ATE420186T1 - Verfahren zur reinigung von an mehreren epitopen fusionierten rekombinanten proteinen - Google Patents

Verfahren zur reinigung von an mehreren epitopen fusionierten rekombinanten proteinen

Info

Publication number
ATE420186T1
ATE420186T1 AT00966755T AT00966755T ATE420186T1 AT E420186 T1 ATE420186 T1 AT E420186T1 AT 00966755 T AT00966755 T AT 00966755T AT 00966755 T AT00966755 T AT 00966755T AT E420186 T1 ATE420186 T1 AT E420186T1
Authority
AT
Austria
Prior art keywords
identification
polypeptides
dna
identification polypeptides
purification
Prior art date
Application number
AT00966755T
Other languages
English (en)
Inventor
Bill Brizzard
Ron Hernan
Original Assignee
Sigma Aldrich Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sigma Aldrich Co filed Critical Sigma Aldrich Co
Application granted granted Critical
Publication of ATE420186T1 publication Critical patent/ATE420186T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/001—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof by chemical synthesis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/08—Linear peptides containing only normal peptide links having 12 to 20 amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C07K2319/43—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a FLAG-tag

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AT00966755T 1999-10-08 2000-09-20 Verfahren zur reinigung von an mehreren epitopen fusionierten rekombinanten proteinen ATE420186T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/415,000 US6379903B1 (en) 1999-10-08 1999-10-08 Purification of recombinant proteins fused to multiple epitopes

Publications (1)

Publication Number Publication Date
ATE420186T1 true ATE420186T1 (de) 2009-01-15

Family

ID=23643935

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00966755T ATE420186T1 (de) 1999-10-08 2000-09-20 Verfahren zur reinigung von an mehreren epitopen fusionierten rekombinanten proteinen

Country Status (9)

Country Link
US (3) US6379903B1 (de)
EP (2) EP2075336B1 (de)
JP (1) JP4405125B2 (de)
AT (1) ATE420186T1 (de)
AU (1) AU774216B2 (de)
CA (1) CA2386471C (de)
DE (1) DE60041345D1 (de)
ES (1) ES2321058T7 (de)
WO (1) WO2001027293A1 (de)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6960457B1 (en) * 1997-09-04 2005-11-01 Stanford University Reversible immobilization of arginine-tagged moieties on a silicate surface
DE10054055A1 (de) * 2000-10-31 2002-05-23 Nmi Univ Tuebingen Verfahren zur Analyse von Proteinen
US7799561B2 (en) * 2002-06-12 2010-09-21 Sigma-Aldrich, Co. Affinity peptides and method for purification of recombinant proteins
GB2395557A (en) * 2002-11-22 2004-05-26 Dynal Biotech Ltd Nucleic acid probes
JP4644663B2 (ja) * 2003-06-03 2011-03-02 セル ジェネシス インコーポレイテッド ペプチド開裂部位を用いた単一ベクターからの組換えポリペプチドの高められた発現のための構成と方法
US7741053B2 (en) * 2005-05-13 2010-06-22 Sigma-Aldrich Co. Processes for purification of recombinant proteins
US20080039616A1 (en) * 2005-09-29 2008-02-14 Sigma-Aldrich Co. Tandem affinity purification systems and methods utilizing such systems
US7855057B2 (en) * 2006-03-23 2010-12-21 Millipore Corporation Protein splice variant/isoform discrimination and quantitative measurements thereof
EP2046818A4 (de) * 2006-07-11 2010-03-03 Leap Biosciences Corp Verfahren für selektive proteinanreicherung und entsprechende anwendungen
US20100062461A1 (en) * 2006-07-11 2010-03-11 Leap Biosciences Corporation Multiplex detection of cell surface receptors or immobilized antigens
WO2016011070A2 (en) * 2014-07-14 2016-01-21 The Regents Of The University Of California A protein tagging system for in vivo single molecule imaging and control of gene transcription
MX2020008272A (es) 2018-02-08 2020-09-21 Sangamo Therapeutics Inc Nucleasas diseñadas específicas de blancos.
EP3826664A4 (de) 2019-02-06 2022-10-26 Sangamo Therapeutics, Inc. Verfahren zur behandlung von mukopolysaccharidose typ i
WO2020205838A1 (en) 2019-04-02 2020-10-08 Sangamo Therapeutics, Inc. Methods for the treatment of beta-thalassemia
US11492380B2 (en) * 2019-07-24 2022-11-08 Lawrence Livermore National Security, Llc PepCon proteomics standards and methods of use
CA3159620A1 (en) 2019-11-01 2021-05-06 Sangamo Therapeutics, Inc. Compositions and methods for genome engineering
EP3988659A1 (de) 2020-10-26 2022-04-27 Wageningen Universiteit Prokaryotische post-stop-codon-sequenzen
EP4083204A1 (de) 2021-04-30 2022-11-02 Wageningen Universiteit Genweite randomisierung

Family Cites Families (44)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2466484A (en) * 1946-11-22 1949-04-05 American Telephone & Telegraph Subscriber ringing circuit
US2632207A (en) * 1949-07-02 1953-03-24 Neumann L Mahla Molding apparatus for rapid cycle molding of thermoplastic material
US4468464A (en) 1974-11-04 1984-08-28 The Board Of Trustees Of The Leland Stanford Junior University Biologically functional molecular chimeras
US4366246A (en) 1977-11-08 1982-12-28 Genentech, Inc. Method for microbial polypeptide expression
US4563424A (en) 1979-11-05 1986-01-07 Genentech, Inc. Method and means for somatostatin protein conjugate expression
US4571421A (en) 1979-11-05 1986-02-18 Genentech, Inc. Mammalian gene for microbial expression
CA1200773A (en) 1980-02-29 1986-02-18 William J. Rutter Expression linkers
US4650756A (en) 1981-08-31 1987-03-17 Sloan Kettering Institute For Cancer Research Monoclonal antibodies to cell surface antigens of human renal cancer
US4532207A (en) 1982-03-19 1985-07-30 G. D. Searle & Co. Process for the preparation of polypeptides utilizing a charged amino acid polymer and exopeptidase
US4468484A (en) * 1982-05-17 1984-08-28 Laclede Professional Products, Inc. Settable alignate compositions containing polyacrylamide
US4503142A (en) 1982-06-25 1985-03-05 Litton Bionetics, Inc. Open reading frame vectors
US4681848A (en) 1982-09-22 1987-07-21 Takeda Chemical Industries, Ltd. Novel peptide and use thereof
US4559302A (en) 1982-11-01 1985-12-17 Eli Lilly And Company DNA for directing transcription and expression of structural genes
US4632207A (en) * 1983-10-05 1986-12-30 Ford Motor Company Mechanism to engage part time drive system in a moving vehicle
US4703004A (en) 1984-01-24 1987-10-27 Immunex Corporation Synthesis of protein with an identification peptide
US4782137A (en) 1984-01-24 1988-11-01 Immunex Corporation Synthesis of protein with an identification peptide, and hybrid polypeptide incorporating same
US4603142A (en) * 1984-06-01 1986-07-29 Wisconsin Alumni Research Foundation Cholesterol lowering method of use
US4650758A (en) * 1984-09-21 1987-03-17 Cetus Corporation Stabilization of enzymes useful in the production of glucosone and other enzymatic processes
US4569794A (en) 1984-12-05 1986-02-11 Eli Lilly And Company Process for purifying proteins and compounds useful in such process
US4978621A (en) 1985-09-03 1990-12-18 Scripps Clinic And Research Foundation Merozoite surface antigens
US4671421A (en) * 1986-03-06 1987-06-09 Owens-Illinois, Inc. Plastic container
US4810632A (en) 1986-12-01 1989-03-07 Scripps Clinic And Research Foundation Cell surface antigen detection method
US5176998A (en) 1986-12-01 1993-01-05 The Scripps Research Institute Cell surface antigen detection method
US4851341A (en) 1986-12-19 1989-07-25 Immunex Corporation Immunoaffinity purification system
US5011912A (en) 1986-12-19 1991-04-30 Immunex Corporation Hybridoma and monoclonal antibody for use in an immunoaffinity purification system
CA1340522C (en) 1987-03-10 1999-05-04 Heinz Dobeli Fusion proteins containing neighbouring histidines for improved purification
US4878621A (en) * 1987-06-16 1989-11-07 Krueger Michael J Projecting gun and nozzle
FR2628750B1 (fr) 1988-03-21 1991-11-08 Pasteur Institut Vecteur modifie par une sequence codant pour une sequence d'acides amines contenue dans une proteine de mammifere ayant une activite biologique de recepteur membranaire
US5298599A (en) * 1988-12-30 1994-03-29 Oklahoma Medical Research Foundation Expression and purification of recombinant soluble tissue factor
DE3906540A1 (de) * 1989-03-02 1990-09-13 Behringwerke Ag Expressionsvektoren zur synthese von proteinen in der spalthefe schizosaccharomyces pombe
US5124251A (en) 1990-01-31 1992-06-23 Becton, Dickinson And Company CD3 ζ co-associated complex on CD16- NK cells
US5594115A (en) 1990-04-09 1997-01-14 Pharmacia & Upjohn Company Process of purifying recombinant proteins and compounds useful in such process
US5571894A (en) * 1991-02-05 1996-11-05 Ciba-Geigy Corporation Recombinant antibodies specific for a growth factor receptor
US5185438A (en) 1991-04-02 1993-02-09 The Trustees Of Princeton University Nucleic acids encoding hencatoporetic stem cell receptor flk-2
US5270458A (en) 1991-04-02 1993-12-14 The Trustees Of Princeton University Nucleic acids encoding fragments of hematopoietic stem cell receptor flk-2
WO1994012206A1 (en) * 1992-12-03 1994-06-09 Magainin Pharmaceuticals, Inc. Treatment of dermatological malignancies with biologically active peptides
US5731425A (en) 1994-10-28 1998-03-24 Eastman Kodak Company Polypeptide surface marker for cells
US5800811A (en) * 1995-06-06 1998-09-01 Hall; Frederick L. Artificial skin prepared from coclagen matrix containing transforming growth factor-β having a collagen binding site
US5935824A (en) 1996-01-31 1999-08-10 Technologene, Inc. Protein expression system
JP3945822B2 (ja) 1996-05-24 2007-07-18 カイロン コーポレイション 複数エピトープ融合タンパク質
CA2263784A1 (en) * 1998-03-23 1999-09-23 Megabios Corporation Dual-tagged proteins and their uses
US6420157B1 (en) * 1999-04-30 2002-07-16 Ortho-Mcneil Pharmaceutical, Inc. Zymogen activation system
FR2793492A1 (fr) 1999-05-11 2000-11-17 Pasteur Institut Nouveau procede de purification par affinite
US6482254B1 (en) * 2000-11-14 2002-11-19 E. I. Du Pont De Nemours And Company Deaerator insert

Also Published As

Publication number Publication date
AU774216B2 (en) 2004-06-17
US20020045193A1 (en) 2002-04-18
JP2003511078A (ja) 2003-03-25
US6379903B1 (en) 2002-04-30
EP1220933A1 (de) 2002-07-10
ES2321058T3 (es) 2009-06-02
WO2001027293A1 (en) 2001-04-19
ES2321058T7 (es) 2014-04-07
AU7704900A (en) 2001-04-23
DE60041345D1 (de) 2009-02-26
US7094548B2 (en) 2006-08-22
EP2075336B1 (de) 2016-01-06
EP2075336A1 (de) 2009-07-01
CA2386471A1 (en) 2001-04-19
US20060088878A1 (en) 2006-04-27
EP1220933B3 (de) 2013-12-11
CA2386471C (en) 2011-06-21
EP1220933B1 (de) 2009-01-07
JP4405125B2 (ja) 2010-01-27

Similar Documents

Publication Publication Date Title
ATE420186T1 (de) Verfahren zur reinigung von an mehreren epitopen fusionierten rekombinanten proteinen
DE60234131D1 (de) Neue fusionsproteine und molekulare bindungsassays
ATE447583T1 (de) Polypeptide und dafür kodierende nukleinsäure
ATE421977T1 (de) Reinigung von peptiden unter verwendung von metall-ion-affinitätschromatographie
DK0754704T3 (da) Syntetiske antigener til påvisning af antistoffer mod hepatitis c-virus
DE59109271D1 (de) HCV-spezifische Peptide, Mittel dazu und ihre Verwendung
ATE135403T1 (de) Herstellung und reinigung eines proteins, das mit einem bindungsprotein fusioniert ist
DK1027437T3 (da) Anvendelse af det WNT-1 inducerede udskilte polypeptid WISP-1
ATE301716T1 (de) Vmp-ähnliche sequenzen von pathogener borrelia
DE69827810D1 (de) Methode zum durchforsten von banken mittels chimären bindungspetiden
ATE326984T1 (de) Verfahren zur verbindung von molekularen substanzen
DE69333382D1 (de) Hyaluronan rezeptor (rezeptor durch hyaluronan verurzachte bewelichkeit) und hyaluronan bindende peptide
DE69533777D1 (de) Mit retenoid x-rezeptor interagierende polypeptide und verwandte moleküle und verfahren
FI930626A0 (fi) Proteinstruktur hos vaexttoxingelonin
EP0961547A4 (de) Neutralisationsempfindliche epitrope von cryptosporidiumparvum
DE69932834D1 (de) Chi-Conotoxinpeptide als Inhibitoren neuronaler Amintransporter
ATE143669T1 (de) Hcg-peptide zur verwendung in antikörper- reinigungsverfahren
SE9704141D0 (sv) New protein and nucleotide sequence, encoding said protein
WO2003070768A3 (en) Cd40 splice variants,method of detection thereof, compositions comprising said variant(s) or nucleic acid encoding the same and use thereof
ATE350398T1 (de) Protein s polypeptide und deren verwendungen
WO2005000883A8 (en) Polypeptides having binding affinity for insulin
ATE351909T1 (de) Neue peptidfragmente zur reinigung von proteinen
DE60207157D1 (de) Reinigungsverfahren auf Grundlage der Bindung von Enzymen and markierte Peptide
SE0003573D0 (sv) Method and means for producing novel von Willebrand factor binding proteins and their use in biotechnology
ATE301674T1 (de) Verfahren zur trennung und bestimmung von hydrophoben proteinen durch dünnschichtchromatographie

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties