ATE316154T1 - Nachweisverfahren der nukleinsäurehybridisierung - Google Patents
Nachweisverfahren der nukleinsäurehybridisierungInfo
- Publication number
- ATE316154T1 ATE316154T1 AT02758903T AT02758903T ATE316154T1 AT E316154 T1 ATE316154 T1 AT E316154T1 AT 02758903 T AT02758903 T AT 02758903T AT 02758903 T AT02758903 T AT 02758903T AT E316154 T1 ATE316154 T1 AT E316154T1
- Authority
- AT
- Austria
- Prior art keywords
- nucleic acid
- detection method
- working electrode
- electrode surface
- acid hybridization
- Prior art date
Links
- 238000001514 detection method Methods 0.000 title abstract 2
- 238000007899 nucleic acid hybridization Methods 0.000 title 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 4
- 102000039446 nucleic acids Human genes 0.000 abstract 4
- 108020004707 nucleic acids Proteins 0.000 abstract 4
- 239000000523 sample Substances 0.000 abstract 3
- 239000000463 material Substances 0.000 abstract 2
- 238000002848 electrochemical method Methods 0.000 abstract 1
- 238000009396 hybridization Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
- G01N27/3275—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
- G01N27/3277—Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction being a redox reaction, e.g. detection by cyclic voltammetry
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR20010044971 | 2001-07-25 | ||
| KR10-2002-0043389A KR100467778B1 (ko) | 2001-07-25 | 2002-07-23 | 핵산 혼성화 반응의 검출방법 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE316154T1 true ATE316154T1 (de) | 2006-02-15 |
Family
ID=36578913
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT02758903T ATE316154T1 (de) | 2001-07-25 | 2002-07-25 | Nachweisverfahren der nukleinsäurehybridisierung |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP1409741B1 (de) |
| JP (1) | JP4098715B2 (de) |
| CN (1) | CN1254550C (de) |
| AT (1) | ATE316154T1 (de) |
| DE (2) | DE60208801D1 (de) |
| ES (1) | ES2253551T3 (de) |
| WO (1) | WO2003010338A1 (de) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SG151333A1 (en) * | 2004-04-01 | 2009-04-30 | Univ Nanyang | Addressable chem/bio chip array |
| US7618777B2 (en) | 2005-03-16 | 2009-11-17 | Agilent Technologies, Inc. | Composition and method for array hybridization |
| JP2006337153A (ja) * | 2005-06-01 | 2006-12-14 | Toppan Printing Co Ltd | Dna合成判定装置、核酸タンク装置、dnaチップ装置及びdna判定システム |
| DE102006017153B3 (de) * | 2006-04-04 | 2007-08-30 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Verfahren zur Herstellung von Oberflächenstrukturen und Element mit Oberflächenstruktur zur Verwendung für Biosensoren oder die Herstellung von Zellleitstrukturen |
| KR20100025328A (ko) * | 2008-08-27 | 2010-03-09 | 삼성전자주식회사 | 이중가닥 영역과 말단 단일가닥 영역을 포함하는 이중가닥 핵산 프로브가 고정된 마이크로어레이를 제조하는 방법 |
| CN101974624A (zh) * | 2010-10-11 | 2011-02-16 | 福建国际旅行卫生保健中心 | 一种基于电化学dna生物传感器的军团菌检测新方法及其电化学dna生物传感器 |
| CN110568036A (zh) * | 2019-08-16 | 2019-12-13 | 成都理工大学 | 一种基于核酸染料的电化学检测汞离子的方法 |
| JP2023502472A (ja) | 2019-11-19 | 2023-01-24 | サーマル,インコーポレイテッド | Sars-cov-2を検出する方法およびデバイス |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2573443B2 (ja) * | 1990-09-28 | 1997-01-22 | 株式会社東芝 | 遺伝子検出法 |
| US6051380A (en) * | 1993-11-01 | 2000-04-18 | Nanogen, Inc. | Methods and procedures for molecular biological analysis and diagnostics |
| US6017696A (en) * | 1993-11-01 | 2000-01-25 | Nanogen, Inc. | Methods for electronic stringency control for molecular biological analysis and diagnostics |
| JP3847745B2 (ja) * | 1994-04-19 | 2006-11-22 | ザ ユニヴァーシティ オブ ノース カロライナ アット チャペル ヒル | 核酸ハイブリッド形成の電気化学的検出 |
| US5968745A (en) * | 1995-06-27 | 1999-10-19 | The University Of North Carolina At Chapel Hill | Polymer-electrodes for detecting nucleic acid hybridization and method of use thereof |
| JP3515381B2 (ja) * | 1998-09-10 | 2004-04-05 | 株式会社東芝 | 塩基配列検出装置および方法 |
| JP2001013103A (ja) * | 1999-04-28 | 2001-01-19 | Fuji Photo Film Co Ltd | 走査型電気化学顕微鏡による試料核酸断片の検出方法および定量方法 |
| AU4975300A (en) * | 1999-04-30 | 2000-11-17 | Qualicon, Inc. | Dna melting curve analysis |
| AU5253899A (en) * | 1999-08-04 | 2001-03-05 | Andcare, Inc. | A printed circuit board, biosensor and method of using same |
| JP2001183367A (ja) * | 1999-12-27 | 2001-07-06 | Fuji Photo Film Co Ltd | 固相担体表面へのdna断片の固定方法及びdnaチップ |
-
2002
- 2002-07-25 EP EP02758903A patent/EP1409741B1/de not_active Expired - Lifetime
- 2002-07-25 JP JP2003515688A patent/JP4098715B2/ja not_active Expired - Fee Related
- 2002-07-25 ES ES02758903T patent/ES2253551T3/es not_active Expired - Lifetime
- 2002-07-25 DE DE60208801A patent/DE60208801D1/de not_active Expired - Lifetime
- 2002-07-25 DE DE60208801T patent/DE60208801T8/de active Active
- 2002-07-25 AT AT02758903T patent/ATE316154T1/de not_active IP Right Cessation
- 2002-07-25 CN CN02818727.XA patent/CN1254550C/zh not_active Expired - Fee Related
- 2002-07-25 WO PCT/KR2002/001402 patent/WO2003010338A1/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| JP2004537050A (ja) | 2004-12-09 |
| ES2253551T3 (es) | 2006-06-01 |
| DE60208801T2 (de) | 2006-08-31 |
| WO2003010338A1 (en) | 2003-02-06 |
| DE60208801T8 (de) | 2007-09-20 |
| EP1409741A1 (de) | 2004-04-21 |
| CN1558957A (zh) | 2004-12-29 |
| JP4098715B2 (ja) | 2008-06-11 |
| DE60208801D1 (de) | 2006-04-06 |
| CN1254550C (zh) | 2006-05-03 |
| EP1409741B1 (de) | 2006-01-18 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |