ATE299530T1 - Reversible inaktivierung von enzymen - Google Patents
Reversible inaktivierung von enzymenInfo
- Publication number
- ATE299530T1 ATE299530T1 AT00307337T AT00307337T ATE299530T1 AT E299530 T1 ATE299530 T1 AT E299530T1 AT 00307337 T AT00307337 T AT 00307337T AT 00307337 T AT00307337 T AT 00307337T AT E299530 T1 ATE299530 T1 AT E299530T1
- Authority
- AT
- Austria
- Prior art keywords
- ligase
- dna polymerase
- enzymes
- dicarboxylic acid
- acid anhydride
- Prior art date
Links
- 102000004190 Enzymes Human genes 0.000 title abstract 2
- 108090000790 Enzymes Proteins 0.000 title abstract 2
- 230000002779 inactivation Effects 0.000 title abstract 2
- 230000002441 reversible effect Effects 0.000 title 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 abstract 3
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 abstract 3
- 102000003960 Ligases Human genes 0.000 abstract 3
- 108090000364 Ligases Proteins 0.000 abstract 3
- OFOBLEOULBTSOW-UHFFFAOYSA-N Malonic acid Chemical compound OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 230000000694 effects Effects 0.000 abstract 1
- 230000000415 inactivating effect Effects 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 239000003960 organic solvent Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/99—Enzyme inactivation by chemical treatment
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6862—Ligase chain reaction [LCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Paints Or Removers (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB9920194.9A GB9920194D0 (en) | 1999-08-27 | 1999-08-27 | A heat-stable thermostable DNA polymerase for use in nucleic acid amplification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE299530T1 true ATE299530T1 (de) | 2005-07-15 |
Family
ID=10859830
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT00307337T ATE299530T1 (de) | 1999-08-27 | 2000-08-25 | Reversible inaktivierung von enzymen |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US6479264B1 (de) |
| EP (1) | EP1078984B1 (de) |
| AT (1) | ATE299530T1 (de) |
| DE (1) | DE60021234T2 (de) |
| GB (2) | GB9920194D0 (de) |
Families Citing this family (56)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1401850A1 (de) | 2001-06-20 | 2004-03-31 | Nuevolution A/S | Nukleosidderivate zur herstellung einer verbindungsbibliothek |
| ES2193843B1 (es) * | 2001-07-06 | 2005-03-01 | Consejo Superior De Investigaciones Cientificas | Procedimiento para la inactivacion reversible por calor, e in vivo, de proteinas con actividad biologica. |
| CA2409775C (en) * | 2001-12-03 | 2010-07-13 | F. Hoffmann-La Roche Ag | Reversibly modified thermostable enzymes for dna synthesis and amplification in vitro |
| AU2003214031A1 (en) | 2002-03-15 | 2003-09-29 | Nuevolution A/S | An improved method for synthesising templated molecules |
| US10731151B2 (en) | 2002-03-15 | 2020-08-04 | Nuevolution A/S | Method for synthesising templated molecules |
| EP1539980B1 (de) | 2002-08-01 | 2016-02-17 | Nuevolution A/S | MEHRSTUFENSYNTHESE VON MIT MATRIZEN ASSOZIIERTE MOLEKüLEN |
| US8791053B2 (en) * | 2002-09-27 | 2014-07-29 | Mpm-Holding Aps | Spatially encoded polymer matrix |
| EP2348124B1 (de) | 2002-10-30 | 2013-12-11 | Nuevolution A/S | Synthese bifunktioneller Komplexe |
| WO2004056994A2 (en) | 2002-12-19 | 2004-07-08 | Nuevolution A/S | Quasirandom structure and function guided synthesis methods |
| US20070026397A1 (en) | 2003-02-21 | 2007-02-01 | Nuevolution A/S | Method for producing second-generation library |
| AU2004225520A1 (en) | 2003-03-25 | 2004-10-14 | Stratagene | DNA polymerase fusions and uses thereof |
| AU2004203649B2 (en) | 2003-08-12 | 2006-01-12 | F. Hoffmann-La Roche Ag | Thermostable Taq polymerase fragment |
| US11118215B2 (en) | 2003-09-18 | 2021-09-14 | Nuevolution A/S | Method for obtaining structural information concerning an encoded molecule and method for selecting compounds |
| WO2005123913A2 (en) * | 2004-06-09 | 2005-12-29 | Nustar Laboratory | Reversibly modified thermostable enzyme compositions and methods of making and using the same |
| US7700281B2 (en) * | 2004-06-30 | 2010-04-20 | Usb Corporation | Hot start nucleic acid amplification |
| PT3305900T (pt) | 2005-12-01 | 2021-10-27 | Nuevolution As | Métodos de codificação enzimática para síntese eficaz de bibliotecas grandes |
| GB0600228D0 (en) | 2006-01-06 | 2006-02-15 | Fermentas Uab | Inactivation method |
| EP2210955A3 (de) * | 2006-05-23 | 2010-09-15 | Molecular Detection, Inc. | Bei Umgebungstemperatur beständige Kits zur Molekulardiagnostik |
| CN102149824A (zh) | 2008-07-03 | 2011-08-10 | 阿莱洛吉克生物科学公司 | 保护亲核基团的组合物和方法 |
| DE102009030491A1 (de) | 2009-03-18 | 2010-09-23 | W.E.T. Automotive Systems Ag | Klimatisierungseinrichtung für einen klimatisierten Gegenstand in einem Fahrzeuginnenraum |
| EP3540059A1 (de) | 2010-04-16 | 2019-09-18 | Nuevolution A/S | Bifunktionelle komplexe und verfahren zur herstellung sowie verwendung derartiger komplexe |
| US10323236B2 (en) | 2011-07-22 | 2019-06-18 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| GB201217405D0 (en) | 2012-09-28 | 2012-11-14 | Fermentas Uab | Protein removal agent |
| US9163284B2 (en) | 2013-08-09 | 2015-10-20 | President And Fellows Of Harvard College | Methods for identifying a target site of a Cas9 nuclease |
| US9228207B2 (en) | 2013-09-06 | 2016-01-05 | President And Fellows Of Harvard College | Switchable gRNAs comprising aptamers |
| US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
| US10077453B2 (en) | 2014-07-30 | 2018-09-18 | President And Fellows Of Harvard College | CAS9 proteins including ligand-dependent inteins |
| JP6822974B2 (ja) | 2015-05-29 | 2021-01-27 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | シトラコン酸無水物によって微生物を不活性化する方法 |
| IL310721B2 (en) | 2015-10-23 | 2025-11-01 | Harvard College | Nucleobase editors and uses thereof |
| CN110214183A (zh) | 2016-08-03 | 2019-09-06 | 哈佛大学的校长及成员们 | 腺苷核碱基编辑器及其用途 |
| WO2018031683A1 (en) | 2016-08-09 | 2018-02-15 | President And Fellows Of Harvard College | Programmable cas9-recombinase fusion proteins and uses thereof |
| US11542509B2 (en) | 2016-08-24 | 2023-01-03 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| KR102622411B1 (ko) | 2016-10-14 | 2024-01-10 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵염기 에디터의 aav 전달 |
| WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
| US12390514B2 (en) | 2017-03-09 | 2025-08-19 | President And Fellows Of Harvard College | Cancer vaccine |
| EP3592853A1 (de) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Unterdrückung von schmerzen durch geneditierung |
| US11542496B2 (en) | 2017-03-10 | 2023-01-03 | President And Fellows Of Harvard College | Cytosine to guanine base editor |
| KR20240116572A (ko) | 2017-03-23 | 2024-07-29 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제 |
| US11560566B2 (en) | 2017-05-12 | 2023-01-24 | President And Fellows Of Harvard College | Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation |
| CN111801345A (zh) | 2017-07-28 | 2020-10-20 | 哈佛大学的校长及成员们 | 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物 |
| EP3676376B1 (de) | 2017-08-30 | 2025-01-15 | President and Fellows of Harvard College | Hocheffiziente baseneditoren mit gam |
| KR20250107288A (ko) | 2017-10-16 | 2025-07-11 | 더 브로드 인스티튜트, 인코퍼레이티드 | 아데노신 염기 편집제의 용도 |
| US12406749B2 (en) | 2017-12-15 | 2025-09-02 | The Broad Institute, Inc. | Systems and methods for predicting repair outcomes in genetic engineering |
| US12157760B2 (en) | 2018-05-23 | 2024-12-03 | The Broad Institute, Inc. | Base editors and uses thereof |
| US12522807B2 (en) | 2018-07-09 | 2026-01-13 | The Broad Institute, Inc. | RNA programmable epigenetic RNA modifiers and uses thereof |
| WO2020092453A1 (en) | 2018-10-29 | 2020-05-07 | The Broad Institute, Inc. | Nucleobase editors comprising geocas9 and uses thereof |
| US12351837B2 (en) | 2019-01-23 | 2025-07-08 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| WO2020191233A1 (en) | 2019-03-19 | 2020-09-24 | The Broad Institute, Inc. | Methods and compositions for editing nucleotide sequences |
| US12473543B2 (en) | 2019-04-17 | 2025-11-18 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| US12435330B2 (en) | 2019-10-10 | 2025-10-07 | The Broad Institute, Inc. | Methods and compositions for prime editing RNA |
| IL297761A (en) | 2020-05-08 | 2022-12-01 | Broad Inst Inc | Methods and compositions for simultaneously editing two helices of a designated double-helix nucleotide sequence |
| CA3193099A1 (en) | 2020-09-24 | 2022-03-31 | David R. Liu | Prime editing guide rnas, compositions thereof, and methods of using the same |
| EP4274894A2 (de) | 2021-01-11 | 2023-11-15 | The Broad Institute, Inc. | Prime-editor-varianten, konstrukte und verfahren zur verbesserung der prime-editierungseffizienz und -präzision |
| WO2023076898A1 (en) | 2021-10-25 | 2023-05-04 | The Broad Institute, Inc. | Methods and compositions for editing a genome with prime editing and a recombinase |
| CN116410951A (zh) * | 2021-12-30 | 2023-07-11 | 广州达安基因股份有限公司 | 一种提高化学修饰的Taq酶的活性的方法和经该方法处理的Taq酶 |
| EP4619515A1 (de) | 2022-11-17 | 2025-09-24 | The Broad Institute, Inc. | Prime-editor-bereitstellung durch aav |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2674248B1 (fr) * | 1991-03-19 | 1993-06-11 | Elf Aquitaine | Procede de modification chimique des proteines. |
| US5773258A (en) * | 1995-08-25 | 1998-06-30 | Roche Molecular Systems, Inc. | Nucleic acid amplification using a reversibly inactivated thermostable enzyme |
| JPH10276776A (ja) * | 1997-04-07 | 1998-10-20 | Toyobo Co Ltd | 可逆的に不活化された耐熱性ｄｎａポリメラーゼ |
| US6183998B1 (en) * | 1998-05-29 | 2001-02-06 | Qiagen Gmbh Max-Volmer-Strasse 4 | Method for reversible modification of thermostable enzymes |
-
1999
- 1999-08-27 GB GBGB9920194.9A patent/GB9920194D0/en not_active Ceased
-
2000
- 2000-08-25 US US09/649,707 patent/US6479264B1/en not_active Expired - Lifetime
- 2000-08-25 GB GB0021086A patent/GB2353530B/en not_active Expired - Fee Related
- 2000-08-25 AT AT00307337T patent/ATE299530T1/de not_active IP Right Cessation
- 2000-08-25 EP EP00307337A patent/EP1078984B1/de not_active Expired - Lifetime
- 2000-08-25 DE DE60021234T patent/DE60021234T2/de not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| EP1078984B1 (de) | 2005-07-13 |
| GB0021086D0 (en) | 2000-10-11 |
| DE60021234T2 (de) | 2006-04-27 |
| GB9920194D0 (en) | 1999-10-27 |
| DE60021234D1 (de) | 2005-08-18 |
| GB2353530B (en) | 2001-06-27 |
| EP1078984A1 (de) | 2001-02-28 |
| GB2353530A (en) | 2001-02-28 |
| US6479264B1 (en) | 2002-11-12 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |