ATE440148T1 - Verbesserte sequenzierung mittels hybridisierung durch verwendung von sondengemischen - Google Patents

Verbesserte sequenzierung mittels hybridisierung durch verwendung von sondengemischen

Info

Publication number
ATE440148T1
ATE440148T1 AT00903186T AT00903186T ATE440148T1 AT E440148 T1 ATE440148 T1 AT E440148T1 AT 00903186 T AT00903186 T AT 00903186T AT 00903186 T AT00903186 T AT 00903186T AT E440148 T1 ATE440148 T1 AT E440148T1
Authority
AT
Austria
Prior art keywords
pools
probes
via hybridization
probe mixtures
sequencing via
Prior art date
Application number
AT00903186T
Other languages
English (en)
Inventor
Radoje Drmanac
Snezana Drmanac
David Kita
Cory Cooke
Chongjun Xu
Original Assignee
Callida Genomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Callida Genomics Inc filed Critical Callida Genomics Inc
Application granted granted Critical
Publication of ATE440148T1 publication Critical patent/ATE440148T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
AT00903186T 1999-01-06 2000-01-06 Verbesserte sequenzierung mittels hybridisierung durch verwendung von sondengemischen ATE440148T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11528499P 1999-01-06 1999-01-06
PCT/US2000/000458 WO2000040758A2 (en) 1999-01-06 2000-01-06 Enhanced sequencing by hybridization using pools of probes

Publications (1)

Publication Number Publication Date
ATE440148T1 true ATE440148T1 (de) 2009-09-15

Family

ID=22360367

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00903186T ATE440148T1 (de) 1999-01-06 2000-01-06 Verbesserte sequenzierung mittels hybridisierung durch verwendung von sondengemischen

Country Status (6)

Country Link
US (4) US6864052B1 (de)
EP (2) EP1144684B1 (de)
AT (1) ATE440148T1 (de)
AU (1) AU2496900A (de)
DE (1) DE60042775D1 (de)
WO (1) WO2000040758A2 (de)

Families Citing this family (206)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6780591B2 (en) 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US20030022207A1 (en) * 1998-10-16 2003-01-30 Solexa, Ltd. Arrayed polynucleotides and their use in genome analysis
US20040106110A1 (en) * 1998-07-30 2004-06-03 Solexa, Ltd. Preparation of polynucleotide arrays
US20100130368A1 (en) * 1998-07-30 2010-05-27 Shankar Balasubramanian Method and system for sequencing polynucleotides
EP1144684B1 (de) * 1999-01-06 2009-08-19 Callida Genomics, Inc. Verbesserte sequenzierung mittels hybridisierung durch verwendung von sondengemischen
AU5497100A (en) * 1999-06-19 2001-01-09 Hyseq, Inc. Improved methods of sequence assembly in sequencing by hybridization
US7501245B2 (en) 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US7504213B2 (en) 1999-07-09 2009-03-17 Agilent Technologies, Inc. Methods and apparatus for preparing arrays comprising features having degenerate biopolymers
CA2416952A1 (en) * 2000-07-27 2002-02-07 The Australian National University Combinatorial probes and uses therefor
US9708358B2 (en) 2000-10-06 2017-07-18 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
EP3034627B1 (de) 2000-10-06 2019-01-30 The Trustees of Columbia University in the City of New York Massives paralleles verfahren zum entschlüsseln von dna und rna
JP2004523243A (ja) 2001-03-12 2004-08-05 カリフォルニア インスティチュート オブ テクノロジー 非同期性塩基伸長によってポリヌクレオチド配列を分析するための方法および装置
EP2801624B1 (de) * 2001-03-16 2019-03-06 Singular Bio, Inc Arrays und Verfahren zur Verwendung
US7138506B2 (en) * 2001-05-09 2006-11-21 Genetic Id, Na, Inc. Universal microarray system
US20040009484A1 (en) * 2002-07-11 2004-01-15 Wolber Paul K. Methods for evaluating oligonucleotide probes of variable length
WO2004029586A1 (en) * 2002-09-27 2004-04-08 Nimblegen Systems, Inc. Microarray with hydrophobic barriers
CN1791682B (zh) * 2003-02-26 2013-05-22 凯利达基因组股份有限公司 通过杂交进行的随机阵列dna分析
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
ATE463584T1 (de) 2004-02-19 2010-04-15 Helicos Biosciences Corp Verfahren zur analyse von polynukleotidsequenzen
EP1766090B1 (de) 2004-05-25 2011-04-27 Helicos Biosciences Corporation Verfahren zur nukleinsäureimmobilisierung
US7476734B2 (en) 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
US7220549B2 (en) 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US20060226140A1 (en) * 2005-01-25 2006-10-12 Mr. Sake, Llc Heated tray
US7482120B2 (en) 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
US20060240443A1 (en) * 2005-04-20 2006-10-26 Combimatrix Corporation Microarray-based single nucleotide polymorphism, sequencing, and gene expression assay method
DK1907571T3 (en) 2005-06-15 2017-08-21 Complete Genomics Inc NUCLEIC ACID ANALYSIS USING INCIDENTAL MIXTURES OF NON-OVERLAPPING FRAGMENTS
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
EP1951900B1 (de) 2005-10-07 2016-06-15 Callida Genomics, Inc. Selbstzusammengesetzte einzelmolekül-arrays und verwendungen davon
US7960104B2 (en) * 2005-10-07 2011-06-14 Callida Genomics, Inc. Self-assembled single molecule arrays and uses thereof
US20070168197A1 (en) * 2006-01-18 2007-07-19 Nokia Corporation Audio coding
CN101432439B (zh) * 2006-02-24 2013-07-24 考利达基因组股份有限公司 Dna阵列上的高通量基因组测序
SG170028A1 (en) * 2006-02-24 2011-04-29 Callida Genomics Inc High throughput genome sequencing on dna arrays
US7397546B2 (en) 2006-03-08 2008-07-08 Helicos Biosciences Corporation Systems and methods for reducing detected intensity non-uniformity in a laser beam
WO2008070352A2 (en) * 2006-10-27 2008-06-12 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
US20090075343A1 (en) 2006-11-09 2009-03-19 Complete Genomics, Inc. Selection of dna adaptor orientation by nicking
US20090105961A1 (en) * 2006-11-09 2009-04-23 Complete Genomics, Inc. Methods of nucleic acid identification in large-scale sequencing
WO2008130394A2 (en) * 2006-11-30 2008-10-30 The Regents Of The University Of California Array for detecting microbes
GB2457402B (en) 2006-12-01 2011-10-19 Univ Columbia Four-color DNA sequencing by synthesis using cleavable fluorescent nucleotide reversible terminators
US11940413B2 (en) 2007-02-05 2024-03-26 IsoPlexis Corporation Methods and devices for sequencing nucleic acids in smaller batches
WO2009052214A2 (en) * 2007-10-15 2009-04-23 Complete Genomics, Inc. Sequence analysis using decorated nucleic acids
EP2207900B1 (de) 2007-10-19 2015-04-29 The Trustees of Columbia University in the City of New York Konstruktion und synthese spaltbarer fluoreszenznukleotide als reversible terminatoren für die dna-sequenzierung mittels synthese
EP2209911B1 (de) 2007-10-19 2013-10-16 The Trustees of Columbia University in the City of New York Dna-sequenzierung mit reversiblen nicht-fluoreszenz-nukleotid-terminatoren und mit einer spaltbaren markierung modifizierten nukleotidterminatoren und eine deoxyinosineverbindung mit einer reversiblen terminatorgruppe
US8518640B2 (en) * 2007-10-29 2013-08-27 Complete Genomics, Inc. Nucleic acid sequencing and process
US20090263872A1 (en) * 2008-01-23 2009-10-22 Complete Genomics Inc. Methods and compositions for preventing bias in amplification and sequencing reactions
US7897344B2 (en) * 2007-11-06 2011-03-01 Complete Genomics, Inc. Methods and oligonucleotide designs for insertion of multiple adaptors into library constructs
US8415099B2 (en) 2007-11-05 2013-04-09 Complete Genomics, Inc. Efficient base determination in sequencing reactions
CN105349647B (zh) 2007-10-30 2020-08-28 完整基因有限公司 用于核酸高通量测序的方法
WO2009061840A1 (en) * 2007-11-05 2009-05-14 Complete Genomics, Inc. Methods and oligonucleotide designs for insertion of multiple adaptors employing selective methylation
WO2009073629A2 (en) 2007-11-29 2009-06-11 Complete Genomics, Inc. Efficient shotgun sequencing methods
US9551026B2 (en) 2007-12-03 2017-01-24 Complete Genomincs, Inc. Method for nucleic acid detection using voltage enhancement
US8592150B2 (en) 2007-12-05 2013-11-26 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
CN103290106B (zh) 2007-12-05 2015-07-29 考利达基因组股份有限公司 测序反应中碱基的有效确定
WO2009092035A2 (en) 2008-01-17 2009-07-23 Sequenom, Inc. Methods and compositions for the analysis of biological molecules
WO2009097368A2 (en) 2008-01-28 2009-08-06 Complete Genomics, Inc. Methods and compositions for efficient base calling in sequencing reactions
US9017973B2 (en) 2008-03-19 2015-04-28 Intelligent Biosystems, Inc. Methods and compositions for incorporating nucleotides
WO2009132028A1 (en) * 2008-04-21 2009-10-29 Complete Genomics, Inc. Array structures for nucleic acid detection
EP3216874A1 (de) 2008-09-05 2017-09-13 TOMA Biosciences, Inc. Verfahren zum stratifizieren und annotieren der behandlungsoptionen eines krebsmedikaments
EP2394165A4 (de) * 2009-02-03 2013-12-11 Complete Genomics Inc Zuordnung von oligomersequenzen
US8615365B2 (en) * 2009-02-03 2013-12-24 Complete Genomics, Inc. Oligomer sequences mapping
US8738296B2 (en) * 2009-02-03 2014-05-27 Complete Genomics, Inc. Indexing a reference sequence for oligomer sequence mapping
EP2511843B1 (de) * 2009-04-29 2016-12-21 Complete Genomics, Inc. Verfahren und System zum Aufrufen von Variationen in einer Polynukleotidprobensequenz in Bezug auf eine Referenzpolynukleotidsequenz
EP2425240A4 (de) 2009-04-30 2012-12-12 Good Start Genetics Inc Verfahren und zusammensetzung zur bewertung genetischer marker
US12129514B2 (en) 2009-04-30 2024-10-29 Molecular Loop Biosolutions, Llc Methods and compositions for evaluating genetic markers
US9085798B2 (en) 2009-04-30 2015-07-21 Prognosys Biosciences, Inc. Nucleic acid constructs and methods of use
US9524369B2 (en) 2009-06-15 2016-12-20 Complete Genomics, Inc. Processing and analysis of complex nucleic acid sequence data
CN102459592B (zh) 2009-06-15 2017-04-05 考利达基因组股份有限公司 用于长片段阅读测序的方法和组合物
WO2011050340A1 (en) * 2009-10-23 2011-04-28 Life Technologies Corporation Systems and methods for error correction in dna sequencing
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
KR101866401B1 (ko) 2010-04-05 2018-06-11 프로그노시스 바이오사이언스, 인코포레이티드 공간적으로 엔코딩된 생물학적 검정
US8774494B2 (en) 2010-04-30 2014-07-08 Complete Genomics, Inc. Method and system for accurate alignment and registration of array for DNA sequencing
US10167508B2 (en) 2010-08-06 2019-01-01 Ariosa Diagnostics, Inc. Detection of genetic abnormalities
US20130040375A1 (en) 2011-08-08 2013-02-14 Tandem Diagnotics, Inc. Assay systems for genetic analysis
US11203786B2 (en) 2010-08-06 2021-12-21 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US20140342940A1 (en) 2011-01-25 2014-11-20 Ariosa Diagnostics, Inc. Detection of Target Nucleic Acids using Hybridization
US20120034603A1 (en) 2010-08-06 2012-02-09 Tandem Diagnostics, Inc. Ligation-based detection of genetic variants
US10533223B2 (en) 2010-08-06 2020-01-14 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US20130261003A1 (en) 2010-08-06 2013-10-03 Ariosa Diagnostics, In. Ligation-based detection of genetic variants
US11031095B2 (en) 2010-08-06 2021-06-08 Ariosa Diagnostics, Inc. Assay systems for determination of fetal copy number variation
US8700338B2 (en) 2011-01-25 2014-04-15 Ariosa Diagnosis, Inc. Risk calculation for evaluation of fetal aneuploidy
US9671344B2 (en) 2010-08-31 2017-06-06 Complete Genomics, Inc. High-density biochemical array chips with asynchronous tracks for alignment correction by moiré averaging
US9880089B2 (en) 2010-08-31 2018-01-30 Complete Genomics, Inc. High-density devices with synchronous tracks for quad-cell based alignment correction
AU2011305445B2 (en) * 2010-09-24 2017-03-16 The Board Of Trustees Of The Leland Stanford Junior University Direct capture, amplification and sequencing of target DNA using immobilized primers
US8725422B2 (en) 2010-10-13 2014-05-13 Complete Genomics, Inc. Methods for estimating genome-wide copy number variations
US9163281B2 (en) 2010-12-23 2015-10-20 Good Start Genetics, Inc. Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction
US9994897B2 (en) 2013-03-08 2018-06-12 Ariosa Diagnostics, Inc. Non-invasive fetal sex determination
US10131947B2 (en) 2011-01-25 2018-11-20 Ariosa Diagnostics, Inc. Noninvasive detection of fetal aneuploidy in egg donor pregnancies
US8756020B2 (en) 2011-01-25 2014-06-17 Ariosa Diagnostics, Inc. Enhanced risk probabilities using biomolecule estimations
US20120190021A1 (en) 2011-01-25 2012-07-26 Aria Diagnostics, Inc. Detection of genetic abnormalities
US11270781B2 (en) 2011-01-25 2022-03-08 Ariosa Diagnostics, Inc. Statistical analysis for non-invasive sex chromosome aneuploidy determination
WO2012118745A1 (en) 2011-02-28 2012-09-07 Arnold Oliphant Assay systems for detection of aneuploidy and sex determination
US20120258871A1 (en) 2011-04-08 2012-10-11 Prognosys Biosciences, Inc. Peptide constructs and assay systems
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
WO2012162429A2 (en) 2011-05-23 2012-11-29 The Trustees Of Columbia University In The City Of New York Dna sequencing by synthesis using raman and infrared spectroscopy detection
US8712697B2 (en) 2011-09-07 2014-04-29 Ariosa Diagnostics, Inc. Determination of copy number variations using binomial probability calculations
EP2768983A4 (de) 2011-10-17 2015-06-03 Good Start Genetics Inc Analyseverfahren
EP2769007B1 (de) 2011-10-19 2016-12-07 Nugen Technologies, Inc. Zusammensetzungen und verfahren zur direktionalen amplifikation und sequenzierung von nukleinsäuren
US10837879B2 (en) 2011-11-02 2020-11-17 Complete Genomics, Inc. Treatment for stabilizing nucleic acid arrays
CN105861487B (zh) 2012-01-26 2020-05-05 纽亘技术公司 用于靶向核酸序列富集和高效文库产生的组合物和方法
US9803239B2 (en) 2012-03-29 2017-10-31 Complete Genomics, Inc. Flow cells for high density array chips
US8209130B1 (en) 2012-04-04 2012-06-26 Good Start Genetics, Inc. Sequence assembly
US8812422B2 (en) 2012-04-09 2014-08-19 Good Start Genetics, Inc. Variant database
US10227635B2 (en) 2012-04-16 2019-03-12 Molecular Loop Biosolutions, Llc Capture reactions
WO2013166517A1 (en) 2012-05-04 2013-11-07 Complete Genomics, Inc. Methods for determining absolute genome-wide copy number variations of complex tumors
US10289800B2 (en) 2012-05-21 2019-05-14 Ariosa Diagnostics, Inc. Processes for calculating phased fetal genomic sequences
US9884893B2 (en) 2012-05-21 2018-02-06 Distributed Bio, Inc. Epitope focusing by variable effective antigen surface concentration
WO2013177220A1 (en) 2012-05-21 2013-11-28 The Scripps Research Institute Methods of sample preparation
US9488823B2 (en) 2012-06-07 2016-11-08 Complete Genomics, Inc. Techniques for scanned illumination
US9628676B2 (en) 2012-06-07 2017-04-18 Complete Genomics, Inc. Imaging systems with movable scan mirrors
CA2877094A1 (en) 2012-06-18 2013-12-27 Nugen Technologies, Inc. Compositions and methods for negative selection of non-desired nucleic acid sequences
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
CN104583421A (zh) 2012-07-19 2015-04-29 阿瑞奥萨诊断公司 遗传变体的基于多重的顺序连接的检测
EP3901280B1 (de) 2012-10-17 2025-03-12 10x Genomics Sweden AB Verfahren und produkt zur optimierung einer lokalisierten oder räumlichen detektion einer genexpression in einer gewebeprobe
EP2971159B1 (de) 2013-03-14 2019-05-08 Molecular Loop Biosolutions, LLC Verfahren zur analyse von nukleinsäuren
US9328382B2 (en) 2013-03-15 2016-05-03 Complete Genomics, Inc. Multiple tagging of individual long DNA fragments
WO2014144883A1 (en) 2013-03-15 2014-09-18 The Trustees Of Columbia University In The City Of New York Raman cluster tagged molecules for biological imaging
EP2971130A4 (de) 2013-03-15 2016-10-05 Nugen Technologies Inc Sequentielle sequenzierung
CN105431553B (zh) * 2013-05-29 2020-02-07 生物辐射实验室股份有限公司 用于在基于乳液的微流体中测序的系统和方法
WO2014197377A2 (en) 2013-06-03 2014-12-11 Good Start Genetics, Inc. Methods and systems for storing sequence read data
CN105074011B (zh) 2013-06-13 2020-10-02 阿瑞奥萨诊断公司 用于非入侵性性染色体非整倍性确定的统计分析
CN111500680B (zh) 2013-06-25 2024-04-16 普罗格诺西斯生物科学公司 检测样品中生物靶标的空间分布的方法和系统
CN105637099B (zh) 2013-08-23 2020-05-19 深圳华大智造科技有限公司 使用短读段的长片段从头组装
EP3049539B1 (de) 2013-09-25 2018-09-05 Bio-Id Diagnostic Inc. Verfahren für den nachweis von nukleinsäurefragmenten
US10851414B2 (en) 2013-10-18 2020-12-01 Good Start Genetics, Inc. Methods for determining carrier status
US11041203B2 (en) 2013-10-18 2021-06-22 Molecular Loop Biosolutions, Inc. Methods for assessing a genomic region of a subject
JP6525473B2 (ja) 2013-11-13 2019-06-05 ニューゲン テクノロジーズ， インコーポレイテッド 複製物配列決定リードを同定するための組成物および方法
WO2015131107A1 (en) 2014-02-28 2015-09-03 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
WO2015175530A1 (en) 2014-05-12 2015-11-19 Gore Athurva Methods for detecting aneuploidy
WO2016025818A1 (en) 2014-08-15 2016-02-18 Good Start Genetics, Inc. Systems and methods for genetic analysis
US10450562B2 (en) 2014-09-09 2019-10-22 Igenomx International Genomics Corporation Methods and compositions for rapid nucleic acid library preparation
US11408024B2 (en) 2014-09-10 2022-08-09 Molecular Loop Biosciences, Inc. Methods for selectively suppressing non-target sequences
US10429399B2 (en) 2014-09-24 2019-10-01 Good Start Genetics, Inc. Process control for increased robustness of genetic assays
EP3271480B8 (de) 2015-01-06 2022-09-28 Molecular Loop Biosciences, Inc. Abtastung auf strukturelle varianten
JP6929778B2 (ja) 2015-01-15 2021-09-01 グッド スタート ジェネティクス， インコーポレイテッド 着床前遺伝子スクリーニングにおける一塩基多型を用いた品質管理方法
EP4321627A3 (de) 2015-04-10 2024-04-17 10x Genomics Sweden AB Räumlich getrennte multiplex-nukleinsäureanalyse von biologischen proben
US12492430B2 (en) 2017-04-11 2025-12-09 Tecan Genomics, Inc. Library quantitation and qualification
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
ES2947437T3 (es) 2018-05-08 2023-08-09 Mgi Tech Co Ltd Creación de Códigos de barra compartidos en el ADN, en un solo tubo con perlas, para la secuenciación, haplotipado y ensamblaje preciso y rentable
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
EP3894585A2 (de) 2018-12-10 2021-10-20 10X Genomics, Inc. Erzeugung von erfassungssonden zur räumlichen analyse
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
EP3976820A1 (de) 2019-05-30 2022-04-06 10X Genomics, Inc. Verfahren zum nachweis der räumlichen heterogenität einer biologischen probe
EP4025711A2 (de) 2019-11-08 2022-07-13 10X Genomics, Inc. Erhöhung der spezifität einer analytbindung
WO2021091611A1 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
CN121022991A (zh) 2019-12-23 2025-11-28 10X基因组学有限公司 使用rna模板化连接进行空间分析的方法
EP4081656A1 (de) 2019-12-23 2022-11-02 10X Genomics, Inc. Zusammensetzungen und verfahren zur verwendung von fixierten biologischen proben bei partitionsbasierten tests
EP4752521A3 (de) 2019-12-23 2026-08-12 10X Genomics, Inc. Reversible fixierreagenzien und verfahren zur verwendung davon
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
EP3851542A1 (de) 2020-01-20 2021-07-21 Tecan Genomics, Inc. Verarmung von reichlich uninformativen sequenzen
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
US11821035B1 (en) 2020-01-29 2023-11-21 10X Genomics, Inc. Compositions and methods of making gene expression libraries
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
CN113275053A (zh) 2020-02-03 2021-08-20 帝肯基因组学公司 试剂存储系统
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US12129516B2 (en) 2020-02-07 2024-10-29 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
US12399123B1 (en) 2020-02-14 2025-08-26 10X Genomics, Inc. Spatial targeting of analytes
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
CN116157533A (zh) 2020-02-21 2023-05-23 10X基因组学有限公司 使用杂交方法捕获遗传靶标
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
US11768175B1 (en) 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
EP4139485B1 (de) 2020-04-22 2023-09-06 10X Genomics, Inc. Verfahren zur räumlichen analyse unter verwendung von gezieltem rna-abbau
US12416603B2 (en) 2020-05-19 2025-09-16 10X Genomics, Inc. Electrophoresis cassettes and instrumentation
EP4153775B1 (de) 2020-05-22 2024-07-24 10X Genomics, Inc. Simultane räumlich-zeitliche messung der genexpression und der zellaktivität
AU2021275906A1 (en) 2020-05-22 2022-12-22 10X Genomics, Inc. Spatial analysis to detect sequence variants
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
US12265079B1 (en) 2020-06-02 2025-04-01 10X Genomics, Inc. Systems and methods for detecting analytes from captured single biological particles
EP4600376A3 (de) 2020-06-02 2025-10-22 10X Genomics, Inc. Räumliche transkriptomik für antigenrezeptoren
EP4025692A2 (de) 2020-06-02 2022-07-13 10X Genomics, Inc. Nukleinsäure-bibliotheksverfahren
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
EP4421186B1 (de) 2020-06-08 2025-08-13 10X Genomics, Inc. Verfahren zur bestimmung eines chirurgischen randes und verfahren zur verwendung davon
EP4446430A3 (de) 2020-06-10 2024-12-18 10X Genomics, Inc. Verfahren zur bestimmung einer position eines analyten in einer biologischen probe
EP4164796A4 (de) 2020-06-10 2024-03-06 10x Genomics, Inc. Flüssigkeitsabgabeverfahren
US12435363B1 (en) 2020-06-10 2025-10-07 10X Genomics, Inc. Materials and methods for spatial transcriptomics
EP4450639B1 (de) 2020-06-25 2025-10-15 10X Genomics, Inc. Räumliche analyse der dna-methylierung
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
US12553898B1 (en) 2020-08-10 2026-02-17 10X Genomics, Inc. Fluorescent hybridization of antibody-oligonucleotide for multiplexing and signal amplification
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
CN116194590A (zh) 2020-09-18 2023-05-30 10X基因组学有限公司 样品处理设备和图像配准方法
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
WO2022140028A1 (en) 2020-12-21 2022-06-30 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
ES2989523T3 (es) 2021-02-19 2024-11-26 10X Genomics Inc Método de uso de un dispositivo modular de soporte para ensayos
EP4524261B1 (de) 2021-03-18 2026-07-22 10X Genomics, Inc. Multiplex-erfassung von gen- und proteinexpression aus einer biologischen probe
EP4305196B1 (de) 2021-04-14 2025-04-02 10X Genomics, Inc. Verfahren zur messung der fehllokalisierung eines analyten
EP4320271B1 (de) 2021-05-06 2025-03-19 10X Genomics, Inc. Verfahren zur erhöhung der auflösung von räumlicher analyse
ES3030033T3 (en) 2021-06-03 2025-06-26 10X Genomics Inc Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
US12553805B2 (en) 2021-08-02 2026-02-17 10X Genomics, Inc. Methods of preserving a biological sample
WO2023034489A1 (en) 2021-09-01 2023-03-09 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array
EP4419707A1 (de) 2021-11-10 2024-08-28 10X Genomics, Inc. Verfahren, zusammensetzungen und kits zur bestimmung der position eines analyten in einer biologischen probe
EP4305195A2 (de) 2021-12-01 2024-01-17 10X Genomics, Inc. Verfahren, zusammensetzungen und systeme für verbesserten in-situ-nachweis von analyten und raumanalyse
EP4441711A1 (de) 2021-12-20 2024-10-09 10X Genomics, Inc. Selbsttest für eine pathologie-/histologie-objektträgerbildgebungsvorrichtung
US12637706B2 (en) 2022-03-23 2026-05-26 10X Genomics, Inc. Methods and compositions related to microsphere surface gelation
EP4685239A1 (de) 2022-11-09 2026-01-28 10X Genomics, Inc. Verfahren, zusammensetzungen und kits zur bestimmung der position mehrerer analyten in einer biologischen probe
US20260015659A1 (en) * 2024-07-11 2026-01-15 SequLITE Genomics US, Inc. Method for Nucleic Acid Sequence Detection and Sequencing Method

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6270961B1 (en) 1987-04-01 2001-08-07 Hyseq, Inc. Methods and apparatus for DNA sequencing and DNA identification
US5202231A (en) 1987-04-01 1993-04-13 Drmanac Radoje T Method of sequencing of genomes by hybridization of oligonucleotide probes
US5525464A (en) 1987-04-01 1996-06-11 Hyseq, Inc. Method of sequencing by hybridization of oligonucleotide probes
US5002867A (en) * 1988-04-25 1991-03-26 Macevicz Stephen C Nucleic acid sequence determination by multiple mixed oligonucleotide probes
US5744101A (en) 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
WO1995011995A1 (en) * 1993-10-26 1995-05-04 Affymax Technologies N.V. Arrays of nucleic acid probes on biological chips
NZ275194A (en) 1993-09-27 1997-09-22 Arch Dev Corp Method and kit for nucleic acid sequencing
US20020042048A1 (en) * 1997-01-16 2002-04-11 Radoje Drmanac Methods and compositions for detection or quantification of nucleic acid species
EP1012335A4 (de) 1997-08-15 2004-06-09 Hyseq Inc Erfahren und zusammensetzungen zur detektion oder quantifizierung von nukleinsäurespezies
US6355419B1 (en) * 1998-04-27 2002-03-12 Hyseq, Inc. Preparation of pools of nucleic acids based on representation in a sample
EP1144684B1 (de) * 1999-01-06 2009-08-19 Callida Genomics, Inc. Verbesserte sequenzierung mittels hybridisierung durch verwendung von sondengemischen
JP2003527075A (ja) * 1999-03-25 2003-09-16 ハイセック，インコーポレーテッド ハイブリダイゼーションによる配列分析のための、溶液ベースの方法
US8787443B2 (en) 2010-10-05 2014-07-22 Microsoft Corporation Content adaptive deblocking during video encoding and decoding
US11196198B2 (en) 2019-05-03 2021-12-07 Foxconn (Kunshan) Computer Connector Co., Ltd. Card edge connector with improved contacts

Also Published As

Publication number Publication date
US7582431B2 (en) 2009-09-01
US20050191656A1 (en) 2005-09-01
EP1144684A2 (de) 2001-10-17
US20110039731A1 (en) 2011-02-17
US6864052B1 (en) 2005-03-08
EP2145963A1 (de) 2010-01-20
US8034566B2 (en) 2011-10-11
DE60042775D1 (de) 2009-10-01
WO2000040758A2 (en) 2000-07-13
WO2000040758A3 (en) 2000-11-02
EP1144684B1 (de) 2009-08-19
AU2496900A (en) 2000-07-24
US20090280481A1 (en) 2009-11-12

Similar Documents

Publication Publication Date Title
DE60042775D1 (de) Verbesserte sequenzierung mittels hybridisierung durch verwendung von sondengemischen
DE60137722D1 (de) Verwendung von mass-matched nukleotide in der analyse von oligonukleotidmischungen sowie in der hoch-multiplexen nukleinsäuresequenzierung
DK1032705T3 (da) Probearrays og fremgangsmåder til at benytte probearrays til at skelne mellem DNA
GEP20125384B (en) Representations of nucleotide sequences
DK1159453T3 (da) Fremgangsmåde til direkte nukleinsyresekventering
ATE321883T1 (de) DE NOVO ODER ßUNIVERSALERß SEQUENZIERUNGS-ARRAY
GB0507835D0 (en) Method and device for nucleic acid sequencing using a planar wave guide
ATE244259T1 (de) Verwendungen von fluoreszierenden n-nukleosiden und dessen analogen
ATE388243T1 (de) Bestimmung der sequenz eines polynukleotids
ATE266738T1 (de) Genomische multi-loci analyse mittels eines verbesserten verfahrens basierend auf zyklischer dna-sequenzierung
EA200500911A1 (ru) Планшеты и системы для крупномасштабной электропорации и способы их применения
DE60143314D1 (de) Estimmung der präsenz von cryptosporidium organismen in einer testprobe
WO2002016650A3 (en) Compositions and methods for exon profiling
NO20070757L (no) Ny sekvens for forbedring av ekspresjon av nukleinsyre(r)
SG155774A1 (en) Method to generate or determine nucleic acid tags corresponding to the terminal ends of dna molecules using sequences analysis of gene expression (terminal sage)
ATE220427T1 (de) Verfahren zum nachweis von nukleinsäure- zielsequenzen mittels in vitro transkription von einem rna-promoter
DE69929365D1 (de) Systeme und verfahren zum sequenzieren durch hybridation
DE602005009693D1 (de) 2"-nitrobenzyl-modifizierte ribonukleotide
EP1184468A3 (de) Verfahren zur Sequenzierung von Nukleinsäuren mit Sondenarrays
DK1366187T3 (da) Oligonukleotider til mærkning af oligonukleotidprober og proteiner
WO2002018648A3 (en) Analysis of binding interactions
DE60043580D1 (de) MinK-ASSOZIIERTE GENE, BILDUNG VON KALIUM-KANÄLEN SOWIE ASSOZIATION ZU HERZ-ARRHYTMIEN
ATE316154T1 (de) Nachweisverfahren der nukleinsäurehybridisierung
DE60025336D1 (de) Verfahren und kit zur früherkennung von krebs
WO2002046468A3 (en) Method for maldi-tof-ms analysis and/or sequencing of oligonucleotides

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties