ATE299530T1 - Reversible inaktivierung von enzymen - Google Patents
Reversible inaktivierung von enzymenInfo
- Publication number
- ATE299530T1 ATE299530T1 AT00307337T AT00307337T ATE299530T1 AT E299530 T1 ATE299530 T1 AT E299530T1 AT 00307337 T AT00307337 T AT 00307337T AT 00307337 T AT00307337 T AT 00307337T AT E299530 T1 ATE299530 T1 AT E299530T1
- Authority
- AT
- Austria
- Prior art keywords
- ligase
- dna polymerase
- enzymes
- dicarboxylic acid
- acid anhydride
- Prior art date
Links
- 102000004190 Enzymes Human genes 0.000 title abstract 2
- 108090000790 Enzymes Proteins 0.000 title abstract 2
- 230000002779 inactivation Effects 0.000 title abstract 2
- 230000002441 reversible effect Effects 0.000 title 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 abstract 3
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 abstract 3
- 102000003960 Ligases Human genes 0.000 abstract 3
- 108090000364 Ligases Proteins 0.000 abstract 3
- OFOBLEOULBTSOW-UHFFFAOYSA-N Malonic acid Chemical compound OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 230000000694 effects Effects 0.000 abstract 1
- 230000000415 inactivating effect Effects 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 239000003960 organic solvent Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/99—Enzyme inactivation by chemical treatment
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6862—Ligase chain reaction [LCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Paints Or Removers (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB9920194.9A GB9920194D0 (en) | 1999-08-27 | 1999-08-27 | A heat-stable thermostable DNA polymerase for use in nucleic acid amplification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE299530T1 true ATE299530T1 (de) | 2005-07-15 |
Family
ID=10859830
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT00307337T ATE299530T1 (de) | 1999-08-27 | 2000-08-25 | Reversible inaktivierung von enzymen |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US6479264B1 (de) |
| EP (1) | EP1078984B1 (de) |
| AT (1) | ATE299530T1 (de) |
| DE (1) | DE60021234T2 (de) |
| GB (2) | GB9920194D0 (de) |
Families Citing this family (55)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1401850A1 (de) * | 2001-06-20 | 2004-03-31 | Nuevolution A/S | Nukleosidderivate zur herstellung einer verbindungsbibliothek |
| ES2193843B1 (es) * | 2001-07-06 | 2005-03-01 | Consejo Superior De Investigaciones Cientificas | Procedimiento para la inactivacion reversible por calor, e in vivo, de proteinas con actividad biologica. |
| CA2409775C (en) * | 2001-12-03 | 2010-07-13 | F. Hoffmann-La Roche Ag | Reversibly modified thermostable enzymes for dna synthesis and amplification in vitro |
| IL163822A0 (en) | 2002-03-15 | 2005-12-18 | Nuevolution As | An improved method for synthesising templated molecules |
| AU2003247266A1 (en) | 2002-08-01 | 2004-02-23 | Nuevolution A/S | Multi-step synthesis of templated molecules |
| AU2003266932B2 (en) * | 2002-09-27 | 2009-04-09 | Mpm-Holding Aps | Spatially encoded polymer matrix |
| PT1558744E (pt) | 2002-10-30 | 2011-09-22 | Nuevolution As | Codificação enzimática |
| WO2004056994A2 (en) | 2002-12-19 | 2004-07-08 | Nuevolution A/S | Quasirandom structure and function guided synthesis methods |
| EP1597395A2 (de) | 2003-02-21 | 2005-11-23 | Nuevolution A/S | Methode zur herstellung einer bibliothek der zweiten generation |
| DE602004025801D1 (de) | 2003-03-25 | 2010-04-15 | Stratagene California | Dna-polymerasefusionen und deren verwendungen |
| AU2004203649B2 (en) | 2003-08-12 | 2006-01-12 | F. Hoffmann-La Roche Ag | Thermostable Taq polymerase fragment |
| DE602004023960D1 (de) | 2003-09-18 | 2009-12-17 | Nuevolution As | Methode zur Gewinnung struktureller Informationen kodierter Moleküle und zur Selektion von Verbindungen |
| CN101426908B (zh) * | 2004-06-09 | 2014-06-11 | 纽星实验室 | 可逆性修饰的热稳定酶组合物及其制备方法和使用方法 |
| US7700281B2 (en) * | 2004-06-30 | 2010-04-20 | Usb Corporation | Hot start nucleic acid amplification |
| US9574189B2 (en) | 2005-12-01 | 2017-02-21 | Nuevolution A/S | Enzymatic encoding methods for efficient synthesis of large libraries |
| GB0600228D0 (en) | 2006-01-06 | 2006-02-15 | Fermentas Uab | Inactivation method |
| WO2007137291A1 (en) * | 2006-05-23 | 2007-11-29 | Molecular Detection, Inc. | Ambient temperature stable kits for molecular diagnostics |
| CN102149824A (zh) | 2008-07-03 | 2011-08-10 | 阿莱洛吉克生物科学公司 | 保护亲核基团的组合物和方法 |
| DE102009030491A1 (de) | 2009-03-18 | 2010-09-23 | W.E.T. Automotive Systems Ag | Klimatisierungseinrichtung für einen klimatisierten Gegenstand in einem Fahrzeuginnenraum |
| ES2713873T3 (es) | 2010-04-16 | 2019-05-24 | Nuevolution As | Complejos bifuncionales y métodos para hacer y utilizar tales complejos |
| EP2734621B1 (de) | 2011-07-22 | 2019-09-04 | President and Fellows of Harvard College | Beurteilung und verbesserung einer nukleasespaltungsspezifität |
| GB201217405D0 (en) * | 2012-09-28 | 2012-11-14 | Fermentas Uab | Protein removal agent |
| US9163284B2 (en) | 2013-08-09 | 2015-10-20 | President And Fellows Of Harvard College | Methods for identifying a target site of a Cas9 nuclease |
| US9340800B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | Extended DNA-sensing GRNAS |
| US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
| CA2956224A1 (en) | 2014-07-30 | 2016-02-11 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
| WO2016193070A1 (en) | 2015-05-29 | 2016-12-08 | Roche Diagnostics Gmbh | A method of inactivating microbes by citraconic anhydride |
| EP4434589A3 (de) | 2015-10-23 | 2025-05-14 | President and Fellows of Harvard College | Entwickelte cas9-proteine für geneditierung |
| JP7231935B2 (ja) | 2016-08-03 | 2023-03-08 | プレジデント アンド フェローズ オブ ハーバード カレッジ | アデノシン核酸塩基編集因子およびそれらの使用 |
| US11661590B2 (en) | 2016-08-09 | 2023-05-30 | President And Fellows Of Harvard College | Programmable CAS9-recombinase fusion proteins and uses thereof |
| WO2018039438A1 (en) | 2016-08-24 | 2018-03-01 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| AU2017342543B2 (en) | 2016-10-14 | 2024-06-27 | President And Fellows Of Harvard College | AAV delivery of nucleobase editors |
| WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
| CN110662556A (zh) | 2017-03-09 | 2020-01-07 | 哈佛大学的校长及成员们 | 癌症疫苗 |
| US11898179B2 (en) | 2017-03-09 | 2024-02-13 | President And Fellows Of Harvard College | Suppression of pain by gene editing |
| JP2020510439A (ja) | 2017-03-10 | 2020-04-09 | プレジデント アンド フェローズ オブ ハーバード カレッジ | シトシンからグアニンへの塩基編集因子 |
| WO2018176009A1 (en) | 2017-03-23 | 2018-09-27 | President And Fellows Of Harvard College | Nucleobase editors comprising nucleic acid programmable dna binding proteins |
| US11560566B2 (en) | 2017-05-12 | 2023-01-24 | President And Fellows Of Harvard College | Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation |
| US11732274B2 (en) | 2017-07-28 | 2023-08-22 | President And Fellows Of Harvard College | Methods and compositions for evolving base editors using phage-assisted continuous evolution (PACE) |
