzyme zur herstellung von 2-keto-l-gulonsäure
AU9737398A (en)	1999-05-24	Alpha-amylase mutants
EP0165726A3 (de)	1988-01-13	Darmmicroflora-Verbesserungsmittel
FI853519A7 (fi)	1986-03-22	Menetelmä 4,5-dihydro-3(2H) -pyridatsinonin valmistamiseksi ja sen käyttö.
HN1998000143A (es)	1999-04-09	Tetrahidronaftalenos substituidos y compuestos analogos.
ES536909A0 (es)	1985-05-01	Procedimiento para la preparacion de compuestos complejos de piroxicam con ciclodestrinas
DE3789153D1 (de)	1994-04-07	1,2,6-Triphenyl-4(1H)-pyridinon und Pyridinthionderivate, ihre Herstellung und Verwendungen.
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DE3586224D1 (de)	1992-07-23	3-carbonyl-1-aminoalkyl-1h-indole, verwendbar als analgetika und ihre herstellung.
TR199900430T2 (xx)	1999-06-21	�kameli 1,2,3,4-tetrahidro-2-dibenzofuranaminler ve 2-aminosiklohepta$b] benzofuranlar.
DK162839C (da)	1992-05-04	N,3-diacyl-2-oxindol-1-carboxamid-forbindelser og 3-acyl-2-oxindol-forbindelser til anvendelse som mellemprodukter ved deres fremstilling
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