ATE309336T1 - Umkehrbare modifikation von thermostabilen enzymen - Google Patents

Umkehrbare modifikation von thermostabilen enzymen

Info

Publication number
ATE309336T1
ATE309336T1 AT99110426T AT99110426T ATE309336T1 AT E309336 T1 ATE309336 T1 AT E309336T1 AT 99110426 T AT99110426 T AT 99110426T AT 99110426 T AT99110426 T AT 99110426T AT E309336 T1 ATE309336 T1 AT E309336T1
Authority
AT
Austria
Prior art keywords
nucleic acid
thermostable enzymes
enzyme
reversible modification
amplification
Prior art date
Application number
AT99110426T
Other languages
English (en)
Inventor
Igor Ivanov
Dirk Loeffert
Jie Kang
Joachim Ribbe
Kerstin Steinert
Original Assignee
Qiagen Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qiagen Gmbh filed Critical Qiagen Gmbh
Application granted granted Critical
Publication of ATE309336T1 publication Critical patent/ATE309336T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/99—Enzyme inactivation by chemical treatment
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT99110426T 1998-05-29 1999-05-28 Umkehrbare modifikation von thermostabilen enzymen ATE309336T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US8684698A 1998-05-29 1998-05-29
US09/183,950 US6183998B1 (en) 1998-05-29 1998-10-31 Method for reversible modification of thermostable enzymes

Publications (1)

Publication Number Publication Date
ATE309336T1 true ATE309336T1 (de) 2005-11-15

Family

ID=26775213

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99110426T ATE309336T1 (de) 1998-05-29 1999-05-28 Umkehrbare modifikation von thermostabilen enzymen

Country Status (6)

Country Link
US (1) US6183998B1 (de)
EP (1) EP0962526B1 (de)
JP (1) JP4398004B2 (de)
AT (1) ATE309336T1 (de)
DE (1) DE69928169T2 (de)
ES (1) ES2253848T3 (de)

Families Citing this family (81)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6828094B2 (en) * 1996-12-20 2004-12-07 Roche Diagnostics Gmbh Method for the uncoupled, direct, exponential amplification and sequencing of DNA molecules with the addition of a second thermostable DNA polymerase and its application
EP1175501A4 (de) * 1999-05-12 2002-11-27 Invitrogen Corp Zusammensetzungen und verfahren zur erhöhung der empfindlichkeit und spezifität der nukleinsäuresynthese
GB9920194D0 (en) * 1999-08-27 1999-10-27 Advanced Biotech Ltd A heat-stable thermostable DNA polymerase for use in nucleic acid amplification
US9771565B2 (en) 2000-05-26 2017-09-26 Life Technologies Corporation Thermostable reverse transcriptases and uses thereof
US7078208B2 (en) * 2000-05-26 2006-07-18 Invitrogen Corporation Thermostable reverse transcriptases and uses thereof
JP2001352982A (ja) * 2000-06-12 2001-12-25 Shimadzu Corp 核酸合成法
GB0110501D0 (en) 2001-04-30 2001-06-20 Secr Defence Brit Amplification process
US6667165B2 (en) 2001-11-13 2003-12-23 Eppendorf Ag Method and compositions for reversible inhibition of thermostable polymerases
US7772383B2 (en) * 2002-01-25 2010-08-10 The Trustees Of Princeton University Chemical PCR: Compositions for enhancing polynucleotide amplification reactions
US7148049B2 (en) 2002-04-02 2006-12-12 Roche Molecular Systems, Inc. Thermostable or thermoactive DNA polymerase molecules with attenuated 3′-5′ exonuclease activity
WO2004024749A2 (en) 2002-09-13 2004-03-25 Invitrogen Corporation Thermostable reverse transcriptases and uses thereof
EP1411133B1 (de) * 2002-09-24 2009-01-07 Qiagen GmbH Verbesserte Koamplifikation von Nukleinsäuren
EP1403379A1 (de) * 2002-09-24 2004-03-31 QIAGEN GmbH Verbesserte Koamplifikation von Nukleinsäuren
AU2004225520A1 (en) 2003-03-25 2004-10-14 Stratagene DNA polymerase fusions and uses thereof
DE10315640A1 (de) * 2003-04-04 2004-10-14 Ignatov, Konstantin Verfahren zur kontrollierten Freisetzung von Komponenten in eine Lösung
WO2004094666A1 (en) * 2003-04-24 2004-11-04 Dzieglewska, Hanna Allele-specific mutation detection assay
AU2004203649B2 (en) 2003-08-12 2006-01-12 F. Hoffmann-La Roche Ag Thermostable Taq polymerase fragment
US20060166363A1 (en) * 2004-01-27 2006-07-27 Sergei Zolotukhin Modified baculovirus expression system for production of pseudotyped rAAV vector
CA2568998A1 (en) 2004-06-04 2005-12-15 Takara Bio Inc. Polypeptides having dna polymerase activity
WO2005123913A2 (en) * 2004-06-09 2005-12-29 Nustar Laboratory Reversibly modified thermostable enzyme compositions and methods of making and using the same
US7700281B2 (en) * 2004-06-30 2010-04-20 Usb Corporation Hot start nucleic acid amplification
US20060228726A1 (en) * 2005-01-06 2006-10-12 Martin Patrick K Polypeptides having nucleic acid binding activity and compositions and methods for nucleic acid amplification
WO2006076650A2 (en) * 2005-01-12 2006-07-20 Applera Corporation Compositions, methods, and kits for selective amplification of nucleic acids
WO2006130632A2 (en) * 2005-05-31 2006-12-07 Invitrogen Corporation Separation and purification of nucleic acid from paraffin-containing samples
AU2006287548A1 (en) * 2005-09-06 2007-03-15 Gen-Probe Incorporated Methods, compositions and kits for isothermal amplification of nucleic acids
US20070059713A1 (en) * 2005-09-09 2007-03-15 Lee Jun E SSB-DNA polymerase fusion proteins
DE102005047617A1 (de) 2005-10-05 2007-04-19 Qiagen Gmbh Polymerase-Kettenreaktions-Verfahren unter Einsatz einer DNA-Polymerase mit Proofreading-Eigenschaft
GB0600228D0 (en) 2006-01-06 2006-02-15 Fermentas Uab Inactivation method
DE102006015960A1 (de) * 2006-04-04 2007-10-11 Qiagen Gmbh Gemisch reversibel inhibierter Enzyme
US8435774B2 (en) 2006-06-28 2013-05-07 Qiagen Gmbh Enhancing reactivation of thermostable reversibly inactivated enzymes
CN106566855B (zh) * 2006-07-31 2021-11-09 苏州新海生物科技股份有限公司 使用可逆修饰寡核苷酸扩增核酸
US9045522B2 (en) 2006-07-31 2015-06-02 Wanli Bi Nucleic acid amplification using a reversibly modified oligonucleotide
US20100129878A1 (en) * 2007-04-25 2010-05-27 Parthasarathy Ranjani V Methods for nucleic acid amplification
WO2009029648A2 (en) * 2007-08-27 2009-03-05 Applera Corporation Methods and compositions for pcr
DE102008047790A1 (de) 2008-09-17 2010-04-15 Qiagen Gmbh Verfahren zur Normierung des Gehalts von Biomolekülen in einer Probe
US9834815B2 (en) 2009-03-25 2017-12-05 Life Technologies Corporation Discriminatory positive/extraction control DNA
WO2011044444A2 (en) * 2009-10-09 2011-04-14 Life Technologies Corporation cDNA SYNTHESIS USING A REVERSIBLY INACTIVATED REVERSE TRANSCRIPTASE
US9321999B2 (en) 2009-11-19 2016-04-26 Solis Biodyne Oü Compositions for increasing polypeptide stability and activity, and related methods
US8618253B2 (en) 2010-05-25 2013-12-31 Samsung Techwin Co., Ltd. Modified RNAse H and detection of nucleic acid amplification
US10323236B2 (en) 2011-07-22 2019-06-18 President And Fellows Of Harvard College Evaluation and improvement of nuclease cleavage specificity
CA2905429A1 (en) 2013-03-14 2014-10-02 Abbott Molecular Inc. Minimizing errors using uracil-dna-n-glycosylase
US9163284B2 (en) 2013-08-09 2015-10-20 President And Fellows Of Harvard College Methods for identifying a target site of a Cas9 nuclease
US9228207B2 (en) 2013-09-06 2016-01-05 President And Fellows Of Harvard College Switchable gRNAs comprising aptamers
US9322037B2 (en) 2013-09-06 2016-04-26 President And Fellows Of Harvard College Cas9-FokI fusion proteins and uses thereof
KR101488110B1 (ko) 2013-11-29 2015-01-29 성균관대학교산학협력단 나노아케움 이퀴탄스 유래의 Neq HS DNA 중합효소의 돌연변이체 제조 및 이를 이용한 hot-start PCR 응용
JP2017503521A (ja) 2014-01-22 2017-02-02 ライフ テクノロジーズ コーポレーション 高温核酸合成で使用するための新規な逆転写酵素
US10077453B2 (en) 2014-07-30 2018-09-18 President And Fellows Of Harvard College CAS9 proteins including ligand-dependent inteins
JP6822974B2 (ja) 2015-05-29 2021-01-27 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ シトラコン酸無水物によって微生物を不活性化する方法
IL310721B2 (en) 2015-10-23 2025-11-01 Harvard College Nucleobase editors and uses thereof
CN116144626A (zh) 2016-01-13 2023-05-23 新英格兰生物实验室公司 T7 rna聚合酶的热稳定变体
JP7106461B2 (ja) 2016-06-07 2022-07-26 セファイド 熱安定性ポリメラーゼ阻害剤組成物及び方法
CN110214183A (zh) 2016-08-03 2019-09-06 哈佛大学的校长及成员们 腺苷核碱基编辑器及其用途
WO2018031683A1 (en) 2016-08-09 2018-02-15 President And Fellows Of Harvard College Programmable cas9-recombinase fusion proteins and uses thereof
US11542509B2 (en) 2016-08-24 2023-01-03 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
KR102622411B1 (ko) 2016-10-14 2024-01-10 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵염기 에디터의 aav 전달
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
US12390514B2 (en) 2017-03-09 2025-08-19 President And Fellows Of Harvard College Cancer vaccine
EP3592853A1 (de) 2017-03-09 2020-01-15 President and Fellows of Harvard College Unterdrückung von schmerzen durch geneditierung
US11542496B2 (en) 2017-03-10 2023-01-03 President And Fellows Of Harvard College Cytosine to guanine base editor
KR20240116572A (ko) 2017-03-23 2024-07-29 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제
US11560566B2 (en) 2017-05-12 2023-01-24 President And Fellows Of Harvard College Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation
CN111801345A (zh) 2017-07-28 2020-10-20 哈佛大学的校长及成员们 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物
EP3676376B1 (de) 2017-08-30 2025-01-15 President and Fellows of Harvard College Hocheffiziente baseneditoren mit gam
KR20250107288A (ko) 2017-10-16 2025-07-11 더 브로드 인스티튜트, 인코퍼레이티드 아데노신 염기 편집제의 용도
US12406749B2 (en) 2017-12-15 2025-09-02 The Broad Institute, Inc. Systems and methods for predicting repair outcomes in genetic engineering
US12157760B2 (en) 2018-05-23 2024-12-03 The Broad Institute, Inc. Base editors and uses thereof
US12522807B2 (en) 2018-07-09 2026-01-13 The Broad Institute, Inc. RNA programmable epigenetic RNA modifiers and uses thereof
WO2020092453A1 (en) 2018-10-29 2020-05-07 The Broad Institute, Inc. Nucleobase editors comprising geocas9 and uses thereof
CN111229023B (zh) * 2018-11-29 2022-01-28 内蒙古东盛硅藻土科技创新产业园有限公司 一种化学降解添加剂
US12351837B2 (en) 2019-01-23 2025-07-08 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
WO2020191233A1 (en) 2019-03-19 2020-09-24 The Broad Institute, Inc. Methods and compositions for editing nucleotide sequences
US12473543B2 (en) 2019-04-17 2025-11-18 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
US12435330B2 (en) 2019-10-10 2025-10-07 The Broad Institute, Inc. Methods and compositions for prime editing RNA
IL297761A (en) 2020-05-08 2022-12-01 Broad Inst Inc Methods and compositions for simultaneously editing two helices of a designated double-helix nucleotide sequence
EP4204568A4 (de) 2020-08-28 2024-09-11 Duke University Manipulierte polymerasen und verfahren zur verwendung davon
CA3193099A1 (en) 2020-09-24 2022-03-31 David R. Liu Prime editing guide rnas, compositions thereof, and methods of using the same
EP4274894A2 (de) 2021-01-11 2023-11-15 The Broad Institute, Inc. Prime-editor-varianten, konstrukte und verfahren zur verbesserung der prime-editierungseffizienz und -präzision
WO2023076898A1 (en) 2021-10-25 2023-05-04 The Broad Institute, Inc. Methods and compositions for editing a genome with prime editing and a recombinase
US20250145989A1 (en) 2022-01-26 2025-05-08 Seegene, Inc. Method for detecting target nucleic acids in sample
CN116064468B (zh) * 2022-11-04 2023-11-21 南京诺唯赞生物科技股份有限公司 一种化学修饰Taq酶储存液
EP4619515A1 (de) 2022-11-17 2025-09-24 The Broad Institute, Inc. Prime-editor-bereitstellung durch aav

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
KR100204837B1 (ko) * 1989-04-28 1999-06-15 기스트 브로카데스 나암로제 베노오트하프 카르복실 에스테라제의 안정화
DK0515506T3 (da) 1990-02-16 2000-05-08 Hoffmann La Roche Fremgangsmåde til påvisning af carcinogene humane papillomavirus
US5338671A (en) 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
AU690124B2 (en) * 1993-06-09 1998-04-23 Gamera Bioscience Corporation Magnetic cycle reaction
DE69531487T2 (de) * 1994-01-31 2004-06-17 The Regents Of The University Of California, Oakland Methode zur eliminierung von sequenzierartefakten
US5773258A (en) * 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme

Also Published As

Publication number Publication date
EP0962526A3 (de) 2002-01-16
DE69928169T2 (de) 2006-08-03
US6183998B1 (en) 2001-02-06
JP4398004B2 (ja) 2010-01-13
EP0962526B1 (de) 2005-11-09
JP2000004878A (ja) 2000-01-11
DE69928169D1 (de) 2005-12-15
EP0962526A2 (de) 1999-12-08
ES2253848T3 (es) 2006-06-01

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