ATE310816T1 - Verfahren zur bestimmung einer genfunktion - Google Patents

Verfahren zur bestimmung einer genfunktion

Info

Publication number
ATE310816T1
ATE310816T1 AT01922516T AT01922516T ATE310816T1 AT E310816 T1 ATE310816 T1 AT E310816T1 AT 01922516 T AT01922516 T AT 01922516T AT 01922516 T AT01922516 T AT 01922516T AT E310816 T1 ATE310816 T1 AT E310816T1
Authority
AT
Austria
Prior art keywords
gene function
transformation
determining
under
function
Prior art date
Application number
AT01922516T
Other languages
English (en)
Inventor
Pamela L Sharpe
Vasantha Nagarajan
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Application granted granted Critical
Publication of ATE310816T1 publication Critical patent/ATE310816T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1079—Screening libraries by altering the phenotype or phenotypic trait of the host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
AT01922516T 2000-03-23 2001-03-21 Verfahren zur bestimmung einer genfunktion ATE310816T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US19156100P 2000-03-23 2000-03-23
PCT/US2001/009003 WO2001071040A2 (en) 2000-03-23 2001-03-21 Method for determination of gene function

Publications (1)

Publication Number Publication Date
ATE310816T1 true ATE310816T1 (de) 2005-12-15

Family

ID=22705975

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01922516T ATE310816T1 (de) 2000-03-23 2001-03-21 Verfahren zur bestimmung einer genfunktion

Country Status (7)

Country Link
US (2) US6673567B2 (de)
EP (1) EP1276903B1 (de)
JP (1) JP2003533981A (de)
AT (1) ATE310816T1 (de)
CA (1) CA2403259A1 (de)
DE (1) DE60115225D1 (de)
WO (1) WO2001071040A2 (de)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6673567B2 (en) * 2000-03-23 2004-01-06 E. I. Du Pont De Nemours And Company Method of determination of gene function
WO2002000916A2 (en) * 2000-06-28 2002-01-03 California Institute Of Technology Methods for identifying an essential gene in a prokaryotic microorganism
DE10109996A1 (de) * 2001-03-01 2002-09-05 Basf Ag Verfahren zur Veränderung des Genoms von gram-positiven Bakterien mit einem neuen konditional negativ dominanten Markergen
CN1596306A (zh) * 2001-03-07 2005-03-16 荧合金股份有限公司 用于将dna导入靶细胞的筛选方法
US20050181509A1 (en) * 2004-02-12 2005-08-18 The Penn State Research Foundation Dual selection based, targeted gene disruption method for fungi and fungus-like organisms
DK1781785T3 (da) * 2004-07-20 2011-02-14 Novozymes Inc Fremgangsmåde til fremstilling af mutant-polynukleotider
AU2006303957A1 (en) * 2005-10-12 2007-04-26 Synthetic Genomics, Inc. Minimal bacterial genome
US7700328B2 (en) 2006-06-07 2010-04-20 E.I. Du Pont De Nemours And Company Method for producing an L-tyrosine over-producing bacterial strain
WO2008040387A1 (en) 2006-10-03 2008-04-10 Metabolic Explorer Process for chromosomal integration and dna sequence replacement in clostridia
TR201802323T4 (tr) 2007-12-11 2018-03-21 Univ North Carolina Chapel Hill Polipürin yolu modifiye edilmiş retroviral vektörler.
BRPI1010679B1 (pt) * 2009-05-19 2020-09-24 Bioproperties Pty Ltd Cepa de vacina de mycoplasma hyopneumoniae sensível à temperatura e aplicações da mesma, composiçao de vacina, método de preparaçao e uso da mesma
JP6067204B2 (ja) * 2010-04-20 2017-01-25 三菱レイヨン株式会社 ロドコッカス細菌のためのランダムゲノム挿入及び欠失ツール
CN108728477B (zh) * 2017-04-24 2022-02-22 华东理工大学 一种高效的转座突变系统及构建方法
CN113150995A (zh) * 2021-04-22 2021-07-23 贵州安康医学检验中心有限公司 一种用于转运过程中的细菌转运保藏培养基及其配制方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5580734A (en) * 1990-07-13 1996-12-03 Transkaryotic Therapies, Inc. Method of producing a physical map contigous DNA sequences
EP1036183B1 (de) 1997-02-20 2007-10-03 The Johns Hopkins University School Of Medicine Mutationen in atp-abhängigen transpositionsproteinen die die zielortspezifität reduzieren
AU3196099A (en) 1998-03-27 1999-10-18 President And Fellows Of Harvard College Systematic identification of essential genes by (in vitro) transposon mutagenesis
EP1161551A2 (de) 1999-03-17 2001-12-12 Paradigm Genetics Inc. Methoden und materialien zur schnellen und in einer grossen ausbeute resultierenden herstellung von "knock-out" genbanken in einem organismus
US6673567B2 (en) * 2000-03-23 2004-01-06 E. I. Du Pont De Nemours And Company Method of determination of gene function

Also Published As

Publication number Publication date
JP2003533981A (ja) 2003-11-18
WO2001071040A2 (en) 2001-09-27
WO2001071040A3 (en) 2002-11-28
CA2403259A1 (en) 2001-09-27
DE60115225D1 (de) 2005-12-29
US20050053929A1 (en) 2005-03-10
US20030215799A1 (en) 2003-11-20
EP1276903B1 (de) 2005-11-23
US6673567B2 (en) 2004-01-06
EP1276903A2 (de) 2003-01-22

Similar Documents

Publication Publication Date Title
Li et al. Volatile compounds from beneficial rhizobacteria Bacillus spp. promote periodic lateral root development in Arabidopsis
DE60115225D1 (de) Verfahren zur bestimmung einer genfunktion
Croll et al. Nonself vegetative fusion and genetic exchange in the arbuscular mycorrhizal fungus Glomus intraradices
ATE414782T1 (de) Verfahren zur herstellung von polypeptiden in mutierten aspergillus zellen
Arai Foxing caused by fungi: twenty-five years of study
WO2003010304A3 (de) Verfahren zur herstellung isolierter zellkulturen, kulturmedium zur kultivierung von zellkulturen und zellkultur
WO2002010438A3 (en) Serial analysis of transcript expression using long tags
ATE87032T1 (de) Verbessertes verfahren zur transformation von pflanzlichen protoplasten.
EP1304628A3 (de) Verfahren und Gerät zur Auswahl und zur Reproduktion von Information
WO2000053804A3 (en) Genetic screening
NZ597400A (en) Methods and Materials Using Signaling Probes
NZ538852A (en) Selection and isolation of living cells using mRNA-binding probes
ATE246251T1 (de) Verfahren zur herstellung von polypeptiden in surfaktinmutanten von bacillus zellen
WO2001075161A3 (en) Genetic markers for improved meat characteristics in animals
WO2001021760A3 (en) Cell culture spinner flasks
DK1078081T3 (da) Fremgangsmåde til fremstilling af heterologe polypeptider i trichothecen deficiente filamentöse svampe mutantceller
Rolfe et al. Interaction between a non-nodulating and an ineffective mutant of Rhizobium trifolli resulting in effective (nitrogen-fixing) nodulation
Martínez‐García et al. Ammonia‐oxidizing Crenarchaeota and nitrification inside the tissue of a colonial ascidian
CN101712984A (zh) 鉴别香菇菌种241和241-4的分子特异性标记引物及检测方法
CN106282396B (zh) 鉴定梯棱羊肚菌交配型的方法和特异引物对
WO2001085909A3 (en) Development of plant based biological sensors and novel disease resistance strategies
AU2002257284A1 (en) Pna probes, probe sets, methods and kits pertaining to the detection of candida
Lee et al. Phylogenetic relationships of Holarctic Teleiodini (Lepidoptera: Gelechiidae) based on analysis of morphological and molecular data
NO20006051L (no) Fremgangsmåte for fremstilling av dyrkning av celleaggregater referert til som primmorfer fra dissosierte celler fra svamper, koraller og andre invertebrater, samt anvendelse av disse
Green et al. Multiple human TK chromosomes in human-mouse somatic cell hybrids

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties