ATE279427T1 - Verfahren zur immobilisierung von nukleinsäure- molekülen - Google Patents

Verfahren zur immobilisierung von nukleinsäure- molekülen

Info

Publication number
ATE279427T1
ATE279427T1 AT95904282T AT95904282T ATE279427T1 AT E279427 T1 ATE279427 T1 AT E279427T1 AT 95904282 T AT95904282 T AT 95904282T AT 95904282 T AT95904282 T AT 95904282T AT E279427 T1 ATE279427 T1 AT E279427T1
Authority
AT
Austria
Prior art keywords
nucleic acid
support
detergent
salt
concentration
Prior art date
Application number
AT95904282T
Other languages
English (en)
Inventor
Theo Nikiforov
Michael R Knapp
Original Assignee
Beckman Coulter Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Beckman Coulter Inc filed Critical Beckman Coulter Inc
Application granted granted Critical
Publication of ATE279427T1 publication Critical patent/ATE279427T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT95904282T 1993-12-06 1994-12-06 Verfahren zur immobilisierung von nukleinsäure- molekülen ATE279427T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US16239793A 1993-12-06 1993-12-06
US08/341,148 US5610287A (en) 1993-12-06 1994-11-16 Method for immobilizing nucleic acid molecules
PCT/US1994/014096 WO1995015970A1 (en) 1993-12-06 1994-12-06 Method for the immobilization of nucleic acid molecules

Publications (1)

Publication Number Publication Date
ATE279427T1 true ATE279427T1 (de) 2004-10-15

Family

ID=26858720

Family Applications (1)

Application Number Title Priority Date Filing Date
AT95904282T ATE279427T1 (de) 1993-12-06 1994-12-06 Verfahren zur immobilisierung von nukleinsäure- molekülen

Country Status (7)

Country Link
US (1) US5610287A (de)
EP (1) EP0684952B1 (de)
AT (1) ATE279427T1 (de)
AU (1) AU682741B2 (de)
CA (1) CA2155634A1 (de)
DE (1) DE69434066T2 (de)
WO (1) WO1995015970A1 (de)

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EP0684952B1 (de) 2004-10-13
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US5610287A (en) 1997-03-11
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