ATE298090T1 - Verfahren zur selektion von zellen die antikörper exprimieren - Google Patents

Verfahren zur selektion von zellen die antikörper exprimieren

Info

Publication number
ATE298090T1
ATE298090T1 AT02762376T AT02762376T ATE298090T1 AT E298090 T1 ATE298090 T1 AT E298090T1 AT 02762376 T AT02762376 T AT 02762376T AT 02762376 T AT02762376 T AT 02762376T AT E298090 T1 ATE298090 T1 AT E298090T1
Authority
AT
Austria
Prior art keywords
selecting cells
express antibodies
antibody
protein
cells
Prior art date
Application number
AT02762376T
Other languages
English (en)
Inventor
Andy Racher
Rabinder Singh
Original Assignee
Lonza Group Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lonza Group Ag filed Critical Lonza Group Ag
Application granted granted Critical
Publication of ATE298090T1 publication Critical patent/ATE298090T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/16—Animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
    • A61K39/44—Antibodies bound to carriers

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Food Science & Technology (AREA)
  • Wood Science & Technology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Engineering & Computer Science (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
AT02762376T 2001-07-27 2002-07-19 Verfahren zur selektion von zellen die antikörper exprimieren ATE298090T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0118337.5A GB0118337D0 (en) 2001-07-27 2001-07-27 Method for selecting antibody expressing cells
PCT/EP2002/008054 WO2003012449A2 (en) 2001-07-27 2002-07-19 Method for selecting antibody expressing cells

Publications (1)

Publication Number Publication Date
ATE298090T1 true ATE298090T1 (de) 2005-07-15

Family

ID=9919307

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02762376T ATE298090T1 (de) 2001-07-27 2002-07-19 Verfahren zur selektion von zellen die antikörper exprimieren

Country Status (12)

Country Link
US (1) US20040219611A1 (de)
EP (1) EP1415158B1 (de)
JP (1) JP4202251B2 (de)
KR (1) KR100663687B1 (de)
CN (1) CN1296710C (de)
AT (1) ATE298090T1 (de)
CA (1) CA2454088A1 (de)
DE (1) DE60204699T2 (de)
ES (1) ES2243757T3 (de)
GB (1) GB0118337D0 (de)
PT (1) PT1415158E (de)
WO (1) WO2003012449A2 (de)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1668634A (zh) * 2002-05-22 2005-09-14 比奥根艾迪克Ma公司 分泌性多肽的检测
US20040067532A1 (en) 2002-08-12 2004-04-08 Genetastix Corporation High throughput generation and affinity maturation of humanized antibody
WO2004051268A1 (en) * 2002-12-03 2004-06-17 Celltech R & D Limited Assay for identifying antibody producing cells
ES2385829T3 (es) * 2003-08-20 2012-08-01 Ucb Pharma, S.A. Métodos para obtener anticuerpos
EP1797436A2 (de) * 2004-10-08 2007-06-20 Cedars-Sinai Medical Center Biologische mikrokugeln für verschiedene durchflusszytometrische anwendungen
EP2343363A1 (de) * 2005-07-01 2011-07-13 John Schrader Verfahren zur Isolierung von Zellen und Erzeugung monoklonaler Antikörper unter Verwendung einer Einzel-Epitop Mehrfach-Färbung
EP2617827A1 (de) 2007-03-26 2013-07-24 Celexion, LLC Zelloberflächenanzeige, Abtastung und Herstellung von Proteinen von Interesse
US12529164B2 (en) 2007-09-14 2026-01-20 Adimab, Llc Rationally designed, synthetic antibody libraries and uses therefor
WO2009036379A2 (en) 2007-09-14 2009-03-19 Adimab, Inc. Rationally designed, synthetic antibody libraries and uses therefor
US8877688B2 (en) 2007-09-14 2014-11-04 Adimab, Llc Rationally designed, synthetic antibody libraries and uses therefor
US20100255606A1 (en) * 2007-10-05 2010-10-07 Nobuhiko Sato Labeled peptide having activity of binding to immunoglobulin and/or immunoglobulin complex and method of detecting or assaying immunoglobulin by using the peptide
US8637435B2 (en) * 2007-11-16 2014-01-28 Merck Sharp & Dohme Corp. Eukaryotic cell display systems
CA2715212A1 (en) 2008-03-03 2009-09-11 Glycofi, Inc. Surface display of recombinant proteins in lower eukaryotes
US8067339B2 (en) * 2008-07-09 2011-11-29 Merck Sharp & Dohme Corp. Surface display of whole antibodies in eukaryotes
EP2390661B1 (de) * 2010-05-02 2015-06-24 Miltenyi Biotec GmbH Verankerungs-/Erfassungsmittel zum Auswählen oder Analysieren einer CHO-Zelle entsprechend einem Produkt, das von der CHO-Zelle sezerniert wird
MX381912B (es) 2010-07-16 2025-03-13 Adimab Llc Colecciones de anticuerpos.
CN103185803A (zh) 2011-12-31 2013-07-03 深圳迈瑞生物医疗电子股份有限公司 一种鉴定抗体敏感性和克隆细胞株的方法及试剂盒
US8921055B2 (en) * 2012-10-30 2014-12-30 Berkeley Lights, Inc. Detecting cells secreting a protein of interest
EP3382026B1 (de) 2012-11-30 2021-01-06 Larix Biosciences LLC Neuartiges screening-verfahren für zelllinien
WO2015161835A1 (de) * 2014-04-20 2015-10-29 New / Era / Mabs Gmbh Biomoleküle freisetzende zelle und deren selektion mittels eines oberflächenproteins
WO2015184626A1 (en) * 2014-06-05 2015-12-10 Wuhan Yzy Biopharma Co., Ltd Preparation and selection of cells for producing bispecific antibodies
CN104833625B (zh) * 2015-06-04 2018-02-23 武汉友芝友生物制药有限公司 表达重组抗体cho细胞系稳定性的早期鉴定方法
US10222373B2 (en) 2016-02-29 2019-03-05 Rarecyte, Inc. Method to identify antigen-specific immune cells
US9395367B1 (en) 2016-02-29 2016-07-19 Rarecyte, Inc. Method to identify antigen-specific B cells for antibody development
US9442113B1 (en) 2016-02-29 2016-09-13 Rarecyte, Inc. Method to identify antigen-specific immune cells for therapeutic development
KR20220070220A (ko) * 2019-09-30 2022-05-30 도오꾜오까고오교 가부시끼가이샤 분비물 산생 세포의 스크리닝 방법, 및 분비물 산생 세포의 스크리닝 키트
CN114441752B (zh) * 2020-11-03 2024-11-26 中国科学院苏州纳米技术与纳米仿生研究所 一种筛选方法
CN112710833B (zh) * 2021-01-13 2023-01-31 上海交通大学 基于微管流控芯片的细胞捕获方法
US20240310375A1 (en) * 2021-03-10 2024-09-19 Sony Group Corporation Bioparticle analysis method and reagent kit for bioparticle analysis

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5028524A (en) * 1987-04-24 1991-07-02 Takeda Chemical Industries, Ltd. Assay for anti-pre-S antibody
EP0805982A1 (de) * 1995-01-25 1997-11-12 Murex Diagnostics Corporation Verbesserungen für immunoassays
CA2330678C (en) * 1998-05-11 2009-04-07 Miltenyi Biotec Gmbh Method of direct selection of antigen-specific t cells
JP4022005B2 (ja) * 1998-10-22 2007-12-12 わかもと製薬株式会社 簡易抗体検査方法及び検査用キット
JP2001059845A (ja) * 1999-08-24 2001-03-06 Fuji Photo Film Co Ltd 乾式分析方法及び乾式分析要素
GB0002539D0 (en) * 2000-02-03 2000-03-29 Lonza Biologics Plc Process for the discrimination of immunoglobulin G-producing cells from non-immunoglobulin G-producing cells
ATE325865T1 (de) * 2001-01-16 2006-06-15 Regeneron Pharma Isolierung von sezernierte proteine exprimierenden zellen

Also Published As

Publication number Publication date
KR20040021660A (ko) 2004-03-10
KR100663687B1 (ko) 2007-01-02
CN1545621A (zh) 2004-11-10
JP4202251B2 (ja) 2008-12-24
WO2003012449A3 (en) 2003-10-23
EP1415158A2 (de) 2004-05-06
JP2005526950A (ja) 2005-09-08
DE60204699T2 (de) 2006-05-11
CA2454088A1 (en) 2003-02-13
CN1296710C (zh) 2007-01-24
WO2003012449A2 (en) 2003-02-13
GB0118337D0 (en) 2001-09-19
PT1415158E (pt) 2005-10-31
ES2243757T3 (es) 2005-12-01
EP1415158B1 (de) 2005-06-15
US20040219611A1 (en) 2004-11-04
DE60204699D1 (de) 2005-07-21

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