ATE267037T1 - Prozess zur chromatographischen trennung von peptiden und nucleinsaure und neue ionentauschermatrix mit hoher affinitat - Google Patents

Prozess zur chromatographischen trennung von peptiden und nucleinsaure und neue ionentauschermatrix mit hoher affinitat

Info

Publication number
ATE267037T1
ATE267037T1 AT97903706T AT97903706T ATE267037T1 AT E267037 T1 ATE267037 T1 AT E267037T1 AT 97903706 T AT97903706 T AT 97903706T AT 97903706 T AT97903706 T AT 97903706T AT E267037 T1 ATE267037 T1 AT E267037T1
Authority
AT
Austria
Prior art keywords
pct
peptides
ion exchange
anion exchanger
high affinity
Prior art date
Application number
AT97903706T
Other languages
German (de)
English (en)
Inventor
Rolf Berglund
Jan Bergstroem
Makonnen Belew
Lennart Soederberg
Original Assignee
Amersham Biosciences Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amersham Biosciences Ab filed Critical Amersham Biosciences Ab
Application granted granted Critical
Publication of ATE267037T1 publication Critical patent/ATE267037T1/de

Links

Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D15/00Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08Selective adsorption, e.g. chromatography
    • B01D15/26Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361Ion-exchange
    • B01D15/363Anion-exchange
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J41/00Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
    • B01J41/04Processes using organic exchangers
    • B01J41/07Processes using organic exchangers in the weakly basic form
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J41/00Anion exchange; Use of material as anion exchangers; Treatment of material for improving the anion exchange properties
    • B01J41/20Anion exchangers for chromatographic processes
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/16Extraction; Separation; Purification by chromatography
    • C07K1/18Ion-exchange chromatography
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2220/00Aspects relating to sorbent materials
    • B01J2220/50Aspects relating to the use of sorbent or filter aid materials
    • B01J2220/54Sorbents specially adapted for analytical or investigative chromatography

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
AT97903706T 1996-02-19 1997-02-14 Prozess zur chromatographischen trennung von peptiden und nucleinsaure und neue ionentauschermatrix mit hoher affinitat ATE267037T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
SE9600590A SE9600590D0 (sv) 1996-02-19 1996-02-19 Sätt för kromatografisk separation av peptider och nukleinsyra samt ny högaffin jonbytesmatris
PCT/SE1997/000237 WO1997029825A1 (en) 1996-02-19 1997-02-14 Process for chromatographic separation of peptides and nucleic acid, and new high affinity ion exchange matrix

Publications (1)

Publication Number Publication Date
ATE267037T1 true ATE267037T1 (de) 2004-06-15

Family

ID=20401430

Family Applications (1)

Application Number Title Priority Date Filing Date
AT97903706T ATE267037T1 (de) 1996-02-19 1997-02-14 Prozess zur chromatographischen trennung von peptiden und nucleinsaure und neue ionentauschermatrix mit hoher affinitat

Country Status (8)

Country Link
US (1) US6090288A (2)
EP (1) EP0888157B1 (2)
JP (1) JP4422797B2 (2)
AT (1) ATE267037T1 (2)
AU (1) AU713635B2 (2)
DE (1) DE69729182T2 (2)
SE (1) SE9600590D0 (2)
WO (1) WO1997029825A1 (2)

Families Citing this family (70)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9425138D0 (en) * 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
DE19520398B4 (de) 1995-06-08 2009-04-16 Roche Diagnostics Gmbh Magnetisches Pigment
KR100463475B1 (ko) 1995-06-08 2005-06-22 로셰 디아그노스틱스 게엠베하 자기성피그먼트
US6027945A (en) 1997-01-21 2000-02-22 Promega Corporation Methods of isolating biological target materials using silica magnetic particles
SE9700769D0 (sv) 1997-03-04 1997-03-04 Pharmacia Biotech Ab Matriser för separation och separation som utnyttjar matriserna
DE19736366A1 (de) * 1997-08-21 1999-02-25 Ernst Prof Dr Bayer Verfahren zur Isolierung von anionischen organischen Verbindungen aus wäßrigen Systemen mit kationischen Polymernanopartikeln
WO1999029703A2 (en) * 1997-12-06 1999-06-17 Dna Research Instruments Limited Isolation of nucleic acids
US6914137B2 (en) * 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
US7078224B1 (en) 1999-05-14 2006-07-18 Promega Corporation Cell concentration and lysate clearance using paramagnetic particles
SE9802214D0 (sv) * 1998-06-18 1998-06-18 Amersham Pharm Biotech Ab Jonbytare och användning därav
WO2000034521A1 (en) * 1998-12-08 2000-06-15 Boston Probes, Inc. Methods, kits and compositions for the identification of nucleic acids electrostatically bound to matrices
EP1147226B1 (en) * 1999-01-27 2013-01-23 Folim G. Halaka Materials and methods for the purification of polyelectrolytes
US6310199B1 (en) 1999-05-14 2001-10-30 Promega Corporation pH dependent ion exchange matrix and method of use in the isolation of nucleic acids
US6270970B1 (en) 1999-05-14 2001-08-07 Promega Corporation Mixed-bed solid phase and its use in the isolation of nucleic acids
SE9904197D0 (sv) 1999-11-22 1999-11-22 Amersham Pharm Biotech Ab A method for anion exchange adsorption on matrices carrying mixed mode ligands
SE9904272D0 (sv) * 1999-11-25 1999-11-25 Amersham Pharm Biotech Ab A method for selective removal of a substance from samples containing compounds having nucleic acid structure
GB9927904D0 (en) * 1999-11-25 2000-01-26 Amersham Pharm Biotech Ab A method fro obtaining a nucleic acid variant
ES2277910T3 (es) * 2000-02-08 2007-08-01 Upfront Chromatography A/S Nuevos materiales de resina y su uso para recuperar proteinas o peptidos.
US20020194901A1 (en) * 2000-12-19 2002-12-26 Showa Denko K.K. Porous polymer particle, alkali-resistant anion exchanger, producing method thereof, column for ion chromatography, and method for measuring anions
US20030066336A1 (en) * 2000-12-19 2003-04-10 Showa Denko K.K. Method for measuring anions
SE0004932D0 (sv) * 2000-12-31 2000-12-31 Apbiotech Ab A method for mixed mode adsorption and mixed mode adsorbents
DE60236951D1 (de) * 2001-05-21 2010-08-19 Fujifilm Corp Vorrichtung zur Durchführung biochemischer Analysen und Verfahren zu ihrer Herstellung
SE0103084D0 (sv) * 2001-09-14 2001-09-14 Amersham Pharm Biotech Ab Generation of ion exchange media
GB0127809D0 (en) * 2001-11-20 2002-01-09 Dna Res Innovations Ltd Purification of nucleic acid
JP2005538929A (ja) 2002-01-16 2005-12-22 ダイナル バイオテック エイエスエイ 単一サンプルからの核酸及びタンパク質の単離方法
US20040029143A1 (en) * 2002-02-28 2004-02-12 Jeffrey Van Ness Cationic polyelectrolytes in biomolecule purification and analysis
US20060035224A1 (en) * 2002-03-21 2006-02-16 Johansen Jack T Purification methods for oligonucleotides and their analogs
GB0207975D0 (en) * 2002-04-05 2002-05-15 Genovision As Isolating nucleic acid
GB0212826D0 (en) 2002-05-31 2002-07-10 Dna Res Innovations Ltd Materials and methods relating to polyions and substance delivery
US6989442B2 (en) * 2002-07-12 2006-01-24 Sirna Therapeutics, Inc. Deprotection and purification of oligonucleotides and their derivatives
US7320754B2 (en) 2003-03-05 2008-01-22 Ge Healthcare Bio-Sciences Ab Method of preparing multi-modal anion-exchange ligands
SE0302911D0 (sv) * 2003-10-31 2003-10-31 Amersham Biosciences Ab Novel separation matrix
US20050106589A1 (en) * 2003-11-17 2005-05-19 Hashem Akhavan-Tafti Compositions and methods for releasing nucleic acids from solid phase binding materials
SE0303532D0 (sv) * 2003-12-23 2003-12-23 Amersham Biosciences Ab Purification of immunoglobulins
EP2821135A1 (en) * 2004-02-05 2015-01-07 EMD Millipore Corporation Porous adsorptive or chromatographic media
EP1693108A1 (en) 2004-12-04 2006-08-23 MERCK PATENT GmbH Mixed-modal anion-exchange type separation material
KR100668337B1 (ko) 2005-05-21 2007-01-12 삼성전자주식회사 단백질에 비하여 핵산에 대한 결합력이 선택적으로 높은pH 의존성 이온 교환물질, 그가 고정화되어 있는 고체기판, 및 상기 물질 및 고체 기판을 이용하여 핵산을분리하는 방법
CA2617645C (en) 2005-08-03 2014-09-09 Merck Patent Gesellschaft Mit Beschraenkter Haftung Hydrophilic crosslinked polymer
EP1754534A1 (de) 2005-08-03 2007-02-21 MERCK PATENT GmbH Hydrophiles vernetztes Polymer
GB2445441B (en) * 2006-09-26 2010-06-30 Ge Healthcare Bio Sciences Nucleic acid purification method
GB2445442A (en) 2006-09-26 2008-07-09 Ge Healthcare Bio Sciences Nucleic acid purification using anion exchange
GB2443505B (en) * 2006-09-26 2008-12-31 Ge Healthcare Bio Sciences Nucleic acid purification method
DE202008017854U1 (de) 2007-08-14 2010-08-19 Millipore Corp., Billerica Mittel zur Ionenaustausch-Membranchromatographie auf der Basis von primären polymerischen Aminen, Sorptionsvorrichtung mit diesem Mittel, Chromatographieverfahren und Reinigungsverfahren unter dessen Verwendung
US9433922B2 (en) 2007-08-14 2016-09-06 Emd Millipore Corporation Media for membrane ion exchange chromatography based on polymeric primary amines, sorption device containing that media, and chromatography scheme and purification method using the same
US20090130738A1 (en) * 2007-11-19 2009-05-21 Mikhail Kozlov Media for membrane ion exchange chromatography
US8067580B2 (en) * 2008-02-07 2011-11-29 Ge Healthcare Bio-Sciences Corp. Isolation of DNA, RNA and protein from a single sample
US8188242B2 (en) 2008-04-08 2012-05-29 Bio-Rad Laboratories, Inc. Chromatography purification of antibodies
WO2009145722A1 (en) * 2008-05-30 2009-12-03 Ge Healthcare Bio-Sciences Ab Separation method utilizing polyallylamine ligands
CN103314290B (zh) * 2011-01-12 2015-07-01 积水医疗株式会社 核酸链的分离方法
WO2012096329A1 (ja) 2011-01-12 2012-07-19 積水メディカル株式会社 一塩基多型の検出方法
EP2674753B1 (en) * 2011-02-10 2020-05-13 Sekisui Medical Co., Ltd. Method for separating and detecting a nucleic acid strand
CN104812491B (zh) * 2012-03-08 2018-06-19 生物辐射实验室股份有限公司 阴离子交换-疏水性混合模式
US9169304B2 (en) 2012-05-01 2015-10-27 Pfenex Inc. Process for purifying recombinant Plasmodium falciparum circumsporozoite protein
WO2014043644A1 (en) * 2012-09-17 2014-03-20 Alltech Associates, Inc. Functionalized particulate support material and methods of making and using the same
RU2015114330A (ru) 2012-09-17 2016-11-10 У.Р. Грейс Энд Ко.-Конн. Хроматографические среды и устройства
EP2925432A4 (en) * 2012-11-29 2016-08-31 Affigel Ab SUPER-HYDROXYLATED ADSORBENTS AND USES THEREOF
US11229896B2 (en) 2014-01-16 2022-01-25 W.R. Grace & Co.—Conn. Affinity chromatography media and chromatography devices
WO2015137860A1 (en) * 2014-03-14 2015-09-17 Ge Healthcare Bio-Sciences Ab Separation matrices for purification of biological particles
JP6914189B2 (ja) 2014-05-02 2021-08-04 ダブリュー・アール・グレース・アンド・カンパニー−コーンW R Grace & Co−Conn 官能化担体材料並びに官能化担体材料を作製及び使用する方法
SG11201609908VA (en) 2014-05-28 2016-12-29 Agency Science Tech & Res Virus reduction method
WO2016172496A1 (en) 2015-04-23 2016-10-27 Constellation Pharmaceuticals, Inc. Lsd1 inhibitors and uses thereof
CN107921407B (zh) 2015-06-05 2022-07-05 格雷斯公司 生物处理吸附澄清剂及其制备和使用方法
CN109715648A (zh) * 2016-09-15 2019-05-03 克拉维格两合股份有限公司 聚合网状物用于大分子纯化的用途
DK3532459T3 (da) 2016-10-26 2023-10-30 Constellation Pharmaceuticals Inc Lsd1-hæmmere og medicinske anvendelser deraf
CN110709696B (zh) * 2017-06-22 2022-01-04 昭和电工株式会社 寡核苷酸的混合物的分离分析方法
EP3749697A4 (en) 2018-02-05 2021-11-03 Bio-Rad Laboratories, Inc. CHROMATOGRAPHY RESIN WITH LIGAND MIXED MODE ANIONIC / HYDROPHOBIC EXCHANGE
US12343702B2 (en) 2018-03-08 2025-07-01 Bio-Rad Laboratories, Inc. Anionic exchange-hydrophobic mixed mode chromatography resin
EP4025336A4 (en) 2019-09-05 2024-01-17 Bio-Rad Laboratories, Inc. ANION EXCHANGE/HYDROPHOBIC RESINS FOR MIXED MODE CHROMATOGRAPHY
EP4070102A1 (en) 2019-12-05 2022-10-12 Waters Technologies Corporation Polyphosphonic acids for improving recovery and minimizing system loss
EP4182460A1 (en) * 2020-07-14 2023-05-24 Waters Technologies Corporation Kits and methods useful for separating oligonucleotides from matrix components

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4767670A (en) * 1987-01-21 1988-08-30 E. I. Du Pont De Nemours And Company Chromatographic supports for separation of oligonucleotides
SE8902315D0 (sv) * 1989-06-27 1989-06-27 Pharmacia Ab Anjonbytare
ATE103507T1 (de) * 1989-08-07 1994-04-15 Baker J T Inc Feste traeger aus quaternisierter pei-kieselsaeure fuer die chromatographie.
US5087359A (en) * 1989-08-07 1992-02-11 J. T. Baker Inc. Quaternized PEI silica solid supports for chromatography
SE9004129D0 (sv) * 1990-12-21 1990-12-21 Pharmacia Lkb Biotech Anion exchanger

Also Published As

Publication number Publication date
AU713635B2 (en) 1999-12-09
JP4422797B2 (ja) 2010-02-24
US6090288A (en) 2000-07-18
DE69729182T2 (de) 2005-01-27
DE69729182D1 (de) 2004-06-24
AU1818097A (en) 1997-09-02
SE9600590D0 (sv) 1996-02-19
JP2000504625A (ja) 2000-04-18
EP0888157B1 (en) 2004-05-19
WO1997029825A1 (en) 1997-08-21
EP0888157A1 (en) 1999-01-07

Similar Documents

Publication Publication Date Title
ATE267037T1 (de) Prozess zur chromatographischen trennung von peptiden und nucleinsaure und neue ionentauschermatrix mit hoher affinitat
DE69611445D1 (de) Verfahren zum herstellen von höchstreinem albumin
CA2099138A1 (en) Chondroitinase, Process for Preparing the Same, and Pharmaceutical Composition Comprising the Same
ATE274522T1 (de) Abtrennung von antikörper-monomeren von deren multimeren mittels ionaustausch-chromatographie
KR900701823A (ko) 혈장단백질의 크로마토그래피 분리법
DE69638291D1 (de) Verfahren zur Reinigung von Faktor IX
DE60132075D1 (de) Wnt-1 verwandte polypeptide und dafür kodierende nukleinsäuren
AU4677193A (en) Plasma fraction purification
RU97104486A (ru) Трофические факторы для регенерации центральной нервной системы
ATE420186T1 (de) Verfahren zur reinigung von an mehreren epitopen fusionierten rekombinanten proteinen
Kanno et al. Relationship of soluble glycoprotein of milk fat globule membrane to component-3,-5 and-8 fractions of proteose-peptone
EP0931834A3 (en) Echinocandin binding domain of 1,3-Beta-glucan synthase
WO1998029540A3 (de) REKOMBINANTE FUSIONSPROTEINE AUF DER BASIS RIBOSOMEN-INAKTIVIERENDER PROTEINE DER MISTEL $i(VISCUM ALBUM)
WO2000006706A3 (en) Map kinase phosphatase mutant
CA2242927A1 (en) Process for chromatographic separation of peptides and nucleic acid, and new high affinity ion exchange matrix
Fullmer et al. The effect of calcium on the tryptic digestion of bovine intestinal calcium-binding protein
Garg et al. Therapeutic Grade Proteins
EP1916256A3 (en) Search for cancer markers by a novel screening method
SE9302856D0 (sv) Method and means for producing a plasmapeotein-binding protein
Farbiszewski et al. Characterization of cytoplasmic arginine-rich basic protein of Guerin epithelioma
Khanaka et al. Comparative study of the DNA-binding HU-type proteins from slow growing and fast growing strains of Rhizobiaceae
Reuben Protein chromatography based upon combined pi-electron and electrostatic interactions
CA2193867A1 (en) Hydrophobic chromatographic resins with ionizable groups
Smith An experimental approach to determine the binding mode of yeast linker histone, Hho1p
Hachtel Biosynthesis and Membrane Insertion of a Chlorophyll a/b-binding Protein by Isolated Vicia faba L., Chloroplasts

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties