ATE276371T1 - Escherichia coli wirt/vektorsystem basierend auf einer antibiotikafreien selektion durch komplementation einer auxotrophie - Google Patents
Escherichia coli wirt/vektorsystem basierend auf einer antibiotikafreien selektion durch komplementation einer auxotrophieInfo
- Publication number
- ATE276371T1 ATE276371T1 AT99113182T AT99113182T ATE276371T1 AT E276371 T1 ATE276371 T1 AT E276371T1 AT 99113182 T AT99113182 T AT 99113182T AT 99113182 T AT99113182 T AT 99113182T AT E276371 T1 ATE276371 T1 AT E276371T1
- Authority
- AT
- Austria
- Prior art keywords
- auxotrophy
- complementation
- antibiotic
- system based
- escherichia coli
- Prior art date
Links
- 241000588724 Escherichia coli Species 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 241000894006 Bacteria Species 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 239000013604 expression vector Substances 0.000 abstract 1
- 239000003550 marker Substances 0.000 abstract 1
- 230000009465 prokaryotic expression Effects 0.000 abstract 1
- 230000010076 replication Effects 0.000 abstract 1
- 230000004083 survival effect Effects 0.000 abstract 1
- 238000003786 synthesis reaction Methods 0.000 abstract 1
- 230000035897 transcription Effects 0.000 abstract 1
- 238000013518 transcription Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/555—Interferons [IFN]
- C07K14/56—IFN-alpha
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medical Treatment And Welfare Office Work (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP98113156 | 1998-07-15 | ||
| EP98119078 | 1998-10-09 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE276371T1 true ATE276371T1 (de) | 2004-10-15 |
Family
ID=26149433
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT99113182T ATE276371T1 (de) | 1998-07-15 | 1999-07-08 | Escherichia coli wirt/vektorsystem basierend auf einer antibiotikafreien selektion durch komplementation einer auxotrophie |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US6291245B1 (de) |
| JP (1) | JP3801389B2 (de) |
| KR (1) | KR100355963B1 (de) |
| CN (1) | CN1196792C (de) |
| AT (1) | ATE276371T1 (de) |
| AU (1) | AU720627B2 (de) |
| CA (1) | CA2274982C (de) |
| DE (1) | DE69920113T2 (de) |
| ES (1) | ES2227938T3 (de) |
Families Citing this family (50)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1322138C (zh) * | 2000-06-23 | 2007-06-20 | 诺维信公司 | 用于基因的稳定的染色体多拷贝整合的方法 |
| JP2002136288A (ja) * | 2000-11-06 | 2002-05-14 | National Cancer Center-Japan | 酵母を用いた真核生物遺伝子の機能推定方法 |
| SE0102204D0 (sv) * | 2001-06-21 | 2001-06-21 | Leif Isaksson | New method |
| US7270827B2 (en) * | 2001-10-26 | 2007-09-18 | University Of Tennessee Research Foundation | Multivalent streptococcal vaccine compositions and methods for use |
| DE10214406A1 (de) * | 2002-03-30 | 2003-10-09 | Nutrinova Gmbh | Verfahren und Marker zur einfachen Transformation und Selektion von rekombinanten Protisten |
| CA2443365C (en) * | 2002-11-19 | 2010-01-12 | F. Hoffmann-La Roche Ag | Methods for the recombinant production of antifusogenic peptides |
| EP1466974A1 (de) * | 2003-04-11 | 2004-10-13 | Max-Delbrück-Centrum Für Molekulare Medizin | Induzierbare ortsspezifische Mutagenese durch bedingte Gen-rettung |
| DE60302776T2 (de) * | 2003-06-12 | 2006-08-17 | F. Hoffmann-La Roche Ag | Verfahren zur rekombinanten Herstellung von Polypeptiden |
| KR101056983B1 (ko) * | 2003-07-11 | 2011-08-16 | 가부시키가이샤 구라레 | 적층 시트와 그 설계방법 및 배면 투사형 스크린과 그제조방법 |
| FI116068B (fi) * | 2003-09-15 | 2005-09-15 | Fit Biotech Oyj Plc | Uusi selektiojärjestelmä, siinä käyttökelpoinen vektori, bakteerisolukantoja sekä menetelmä solujen valikoimiseksi |
| WO2005052151A1 (en) * | 2003-11-19 | 2005-06-09 | Dow Global Technologies Inc. | Improved protein expression systems |
| DE10360483B4 (de) * | 2003-12-22 | 2007-11-15 | Scil Proteins Gmbh | Expressionsvektor und dessen Verwendung |
| CA2608579C (en) | 2005-05-26 | 2019-09-10 | Cytos Biotechnology Ag | Scalable fermentation process |
| CA2624923C (en) | 2005-10-06 | 2016-06-07 | Franck Martin | Novel transformation selection system comprising pyrc gene complementation |
| EP2049668A2 (de) | 2006-08-03 | 2009-04-22 | Wisconsin Alumni Research Foundation | Vektorensysteme |
| AU2010247938B2 (en) | 2009-05-11 | 2016-02-18 | Pelican Technology Holdings, Inc. | Production of recombinant proteins utilizing non-antibiotic selection methods and the incorporation of non-natural amino acids therein |
| CN103124791B (zh) | 2010-06-01 | 2016-08-24 | 帝斯曼知识产权资产管理有限公司 | 从细胞中提取脂质以及由此获得的产品 |
| RU2013111677A (ru) | 2010-08-30 | 2014-10-10 | Ф. Хоффманн-Ля Рош Аг | Конструкция для экспрессии у прокариот |
| MX2013001539A (es) | 2010-08-30 | 2013-03-18 | Hoffmann La Roche | Metodo para producir una particula de tetranectina-apolipoproteina a-1, la particula lipidica obtenida por el mismoy su uso. |
| WO2012028524A2 (en) | 2010-08-30 | 2012-03-08 | F. Hoffmann-La Roche Ag | Method for producing a lipid particle, the lipid particle itself and its use |
| RU2013112562A (ru) | 2010-08-30 | 2014-10-10 | Ф. Хоффманн-Ля Рош Аг | Тетранектин-аполипопротеин а-i, содержащие его липидные частицы и его применение |
| RU2631001C2 (ru) * | 2010-08-30 | 2017-09-15 | Ф.Хоффманн-Ля Рош Аг | Способ получения полипептида |
| MX2013002556A (es) | 2010-09-14 | 2013-05-28 | Hoffmann La Roche | Polipeptido de fusion de dedo de serpina. |
| WO2012146630A1 (en) | 2011-04-29 | 2012-11-01 | F. Hoffmann-La Roche Ag | N-terminal acylated polypeptides, methods for their production and uses thereof |
| EP2748191A1 (de) | 2011-08-25 | 2014-07-02 | F.Hoffmann-La Roche Ag | Verkürztes tetranectin-apolipoprotein-a-i-fusionsprotein, lipidpartikel damit und verwendungen davon |
| RU2014137621A (ru) | 2012-02-29 | 2016-04-20 | Ф.Хоффманн-Ля Рош Аг | Способ уменьшения 1->2 сдвига рамки считывания |
| CA2863108A1 (en) | 2012-02-29 | 2013-09-06 | F. Hoffmann-La Roche Ag | Method for reduction of 1->3 reading frame shifts |
| RU2014137623A (ru) | 2012-02-29 | 2016-04-20 | Ф. Хоффманн-Ля Рош Аг | Колоночное ферментативное расщепление |
