ATE294855T1 - Rna polymerase mutanten mit erhöhter thermostabilität - Google Patents

Rna polymerase mutanten mit erhöhter thermostabilität

Info

Publication number: ATE294855T1
Authority: AT; Austria
Prior art keywords: rna polymerase; increased thermostability; polymerase mutants; rna polymerases; bacteriophages
Prior art date: 2000-03-07

Application number

AT01909810T

Other languages

English (en)

Inventor

Akio Sugiyama

Yoshiaki Nishiya

Bunsei Kawakami

Original Assignee

Biomerieux Bv

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2000-03-07

Filing date

2001-03-01

Publication date

2005-05-15

2001-03-01 Application filed by Biomerieux Bv filed Critical Biomerieux Bv

2005-05-15 Application granted granted Critical

2005-05-15 Publication of ATE294855T1 publication Critical patent/ATE294855T1/de

Links

102000004163 DNA-directed RNA polymerases Human genes 0.000 title abstract 4
108090000626 DNA-directed RNA polymerases Proteins 0.000 title abstract 4
241001515965 unidentified phage Species 0.000 abstract 2
ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 abstract 1
MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 abstract 1
101710137500 T7 RNA polymerase Proteins 0.000 abstract 1
150000001413 amino acids Chemical group 0.000 abstract 1
-1 proline amino acid Chemical class 0.000 abstract 1
108090000623 proteins and genes Proteins 0.000 abstract 1
102000004169 proteins and genes Human genes 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1247—DNA-directed RNA polymerase (2.7.7.6)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
- C12Y207/07006—DNA-directed RNA polymerase (2.7.7.6)

Landscapes

Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Molecular Biology (AREA)
Engineering & Computer Science (AREA)
Bioinformatics & Cheminformatics (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Genetics & Genomics (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
General Engineering & Computer Science (AREA)
Medicinal Chemistry (AREA)
Biomedical Technology (AREA)
Biotechnology (AREA)
Microbiology (AREA)
Enzymes And Modification Thereof (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Heat Sensitive Colour Forming Recording (AREA)

AT01909810T 2000-03-07 2001-03-01 Rna polymerase mutanten mit erhöhter thermostabilität ATE294855T1 (de)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
EP00200787		2000-03-07
PCT/EP2001/002327 WO2001066705A1 (en)	2000-03-07	2001-03-01	Rna polymerase mutants with increased thermostability

Publications (1)

Publication Number	Publication Date
ATE294855T1 true ATE294855T1 (de)	2005-05-15

Family

ID=8171152

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
AT01909810T ATE294855T1 (de)	2000-03-07	2001-03-01	Rna polymerase mutanten mit erhöhter thermostabilität

Country Status (9)

Country	Link
US (1)	US7507567B2 (de)
EP (1)	EP1261696B1 (de)
JP (1)	JP4832694B2 (de)
AT (1)	ATE294855T1 (de)
AU (1)	AU2001237425A1 (de)
CA (1)	CA2400049C (de)
DE (1)	DE60110564T2 (de)
ES (1)	ES2242733T3 (de)
WO (1)	WO2001066705A1 (de)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JP2009136153A (ja) *	2007-12-03	2009-06-25	Tosoh Corp	変異型ｒｎａポリメラーゼ
WO2009113718A1 (ja) *	2008-03-11	2009-09-17	東ソー株式会社	機能改善したｒｎａポリメラーゼ変異体
JP5625908B2 (ja) *	2008-08-08	2014-11-19	東ソー株式会社	機能改善したｒｎａポリメラーゼ変異体
US9193959B2 (en)	2010-04-16	2015-11-24	Roche Diagnostics Operations, Inc.	T7 RNA polymerase variants with enhanced thermostability
EP2611922A1 (de)	2010-08-31	2013-07-10	Greenlight Biosciences, Inc.	Verfahren zur flusssteuerung bei stoffwechselwegen durch proteasemanipulation
US9062292B2 (en)	2010-09-13	2015-06-23	Enzo Life Sciences Inc.	Mutant T7 polymerases
FR2981088B1 (fr)	2011-10-06	2013-11-29	Biomerieux Sa	Arn polymerases mutees
US9045740B2 (en) *	2012-02-24	2015-06-02	University Of Massachusetts	Modified T7-related RNA polymerases and methods of use thereof
AU2014306074B2 (en)	2013-08-05	2018-08-30	Greenlight Biosciences, Inc.	Engineered proteins with a protease cleavage site
WO2015024017A2 (en) *	2013-08-16	2015-02-19	President And Fellows Of Harvard College	Rna polymerase, methods of purification and methods of use
KR20160132113A (ko)	2014-03-20	2016-11-16	보드 오브 리전츠, 더 유니버시티 오브 텍사스 시스템	확장된 기질 범위 및 증진된 전사 수율을 갖는 t7 rna 폴리머라제 변이체
BR112017020690A2 (pt)	2015-03-30	2018-06-26	Greenlight Biosciences Inc	produção livre de células de ácido ribonucleico
EP3317424B1 (de) *	2015-07-01	2023-09-06	CureVac Manufacturing GmbH	Verfahren zur analyse eines rna-moleküls
CN116144626A (zh) *	2016-01-13	2023-05-23	新英格兰生物实验室公司	T7 rna聚合酶的热稳定变体
CA3020312A1 (en)	2016-04-06	2017-10-12	Greenlight Biosciences, Inc.	Cell-free production of ribonucleic acid
US10034951B1 (en)	2017-06-21	2018-07-31	New England Biolabs, Inc.	Use of thermostable RNA polymerases to produce RNAs having reduced immunogenicity
KR20250028535A (ko)	2017-06-30	2025-02-28	코덱시스, 인코포레이티드	T7 rna 폴리머라제 변이체
EP3645712A4 (de)	2017-06-30	2021-07-07	Codexis, Inc.	T7-rna-polymerasevarianten
KR102894284B1 (ko)	2017-10-11	2025-12-03	그린라이트 바이오사이언시스, 아이엔씨.	뉴클레오시드 트리포스페이트 및 리보핵산 생산을 위한 방법 및 조성물
CN112218947A (zh) *	2018-04-10	2021-01-12	绿光生物科技股份有限公司	T7 rna聚合酶变体
WO2020243026A1 (en) *	2019-05-24	2020-12-03	Primordial Genetics Inc.	Methods and compositions for manufacturing polynucleotides
CN112980811B (zh) *	2021-03-06	2022-02-25	苏州瀚源新酶生物科技有限公司	Rna聚合酶突变体及其应用、重组载体及其制备方法和应用、重组工程菌及其应用
EP4108770A1 (de)	2021-06-24	2022-12-28	Aptamimetics GmbH	Age-selex-verfahren zur identifizierung und verwendung von aptameren
WO2025127053A1 (ja) *	2023-12-12	2025-06-19	タカラバイオ株式会社	Ｒｎａポリメラーゼ変異体とｒｎａ合成

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4952496A (en) *	1984-03-30	1990-08-28	Associated Universities, Inc.	Cloning and expression of the gene for bacteriophage T7 RNA polymerase
CA1340807C (en)	1988-02-24	1999-11-02	Lawrence T. Malek	Nucleic acid amplification process
US5385834A (en) *	1993-08-13	1995-01-31	Georgia Tech Research Corporation	Mutant T7 RNA polymerase GP1(lys222) exhibiting altered promoter recognition
FR2761695B1 (fr) *	1997-04-04	1999-09-24	Bio Merieux	Arn polymerase fonctionnant preferentiellement sur matrice d'arn, sa preparation et son utilisation
JP3172710B2 (ja) *	1997-07-07	2001-06-04	理化学研究所	Ｒｎａポリメラーゼ
ATE266729T1 (de) *	1998-12-11	2004-05-15	Biomerieux Bv	Rna polymerase mutanten mit erhöhter stabilität
JP4399684B2 (ja) *	1999-08-17	2010-01-20	東洋紡績株式会社	改良されたｒｎａポリメラーゼ

2001
- 2001-03-01 CA CA2400049A patent/CA2400049C/en not_active Expired - Lifetime
- 2001-03-01 DE DE60110564T patent/DE60110564T2/de not_active Expired - Lifetime
- 2001-03-01 ES ES01909810T patent/ES2242733T3/es not_active Expired - Lifetime
- 2001-03-01 AU AU2001237425A patent/AU2001237425A1/en not_active Abandoned
- 2001-03-01 EP EP01909810A patent/EP1261696B1/de not_active Expired - Lifetime
- 2001-03-01 WO PCT/EP2001/002327 patent/WO2001066705A1/en not_active Ceased
- 2001-03-01 AT AT01909810T patent/ATE294855T1/de active
- 2001-03-01 JP JP2001565862A patent/JP4832694B2/ja not_active Expired - Lifetime
- 2001-03-01 US US10/220,908 patent/US7507567B2/en not_active Expired - Lifetime

Also Published As

Publication number	Publication date
DE60110564T2 (de)	2006-01-19
EP1261696B1 (de)	2005-05-04
EP1261696A1 (de)	2002-12-04
JP2003525627A (ja)	2003-09-02
JP4832694B2 (ja)	2011-12-07
US7507567B2 (en)	2009-03-24
CA2400049A1 (en)	2001-09-13
ES2242733T3 (es)	2005-11-16
WO2001066705A1 (en)	2001-09-13
US20030175738A1 (en)	2003-09-18
CA2400049C (en)	2010-10-05
DE60110564D1 (en)	2005-06-09
AU2001237425A1 (en)	2001-09-17

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2006-01-15

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