ATE308042T1 - Vorrichtung und verfahren für die trennung von analyten - Google Patents
Vorrichtung und verfahren für die trennung von analytenInfo
- Publication number
- ATE308042T1 ATE308042T1 AT02772306T AT02772306T ATE308042T1 AT E308042 T1 ATE308042 T1 AT E308042T1 AT 02772306 T AT02772306 T AT 02772306T AT 02772306 T AT02772306 T AT 02772306T AT E308042 T1 ATE308042 T1 AT E308042T1
- Authority
- AT
- Austria
- Prior art keywords
- compartments
- compounds
- analyte solution
- separation
- solution
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 2
- 238000000926 separation method Methods 0.000 title abstract 2
- 150000001875 compounds Chemical class 0.000 abstract 4
- 239000012491 analyte Substances 0.000 abstract 3
- 230000003139 buffering effect Effects 0.000 abstract 2
- 230000007935 neutral effect Effects 0.000 abstract 2
- 239000000126 substance Substances 0.000 abstract 2
- 230000005684 electric field Effects 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44795—Isoelectric focusing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Electrochemistry (AREA)
- Pathology (AREA)
- Peptides Or Proteins (AREA)
- Electrostatic Separation (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Sampling And Sample Adjustment (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB0121189.5A GB0121189D0 (en) | 2001-08-31 | 2001-08-31 | Apparatus and method for separating an analyte |
| PCT/EP2002/010369 WO2003019172A2 (en) | 2001-08-31 | 2002-08-30 | Apparatus and method for separating an analyte |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE308042T1 true ATE308042T1 (de) | 2005-11-15 |
Family
ID=9921346
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT02772306T ATE308042T1 (de) | 2001-08-31 | 2002-08-30 | Vorrichtung und verfahren für die trennung von analyten |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US7390389B2 (de) |
| EP (1) | EP1421373B1 (de) |
| JP (1) | JP4227016B2 (de) |
| AT (1) | ATE308042T1 (de) |
| DE (1) | DE60206937T2 (de) |
| GB (1) | GB0121189D0 (de) |
| WO (1) | WO2003019172A2 (de) |
Families Citing this family (49)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6803568B2 (en) | 2001-09-19 | 2004-10-12 | Predicant Biosciences, Inc. | Multi-channel microfluidic chip for electrospray ionization |
| US7105810B2 (en) | 2001-12-21 | 2006-09-12 | Cornell Research Foundation, Inc. | Electrospray emitter for microfluidic channel |
| GB0226160D0 (en) * | 2002-11-08 | 2002-12-18 | Diagnoswiss Sa | Apparatus for dispensing a sample in electrospray mass spectrometers |
| GB0300820D0 (en) * | 2003-01-14 | 2003-02-12 | Diagnoswiss Sa | Membrane-microchannel strip |
| US7007710B2 (en) | 2003-04-21 | 2006-03-07 | Predicant Biosciences, Inc. | Microfluidic devices and methods |
| EP1673595A2 (de) * | 2003-09-15 | 2006-06-28 | DiagnoSwiss S.A. | Einrichtung zur überwachung einer mikrofluidischen strömung |
| JP2007524069A (ja) * | 2003-09-24 | 2007-08-23 | アジレント・テクノロジーズ・インク | フレームを使用した分子の抽出 |
| US7537807B2 (en) | 2003-09-26 | 2009-05-26 | Cornell University | Scanned source oriented nanofiber formation |
| US10337054B2 (en) | 2004-02-02 | 2019-07-02 | Quantum-Si Incorporated | Enrichment of nucleic acid targets |
| US8529744B2 (en) | 2004-02-02 | 2013-09-10 | Boreal Genomics Corp. | Enrichment of nucleic acid targets |
| WO2005072854A1 (en) | 2004-02-02 | 2005-08-11 | The University Of British Columbia | Scodaphoresis and methods and apparatus for moving and concentrating particles |
| US20060060769A1 (en) | 2004-09-21 | 2006-03-23 | Predicant Biosciences, Inc. | Electrospray apparatus with an integrated electrode |
| US7591883B2 (en) | 2004-09-27 | 2009-09-22 | Cornell Research Foundation, Inc. | Microfiber supported nanofiber membrane |
| US20070240986A1 (en) * | 2004-11-12 | 2007-10-18 | Diagnoswiss S.A. | Microfluidic Device with Minimized Ohmic Resistance |
| WO2006063625A1 (en) * | 2004-12-17 | 2006-06-22 | Agilent Technologies, Inc. | Fractionation using electro elution |
| CA2496294A1 (en) | 2005-02-07 | 2006-08-07 | The University Of British Columbia | Apparatus and methods for concentrating and separating particles such as molecules |
| DE602005003768T2 (de) * | 2005-07-07 | 2008-04-10 | Agilent Technologies, Inc. - a Delaware Corporation -, Santa Clara | Modulares System für Gelelektrophoresegerät |
| EP1946094B1 (de) | 2005-11-02 | 2011-04-20 | Agilent Technologies, Inc. | Kraftunterstützte probenwiedergewinnung in der gelelektrophorese |
| GB0607205D0 (en) * | 2006-04-10 | 2006-05-17 | Diagnoswiss Sa | Miniaturised biosensor with optimized anperimetric detection |
| JP2009539101A (ja) * | 2006-06-02 | 2009-11-12 | コーニンクレッカ フィリップス エレクトロニクス エヌ ヴィ | 等電点電気泳動による分離装置 |
| CN101868425B (zh) * | 2007-08-27 | 2013-01-02 | 技术研究及发展基金有限公司 | 通过电解控制pH梯度以及它们在等电聚焦中的用途 |
| US8475641B2 (en) | 2008-02-01 | 2013-07-02 | The University Of British Columbia | Methods and apparatus for particle introduction and recovery |
| EP2146200A1 (de) | 2008-07-16 | 2010-01-20 | Agilent Technologies, Inc. | Vorrichtung und Verfahren zur isoelektrischen Fokussierung |
| WO2010121381A1 (en) | 2009-04-21 | 2010-10-28 | The University Of British Columbia | System and methods for detection of particles |
| CN101556261B (zh) * | 2009-05-25 | 2012-01-11 | 北京理工大学 | 微管内等电聚焦和剪断吹出培养测定微生物等电点的方法 |
| JP5635609B2 (ja) | 2009-08-18 | 2014-12-03 | テクニオン リサーチ アンド ディベロップメント ファウンデーションリミテッド | 分子状分析物を分離する方法およびデバイス |
| AU2010286047B2 (en) * | 2009-08-18 | 2014-09-25 | Technion Research & Development Foundation Ltd. | Proton concentration topographies, methods and devices for producing the same |
| US9855735B2 (en) | 2009-11-23 | 2018-01-02 | Cyvek, Inc. | Portable microfluidic assay devices and methods of manufacture and use |
| US9700889B2 (en) * | 2009-11-23 | 2017-07-11 | Cyvek, Inc. | Methods and systems for manufacture of microarray assay systems, conducting microfluidic assays, and monitoring and scanning to obtain microfluidic assay results |
| US10065403B2 (en) | 2009-11-23 | 2018-09-04 | Cyvek, Inc. | Microfluidic assay assemblies and methods of manufacture |
| WO2013134742A2 (en) | 2012-03-08 | 2013-09-12 | Cyvek, Inc | Micro-tube particles for microfluidic assays and methods of manufacture |
| US9651568B2 (en) | 2009-11-23 | 2017-05-16 | Cyvek, Inc. | Methods and systems for epi-fluorescent monitoring and scanning for microfluidic assays |
| US9759718B2 (en) | 2009-11-23 | 2017-09-12 | Cyvek, Inc. | PDMS membrane-confined nucleic acid and antibody/antigen-functionalized microlength tube capture elements, and systems employing them, and methods of their use |
| US9500645B2 (en) | 2009-11-23 | 2016-11-22 | Cyvek, Inc. | Micro-tube particles for microfluidic assays and methods of manufacture |
| CN103649759B (zh) | 2011-03-22 | 2016-08-31 | 西维克公司 | 微流体装置以及制造方法和用途 |
| CA2742327A1 (en) | 2011-05-20 | 2012-11-20 | The University Of British Columiba | Systems and methods for enhanced scoda |
| EP2773221B1 (de) | 2011-11-04 | 2019-01-09 | Bio-Rad Laboratories, Inc. | Gleichzeitige reinigung von zellkomponenten |
| CN104024856B (zh) * | 2011-11-04 | 2018-10-26 | 生物辐射实验室股份有限公司 | 基于pI的蛋白质分级 |
| US9766207B2 (en) | 2011-11-04 | 2017-09-19 | Bio-Rad Laboratories, Inc. | Affinity methods and compositions employing electronic control of pH |
| WO2013104994A2 (en) | 2012-01-13 | 2013-07-18 | The University Of British Columbia | Multiple arm apparatus and methods for separation of particles |
| CN104254390B (zh) | 2012-02-15 | 2017-05-10 | 生物辐射实验室股份有限公司 | pH和离子强度的电子控制 |
| US9321012B2 (en) | 2012-04-04 | 2016-04-26 | Bio-Rad Laboratories, Inc. | Electronic protein fractionation |
| US9512477B2 (en) | 2012-05-04 | 2016-12-06 | Boreal Genomics Inc. | Biomarker anaylsis using scodaphoresis |
| US9340835B2 (en) | 2013-03-15 | 2016-05-17 | Boreal Genomics Corp. | Method for separating homoduplexed and heteroduplexed nucleic acids |
| US11130986B2 (en) | 2015-05-20 | 2021-09-28 | Quantum-Si Incorporated | Method for isolating target nucleic acid using heteroduplex binding proteins |
| CN106582289B (zh) * | 2015-10-16 | 2021-02-05 | 中美华世通生物医药科技(武汉)有限公司 | 过滤系统和过滤方法 |
| US10228367B2 (en) | 2015-12-01 | 2019-03-12 | ProteinSimple | Segmented multi-use automated assay cartridge |
| CN107245093A (zh) * | 2017-08-09 | 2017-10-13 | 赖兵 | 一种蛋白质等电纯化装置及方法 |
| MX2022005183A (es) | 2019-10-29 | 2022-08-08 | Quantum Si Inc | Bombeo peristáltico de fluidos y métodos, sistemas y dispositivos asociados. |
Family Cites Families (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2815320A (en) | 1953-10-23 | 1957-12-03 | Kollsman Paul | Method of and apparatus for treating ionic fluids by dialysis |
| DE2051715B2 (de) | 1970-10-21 | 1976-01-15 | Trennkammer fuer die kontinuierliche ablenkungselektrophorese | |
| US3719580A (en) | 1971-06-04 | 1973-03-06 | R Roberts | Electrophoretic apparatus |
| GB1422118A (en) | 1972-01-04 | 1976-01-21 | Bovril Ltd | Vertical gel electrophoresis apparatus |
| US3888758A (en) | 1973-06-28 | 1975-06-10 | Sheik Arshad Saeed | Apparatus for large scale gel electrophoresis |
| US4148703A (en) | 1976-02-11 | 1979-04-10 | Morton Weintraub | Method of electrophoretic purification of enzymes and peptides by means of an adjustable, specialized, geometrically located electrode system |
| US4362612A (en) | 1978-04-18 | 1982-12-07 | University Patents, Inc. | Isoelectric focusing apparatus |
| CA1238296A (en) | 1984-08-02 | 1988-06-21 | Claude Hamelin | Electrophoresis system for multiple agarose slab gels |
| EP0287513B1 (de) | 1987-04-11 | 1992-12-02 | Ciba-Geigy Ag | Isoelektrisches Fokussierverfahren sowie Einrichtung zur Durchführung dieses Verfahrens |
| US5114555A (en) | 1988-01-05 | 1992-05-19 | Monsanto Company | Continuous isoelectric separation |
| US5126022A (en) * | 1990-02-28 | 1992-06-30 | Soane Tecnologies, Inc. | Method and device for moving molecules by the application of a plurality of electrical fields |
| US5104512A (en) | 1990-05-14 | 1992-04-14 | Labintelligence, Inc. | Gel electrophoresis system |
| US5149418A (en) | 1991-09-04 | 1992-09-22 | Apogee Designs, Ltd. | Vertical gel electrophoresis apparatus having universal gel assembly support structure |
| FI119756B (fi) | 1995-01-18 | 2009-03-13 | Alteon Inc | Tiatsoliumyhdisteiden käyttö pitkälle edenneen glykosylaation lopputuotteiden muodostumisen estossa ja suunnan muutoksessa |
| IT1272932B (it) * | 1995-01-24 | 1997-07-01 | Pier Giorgio Righetti | Reattore ad enzima immobilizzato |
| US5540826A (en) | 1995-03-15 | 1996-07-30 | Protein Technologies, Inc. | Multi-channel separation device |
| US5856100A (en) | 1995-12-08 | 1999-01-05 | The Institute Of Physical And Chemical Research | Method for purification and transfer to separation/detection systems of DNA sequencing samples and plates used therefor |
| US5773645A (en) | 1997-05-05 | 1998-06-30 | Bio-Rad Laboratories, Inc. | Two-dimensional electrophoresis device |
| US6013165A (en) | 1998-05-22 | 2000-01-11 | Lynx Therapeutics, Inc. | Electrophoresis apparatus and method |
| AUPP576598A0 (en) * | 1998-09-07 | 1998-10-01 | Life Therapeutics Limited | Cassette for macromolecule purification |
| WO2000074850A2 (en) | 1999-06-03 | 2000-12-14 | University Of Washington | Microfluidic devices for transverse electrophoresis and isoelectric focusing |
| WO2001068225A1 (en) | 2000-03-15 | 2001-09-20 | Proteosys Ag | Micropreparative isoelectric focussing |
| US6638408B1 (en) | 2000-04-03 | 2003-10-28 | The Wistar Institute | Method and device for separation of charged molecules by solution isoelectric focusing |
| GB0010957D0 (en) | 2000-05-05 | 2000-06-28 | Novartis Ag | Compound & method |
| US6706162B1 (en) | 2000-09-25 | 2004-03-16 | Applera Corporation | High speed, high resolution compositions, methods, and kits for capillary electrophoresis |
-
2001
- 2001-08-31 GB GBGB0121189.5A patent/GB0121189D0/en not_active Ceased
-
2002
- 2002-08-30 DE DE60206937T patent/DE60206937T2/de not_active Expired - Lifetime
- 2002-08-30 EP EP02772306A patent/EP1421373B1/de not_active Expired - Lifetime
- 2002-08-30 WO PCT/EP2002/010369 patent/WO2003019172A2/en not_active Ceased
- 2002-08-30 US US10/486,181 patent/US7390389B2/en not_active Expired - Fee Related
- 2002-08-30 AT AT02772306T patent/ATE308042T1/de not_active IP Right Cessation
- 2002-08-30 JP JP2003523988A patent/JP4227016B2/ja not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| DE60206937T2 (de) | 2006-07-27 |
| US20040231986A1 (en) | 2004-11-25 |
| GB0121189D0 (en) | 2001-10-24 |
| EP1421373A2 (de) | 2004-05-26 |
| JP2005502864A (ja) | 2005-01-27 |
| WO2003019172A3 (en) | 2003-12-18 |
| DE60206937D1 (de) | 2005-12-01 |
| EP1421373B1 (de) | 2005-10-26 |
| WO2003019172A2 (en) | 2003-03-06 |
| US7390389B2 (en) | 2008-06-24 |
| JP4227016B2 (ja) | 2009-02-18 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |