ATE316154T1 - Nachweisverfahren der nukleinsäurehybridisierung - Google Patents

Nachweisverfahren der nukleinsäurehybridisierung

Info

Publication number
ATE316154T1
ATE316154T1 AT02758903T AT02758903T ATE316154T1 AT E316154 T1 ATE316154 T1 AT E316154T1 AT 02758903 T AT02758903 T AT 02758903T AT 02758903 T AT02758903 T AT 02758903T AT E316154 T1 ATE316154 T1 AT E316154T1
Authority
AT
Austria
Prior art keywords
nucleic acid
detection method
working electrode
electrode surface
acid hybridization
Prior art date
Application number
AT02758903T
Other languages
English (en)
Inventor
Jong-Hoon Hahn
Nokyoung Park
Original Assignee
Posco
Postech Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from KR10-2002-0043389A external-priority patent/KR100467778B1/ko
Application filed by Posco, Postech Foundation filed Critical Posco
Application granted granted Critical
Publication of ATE316154T1 publication Critical patent/ATE316154T1/de

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/28Electrolytic cell components
    • G01N27/30Electrodes, e.g. test electrodes; Half-cells
    • G01N27/327Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
    • G01N27/3275Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
    • G01N27/3277Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction being a redox reaction, e.g. detection by cyclic voltammetry

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
AT02758903T 2001-07-25 2002-07-25 Nachweisverfahren der nukleinsäurehybridisierung ATE316154T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR20010044971 2001-07-25
KR10-2002-0043389A KR100467778B1 (ko) 2001-07-25 2002-07-23 핵산 혼성화 반응의 검출방법

Publications (1)

Publication Number Publication Date
ATE316154T1 true ATE316154T1 (de) 2006-02-15

Family

ID=36578913

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02758903T ATE316154T1 (de) 2001-07-25 2002-07-25 Nachweisverfahren der nukleinsäurehybridisierung

Country Status (7)

Country Link
EP (1) EP1409741B1 (de)
JP (1) JP4098715B2 (de)
CN (1) CN1254550C (de)
AT (1) ATE316154T1 (de)
DE (2) DE60208801D1 (de)
ES (1) ES2253551T3 (de)
WO (1) WO2003010338A1 (de)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG151333A1 (en) * 2004-04-01 2009-04-30 Univ Nanyang Addressable chem/bio chip array
US7618777B2 (en) 2005-03-16 2009-11-17 Agilent Technologies, Inc. Composition and method for array hybridization
JP2006337153A (ja) * 2005-06-01 2006-12-14 Toppan Printing Co Ltd Dna合成判定装置、核酸タンク装置、dnaチップ装置及びdna判定システム
DE102006017153B3 (de) * 2006-04-04 2007-08-30 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Verfahren zur Herstellung von Oberflächenstrukturen und Element mit Oberflächenstruktur zur Verwendung für Biosensoren oder die Herstellung von Zellleitstrukturen
KR20100025328A (ko) * 2008-08-27 2010-03-09 삼성전자주식회사 이중가닥 영역과 말단 단일가닥 영역을 포함하는 이중가닥 핵산 프로브가 고정된 마이크로어레이를 제조하는 방법
CN101974624A (zh) * 2010-10-11 2011-02-16 福建国际旅行卫生保健中心 一种基于电化学dna生物传感器的军团菌检测新方法及其电化学dna生物传感器
CN110568036A (zh) * 2019-08-16 2019-12-13 成都理工大学 一种基于核酸染料的电化学检测汞离子的方法
JP2023502472A (ja) 2019-11-19 2023-01-24 サーマル,インコーポレイテッド Sars-cov-2を検出する方法およびデバイス

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2573443B2 (ja) * 1990-09-28 1997-01-22 株式会社東芝 遺伝子検出法
US6051380A (en) * 1993-11-01 2000-04-18 Nanogen, Inc. Methods and procedures for molecular biological analysis and diagnostics
US6017696A (en) * 1993-11-01 2000-01-25 Nanogen, Inc. Methods for electronic stringency control for molecular biological analysis and diagnostics
JP3847745B2 (ja) * 1994-04-19 2006-11-22 ザ ユニヴァーシティ オブ ノース カロライナ アット チャペル ヒル 核酸ハイブリッド形成の電気化学的検出
US5968745A (en) * 1995-06-27 1999-10-19 The University Of North Carolina At Chapel Hill Polymer-electrodes for detecting nucleic acid hybridization and method of use thereof
JP3515381B2 (ja) * 1998-09-10 2004-04-05 株式会社東芝 塩基配列検出装置および方法
JP2001013103A (ja) * 1999-04-28 2001-01-19 Fuji Photo Film Co Ltd 走査型電気化学顕微鏡による試料核酸断片の検出方法および定量方法
AU4975300A (en) * 1999-04-30 2000-11-17 Qualicon, Inc. Dna melting curve analysis
AU5253899A (en) * 1999-08-04 2001-03-05 Andcare, Inc. A printed circuit board, biosensor and method of using same
JP2001183367A (ja) * 1999-12-27 2001-07-06 Fuji Photo Film Co Ltd 固相担体表面へのdna断片の固定方法及びdnaチップ

Also Published As

Publication number Publication date
JP2004537050A (ja) 2004-12-09
ES2253551T3 (es) 2006-06-01
DE60208801T2 (de) 2006-08-31
WO2003010338A1 (en) 2003-02-06
DE60208801T8 (de) 2007-09-20
EP1409741A1 (de) 2004-04-21
CN1558957A (zh) 2004-12-29
JP4098715B2 (ja) 2008-06-11
DE60208801D1 (de) 2006-04-06
CN1254550C (zh) 2006-05-03
EP1409741B1 (de) 2006-01-18

Similar Documents

Publication Publication Date Title
ATE552350T1 (de) Elektrokatalytischer nukleinsäurehybridisierungsnachweis
EP1211507A3 (de) Sensor zur Bestimmung von Nukleinsäuren
EP1134294A3 (de) Verfahren zur Synthese eines immobilisierten Nukleinsäurestranges
ATE440148T1 (de) Verbesserte sequenzierung mittels hybridisierung durch verwendung von sondengemischen
WO2005108612A3 (en) Nanoscale biosensor device, system and technique
ATE427999T1 (de) Erkennung von nukleinsaurepolymorphismen
EP1425419A4 (de) Elektrochemischer sensor unter verwendung interkalierender, redoxaktiver gruppen
EA200100672A1 (ru) Метод электрохимического определения событий гибридизации олигомеров нуклеиновой кислоты
WO2003019147A3 (en) Enzyme-amplified redox microarray detection process
IL173314A0 (en) Determining the sequence of a polynucleotide
ATE316154T1 (de) Nachweisverfahren der nukleinsäurehybridisierung
WO2005047468A3 (en) Improved methods for detecting and measuring specific nucleic acid sequences
WO2003093429A3 (en) Nucleic acid reactions using labels with different redox potentials
WO2004099433A3 (en) Electrochemical method to measure dna attachment to an electrode surface in the presence of molecular oxygen
WO2003085082A3 (en) Nucleic acid reactions using labels with different redox potentials
TW200506067A (en) Method of detecting target nucleotide sequence, detection target structure to be used in embodying the method, process for producing the same and assay kit for detecting target nucleotide sequence
ATE445715T1 (de) Elektrokatalytischer nukleinsäurehybridisierungsnachweis
ATE434056T1 (de) Verfahren zur markierung und analyse von nukleinsäuren
ATE449868T1 (de) Methoden, testsätze und kompositionen zur detektion und quantifizierung von zielsequenzen
CA2441180A1 (en) Method for electrochemical detection of nucleobase pair complementarity
Luo et al. Real time electrochemical monitoring of PCR amplicons using electroactive hydrolysis probe
Brisset et al. The first automated synthesis of ferrocene‐labelled phosphorothioate DNA probe: A new potential tool for the fabrication of DNA microarrays
WO2004044240A3 (de) Verfahren zum parallelen nachweis unterschiedlicher nukleinsäuren
EP1323835A3 (de) Methode zur Bestimmung einer Biospezies in einer Testprobe und dafür verwendeter Testsatz
EP1548103A4 (de) Hybridisierung-wahrnehmendes teil, sensor-chip und hybridisierungsverfahren

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties