ATE310816T1 - Verfahren zur bestimmung einer genfunktion - Google Patents

Verfahren zur bestimmung einer genfunktion

Info

Publication number
ATE310816T1
ATE310816T1 AT01922516T AT01922516T ATE310816T1 AT E310816 T1 ATE310816 T1 AT E310816T1 AT 01922516 T AT01922516 T AT 01922516T AT 01922516 T AT01922516 T AT 01922516T AT E310816 T1 ATE310816 T1 AT E310816T1
Authority
AT
Austria
Prior art keywords
gene function
transformation
determining
under
function
Prior art date
Application number
AT01922516T
Other languages
English (en)
Inventor
Pamela L Sharpe
Vasantha Nagarajan
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Application granted granted Critical
Publication of ATE310816T1 publication Critical patent/ATE310816T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1079—Screening libraries by altering the phenotype or phenotypic trait of the host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
AT01922516T 2000-03-23 2001-03-21 Verfahren zur bestimmung einer genfunktion ATE310816T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US19156100P 2000-03-23 2000-03-23
PCT/US2001/009003 WO2001071040A2 (en) 2000-03-23 2001-03-21 Method for determination of gene function

Publications (1)

Publication Number Publication Date
ATE310816T1 true ATE310816T1 (de) 2005-12-15

Family

ID=22705975

Family Applications (1)

Application Number Title Priority Date Filing Date
AT01922516T ATE310816T1 (de) 2000-03-23 2001-03-21 Verfahren zur bestimmung einer genfunktion

Country Status (7)

Country Link
US (2) US6673567B2 (de)
EP (1) EP1276903B1 (de)
JP (1) JP2003533981A (de)
AT (1) ATE310816T1 (de)
CA (1) CA2403259A1 (de)
DE (1) DE60115225D1 (de)
WO (1) WO2001071040A2 (de)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6673567B2 (en) * 2000-03-23 2004-01-06 E. I. Du Pont De Nemours And Company Method of determination of gene function
US6991900B2 (en) * 2000-06-28 2006-01-31 California Institute Of Technology Methods for identifying an essential gene in a prokaryotic microorganism
DE10109996A1 (de) * 2001-03-01 2002-09-05 Basf Ag Verfahren zur Veränderung des Genoms von gram-positiven Bakterien mit einem neuen konditional negativ dominanten Markergen
JP2004537280A (ja) * 2001-03-07 2004-12-16 ゼノジェン コーポレイション 標的細胞中へのｄｎａの導入についてのスクリーニング方法
US20050181509A1 (en) * 2004-02-12 2005-08-18 The Penn State Research Foundation Dual selection based, targeted gene disruption method for fungi and fungus-like organisms
WO2006017371A1 (en) * 2004-07-20 2006-02-16 Novozymes, Inc. Methods of producing mutant polynucleotides
AU2006303957A1 (en) * 2005-10-12 2007-04-26 Synthetic Genomics, Inc. Minimal bacterial genome
US7700328B2 (en) 2006-06-07 2010-04-20 E.I. Du Pont De Nemours And Company Method for producing an L-tyrosine over-producing bacterial strain
MX2009003605A (es) * 2006-10-03 2009-04-22 Metabolic Explorer Sa Procedimiento para integracion cromosomica y reemplazo de secuencia de adn en clostridia.
LT2222861T (lt) 2007-12-11 2018-03-26 The University Of North Carolina At Chapel Hill Retrovirusiniai vektoriai modifikuoti polipurino atkarpa
PE20120909A1 (es) * 2009-05-19 2012-08-19 Bioproperties Pty Ltd Cepa de vacuna de mycoplasma hyopneumoniae sensible a la temperatura y usos de la misma
JP6067204B2 (ja) * 2010-04-20 2017-01-25 三菱レイヨン株式会社 ロドコッカス細菌のためのランダムゲノム挿入及び欠失ツール
CN108728477B (zh) * 2017-04-24 2022-02-22 华东理工大学 一种高效的转座突变系统及构建方法
CN113150995A (zh) * 2021-04-22 2021-07-23 贵州安康医学检验中心有限公司 一种用于转运过程中的细菌转运保藏培养基及其配制方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5580734A (en) * 1990-07-13 1996-12-03 Transkaryotic Therapies, Inc. Method of producing a physical map contigous DNA sequences
ATE374821T1 (de) 1997-02-20 2007-10-15 Univ Johns Hopkins Med Mutationen in atp-abhängigen transpositionsproteinen die die zielortspezifität reduzieren
AU3196099A (en) 1998-03-27 1999-10-18 President And Fellows Of Harvard College Systematic identification of essential genes by (in vitro) transposon mutagenesis
JP2003502015A (ja) 1999-03-17 2003-01-21 パラダイム ジェネティックス、 インコーポレイテッド 生物中における遺伝子ノックアウトライブラリの迅速かつ大量産生のための方法類および素材類
US6673567B2 (en) * 2000-03-23 2004-01-06 E. I. Du Pont De Nemours And Company Method of determination of gene function

Also Published As

Publication number Publication date
US20050053929A1 (en) 2005-03-10
JP2003533981A (ja) 2003-11-18
EP1276903A2 (de) 2003-01-22
EP1276903B1 (de) 2005-11-23
DE60115225D1 (de) 2005-12-29
US20030215799A1 (en) 2003-11-20
WO2001071040A3 (en) 2002-11-28
CA2403259A1 (en) 2001-09-27
WO2001071040A2 (en) 2001-09-27
US6673567B2 (en) 2004-01-06

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