| EP2831100A4 (de) | 2012-03-26 | 2016-02-10 | Pronutria Inc | Nährstoffproteine und verfahren |
| RU2634407C2 (ru) | 2012-03-26 | 2017-10-26 | Эксселла Хелт Инк. | Фрагменты пищевых белков и способы их применения |
| EP2831098A4 (de) | 2012-03-26 | 2016-12-07 | Axcella Health Inc | Geladene nährstoffproteine und verfahren |
| US9700071B2 (en) | 2012-03-26 | 2017-07-11 | Axcella Health Inc. | Nutritive fragments, proteins and methods |
| JP6224077B2 (ja) | 2012-04-17 | 2017-11-01 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 改変された核酸を用いてポリペプチドを発現させるための方法 |
| MA38165A1 (fr) | 2012-11-08 | 2018-07-31 | Hoffmann La Roche | Protéines de liaison à l'antigène her3 se liant à l'épingle à cheveux beta de her3 |
| SI2959006T1 (sl) | 2013-02-22 | 2018-10-30 | F. Hoffmann-La Roche Ag | Uporaba prokariontskega seva, ozdravljenega aminokislinske avksotrofije, za proizvodnjo rekombinantnega polipeptida |
| RU2016110800A (ru) | 2013-09-25 | 2017-10-30 | Пронутриа Биосайенсис, Инк. | Составы и композиции для поддержания и увеличения мышечной массы, силы и результативности, и способы их производства и использования |
| WO2015051199A2 (en) | 2013-10-06 | 2015-04-09 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Modified pseudomonas exotoxin a |
| US10316322B2 (en) | 2014-07-02 | 2019-06-11 | Sutro Biopharma, Inc. | High growth capacity auxotrophic Escherichia coli and methods of use |
| EP3184547A1 (de) | 2015-10-29 | 2017-06-28 | F. Hoffmann-La Roche AG | Anti-tpbg-antikörper und verfahren zur verwendung |
| CN108463546B (zh) * | 2015-12-11 | 2022-03-11 | 瓦克化学股份公司 | 用于无抗生素发酵制备低分子量物质和蛋白质的微生物菌株和方法 |
| EP3957645A1 (de) * | 2016-12-20 | 2022-02-23 | Universität Basel | Virulenzabgeschwächte bakterienbasierte proteinabgabe |
| WO2019018270A1 (en) * | 2017-07-21 | 2019-01-24 | Conagen, Inc. | PLASMID DEPENDENCE SYSTEM FOR DIRECTING THE EXPRESSION OF A DESIRED GENE |
| DE18305781T1 (de) | 2018-06-20 | 2020-09-17 | Eligo Bioscience | Bakterielles freisetzungsvehikel, verfahren zur herstellung und verwendungen davon |
| JP6583759B1 (ja) * | 2018-11-22 | 2019-10-02 | 勝 枦 | Ｄｎａを改変したり、新たに核酸を導入した細胞を抗生物質を利用せずに確認、選抜する方法 |
| CN111635879B (zh) * | 2019-03-01 | 2022-04-22 | 中国科学院天津工业生物技术研究所 | L-赖氨酸高产菌株及其构建方法和应用 |
| CN114846024A (zh) | 2019-12-20 | 2022-08-02 | 豪夫迈·罗氏有限公司 | Il-37融合蛋白及其用途 |
| US12098372B2 (en) | 2019-12-30 | 2024-09-24 | Eligo Bioscience | Microbiome modulation of a host by delivery of DNA payloads with minimized spread |
| US11746352B2 (en) | 2019-12-30 | 2023-09-05 | Eligo Bioscience | Microbiome modulation of a host by delivery of DNA payloads with minimized spread |
| WO2022144381A1 (en) | 2020-12-30 | 2022-07-07 | Eligo Bioscience | Microbiome modulation of a host by delivery of dna payloads with minimized spread |
| US11739304B2 (en) | 2021-05-12 | 2023-08-29 | Eligo Bioscience | Production of lytic phages |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2511032B1 (fr) | 1981-08-07 | 1985-08-09 | Centre Nat Rech Scient | Nouveaux plasmides et souches bacteriennes transformees et leur application a la synthese de lysine |
| US4689406A (en) * | 1983-08-10 | 1987-08-25 | Amgen | Enhancement of microbial expression of polypeptides |
| NL8400687A (nl) | 1984-03-02 | 1985-10-01 | Unilever Nv | Recombinant plasmiden; recombinant plasmiden bevattende bacterien; werkwijze voor het bereiden of vervaardigen van een zuivelproduct; werkwijze voor het bereiden van een eiwit. |
| EP0178764A1 (de) | 1984-08-28 | 1986-04-23 | Genex Corporation | Verfahren zur Stabilisierung eines Wirtsmikroorganismus-Expressionsvektor-Systems für die Herstellung von Proteinen in grossem Umfang |
| DK594084A (da) | 1984-12-12 | 1986-06-13 | Novo Industri As | Fremgangsmaade til stabilisering af extra-chromosomale elementer i bakterier under dyrkning |
| US5198343A (en) | 1986-08-05 | 1993-03-30 | Transgene, S.A. | Method for expressing a heterologous protein in a dapD- mutant of E. coli and the strain obtained |
| CA1340903C (fr) | 1986-08-05 | 2000-02-22 | Transgene S.A. | Procede de stabilisation d'un plasmide contenu dans une soche bacterienne et souche obtenue |
| DE3853683T2 (de) | 1987-10-07 | 1995-08-31 | Univ Washington | Verfahren zur erhaltung eines erwünschten rekombinanten gens in einer genetischen zellpopulation. |
| AU643752B2 (en) | 1990-01-31 | 1993-11-25 | Upjohn Company, The | Strain RAL-4, useful for production of small proteins and peptides |
| JPH05505308A (ja) | 1990-03-13 | 1993-08-12 | ハワイ・バイオテクノロジー・グループ・インコーポレイテツド | アオパンカビ発現システム |
| GB9215541D0 (en) | 1992-07-22 | 1992-09-02 | Celltech Ltd | Protein expression system |
| US5677170A (en) * | 1994-03-02 | 1997-10-14 | The Johns Hopkins University | In vitro transposition of artificial transposons |
-
1999
- 1999-06-25 US US09/344,888 patent/US6291245B1/en not_active Expired - Lifetime
- 1999-07-08 AT AT99113182T patent/ATE276371T1/de not_active IP Right Cessation
- 1999-07-08 ES ES99113182T patent/ES2227938T3/es not_active Expired - Lifetime
- 1999-07-08 DE DE69920113T patent/DE69920113T2/de not_active Expired - Lifetime
- 1999-07-13 CA CA002274982A patent/CA2274982C/en not_active Expired - Lifetime
- 1999-07-14 AU AU40115/99A patent/AU720627B2/en not_active Expired
- 1999-07-14 KR KR1019990028382A patent/KR100355963B1/ko not_active Expired - Lifetime
- 1999-07-15 JP JP20230399A patent/JP3801389B2/ja not_active Expired - Lifetime
- 1999-07-15 CN CNB991103718A patent/CN1196792C/zh not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| CA2274982C (en) | 2005-01-04 |
| AU720627B2 (en) | 2000-06-08 |
| KR20000011710A (ko) | 2000-02-25 |
| US6291245B1 (en) | 2001-09-18 |
| CN1241635A (zh) | 2000-01-19 |
| ES2227938T3 (es) | 2005-04-01 |
| JP2000050888A (ja) | 2000-02-22 |
| AU4011599A (en) | 2000-02-10 |
| DE69920113T2 (de) | 2005-12-15 |
| CA2274982A1 (en) | 2000-01-15 |
| JP3801389B2 (ja) | 2006-07-26 |
| KR100355963B1 (ko) | 2002-10-19 |
| DE69920113D1 (de) | 2004-10-21 |
| CN1196792C (zh) | 2005-04-13 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |