ATE340631T1 - Verfahren zur isoelektrischen auftrennung mittels ph-wert-voreinstellung - Google Patents

Verfahren zur isoelektrischen auftrennung mittels ph-wert-voreinstellung

Info

Publication number
ATE340631T1
ATE340631T1 AT03757314T AT03757314T ATE340631T1 AT E340631 T1 ATE340631 T1 AT E340631T1 AT 03757314 T AT03757314 T AT 03757314T AT 03757314 T AT03757314 T AT 03757314T AT E340631 T1 ATE340631 T1 AT E340631T1
Authority
AT
Austria
Prior art keywords
value
isoelectric
catholyte
anolyte
ampholytic
Prior art date
Application number
AT03757314T
Other languages
English (en)
Inventor
Gyula Vigh
Original Assignee
Texas A & M Univ Sys
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Texas A & M Univ Sys filed Critical Texas A & M Univ Sys
Application granted granted Critical
Publication of ATE340631T1 publication Critical patent/ATE340631T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44795—Isoelectric focusing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D57/00—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C
    • B01D57/02—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C by electrophoresis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/24—Extraction; Separation; Purification by electrochemical means
    • C07K1/26—Electrophoresis

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Electrochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Environmental & Geological Engineering (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Electrostatic Separation (AREA)
  • Sampling And Sample Adjustment (AREA)
AT03757314T 2002-06-05 2003-06-03 Verfahren zur isoelektrischen auftrennung mittels ph-wert-voreinstellung ATE340631T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US10/163,885 US20030226752A1 (en) 2002-06-05 2002-06-05 Method for pH-biased isoelectric trapping separation

Publications (1)

Publication Number Publication Date
ATE340631T1 true ATE340631T1 (de) 2006-10-15

Family

ID=29710072

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03757314T ATE340631T1 (de) 2002-06-05 2003-06-03 Verfahren zur isoelektrischen auftrennung mittels ph-wert-voreinstellung

Country Status (9)

Country Link
US (2) US20030226752A1 (de)
EP (1) EP1558368B1 (de)
JP (1) JP2005528211A (de)
CN (1) CN1668367A (de)
AT (1) ATE340631T1 (de)
AU (1) AU2003256261B2 (de)
CA (1) CA2488380A1 (de)
DE (1) DE60308716T2 (de)
WO (1) WO2003103811A1 (de)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5221529B2 (ja) * 2006-06-20 2013-06-26 ベクトン・ディキンソン・アンド・カンパニー 電気泳動を利用してある種のタンパク質および粒子を分離して枯渇させる方法および装置
US20080272002A1 (en) * 2007-05-03 2008-11-06 Protein Forest, Inc. System and Method for Proteomics
WO2009147554A1 (en) * 2008-05-27 2009-12-10 Koninklijke Philips Electronics N. V. Isoelectric focusing biochip
CN102824854A (zh) * 2011-06-15 2012-12-19 杜权 一种电泳装置及其应用
WO2013028924A1 (en) 2011-08-23 2013-02-28 Board Of Regents, The University Of Texas System Electrolytic buffer generator
US9689841B2 (en) 2011-08-26 2017-06-27 The Texas A&M University System Fluorescent pI markers for isoelectric focusing separations and fluorescent labeling
WO2013067509A1 (en) 2011-11-04 2013-05-10 Bio-Rad Laboratories, Inc. Simultaneous purification of cell components
US9766207B2 (en) 2011-11-04 2017-09-19 Bio-Rad Laboratories, Inc. Affinity methods and compositions employing electronic control of pH
CZ303688B6 (cs) * 2012-02-14 2013-03-13 Ústav analytické chemie AV CR, v.v.i. Zpusob separace a zkoncentrování amfolytu isoelektrickou fokusací a zarízení pro provádení tohoto zpusobu
WO2013123425A1 (en) 2012-02-15 2013-08-22 Bio-Rad Laboratories, Inc. Electronic control of ph and ionic strength
US9321012B2 (en) 2012-04-04 2016-04-26 Bio-Rad Laboratories, Inc. Electronic protein fractionation
CN104056468B (zh) * 2014-06-25 2016-01-13 上海交通大学 无需两性电解质的分离两性物质的电聚焦方法
JP6153972B2 (ja) 2014-07-09 2017-06-28 アークレイ株式会社 緩衝剤組成物
US10962537B2 (en) 2016-04-22 2021-03-30 Memphasys Limited Biocompatible polymeric membranes
CN107899422B (zh) * 2017-11-09 2021-01-29 常州大学 一种共混离子交换膜的制备和应用方法
CN115536729A (zh) * 2022-08-24 2022-12-30 中新国际联合研究院 一种具有降尿酸活性的二肽及其编码基因与应用

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE415731B (sv) * 1977-04-26 1980-10-27 Pharmacia Fine Chemicals Ab Vattenloslig amfolyt for separationsendamal samt sett att framstella densamma
WO1979000002A1 (en) * 1977-06-15 1979-01-11 Nat Res Dev Improvements relating to membrane electrophoresis
US4319975A (en) * 1980-10-20 1982-03-16 Fmc Corporation Derivatized agarose and method of making and using same
US4707233A (en) * 1985-04-29 1987-11-17 Gradiant Pty Limited Device for extraction of electrophoretic fractions
US4804686A (en) * 1986-05-13 1989-02-14 Purdue Research Foundation Cation-exchange support materials and method
GB2225339A (en) * 1988-11-15 1990-05-30 Aligena Ag Separating electrically charged macromolecular compounds by forced-flow membrane electrophoresis
US5110434A (en) * 1990-12-20 1992-05-05 Bio-Rad Laboratories, Inc. Use of zwitterions to mobilize isoelectrically focused ampholyte zones
US5447612A (en) * 1993-02-01 1995-09-05 Protein Technologies, Inc. Buffering system and its use in electrophoretic processes
US6638408B1 (en) * 2000-04-03 2003-10-28 The Wistar Institute Method and device for separation of charged molecules by solution isoelectric focusing
US6923896B2 (en) * 2000-09-22 2005-08-02 The Texas A&M University System Electrophoresis apparatus and method
AUPR161400A0 (en) * 2000-11-22 2000-12-14 Life Therapeutics Limited Apparatus and method for isoelectric focusing separations
SE0201655D0 (sv) * 2002-05-31 2002-05-31 Amersham Biosciences Ab A method of electrophoresis

Also Published As

Publication number Publication date
JP2005528211A (ja) 2005-09-22
US20030226752A1 (en) 2003-12-11
AU2003256261B2 (en) 2006-02-16
DE60308716D1 (de) 2006-11-09
US20060151325A1 (en) 2006-07-13
WO2003103811A1 (en) 2003-12-18
CN1668367A (zh) 2005-09-14
AU2003256261A1 (en) 2003-12-22
EP1558368A1 (de) 2005-08-03
EP1558368B1 (de) 2006-09-27
DE60308716T2 (de) 2007-08-16
CA2488380A1 (en) 2003-12-18

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