ATE312942T1 - Amplifizierung unter verwendung von modifizierten primern - Google Patents
Amplifizierung unter verwendung von modifizierten primernInfo
- Publication number
- ATE312942T1 ATE312942T1 AT01125022T AT01125022T ATE312942T1 AT E312942 T1 ATE312942 T1 AT E312942T1 AT 01125022 T AT01125022 T AT 01125022T AT 01125022 T AT01125022 T AT 01125022T AT E312942 T1 ATE312942 T1 AT E312942T1
- Authority
- AT
- Austria
- Prior art keywords
- amplification
- modified primers
- primers
- carried out
- amplifications carried
- Prior art date
Links
- 230000003321 amplification Effects 0.000 title abstract 4
- 238000003199 nucleic acid amplification method Methods 0.000 title abstract 4
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 1
- 239000000539 dimer Substances 0.000 abstract 1
- 150000007523 nucleic acids Chemical group 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Dental Preparations (AREA)
- Adhesives Or Adhesive Processes (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US24318200P | 2000-10-25 | 2000-10-25 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE312942T1 true ATE312942T1 (de) | 2005-12-15 |
Family
ID=22917659
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT01125022T ATE312942T1 (de) | 2000-10-25 | 2001-10-20 | Amplifizierung unter verwendung von modifizierten primern |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US6794142B2 (de) |
| EP (1) | EP1201768B1 (de) |
| JP (1) | JP3737734B2 (de) |
| AT (1) | ATE312942T1 (de) |
| CA (1) | CA2359838C (de) |
| DE (1) | DE60115811T2 (de) |
| ES (1) | ES2254306T3 (de) |
Families Citing this family (41)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2360011A1 (en) | 1999-01-05 | 2000-07-13 | Trustees Of Boston University | Ordered gene assembly |
| US7435562B2 (en) | 2000-07-21 | 2008-10-14 | Modular Genetics, Inc. | Modular vector systems |
| US9261460B2 (en) | 2002-03-12 | 2016-02-16 | Enzo Life Sciences, Inc. | Real-time nucleic acid detection processes and compositions |
| US20040161741A1 (en) | 2001-06-30 | 2004-08-19 | Elazar Rabani | Novel compositions and processes for analyte detection, quantification and amplification |
| US9777312B2 (en) | 2001-06-30 | 2017-10-03 | Enzo Life Sciences, Inc. | Dual polarity analysis of nucleic acids |
| US9353405B2 (en) | 2002-03-12 | 2016-05-31 | Enzo Life Sciences, Inc. | Optimized real time nucleic acid detection processes |
| WO2006053131A1 (en) | 2004-11-11 | 2006-05-18 | Modular Genetics, Inc. | Ladder assembly and system for generating diversity |
| US20070218490A1 (en) * | 2006-03-15 | 2007-09-20 | Integrated Dna Technologies, Inc. | Nucleic acid monomers with 2'-chemical moieties |
| US7833716B2 (en) | 2006-06-06 | 2010-11-16 | Gen-Probe Incorporated | Tagged oligonucleotides and their use in nucleic acid amplification methods |
| US9045522B2 (en) | 2006-07-31 | 2015-06-02 | Wanli Bi | Nucleic acid amplification using a reversibly modified oligonucleotide |
| CN106566855B (zh) * | 2006-07-31 | 2021-11-09 | 苏州新海生物科技股份有限公司 | 使用可逆修饰寡核苷酸扩增核酸 |
| US9328378B2 (en) * | 2006-07-31 | 2016-05-03 | Illumina Cambridge Limited | Method of library preparation avoiding the formation of adaptor dimers |
| US20090004662A1 (en) | 2007-06-18 | 2009-01-01 | Applera Corporation | Method and compositions for nucleic acid amplification |
| CA2691942C (en) | 2007-07-03 | 2017-12-05 | Genaphora Ltd. | Methods and kits using chimeric probes for reducing or eliminating formation of artifacts and non-specific amplification products |
| DE102008005667A1 (de) | 2008-01-19 | 2009-07-30 | Olfert Landt | Basenmodifizierte Primeroligomere für die Multiplex-RT-PCR |
| EP2388322B1 (de) | 2009-01-15 | 2017-10-25 | Hokkaido Mitsui Chemicals, Inc. | Enzymatische zubereitung mit thermostabiler dna-polmerase, herstellungsverfahren dafür und verfahren für den nachweis eines analytorganismus |
| US20110003301A1 (en) * | 2009-05-08 | 2011-01-06 | Life Technologies Corporation | Methods for detecting genetic variations in dna samples |
| WO2012021493A2 (en) | 2010-08-13 | 2012-02-16 | Envirologix Inc. | Compositions and methods for quantifying a nucleic acid sequence in a sample |
| US9115394B2 (en) | 2011-12-22 | 2015-08-25 | Roche Molecular Systems, Inc. | Methods and reagents for reducing non-specific amplification |
| EP2820129A1 (de) | 2012-03-02 | 2015-01-07 | Sequenom, Inc. | Verfahren und prozesse zur nichtinvasiven beurteilung genetischer variationen |
| RU2601129C2 (ru) * | 2012-04-09 | 2016-10-27 | Инвайролоджикс Инк. | Композиции и способы для количественного определения последовательности нуклеиновой кислоты в образце |
| JP6450308B2 (ja) | 2012-06-14 | 2019-01-09 | ライフ テクノロジーズ コーポレーション | ポリメラーゼ連鎖反応（ｐｃｒ）のための新規の組成物、方法、およびキット |
| EP2722399A1 (de) | 2012-10-18 | 2014-04-23 | Roche Diagniostics GmbH | Verfahren zur Vermeidung von Produkten mit hohem Molekulargewicht während einer Amplifikation |
| US11060145B2 (en) * | 2013-03-13 | 2021-07-13 | Sequenom, Inc. | Methods and compositions for identifying presence or absence of hypermethylation or hypomethylation locus |
| WO2015035108A1 (en) | 2013-09-05 | 2015-03-12 | The Jackson Laboratory | Compositions for rna-chromatin interaction analysis and uses thereof |
| EP3736344A1 (de) | 2014-03-13 | 2020-11-11 | Sequenom, Inc. | Verfahren und prozesse zur nichtinvasiven beurteilung genetischer variationen |
| EP3748011B1 (de) | 2014-04-22 | 2024-11-13 | Envirologix Inc. | Zusammensetzungen und verfahren zur verbesserung und/oder vorhersage der dna-amplifikation |
| CN107208137A (zh) | 2014-10-20 | 2017-09-26 | 龙公司 | 检测rna病毒的组合物和方法 |
| CN107406884A (zh) * | 2015-03-06 | 2017-11-28 | 西格马-奥尔德里奇有限责任公司 | 核酸扩增和文库制备 |
| WO2017121836A1 (en) | 2016-01-15 | 2017-07-20 | Thermo Fisher Scientific Baltics Uab | Thermophilic dna polymerase mutants |
| WO2017169119A1 (ja) * | 2016-03-30 | 2017-10-05 | 大研医器株式会社 | 変異プライマーの設計方法 |
| JP6681804B2 (ja) * | 2016-03-30 | 2020-04-15 | 大研医器株式会社 | 変異プライマーの設計方法 |
| GB201611469D0 (en) | 2016-06-30 | 2016-08-17 | Lumiradx Tech Ltd | Improvements in or relating to nucleic acid amplification processes |
| US10844425B2 (en) | 2017-03-24 | 2020-11-24 | Gen-Probe Incorporated | Compositions and methods for detecting or quantifying parainfluenza virus |
| EP3645710A1 (de) | 2017-06-26 | 2020-05-06 | Thermo Fisher Scientific Baltics Uab | Thermophile dna-polymerase-mutanten |
| GB2569965A (en) | 2018-01-04 | 2019-07-10 | Lumiradx Uk Ltd | Improvements in or relating to amplification of nucleic acids |
| WO2020013058A1 (ja) | 2018-07-13 | 2020-01-16 | タカラバイオ株式会社 | Ｒｎａからの核酸増幅反応に適したｄｎａポリメラーゼ変異体 |
| JP7684694B2 (ja) * | 2019-06-06 | 2025-05-28 | ザ・リージエンツ・オブ・ザ・ユニバーシテイ・オブ・コロラド、ア・ボデイー・コーポレイト | 粘着末端型ポリヌクレオチドの直接合成のための新規のシステム、方法、および組成物 |
| EP4450634A4 (de) | 2021-12-15 | 2025-12-24 | Nanjing Genscript Biotech Co Ltd | Verfahren zur erkennung einer integrationsstelle |
| CN114410836B (zh) * | 2021-12-17 | 2024-03-01 | 上海交通大学医学院附属仁济医院 | 一种集样本处理、核酸提取及多重恒温扩增一体化的检测人细小病毒b19的试剂盒和方法 |
| US20240052417A1 (en) * | 2022-08-12 | 2024-02-15 | Imam Abdulrahman Bin Faisal University | Method for early determination of gender by multiplex pcr detecting four genes |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5496938A (en) * | 1990-06-11 | 1996-03-05 | Nexstar Pharmaceuticals, Inc. | Nucleic acid ligands to HIV-RT and HIV-1 rev |
| JP2000514307A (ja) * | 1996-07-16 | 2000-10-31 | ジェン―プローブ・インコーポレーテッド | 増加した標的特異的ｔ▲下ｍ▼を持つ修飾オリゴヌクレオチドを用いた核酸配列の検出および増幅のための方法 |
| EP0866071B1 (de) * | 1997-03-20 | 2004-10-20 | F. Hoffmann-La Roche Ag | Modifizierte Primer |
| US7074895B2 (en) * | 1997-08-21 | 2006-07-11 | Quark Biotech, Inc. | Sequences characteristic of hypoxia-regulated gene transcription |
| GB9906328D0 (en) * | 1999-03-19 | 1999-05-12 | Zeneca Ltd | 2-Substituted RNA preparation |
-
2001
- 2001-10-20 DE DE60115811T patent/DE60115811T2/de not_active Expired - Lifetime
- 2001-10-20 ES ES01125022T patent/ES2254306T3/es not_active Expired - Lifetime
- 2001-10-20 EP EP01125022A patent/EP1201768B1/de not_active Expired - Lifetime
- 2001-10-20 AT AT01125022T patent/ATE312942T1/de active
- 2001-10-24 US US10/083,233 patent/US6794142B2/en not_active Expired - Lifetime
- 2001-10-24 CA CA002359838A patent/CA2359838C/en not_active Expired - Fee Related
- 2001-10-24 JP JP2001326463A patent/JP3737734B2/ja not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| EP1201768A3 (de) | 2004-02-11 |
| JP2002291490A (ja) | 2002-10-08 |
| DE60115811T2 (de) | 2006-08-03 |
| EP1201768A2 (de) | 2002-05-02 |
| CA2359838A1 (en) | 2002-04-25 |
| CA2359838C (en) | 2006-07-04 |
| US20030044817A1 (en) | 2003-03-06 |
| EP1201768B1 (de) | 2005-12-14 |
| ES2254306T3 (es) | 2006-06-16 |
| DE60115811D1 (de) | 2006-01-19 |
| US6794142B2 (en) | 2004-09-21 |
| JP3737734B2 (ja) | 2006-01-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| ATE312942T1 (de) | Amplifizierung unter verwendung von modifizierten primern | |
| ATE280177T1 (de) | Modifizierte primer | |
| EP2272952B8 (de) | Thermostabiler oder thermoactiver DNA-polymerase mit abgeschwächter 3'-5' Exonukleaseaktivität | |
| ATE364718T1 (de) | Verfahren zur vervielfältigung von nukleinsäure | |
| DK0408295T3 (da) | Fremgangsmåder til opformering af nukleinsyresekvenser | |
| FI103809B1 (fi) | In vitro -menetelmä templaattien tuottamiseksi DNA-sekventointia varten | |
| WO2004022770A3 (en) | Compositions and methods for synthesizing nucleic acids | |
| WO2004016767A3 (en) | Evolving new molecular function | |
| ATE362532T1 (de) | Verfahren zur amplifizierung einer nukleinsäuresequenz unter verwendung eines chimären primers | |
| EP1456409A4 (de) | Paralleles scoring von polymorphismen mittels amplifikation und fehlerkorrektur | |
| DE60009323D1 (de) | Methoden und zusammensetzungen zur linearen isothermalen amplifikation von polynukleotidsequenzen | |
| EP1256630A3 (de) | Selektive Polymerasekettenreaktion von DNA, deren Basensequenz vollständig unbekannt ist | |
| DK144886A (da) | Fremgangsmaade til multiplikation af en specifik nukleinsyresekvens | |
| ATE266737T1 (de) | Künstliche fehlhybridisierung | |
| DK0726324T3 (da) | Anvendelse af exonuklease og/eller glycosylase som supplementer til antipolymeraseantistof til forøgelse af specification i polymerasekædereaktion | |
| WO2001025483A3 (en) | Compositions for dna amplification, synthesis, and mutagenesis | |
| ATE440147T1 (de) | Erhíhung der spezifität von nukleinsäureamplifizierungen durch trägernukleinsäuren | |
| DE602004025624D1 (de) | Verfahren zur nukleinsäureamplifikation | |
| ATE455866T1 (de) | Verfahren zur unspezifischen amplifizierung nukleinsäuren mit niedriger komplexität | |
| ATE526418T1 (de) | Verbesserte zusammensetzungen zur in-vitro- amplifikation von nukleinsäuren | |
| ATE456669T1 (de) | Verfahren zur quantifizierung von nukleinsäuren |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification |
Ref document number: 1201768 Country of ref document: EP |