ATE264499T1 - Fluoreszenzintensitätsanalyse unter verwendung einer vielzahl von verteilungen: konkurrierende bestimmung von diffusionszeiten und molekularer helligkeit - Google Patents

Fluoreszenzintensitätsanalyse unter verwendung einer vielzahl von verteilungen: konkurrierende bestimmung von diffusionszeiten und molekularer helligkeit

Info

Publication number
ATE264499T1
ATE264499T1 AT01903729T AT01903729T ATE264499T1 AT E264499 T1 ATE264499 T1 AT E264499T1 AT 01903729 T AT01903729 T AT 01903729T AT 01903729 T AT01903729 T AT 01903729T AT E264499 T1 ATE264499 T1 AT E264499T1
Authority
AT
Austria
Prior art keywords
particles
distributions
variety
fluorescence intensity
intensity analysis
Prior art date
Application number
AT01903729T
Other languages
English (en)
Inventor
Peet Kask
Original Assignee
Evotec Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evotec Ag filed Critical Evotec Ag
Application granted granted Critical
Publication of ATE264499T1 publication Critical patent/ATE264499T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/44—Raman spectrometry; Scattering spectrometry ; Fluorescence spectrometry
    • G01J3/4406—Fluorescence spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J3/457—Correlation spectrometry, e.g. of the intensity
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
    • G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
    • G01J3/28—Investigating the spectrum
    • G01J2003/2866—Markers; Calibrating of scan
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6421—Measuring at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N2021/6463—Optics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence

Landscapes

  • Physics & Mathematics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • General Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
AT01903729T 2000-02-10 2001-02-09 Fluoreszenzintensitätsanalyse unter verwendung einer vielzahl von verteilungen: konkurrierende bestimmung von diffusionszeiten und molekularer helligkeit ATE264499T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US18156400P 2000-02-10 2000-02-10
EP00126774 2000-12-06
PCT/EP2001/001431 WO2001059416A1 (en) 2000-02-10 2001-02-09 Fluorescence intensity multiple distributions analysis: concurrent determination of diffusion times and molecular brightness

Publications (1)

Publication Number Publication Date
ATE264499T1 true ATE264499T1 (de) 2004-04-15

Family

ID=26071648

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01903729T ATE264499T1 (de) 2000-02-10 2001-02-09 Fluoreszenzintensitätsanalyse unter verwendung einer vielzahl von verteilungen: konkurrierende bestimmung von diffusionszeiten und molekularer helligkeit

Country Status (5)

Country Link
EP (1) EP1254353B1 (de)
AT (1) ATE264499T1 (de)
AU (1) AU3172301A (de)
DE (1) DE60102797T2 (de)
WO (1) WO2001059416A1 (de)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007508810A (ja) 2003-09-30 2007-04-12 エヴォテック ニューロサイエンシス ゲゼルシャフト ミット ベシュレンクテル ハフツング スルホトランスフェラーゼの神経変性疾患に関する診断的および治療的使用
EP1528379A1 (de) * 2003-10-27 2005-05-04 Evotec Technologies GmbH Verfahren zum Auflösen von Spezies von fluoreszierenden Molekülen in einer Probe
JP2008508893A (ja) 2004-08-11 2008-03-27 エボテツク・ニユーロサイエンシーズ・ゲー・エム・ベー・ハー 形質膜ａｔｐアーゼの診断及び治療への使用
WO2006125830A2 (en) 2005-05-27 2006-11-30 Evotec Neurosciences Gmbh Kcnn3 as diagnostic and therapeutic target for neurodegenerative diseases
ATE534751T1 (de) 2005-05-30 2011-12-15 Takeda Pharmaceutical Verwendung von prkx zur diagnose und therapie von alzheimer krankheit
US20090133135A1 (en) 2005-06-01 2009-05-21 Evotec Neurosciences Gmbh Diagnostic and Therapeutic Target SLC39A11 Proteins for Neurodegenerative Diseases
WO2006134128A2 (en) 2005-06-16 2006-12-21 Evotec Neurosciences Gmbh Diagnostic and therapeutic target adarb2 proteins for neurodegenerative diseases
EP2053397A1 (de) 2007-10-25 2009-04-29 EVOTEC Neurosciences GmbH PPM1E-Proteine und Nukleinsäuren als Targets für neurodegenerative Erkrankungen
DE102013213362A1 (de) 2013-07-08 2015-01-08 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Verfahren zur Identifizierung und Quantifizierung von emittierenden Teilchen in Systemen

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1595422A (en) * 1977-04-28 1981-08-12 Nat Res Dev Scaning microscopes
EP0884583A1 (de) * 1997-06-10 1998-12-16 Evotec BioSystems GmbH Verfahren zur Charakterisierung von Proben in einem mindestens zweidimensionalen Raum spezifischer physikalischer Eigenschaften
EP1063502A1 (de) * 1999-06-23 2000-12-27 Evotec BioSystems AG Verfahren zur Charakterisierung fluoreszierender Moleküle oder anderer Teilchen unter Verwendung von erzeugenden Funktionen

Also Published As

Publication number Publication date
WO2001059416A1 (en) 2001-08-16
DE60102797T2 (de) 2005-09-08
AU3172301A (en) 2001-08-20
EP1254353A1 (de) 2002-11-06
EP1254353B1 (de) 2004-04-14
DE60102797D1 (de) 2004-05-19

Similar Documents

Publication Publication Date Title
DK1254362T3 (da) Analyse af fluorescensintensitet og levetidsfordeling
ATE235045T1 (de) Verfahren zur erfassung von fluoreszierenden molekülen oder anderen teilchen mittels erzeugenden funktionen
CA1043123A (en) Use of modulated stimulus to improve detection sensitivity for signals from particles in a flow chamber
JP3830167B2 (ja) 試料媒体にある標的粒子の所定の特性を決定するための方法および装置
CA2100890C (en) Particle measurement apparatus
WO2003050518A3 (en) System and method for time correlated multi-photon counting measurements
DK0931254T3 (da) Fremgangsmåde til analyse af prøver ved bestemmelse af en funktion af specifik lysstyrke
DK1042664T3 (da) Fremgangsmåde til påvisning af reaktioner ved hjælp af koincidensanalyse
ATE530891T1 (de) Miniaturisierter zellsortierer
US20240151631A1 (en) Methods for self-triggered flow-cytometers
KR20100087104A (ko) 유동 입자를 식별하는 시스템과 저장매체 및 방법
RU2009145112A (ru) Способ и устройство для измерения ph слабощелочных растворов
JP2012505406A5 (de)
WO2022100236A1 (zh) 一种用于荧光强度峰检测的方法
ATE215694T1 (de) Verfahren zur charakterisierung von proben unter verwendung statistischer zwischendaten
DE60102797D1 (de) Fluoreszenzintensitätsanalyse unter verwendung einer vielzahl von verteilungen: konkurrierende bestimmung von diffusionszeiten und molekularer helligkeit
WO2010101623A1 (en) Flow cytometry system and method for applying gain to flow cytometry data
US6759247B2 (en) Method and apparatus for detecting radiation
US9671401B2 (en) Calibration system for use with lateral flow assay test strips
DE69911433D1 (de) Probenanalyse
JP2006528350A (ja) オーバーラップするスペクトルを有する材料を弁別する方法およびシステム
DE102006026748A1 (de) Analyterfassung unter Verwendung einer Lumineszenzkonzentration
US20040120455A1 (en) Method and apparatus for detecting radiation
MXPA04004436A (es) Metodo de tiempo real para la deteccion y caracterizacion de escama.
JPH11295222A (ja) グロー放電発光分析による微粒子測定方法

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties