ATE314459T1 - COMPOSITIONS AND METHODS FOR PREVENTING MICROBIAL CONTAMINATION OF PLANT TISSUE CULTURE MEDIA - Google Patents
COMPOSITIONS AND METHODS FOR PREVENTING MICROBIAL CONTAMINATION OF PLANT TISSUE CULTURE MEDIAInfo
- Publication number
- ATE314459T1 ATE314459T1 AT96916997T AT96916997T ATE314459T1 AT E314459 T1 ATE314459 T1 AT E314459T1 AT 96916997 T AT96916997 T AT 96916997T AT 96916997 T AT96916997 T AT 96916997T AT E314459 T1 ATE314459 T1 AT E314459T1
- Authority
- AT
- Austria
- Prior art keywords
- plant
- tissue culture
- plant tissue
- methods
- culture medium
- Prior art date
Links
- 238000004161 plant tissue culture Methods 0.000 title abstract 4
- 239000003104 tissue culture media Substances 0.000 title abstract 4
- 230000000813 microbial effect Effects 0.000 title abstract 3
- 238000011109 contamination Methods 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 239000000203 mixture Substances 0.000 title abstract 2
- 239000013043 chemical agent Substances 0.000 abstract 3
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 abstract 2
- YIXJRHPUWRPCBB-UHFFFAOYSA-N magnesium nitrate Chemical compound [Mg+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O YIXJRHPUWRPCBB-UHFFFAOYSA-N 0.000 abstract 2
- 210000000056 organ Anatomy 0.000 abstract 2
- CHHHXKFHOYLYRE-UHFFFAOYSA-M 2,4-Hexadienoic acid, potassium salt (1:1), (2E,4E)- Chemical compound [K+].CC=CC=CC([O-])=O CHHHXKFHOYLYRE-UHFFFAOYSA-M 0.000 abstract 1
- 229940100555 2-methyl-4-isothiazolin-3-one Drugs 0.000 abstract 1
- 229940100484 5-chloro-2-methyl-4-isothiazolin-3-one Drugs 0.000 abstract 1
- DHNRXBZYEKSXIM-UHFFFAOYSA-N chloromethylisothiazolinone Chemical compound CN1SC(Cl)=CC1=O DHNRXBZYEKSXIM-UHFFFAOYSA-N 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 230000018109 developmental process Effects 0.000 abstract 1
- 230000035784 germination Effects 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 229910001629 magnesium chloride Inorganic materials 0.000 abstract 1
- 229960002337 magnesium chloride Drugs 0.000 abstract 1
- BEGLCMHJXHIJLR-UHFFFAOYSA-N methylisothiazolinone Chemical compound CN1SC=CC1=O BEGLCMHJXHIJLR-UHFFFAOYSA-N 0.000 abstract 1
- 235000010241 potassium sorbate Nutrition 0.000 abstract 1
- 239000004302 potassium sorbate Substances 0.000 abstract 1
- 229940069338 potassium sorbate Drugs 0.000 abstract 1
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical compound [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 abstract 1
- 235000010234 sodium benzoate Nutrition 0.000 abstract 1
- 239000004299 sodium benzoate Substances 0.000 abstract 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0018—Culture media for cell or tissue culture
- C12N5/0025—Culture media for plant cell or plant tissue culture
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Developmental Biology & Embryology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Botany (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Medicines Containing Plant Substances (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The present invention encompasses compositions and methods to reduce or prevent microbial growth in plant tissue culture media, comprising adding a chemical agent comprising methylchloroisothiazolinone, methylisothiazolinone, magnesium chloride and magnesium nitrate to a plant culture medium at a concentration that reduces or prevents microbial contamination of the plant tissue culture medium and that allows substantially normal germination of seeds or substantially normal growth or development of plants, plant organs, plant tissues or plant cells. The chemical agent may further comprise potassium sorbate or sodium benzoate, or both. The present invention further provides a kit for germinating plant seeds or culturing plants, plant organs, plant tissues or plant cells on a plant tissue culture medium comprising the chemical agent.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/460,703 US5750402A (en) | 1995-06-02 | 1995-06-02 | Compositions and methods to prevent microbial contamination of plant tissue culture media |
| PCT/US1996/008423 WO1996038542A1 (en) | 1995-06-02 | 1996-06-03 | Compositions and methods to prevent microbial contamination of plant tissue culture media |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE314459T1 true ATE314459T1 (en) | 2006-01-15 |
Family
ID=23829745
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT96916997T ATE314459T1 (en) | 1995-06-02 | 1996-06-03 | COMPOSITIONS AND METHODS FOR PREVENTING MICROBIAL CONTAMINATION OF PLANT TISSUE CULTURE MEDIA |
Country Status (16)
| Country | Link |
|---|---|
| US (1) | US5750402A (en) |
| EP (1) | EP0828820B1 (en) |
| JP (1) | JPH11506331A (en) |
| CN (1) | CN1193344A (en) |
| AT (1) | ATE314459T1 (en) |
| AU (1) | AU698103B2 (en) |
| BR (1) | BR9608621A (en) |
| CA (1) | CA2222935C (en) |
| DE (2) | DE69635658T2 (en) |
| ES (1) | ES2256860T3 (en) |
| HU (1) | HUP9900888A2 (en) |
| IL (1) | IL122373A (en) |
| NO (1) | NO975556L (en) |
| NZ (1) | NZ309512A (en) |
| PL (1) | PL323749A1 (en) |
| WO (1) | WO1996038542A1 (en) |
Families Citing this family (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5803014A (en) * | 1997-02-14 | 1998-09-08 | Plant Cell Technology, Inc. | Habitat media for ants and other invertebrates |
| AR039365A1 (en) * | 2002-11-08 | 2005-02-16 | Magri Federico German | A METHOD FOR THE PREPARATION OF A CONCENTRATED INOCULANT AND CONCENTRATED INOCULATING COMPOSITION OBTAINED WITH SUCH METHOD |
| US6841572B2 (en) * | 2003-02-20 | 2005-01-11 | H&I Agritech | Environmentally safe fungicide and bactericide formulations |
| EP1772055A1 (en) * | 2005-10-04 | 2007-04-11 | Rohm and Haas France SAS | Synergistic microbicidal compositions comprising a N-alkyl-1,2-benzoisothiazolin-3-one |
| US20090035340A1 (en) * | 2007-07-30 | 2009-02-05 | Kimberly-Clark Worldwide, Inc. | Preservative compositions for moist wipes |
| CN101255385B (en) * | 2008-04-10 | 2011-03-16 | 杨壮 | Cleaning agent and cleaning supplies comprising the same |
| WO2011017565A2 (en) * | 2009-08-07 | 2011-02-10 | Joule Unlimited, Inc. | Methods and compositions for controlling contamination growth in cell cultures |
| CN103155861A (en) * | 2011-12-10 | 2013-06-19 | 何寒 | Plant open-type issue culture seedling method |
| CN103881774B (en) * | 2012-12-23 | 2016-06-15 | 北京合创同盛科技有限公司 | A kind of additive stablizing liquid hydrocarbon oil |
| CN103329800A (en) * | 2013-06-17 | 2013-10-02 | 何寒 | Tissue culture medium without agar during plant open tissue culturing and preparation method of tissue culture medium |
| CN103734010A (en) * | 2013-12-30 | 2014-04-23 | 古田县恒春农业开发有限公司 | Mildewproof glue for tissue culture bottleneck and using method of mildewproof glue |
| CN105409780B (en) * | 2015-12-30 | 2019-03-29 | 玉溪市飞熊农业开发有限公司 | A kind of plant tissue culture culture medium and preparation method thereof without sterilizing |
| CN106745773A (en) * | 2016-12-31 | 2017-05-31 | 许伟琦 | The ecological treatment system and method and inorganic agent, conditioning agent of city domestic sewage, trade effluent containing high salt organic matter high |
| US12501898B2 (en) * | 2017-09-29 | 2025-12-23 | 0903608 B.C. Ltd. | Synergistic pesticidal compositions and methods for delivery of active ingredients |
| KR102753090B1 (en) * | 2017-12-21 | 2025-01-14 | 젠자임 코포레이션 | Method for improved removal of impurities during protein A chromatography |
| CN113286513A (en) | 2018-09-27 | 2021-08-20 | 0903608Bc有限公司 | Synergistic pesticide compositions and methods for delivering insecticidal active ingredients |
| CN109874669B (en) * | 2019-01-16 | 2022-04-19 | 南京农业大学 | Method for rapidly propagating aseptic lotus seedlings by stem walking |
| CN112753581A (en) * | 2021-02-04 | 2021-05-07 | 公孙国林 | Cultivation formula of polyploid plant of Dolicholepis of Africaceae |
| CN114600757B (en) * | 2022-03-25 | 2023-07-18 | 广东环境保护工程职业学院 | Seedling raising method for hovenia dulcis thunb |
| CN115428797B (en) * | 2022-09-28 | 2024-05-07 | 山东省农业科学院 | A method for sterilizing onion seeds and cultivating aseptic seedlings |
| KR102629108B1 (en) * | 2023-09-01 | 2024-01-25 | 김연준 | Planting Medium Antimicrobial Composition |
| KR102760371B1 (en) * | 2024-09-19 | 2025-02-03 | 농업회사법인 주식회사 파이토리서치 | Planting Medium Antimicrobial Composition |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3122432A (en) * | 1964-02-25 | Composition and method for preserving | ||
| US4325201A (en) * | 1967-03-09 | 1982-04-20 | Rohm And Haas Company | Seed treatment with 3-isothiazolones |
| US3452034A (en) * | 1967-03-09 | 1969-06-24 | American Cyanamid Co | Substituted 2-(1,3,4-thiadiazol-2-yl)-4(5)-nitroimidazoles |
| US4265899A (en) * | 1978-05-30 | 1981-05-05 | Rohm And Haas Company | Cosmetic formulation comprising 3-isothiazolones |
| US4454146A (en) * | 1982-05-14 | 1984-06-12 | Lever Brothers Company | Synergistic preservative compositions |
| US4555400A (en) * | 1982-05-14 | 1985-11-26 | Lever Brothers Company | Synergistic preservative compositions |
| US4540570A (en) * | 1982-05-14 | 1985-09-10 | Lever Brothers Company | Synergistic preservative compositions |
| US4499071A (en) * | 1982-05-14 | 1985-02-12 | Lever Brothers Company | Synergistic preservative compositions |
| US5028620A (en) * | 1988-09-15 | 1991-07-02 | Rohm And Haas Company | Biocide composition |
| US5100905A (en) * | 1988-09-15 | 1992-03-31 | Rohm And Haas Company | Method for inhibiting growth of bacteria, fungi or algae by treatment with biocide composition |
| JP2803336B2 (en) * | 1990-07-02 | 1998-09-24 | 松下電器産業株式会社 | Plant tissue culture method |
| JPH04311326A (en) * | 1990-12-26 | 1992-11-04 | Hokko Chem Ind Co Ltd | Method for culturing tissue of cyclamen |
-
1995
- 1995-06-02 US US08/460,703 patent/US5750402A/en not_active Expired - Lifetime
-
1996
- 1996-06-03 CN CN96196050A patent/CN1193344A/en active Pending
- 1996-06-03 AU AU59701/96A patent/AU698103B2/en not_active Expired
- 1996-06-03 CA CA2222935A patent/CA2222935C/en not_active Expired - Lifetime
- 1996-06-03 AT AT96916997T patent/ATE314459T1/en not_active IP Right Cessation
- 1996-06-03 PL PL96323749A patent/PL323749A1/en unknown
- 1996-06-03 IL IL12237396A patent/IL122373A/en not_active IP Right Cessation
- 1996-06-03 ES ES96916997T patent/ES2256860T3/en not_active Expired - Lifetime
- 1996-06-03 BR BR9608621A patent/BR9608621A/en not_active Application Discontinuation
- 1996-06-03 DE DE69635658T patent/DE69635658T2/en not_active Expired - Lifetime
- 1996-06-03 HU HU9900888A patent/HUP9900888A2/en unknown
- 1996-06-03 DE DE96916997T patent/DE96916997T1/en active Pending
- 1996-06-03 JP JP8536770A patent/JPH11506331A/en active Pending
- 1996-06-03 EP EP96916997A patent/EP0828820B1/en not_active Expired - Lifetime
- 1996-06-03 WO PCT/US1996/008423 patent/WO1996038542A1/en not_active Ceased
- 1996-06-03 NZ NZ309512A patent/NZ309512A/en unknown
-
1997
- 1997-12-02 NO NO975556A patent/NO975556L/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| DE96916997T1 (en) | 2005-07-14 |
| IL122373A (en) | 2000-12-06 |
| NO975556L (en) | 1998-01-26 |
| ES2256860T3 (en) | 2006-07-16 |
| CA2222935A1 (en) | 1996-12-05 |
| US5750402A (en) | 1998-05-12 |
| NO975556D0 (en) | 1997-12-02 |
| BR9608621A (en) | 1999-08-17 |
| AU698103B2 (en) | 1998-10-22 |
| PL323749A1 (en) | 1998-04-14 |
| CA2222935C (en) | 2012-02-07 |
| HUP9900888A2 (en) | 1999-07-28 |
| DE69635658T2 (en) | 2006-09-07 |
| WO1996038542A1 (en) | 1996-12-05 |
| IL122373A0 (en) | 1998-06-15 |
| CN1193344A (en) | 1998-09-16 |
| DE69635658D1 (en) | 2006-02-02 |
| JPH11506331A (en) | 1999-06-08 |
| EP0828820A4 (en) | 2002-12-04 |
| EP0828820B1 (en) | 2005-12-28 |
| AU5970196A (en) | 1996-12-18 |
| NZ309512A (en) | 1999-06-29 |
| EP0828820A1 (en) | 1998-03-18 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |