ATE266729T1 - Rna polymerase mutanten mit erhöhter stabilität - Google Patents

Rna polymerase mutanten mit erhöhter stabilität

Info

Publication number
ATE266729T1
ATE266729T1 AT99963464T AT99963464T ATE266729T1 AT E266729 T1 ATE266729 T1 AT E266729T1 AT 99963464 T AT99963464 T AT 99963464T AT 99963464 T AT99963464 T AT 99963464T AT E266729 T1 ATE266729 T1 AT E266729T1
Authority
AT
Austria
Prior art keywords
rna polymerase
bacteriophages
bacteriophage
rna
increased stability
Prior art date
Application number
AT99963464T
Other languages
English (en)
Inventor
Hans Liao
Van Bob Gemen
Akio Sugiyama
Original Assignee
Biomerieux Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biomerieux Bv filed Critical Biomerieux Bv
Application granted granted Critical
Publication of ATE266729T1 publication Critical patent/ATE266729T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1247—DNA-directed RNA polymerase (2.7.7.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AT99963464T 1998-12-11 1999-12-07 Rna polymerase mutanten mit erhöhter stabilität ATE266729T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP98204185 1998-12-11
PCT/EP1999/009716 WO2000036112A1 (en) 1998-12-11 1999-12-07 Mutants of rna polymerases with increased stability

Publications (1)

Publication Number Publication Date
ATE266729T1 true ATE266729T1 (de) 2004-05-15

Family

ID=8234452

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99963464T ATE266729T1 (de) 1998-12-11 1999-12-07 Rna polymerase mutanten mit erhöhter stabilität

Country Status (9)

Country Link
US (1) US6524828B1 (de)
EP (1) EP1137781B1 (de)
JP (1) JP4485066B2 (de)
AT (1) ATE266729T1 (de)
AU (1) AU1975200A (de)
CA (1) CA2353792C (de)
DE (1) DE69917322T2 (de)
ES (1) ES2221757T3 (de)
WO (1) WO2000036112A1 (de)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2352173T3 (es) * 1999-12-21 2011-02-16 Rna-Line Oy Arn polimerasas del bacteriófago phi 6-phi 14 y utilización de las mismas.
CA2400049C (en) * 2000-03-07 2010-10-05 Akzo Nobel N.V. Rna polymerase mutants with increased thermostability
WO2004053089A2 (en) * 2002-12-11 2004-06-24 The Research Foundation Of State University Of New York Improved methods and materials for reducing production of aberrant products during rna synthesis
WO2005098038A2 (en) * 2004-03-05 2005-10-20 Research Foundation Of State University Of New York Use of rna polymerase as an information-dependent molecular motor
US7617870B1 (en) 2008-05-14 2009-11-17 Halliburton Energy Services, Inc. Extended cement compositions comprising oil-swellable particles and associated methods
JP2009136153A (ja) * 2007-12-03 2009-06-25 Tosoh Corp 変異型ｒｎａポリメラーゼ
WO2009113718A1 (ja) * 2008-03-11 2009-09-17 東ソー株式会社 機能改善したｒｎａポリメラーゼ変異体
JP5625908B2 (ja) * 2008-08-08 2014-11-19 東ソー株式会社 機能改善したｒｎａポリメラーゼ変異体
US9193959B2 (en) 2010-04-16 2015-11-24 Roche Diagnostics Operations, Inc. T7 RNA polymerase variants with enhanced thermostability
FR2981088B1 (fr) 2011-10-06 2013-11-29 Biomerieux Sa Arn polymerases mutees
FR2984357B1 (fr) 2011-12-16 2016-11-18 Biomerieux Sa Procede d'amplification transcriptionnelle d'acides nucleiques reunissant des etapes de temperatures differentes
US9045740B2 (en) * 2012-02-24 2015-06-02 University Of Massachusetts Modified T7-related RNA polymerases and methods of use thereof
US10034951B1 (en) * 2017-06-21 2018-07-31 New England Biolabs, Inc. Use of thermostable RNA polymerases to produce RNAs having reduced immunogenicity
JP2021040485A (ja) * 2017-11-22 2021-03-18 Ｓｐｉｂｅｒ株式会社 組換えタンパク質の発現システム、発現ベクター、組換え細胞、及び組換えタンパク質の製造方法
CN112831484B (zh) * 2021-01-13 2022-09-20 华中科技大学 T7-rna聚合酶突变体及其应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4952496A (en) 1984-03-30 1990-08-28 Associated Universities, Inc. Cloning and expression of the gene for bacteriophage T7 RNA polymerase
DE69431240T2 (de) 1993-12-01 2003-04-17 Toyo Boseki K.K., Osaka Verfahren zur ampflifizierung und zum nachweis bestimmter nukleinsäuresequenzen mittels thermostabiler enzyme

Also Published As

Publication number Publication date
EP1137781B1 (de) 2004-05-12
ES2221757T3 (es) 2005-01-01
EP1137781A1 (de) 2001-10-04
WO2000036112A1 (en) 2000-06-22
US6524828B1 (en) 2003-02-25
JP2002532095A (ja) 2002-10-02
JP4485066B2 (ja) 2010-06-16
DE69917322D1 (de) 2004-06-17
AU1975200A (en) 2000-07-03
CA2353792A1 (en) 2000-06-22
CA2353792C (en) 2007-11-27
DE69917322T2 (de) 2005-05-04

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