ATE298090T1 - Verfahren zur selektion von zellen die antikörper exprimieren - Google Patents

Verfahren zur selektion von zellen die antikörper exprimieren

Info

Publication number
ATE298090T1
ATE298090T1 AT02762376T AT02762376T ATE298090T1 AT E298090 T1 ATE298090 T1 AT E298090T1 AT 02762376 T AT02762376 T AT 02762376T AT 02762376 T AT02762376 T AT 02762376T AT E298090 T1 ATE298090 T1 AT E298090T1
Authority
AT
Austria
Prior art keywords
selecting cells
express antibodies
antibody
protein
cells
Prior art date
Application number
AT02762376T
Other languages
English (en)
Inventor
Andy Racher
Rabinder Singh
Original Assignee
Lonza Group Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lonza Group Ag filed Critical Lonza Group Ag
Application granted granted Critical
Publication of ATE298090T1 publication Critical patent/ATE298090T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/16—Animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
    • A61K39/44—Antibodies bound to carriers

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Food Science & Technology (AREA)
  • Wood Science & Technology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Engineering & Computer Science (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
AT02762376T 2001-07-27 2002-07-19 Verfahren zur selektion von zellen die antikörper exprimieren ATE298090T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0118337.5A GB0118337D0 (en) 2001-07-27 2001-07-27 Method for selecting antibody expressing cells
PCT/EP2002/008054 WO2003012449A2 (en) 2001-07-27 2002-07-19 Method for selecting antibody expressing cells

Publications (1)

Publication Number Publication Date
ATE298090T1 true ATE298090T1 (de) 2005-07-15

Family

ID=9919307

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02762376T ATE298090T1 (de) 2001-07-27 2002-07-19 Verfahren zur selektion von zellen die antikörper exprimieren

Country Status (12)

Country Link
US (1) US20040219611A1 (de)
EP (1) EP1415158B1 (de)
JP (1) JP4202251B2 (de)
KR (1) KR100663687B1 (de)
CN (1) CN1296710C (de)
AT (1) ATE298090T1 (de)
CA (1) CA2454088A1 (de)
DE (1) DE60204699T2 (de)
ES (1) ES2243757T3 (de)
GB (1) GB0118337D0 (de)
PT (1) PT1415158E (de)
WO (1) WO2003012449A2 (de)

Families Citing this family (29)

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Publication number Priority date Publication date Assignee Title
JP2005526517A (ja) * 2002-05-22 2005-09-08 バイオジェン・アイデック・エムエイ・インコーポレイテッド 分泌性ポリペプチドの検出
US20040067532A1 (en) 2002-08-12 2004-04-08 Genetastix Corporation High throughput generation and affinity maturation of humanized antibody
ES2374068T3 (es) * 2002-12-03 2012-02-13 Ucb Pharma, S.A. Ensayo para identificar células productoras de anticuerpos.
ES2385829T3 (es) 2003-08-20 2012-08-01 Ucb Pharma, S.A. Métodos para obtener anticuerpos
WO2006052351A2 (en) * 2004-10-08 2006-05-18 Cedars-Sinai Medical Center Biological microbeads for various flow cytometric applications
EP1910513B1 (de) * 2005-07-01 2016-08-10 John Schrader Verfahren zur Isolierung Antikörper-sezernierender Zellen aus dem Blut immunisierter Tiere und Menschen und Erzeugung monoklonaler Antikörper aus diesen Antikörper-sezernierenden Zellen.
EP2617827A1 (de) 2007-03-26 2013-07-24 Celexion, LLC Zelloberflächenanzeige, Abtastung und Herstellung von Proteinen von Interesse
US12529164B2 (en) 2007-09-14 2026-01-20 Adimab, Llc Rationally designed, synthetic antibody libraries and uses therefor
US8877688B2 (en) 2007-09-14 2014-11-04 Adimab, Llc Rationally designed, synthetic antibody libraries and uses therefor
EP3124497B1 (de) 2007-09-14 2020-04-15 Adimab, LLC Synthetische antikörperbibliotheken mit rationalem design und verwendungen davon
JPWO2009044650A1 (ja) * 2007-10-05 2011-02-03 株式会社カネカ 免疫グロブリンおよび／または免疫グロブリン複合体に結合活性を有する、標識されたペプチド、および上記ペプチドを用いた免疫グロブリンの検出方法または測定方法
US8637435B2 (en) * 2007-11-16 2014-01-28 Merck Sharp & Dohme Corp. Eukaryotic cell display systems
JP5731827B2 (ja) 2008-03-03 2015-06-10 グライコフィ， インコーポレイテッド 下等真核生物中での組換えタンパク質の表面ディスプレイ
US8067339B2 (en) 2008-07-09 2011-11-29 Merck Sharp & Dohme Corp. Surface display of whole antibodies in eukaryotes
EP2390661B1 (de) * 2010-05-02 2015-06-24 Miltenyi Biotec GmbH Verankerungs-/Erfassungsmittel zum Auswählen oder Analysieren einer CHO-Zelle entsprechend einem Produkt, das von der CHO-Zelle sezerniert wird
KR102318383B1 (ko) 2010-07-16 2021-10-27 아디맵 엘엘씨 항체 라이브러리
CN103185803A (zh) 2011-12-31 2013-07-03 深圳迈瑞生物医疗电子股份有限公司 一种鉴定抗体敏感性和克隆细胞株的方法及试剂盒
US8921055B2 (en) * 2012-10-30 2014-12-30 Berkeley Lights, Inc. Detecting cells secreting a protein of interest
EP2925867B1 (de) 2012-11-30 2018-06-27 Larix Biosciences LLC Neuartiges screening-verfahren für zelllinien
DE112014006603A5 (de) 2014-04-20 2017-01-19 new/era/mabs GmbH Biomoleküle freisetzende Zelle und deren Selektion mittels eines Oberflächenproteins sowie für dieses codierende Nukleinsäure, deren Expressionsvektor und dessen Empfängerzelle
WO2015184626A1 (en) * 2014-06-05 2015-12-10 Wuhan Yzy Biopharma Co., Ltd Preparation and selection of cells for producing bispecific antibodies
CN104833625B (zh) * 2015-06-04 2018-02-23 武汉友芝友生物制药有限公司 表达重组抗体cho细胞系稳定性的早期鉴定方法
US9395367B1 (en) 2016-02-29 2016-07-19 Rarecyte, Inc. Method to identify antigen-specific B cells for antibody development
US10222373B2 (en) 2016-02-29 2019-03-05 Rarecyte, Inc. Method to identify antigen-specific immune cells
US9442113B1 (en) 2016-02-29 2016-09-13 Rarecyte, Inc. Method to identify antigen-specific immune cells for therapeutic development
JP7607573B2 (ja) * 2019-09-30 2024-12-27 東京応化工業株式会社 分泌物産生細胞のスクリーニング方法、及び分泌物産生細胞のスクリーニングキット
CN114441752B (zh) * 2020-11-03 2024-11-26 中国科学院苏州纳米技术与纳米仿生研究所 一种筛选方法
CN112710833B (zh) * 2021-01-13 2023-01-31 上海交通大学 基于微管流控芯片的细胞捕获方法
CN117015710A (zh) * 2021-03-10 2023-11-07 索尼集团公司 生物粒子分析方法及用于生物粒子分析的试剂盒

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5028524A (en) * 1987-04-24 1991-07-02 Takeda Chemical Industries, Ltd. Assay for anti-pre-S antibody
EP0805982A1 (de) * 1995-01-25 1997-11-12 Murex Diagnostics Corporation Verbesserungen für immunoassays
AU765085B2 (en) * 1998-05-11 2003-09-11 Miltenyi Biotec Gmbh Method of direct selection of antigen-specific T cells
JP4022005B2 (ja) * 1998-10-22 2007-12-12 わかもと製薬株式会社 簡易抗体検査方法及び検査用キット
JP2001059845A (ja) * 1999-08-24 2001-03-06 Fuji Photo Film Co Ltd 乾式分析方法及び乾式分析要素
GB0002539D0 (en) * 2000-02-03 2000-03-29 Lonza Biologics Plc Process for the discrimination of immunoglobulin G-producing cells from non-immunoglobulin G-producing cells
US6919183B2 (en) * 2001-01-16 2005-07-19 Regeneron Pharmaceuticals, Inc. Isolating cells expressing secreted proteins

Also Published As

Publication number Publication date
DE60204699D1 (de) 2005-07-21
EP1415158A2 (de) 2004-05-06
JP4202251B2 (ja) 2008-12-24
US20040219611A1 (en) 2004-11-04
ES2243757T3 (es) 2005-12-01
WO2003012449A3 (en) 2003-10-23
KR100663687B1 (ko) 2007-01-02
GB0118337D0 (en) 2001-09-19
KR20040021660A (ko) 2004-03-10
JP2005526950A (ja) 2005-09-08
CN1296710C (zh) 2007-01-24
DE60204699T2 (de) 2006-05-11
PT1415158E (pt) 2005-10-31
CN1545621A (zh) 2004-11-10
CA2454088A1 (en) 2003-02-13
EP1415158B1 (de) 2005-06-15
WO2003012449A2 (en) 2003-02-13

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