ATE360096T1 - Reversibel modifizierte thermostabile enzyme für die in vitro-synthese und -amplifizierung von dna - Google Patents
Reversibel modifizierte thermostabile enzyme für die in vitro-synthese und -amplifizierung von dnaInfo
- Publication number
- ATE360096T1 ATE360096T1 AT02026712T AT02026712T ATE360096T1 AT E360096 T1 ATE360096 T1 AT E360096T1 AT 02026712 T AT02026712 T AT 02026712T AT 02026712 T AT02026712 T AT 02026712T AT E360096 T1 ATE360096 T1 AT E360096T1
- Authority
- AT
- Austria
- Prior art keywords
- modified thermostable
- activity
- enzyme
- thermostable enzyme
- amplification
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/99—Enzyme inactivation by chemical treatment
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- General Chemical & Material Sciences (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP01128725 | 2001-12-03 | ||
| US10/317,715 US7378262B2 (en) | 2001-12-03 | 2002-12-11 | Reversibly modified thermostable enzymes for DNA synthesis and amplification in vitro |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE360096T1 true ATE360096T1 (de) | 2007-05-15 |
Family
ID=32963733
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT02026712T ATE360096T1 (de) | 2001-12-03 | 2002-11-30 | Reversibel modifizierte thermostabile enzyme für die in vitro-synthese und -amplifizierung von dna |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US7378262B2 (de) |
| EP (1) | EP1319719B8 (de) |
| JP (1) | JP3969581B2 (de) |
| AT (1) | ATE360096T1 (de) |
| CA (1) | CA2409775C (de) |
| DE (1) | DE60219579T2 (de) |
| ES (1) | ES2284769T3 (de) |
Families Citing this family (49)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2004203649B2 (en) | 2003-08-12 | 2006-01-12 | F. Hoffmann-La Roche Ag | Thermostable Taq polymerase fragment |
| GB0502010D0 (en) * | 2005-02-01 | 2005-03-09 | Enigma Diagnostics Ltd | Biochemical reagents and their uses |
| AU2006269282B2 (en) * | 2005-07-07 | 2013-10-24 | Quanta Biosciences, Inc. | Compositions and methods for increasing amplification efficiency |
| US8962293B2 (en) | 2006-10-18 | 2015-02-24 | Roche Molecular Systems, Inc. | DNA polymerases and related methods |
| CN101050453B (zh) * | 2007-04-04 | 2010-06-30 | 无锡中德美联生物技术有限公司 | 一种热启动耐高温dna聚合酶及其制备方法 |
| EP2644708B1 (de) | 2008-04-30 | 2014-12-17 | Integrated Dna Technologies, Inc. | Rnase-h-basierte assays unter verwendung von modifizierten rna monomeren |
| WO2011157432A1 (en) | 2010-06-18 | 2011-12-22 | Roche Diagnostics Gmbh | Dna polymerases with increased 3'-mismatch discrimination |
| EP2582806B1 (de) | 2010-06-18 | 2015-12-16 | Roche Diagnostics GmbH | Dna-polymerasen mit erhöhter unterscheidung von 3'-diskrepanz |
| ES2536252T3 (es) | 2010-06-18 | 2015-05-21 | F. Hoffmann-La Roche Ag | ADN polimerasas con diferenciación de desapareamientos 3' aumentada |
| CA2802239C (en) | 2010-06-18 | 2016-08-23 | F. Hoffmann-La Roche Ag | Dna polymerases with increased 3'-mismatch discrimination |
| US8759062B2 (en) | 2010-06-18 | 2014-06-24 | Roche Molecular Systems, Inc. | DNA polymerases with increased 3′- mismatch discrimination |
| JP5926248B2 (ja) | 2010-06-18 | 2016-05-25 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 増大した３’末端ミスマッチ識別能を有するｄｎａポリメラーゼ |
| WO2011157434A1 (en) | 2010-06-18 | 2011-12-22 | Roche Diagnostics Gmbh | Dna polymerases with increased 3'-mismatch discrimination |
| WO2011157436A1 (en) | 2010-06-18 | 2011-12-22 | Roche Diagnostics Gmbh | Dna polymerases with increased 3'-mismatch discrimination |
| WO2012019168A2 (en) | 2010-08-06 | 2012-02-09 | Moderna Therapeutics, Inc. | Engineered nucleic acids and methods of use thereof |
| EP2625189B1 (de) | 2010-10-01 | 2018-06-27 | ModernaTX, Inc. | Manipulierte nukleinsäuren und anwendungsverfahren dafür |
| US8765435B2 (en) | 2011-02-15 | 2014-07-01 | Roche Molecular Systems, Inc. | DNA polymerases with increased 3′-mismatch discrimination |
| AU2012236099A1 (en) | 2011-03-31 | 2013-10-03 | Moderna Therapeutics, Inc. | Delivery and formulation of engineered nucleic acids |
| ES2561885T3 (es) | 2011-04-11 | 2016-03-01 | F. Hoffmann-La Roche Ag | ADN polimerasas de actividad mejorada |
| CA2839964C (en) | 2011-07-28 | 2019-03-12 | F. Hoffmann-La Roche Ag | Dna polymerases with improved activity |
| US9464124B2 (en) | 2011-09-12 | 2016-10-11 | Moderna Therapeutics, Inc. | Engineered nucleic acids and methods of use thereof |
| DE19216461T1 (de) | 2011-10-03 | 2021-10-07 | Modernatx, Inc. | Modifizierte nukleoside, nukleotide und nukleinsäuren und verwendungen davon |
| ES2569723T3 (es) | 2011-12-08 | 2016-05-12 | F. Hoffmann-La Roche Ag | Polimerasas de DNA con actividad mejorada |
| CN103998603B (zh) | 2011-12-08 | 2016-05-25 | 霍夫曼-拉罗奇有限公司 | 具有改进活性的dna聚合酶 |
| ES2668448T3 (es) | 2011-12-08 | 2018-05-18 | F. Hoffmann-La Roche Ag | ADN polimerasas con actividad mejorada |
| CA3018046A1 (en) | 2011-12-16 | 2013-06-20 | Moderna Therapeutics, Inc. | Modified nucleoside, nucleotide, and nucleic acid compositions |
| US9254311B2 (en) | 2012-04-02 | 2016-02-09 | Moderna Therapeutics, Inc. | Modified polynucleotides for the production of proteins |
| WO2013151665A2 (en) | 2012-04-02 | 2013-10-10 | modeRNA Therapeutics | Modified polynucleotides for the production of proteins associated with human disease |
| US9283287B2 (en) | 2012-04-02 | 2016-03-15 | Moderna Therapeutics, Inc. | Modified polynucleotides for the production of nuclear proteins |
| US9572897B2 (en) | 2012-04-02 | 2017-02-21 | Modernatx, Inc. | Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins |
| PL2922554T3 (pl) | 2012-11-26 | 2022-06-20 | Modernatx, Inc. | Na zmodyfikowany na końcach |
| US8980864B2 (en) | 2013-03-15 | 2015-03-17 | Moderna Therapeutics, Inc. | Compositions and methods of altering cholesterol levels |
| EP3041934A1 (de) | 2013-09-03 | 2016-07-13 | Moderna Therapeutics, Inc. | Chimäre polynukleotide |
| EP3052521A1 (de) | 2013-10-03 | 2016-08-10 | Moderna Therapeutics, Inc. | Polynukleotide zur codierung von low-density-lipoprotein-rezeptor |
| US20170204152A1 (en) | 2014-07-16 | 2017-07-20 | Moderna Therapeutics, Inc. | Chimeric polynucleotides |
| GB201414745D0 (en) | 2014-08-19 | 2014-10-01 | Articzymes As | Exonucleases |
| PL3394093T3 (pl) | 2015-12-23 | 2022-05-16 | Modernatx, Inc. | Metody stosowania polinukleotydów kodujących ligand ox40 |
| MA43587A (fr) | 2016-01-10 | 2018-11-14 | Modernatx Inc | Arnm thérapeutiques codant pour des anticorps anti-ctla-4 |
| JP7194594B2 (ja) | 2016-05-18 | 2022-12-22 | モデルナティエックス インコーポレイテッド | 免疫調節ポリペプチドをコードするｍＲＮＡの組み合わせ及びその使用 |
| SI3458083T1 (sl) | 2016-05-18 | 2023-03-31 | Modernatx, Inc. | Polinukleotidi, ki kodirajo interlevkin-12 (IL12) in njihova uporaba |
| US20200131498A1 (en) | 2017-06-14 | 2020-04-30 | Modernatx, Inc. | Polynucleotides encoding methylmalonyl-coa mutase |
| CN107312762B (zh) * | 2017-06-23 | 2020-08-11 | 苏州点晶生物科技有限公司 | 一种化学修饰ung酶及其修饰方法和应用 |
| US11739306B2 (en) | 2018-09-13 | 2023-08-29 | Roche Molecular Systems, Inc. | Mutant DNA polymerase(s) with improved strand displacement ability |
| CN113454238B (zh) | 2019-01-25 | 2025-02-11 | 哈佛大学校长及研究员协会 | 用于合成核酸的组合物和方法 |
| EP4073246A4 (de) | 2019-12-12 | 2023-12-06 | President And Fellows Of Harvard College | Zusammensetzungen und verfahren zur lichtgerichteten biomolekularen barcodierung |
| JP2021153393A (ja) * | 2020-03-25 | 2021-10-07 | 東洋紡株式会社 | 核酸増幅反応用組成物 |
| CA3176545A1 (en) | 2020-04-22 | 2021-10-28 | President And Fellows Of Harvard College | Isothermal methods, compositions, kits, and systems for detecting nucleic acids |
| EP4225946A4 (de) | 2020-10-06 | 2024-10-30 | President And Fellows Of Harvard College | Verfahren, zusammensetzungen, kits und systeme für signal-on-nachweis von neutralisierungszielen |
| EP4729632A1 (de) | 2024-10-18 | 2026-04-22 | AskBio Inc. | Verfahren zur synthese von dna |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5338671A (en) * | 1992-10-07 | 1994-08-16 | Eastman Kodak Company | DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody |
| US5436149A (en) * | 1993-02-19 | 1995-07-25 | Barnes; Wayne M. | Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension |
| US6410277B1 (en) * | 1993-02-19 | 2002-06-25 | Takara Shuzo Co., Ltd. | DNA polymersases with enhanced length of primer extension |
| US5556772A (en) * | 1993-12-08 | 1996-09-17 | Stratagene | Polymerase compositions and uses thereof |
| US5512462A (en) * | 1994-02-25 | 1996-04-30 | Hoffmann-La Roche Inc. | Methods and reagents for the polymerase chain reaction amplification of long DNA sequences |
| CA2214263A1 (en) * | 1995-03-07 | 1996-09-12 | Biomolecular Assays, Inc. | Pressure cycling reactor |
| CA2176193A1 (en) * | 1995-05-22 | 1996-11-23 | John Wesley Backus | Methods for polymerase chain reaction preamplification sterilization by exonucleases in the presence of phosphorothioated primers |
| US5773258A (en) * | 1995-08-25 | 1998-06-30 | Roche Molecular Systems, Inc. | Nucleic acid amplification using a reversibly inactivated thermostable enzyme |
| GB9920194D0 (en) * | 1999-08-27 | 1999-10-27 | Advanced Biotech Ltd | A heat-stable thermostable DNA polymerase for use in nucleic acid amplification |
| ATE286981T1 (de) * | 1999-09-28 | 2005-01-15 | Roche Diagnostics Gmbh | Thermostabiles enzym welches die genauigkeit thermostabiler dna polymerasen erhöht - zur verbesserung der nucleinsäuresynthese und in vitro amplifikation |
| US20030049614A1 (en) * | 1999-10-06 | 2003-03-13 | Holly Hurlbut Hogrefe | Compositions for dna amplification, synthesis, and mutagenesis |
-
2002
- 2002-11-28 CA CA2409775A patent/CA2409775C/en not_active Expired - Lifetime
- 2002-11-30 AT AT02026712T patent/ATE360096T1/de active
- 2002-11-30 EP EP02026712A patent/EP1319719B8/de not_active Expired - Lifetime
- 2002-11-30 ES ES02026712T patent/ES2284769T3/es not_active Expired - Lifetime
- 2002-11-30 DE DE60219579T patent/DE60219579T2/de not_active Expired - Lifetime
- 2002-12-03 JP JP2002351736A patent/JP3969581B2/ja not_active Expired - Lifetime
- 2002-12-11 US US10/317,715 patent/US7378262B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| US20040115639A1 (en) | 2004-06-17 |
| US7378262B2 (en) | 2008-05-27 |
| DE60219579D1 (de) | 2007-05-31 |
| JP3969581B2 (ja) | 2007-09-05 |
| EP1319719B8 (de) | 2008-08-27 |
| ES2284769T3 (es) | 2007-11-16 |
| JP2003199592A (ja) | 2003-07-15 |
| EP1319719B1 (de) | 2007-04-18 |
| EP1319719A1 (de) | 2003-06-18 |
| CA2409775A1 (en) | 2003-06-03 |
| DE60219579T2 (de) | 2008-01-03 |
| CA2409775C (en) | 2010-07-13 |
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| UEP | Publication of translation of european patent specification |
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