| WO2019139645A2 (en) | 2017-08-30 | 2019-07-18 | President And Fellows Of Harvard College | High efficiency base editors comprising gam |
| KR20200121782A (ko) | 2017-10-16 | 2020-10-26 | 더 브로드 인스티튜트, 인코퍼레이티드 | 아데노신 염기 편집제의 용도 |
| US12406749B2 (en) | 2017-12-15 | 2025-09-02 | The Broad Institute, Inc. | Systems and methods for predicting repair outcomes in genetic engineering |
| US12157760B2 (en) | 2018-05-23 | 2024-12-03 | The Broad Institute, Inc. | Base editors and uses thereof |
| US12522807B2 (en) | 2018-07-09 | 2026-01-13 | The Broad Institute, Inc. | RNA programmable epigenetic RNA modifiers and uses thereof |
| WO2020092453A1 (en) | 2018-10-29 | 2020-05-07 | The Broad Institute, Inc. | Nucleobase editors comprising geocas9 and uses thereof |
| WO2020154500A1 (en) | 2019-01-23 | 2020-07-30 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| MX2021011426A (es) | 2019-03-19 | 2022-03-11 | Broad Inst Inc | Metodos y composiciones para editar secuencias de nucleótidos. |
| WO2020214842A1 (en) | 2019-04-17 | 2020-10-22 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| US12435330B2 (en) | 2019-10-10 | 2025-10-07 | The Broad Institute, Inc. | Methods and compositions for prime editing RNA |
| WO2021226558A1 (en) | 2020-05-08 | 2021-11-11 | The Broad Institute, Inc. | Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence |
| JP2023543803A (ja) | 2020-09-24 | 2023-10-18 | ザ ブロード インスティテュート，インコーポレーテッド | プライム編集ガイドｒｎａ、その組成物、及びその使用方法 |
| WO2022150790A2 (en) | 2021-01-11 | 2022-07-14 | The Broad Institute, Inc. | Prime editor variants, constructs, and methods for enhancing prime editing efficiency and precision |
| WO2023076898A1 (en) | 2021-10-25 | 2023-05-04 | The Broad Institute, Inc. | Methods and compositions for editing a genome with prime editing and a recombinase |
| CN116410951A (zh) * | 2021-12-30 | 2023-07-11 | 广州达安基因股份有限公司 | 一种提高化学修饰的Taq酶的活性的方法和经该方法处理的Taq酶 |
| EP4619515A1 (de) | 2022-11-17 | 2025-09-24 | The Broad Institute, Inc. | Prime-editor-bereitstellung durch aav |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2674248B1 (fr) * | 1991-03-19 | 1993-06-11 | Elf Aquitaine | Procede de modification chimique des proteines. |
| US5773258A (en) * | 1995-08-25 | 1998-06-30 | Roche Molecular Systems, Inc. | Nucleic acid amplification using a reversibly inactivated thermostable enzyme |
| JPH10276776A (ja) * | 1997-04-07 | 1998-10-20 | Toyobo Co Ltd | 可逆的に不活化された耐熱性ｄｎａポリメラーゼ |
| US6183998B1 (en) * | 1998-05-29 | 2001-02-06 | Qiagen Gmbh Max-Volmer-Strasse 4 | Method for reversible modification of thermostable enzymes |
-
1999
- 1999-08-27 GB GBGB9920194.9A patent/GB9920194D0/en not_active Ceased
-
2000
- 2000-08-25 GB GB0021086A patent/GB2353530B/en not_active Expired - Fee Related
- 2000-08-25 US US09/649,707 patent/US6479264B1/en not_active Expired - Lifetime
- 2000-08-25 AT AT00307337T patent/ATE299530T1/de not_active IP Right Cessation
- 2000-08-25 DE DE60021234T patent/DE60021234T2/de not_active Expired - Lifetime
- 2000-08-25 EP EP00307337A patent/EP1078984B1/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| GB2353530A (en) | 2001-02-28 |
| EP1078984B1 (de) | 2005-07-13 |
| GB9920194D0 (en) | 1999-10-27 |
| DE60021234T2 (de) | 2006-04-27 |
| GB0021086D0 (en) | 2000-10-11 |
| EP1078984A1 (de) | 2001-02-28 |
| DE60021234D1 (de) | 2005-08-18 |
| GB2353530B (en) | 2001-06-27 |
| US6479264B1 (en) | 2002-11-12 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |